Metal aging in soils has been considered an important factor influencing its availability and toxicity to organisms. In this study, we report the influence of 5 years field aging on the nickel (Ni) toxicity to collembolan Folsomia candida based on two different types of soil from Dezhou (DZ) and Qiyang (QY) counties in China. Acute and chronic toxicity of Ni to F. candida was assessed in both freshly spiked and field aging contaminated soils. We found that 5 years field aging increased the EC₅₀ and 2d-LC₅₀ values of Ni to F. candida in the DZ soil, while little influence on the Ni toxicity was observed in the QY soil. There was no adverse effect of the long-term field aging on the Ni toxicity to the survival of F. candida in the two tested soils. In addition, field aging of the two soils impacted differently the water-soluble Ni concentrations, which were significantly correlated to the juvenile production of F. candida based on a logistic model. Our study highlights different effects of long-term field aging on the Ni toxicity to F. candida between divergent types of soil, and this should be taken into account in future toxicity testing and risk assessment practices.

Due to the significant increase in nanoparticle production and especially that of silver nanoparticles over the past decade, the toxicity of silver in both ionic (Ag⁺) and nanoparticulate (AgNPs) form must be studied in detail in order to understand their impact on natural ecosystems. A comparative study of the effect of AgNPs and ionic silver on two independent phototrophic biofilms was conducted in a rotating annular bioreactor (RAB) operating under constant conditions. The concentration of dissolved silver in the inlet solution was progressively increased every 4 days of exposure, from 0.1 to 100 μg L⁻¹. In the course of the 40-day experiment, biofilm samples were collected to determine the evolution of biomass, chlorophyll-a, as well as photosynthetic and heterotrophic enzymatic activities in response to silver addition. Analysis of both dissolved and particulate silver allowed quantification of the distribution coefficient and uptake rate constants. The presence of both AgNPs and Ag⁺ produced significant changes in the biofilm structure, decreasing the relative percentage of Diatomophyceae and Cyanophyceae and increasing the relative percentage of Chlorophyceae. The accumulation capacity of the phototrophic biofilm with respect to ionic silver and the corresponding distribution coefficients were an order of magnitude higher than those of the phototrophic biofilm with respect to AgNPs. Higher levels of AgNPs decreased the biomass from 8.6 ± 0.2 mg cm⁻² for 0–10 μg L⁻¹ AgNPs to 6.0 ± 0.1 mg cm⁻² for 100 μg L⁻¹ added AgNPs, whereas ionic silver did not have any toxic effect on the biofilm growth up to 100 μg L⁻¹ of added Ag⁺. At the same time, AgNPs did not significantly affect the photosynthetic activity of the biofilm surface communities compared to Ag⁺. It can thus be hypothesized that negatively charged AgNPs may travel through the biofilm water channels, thereby affecting the whole biofilm structure. In contrast, positively charged Ag⁺ is bound at the cell surfaces and EPS, thus blocking its further flux within the biofilm layers. On the whole, the phototrophic biofilm demonstrated significant capacities to accumulate silver within the surface layers. The main mechanism to avoid the toxic effects is metal complexation with exopolysaccharides and accumulation within cell walls, especially pronounced under Ag⁺ stress. The significant AgNPs and Ag⁺ uptake capacities of phototrophic biofilm make it a highly resistant ecosystem in silver-polluted river waters.

Aqueous film-forming foams (AFFF) are used to extinguish hydrocarbon fuel fires. Certain AFFF products such as 3M Lightwater contain perfluorooctane sulfonate (PFOS) as the active ingredient which is highly persistent in the environment and is thus globally prevalent. With thousands of tons of soils potentially contaminated with PFOS stockpiled at a number of sites in Australia, the lack of reliable information on bioavailability of this recalcitrant contaminant constrains the application of a risk-based strategy for managing such soils. In this study, the PFOS release pattern from soils collected from the contaminated sites of fire training areas and its bioaccumulation potential in earthworm were investigated. The study was conducted at two temperatures (25 and 37 °C) and 60 % of the maximum water-holding capacity of soils. The greatest release into water was found to occur from the soil having the highest PFOS concentration, 16.17 μg g⁻¹ (Tindal FTA064), thereby demonstrating the role of contaminant loading on release behaviour. The release could also be related to the soil physico-chemical properties. The maximum amount of PFOS was desorbed from the soil with the lowest clay and organic matter content. Bioaccumulation of PFOS in earthworms (Eisensia fetida) as expressed by the bioaccumulation factor (BAF) was found to be highest from soil with the lowest PFOS concentration (RBD soil). The range of BAF found in our study was 1.23 (spiked Tindal SS01 soil) to 13.9 (field contaminated RBD soil). Our study suggests that PFOS could indeed pose a potential risk to ecological safety of soil if present even at concentrations as low as 0.8 μg g⁻¹ since the highest bioaccumulation factor was found to be from such a soil (field contaminated RBD).

Sulfadimethoxine (SDM) is an antibiotic commonly used in concentrated animal feeding operations and released into the environment via manure application on agricultural lands. Transformation of antibiotics in soil impacts the likelihood of their entry to water bodies, uptake by plants, and thus their effect on terrestrial and aquatic organisms. We conducted experiments to incubate SDM in a sandy loam soil in the presence of humification enzymes commonly found in natural soil, laccase, horseradish peroxidase, and lignin peroxidase. Incubation with the enzymes led to significant reduction in the fraction of SDM extractable from soil, indicating the formation of bound residues. Such transformation was enhanced when the organic matter content in soil is increased or when certain chemical mediators were used along with laccase. The study provided a basis for understanding the environmental fate of sulfonamides and help with the development of remediation methods to mitigate the release of sulfonamides from soil to water.

The aim of the present study was to investigate the persistence of endocrine effects by prochloraz, a fungicide known to have multiple effects on the endocrine system of vertebrates. Since discontinuous exposure is particularly relevant in aquatic ecosystems, an exposure scenario with an exposure phase and a subsequent recovery period was chosen to assess the potential for reversibility of effects by prochloraz on the sexual development of zebrafish (Danio rerio). Zebrafish were exposed to different concentrations of prochloraz (10–300 μg/L) until 60 days post hatch (dph), which includes the period of sexual differentiation. For the subsequent 40 days, fish were either held in clean water for depuration or under further continuous exposure. Histological investigations of the gonads revealed persistent effects on sexual differentiation. The sex ratio was skewed towards males and significantly more intersex individuals were found after exposure to prochloraz at 60 dph. No intersex fish, but masculinized sex ratios were still present after the depuration period, documenting that prochloraz irreversibly affects the sexual development of zebrafish.

Monitoring of cyanobacteria and their associated toxins has intensified in raw water sources of drinking water treatment plants (WTPs) in most countries of the world. However, it is not explored yet for Egyptian WTPs. Therefore, this study was undertaken to investigate the occurrence of cyanobacteria and their microcystin (MC) toxins in the Nile River source water of Damietta WTP during warm months (April–September 2013) and to evaluate the removal efficiency of both cyanobacterial cells and MCs by conventional methods used in this plant as a representative of Egyptian drinking WTPs. The results showed that the source water at the intake of Damietta WTP contained dense cyanobacterial population (1.1–6.6 × 107 cells L⁻¹) dominated by Microcystis aeruginosa. This bloom was found to produce MC-RR and MC-LR. Both cyanobacterial cell density and intracellular MCs in the intake source water increased with the increase in temperature and nutrients during the study period, with maximum values obtained in August. During treatment processes, cyanobacterial cells were incompletely removed by coagulation/flocculation/sedimentation (C/F/S; 91–96.8 %) or sand filtration (93.3–98.9 %). Coagulation/flocculation induced the release of MCs into the ambient water, and the toxins were not completely removed or degraded during further treatment stages (filtration and chlorination). MCs in outflow tank water were detected in high concentrations (1.1–3.6 μg L − 1), exceeding WHO provisional guideline value of 1 μg L − 1 for MC-LR in drinking water. Based on this study, regular monitoring of cyanobacteria and their cyanotoxins in the intake source water and at different stages at all WTPs is necessary to provide safe drinking water to consumers or to prevent exposure of consumers to hazardous cyanobacterial metabolites.

Handling of two nitro-aromatic compounds, 4-nitroaniline (4NA) and 4-nitrophenol (4NP), simultaneously by Chlorella pyrenoidosa was investigated. Algae would secrete or degrade nitro-aromatic compounds depending on different environmental conditions, in which the mode of handling was determined by the relative formation and degradation rate of the compound. Repeated intermittent trigger with externally added 4NA would induce the continuous secretion of 4NA by algae. Simultaneous exposure of both 4NA and 4NP to algae at normal condition would induce the algae to secrete both compounds. An increase in 4NA exposure concentration would elevate both 4NA and 4NP secretion, and that would be inhibited by the stress conditions of starving or lack of oxygen. Increased 4NA degradation per production rate induced by starving or lack of oxygen might explain the subsequent decrease in 4NA secretion in the presence of 4NP in algae. For 4NP in the presence of 4NA, secretion at normal condition was completely stopped and turned to degradation mode in stress conditions. The decreased formation and increased degradation of 4NP during starving for replenishing energy would explain the net degradation of 4NP in starving condition. The condition of lack of oxygen would inhibit the 4NP formation from 4NA via oxidative deamination, while the degradation of 4NP might not be significantly affected because alternative pathway of degradation via nitro-reduction was available. It may lead to the degradation rate exceeding the formation and explain the net degradation of 4NP in the condition of lack of oxygen.

This study investigates the use of calcined eggshells and microalgae for the removal of heavy metals from acid mine drainage (AMD) and the simultaneous enhancement of biomass productivity. The experiment was conducted over a period of 6 days in a hybrid system containing calcined eggshells and the microalgae Chlorella vulgaris. The results show that the biomass productivity increased to ~8.04 times its initial concentration of 0.367 g/L as measured by an optical panel photobioreactor (OPPBR) and had a light transmittance of 95 % at a depth of 305 mm. On the other hand, the simultaneous percent removal of Fe, Cu, Zn, Mn, As, and Cd from the AMD effluent was found to be 99.47 to 100 %. These results indicate that the hybrid system with calcined eggshells and microalgae was highly effective for heavy metal removal in the AMD.

The size distribution of total and water-soluble elemental concentrations in six particle sizes <0.49, 0.49–0.97, 0.97–1.5, 1.5–3.0, 3.0–7.2, and 7.2–30 μm was investigated in Thessaloniki area, N. Greece, at two sites representing urban-traffic and urban-background character during the cold and warm period. The elements As, Cd, Cr, Cu, Pb, Ni, Zn, Ru, and Ir exhibited their highest mass portion in the fine particle mode (0.97–1.5 μm), whereas Al, Ba, Ca, Fe, and Mn occurred predominately in the coarse particle mode (3.0–7.2 μm). The water-soluble elemental fractions exhibited significant spatiotemporal variations and particle size dependence. Possible non-carcinogenic and carcinogenic risks associated with inhalation of particle-bound elements based on total and water-soluble concentrations were in acceptable levels. However, the cumulative risk for all potential particle-bound constituents has to be considered.

Elevated levels of adsorbable organic bromine compounds (AOBr) have been detected in German lakes, and cyanobacteria like Microcystis, which are known for the synthesis of microcystins, are one of the main producers of natural organobromines. However, very little is known about how environmental realistic concentrations of organobromines impact invertebrates. Here, the nematode Caenorhabditis elegans was exposed to AOBr-containing surface water samples and to a Microcystis aeruginosa-enriched batch culture (MC-BA) and compared to single organobromines and microcystin-LR exposures. Stimulatory effects were observed in certain life trait variables, which were particularly pronounced in nematodes exposed to MC-BA. A whole genome DNA-microarray revealed that MC-BA led to the differential expression of more than 2000 genes, many of which are known to be involved in metabolic, neurologic, and morphologic processes. Moreover, the upregulation of cyp- and the downregulation of abu-genes suggested the presence of chronic stress. However, the nematodes were not marked by negative phenotypic responses. The observed difference in MC-BA and microcystin-LR (which impacted lifespan, growth, and reproduction) exposed nematodes was hypothesized to be likely due to other compounds within the batch culture. Most likely, the exposure to low concentrations of organobromines appears to buffer the effects of toxic substances, like microcystin-LR.