International audience | Neonicotinoids are modern insecticides widely used in agriculture worldwide. Their impact on target (nervous system) and non-target (midgut) tissues has been well studied in beneficial insects including honeybees under controlled conditions. However, their detailed effects on pest insects on the field are missing to date. Here, we have studied the effects of the neonicotinoid imidacloprid on the midgut of the pest insect Locusta migratoria caught in the field. We found that in the midgut of imidacloprid-exposed locusts the activity of enzymes involved in reactive oxygen metabolism was perturbed. By contrast, the activity of P450 enzymes that have been shown to be activated in a detoxification response and that were also reported to produce reactive oxygen species was elevated. Probably as a consequence, markers of oxidative stress including protein carbonylation and lipid peroxidation accumulated in midgut samples of these locusts. Histological analyses revealed that their midgut epithelium is disorganized and that the brush border of the epithelial cells is markedly reduced. Indeed, microvilli are significantly shorter, misshapen and possibly non-functional in imidacloprid-treated locusts. We hypothesize that imidacloprid induces oxidative stress in the locust midgut, thereby changing the shape of midgut epithelial cells and probably in turn compromising their physiological function. Presumably, these effects reduce the survival rate of imidacloprid-treated locusts and the damage they cause in the field.

Neonicotinoids are modern insecticides widely used in agriculture worldwide. Their impact on target (nervous system) and non-target (midgut) tissues has been well studied in beneficial insects including honeybees under controlled conditions. However, their detailed effects on pest insects on the field are missing to date. Here, we have studied the effects of the neonicotinoid imidacloprid on the midgut of the pest insect Locusta migratoria caught in the field. We found that in the midgut of imidacloprid-exposed locusts the activity of enzymes involved in reactive oxygen metabolism was perturbed. By contrast, the activity of P450 enzymes that have been shown to be activated in a detoxification response and that were also reported to produce reactive oxygen species was elevated. Probably as a consequence, markers of oxidative stress including protein carbonylation and lipid peroxidation accumulated in midgut samples of these locusts. Histological analyses revealed that their midgut epithelium is disorganized and that the brush border of the epithelial cells is markedly reduced. Indeed, microvilli are significantly shorter, misshapen and possibly non-functional in imidacloprid-treated locusts. We hypothesize that imidacloprid induces oxidative stress in the locust midgut, thereby changing the shape of midgut epithelial cells and probably in turn compromising their physiological function. Presumably, these effects reduce the survival rate of imidacloprid-treated locusts and the damage they cause in the field.

International audience | Sustainable solutions aiming at limiting Reynoutria japonica invasion consist of frequent removal of its aerial biomass. The aims of this study were to measure the accumulation of metallic trace elements (MTE) in R. japonica, and to assess the ecotoxicological risk related to the valorization of the produced biomass. R. japonica fragmented rhizomes were regenerated in pots for 41 days on a control soil (CTL) or a moderately MTE-contaminated soil (POL, 3.6 mg Cd kg −1 DM). Growth traits were recorded, as well as MTE bioconcentration (BCF) and translocation factors (TF) from soil to plant organs. Whatever the MTE and plant organs, BCF remained below one (mean Cd-BCF for stem and leaf: 0.07 and 0.29 for CTL and POL, respectively), conversely to TF (until 2.2 for Cd and Ni in POL soil). When grown on the POL soil, R. japonica stem and leaf Cd content was close to the EU maximum regulatory limit for organic amendments or animal feed. Model simulations suggested that liver and kidney Cd concentrations would exceed the regulatory limit in food when adult cattle or sheep constantly ingest R. japonica grown on the POL soil over 200 to 800 days. The results of the present study will be useful to help managers in selecting efficient and safe solutions for the control of R. japonica invasion.

Sustainable solutions aiming at limiting Reynoutria japonica invasion consist of frequent removal of its aerial biomass. The aims of this study were to measure the accumulation of metallic trace elements (MTE) in R. japonica, and to assess the eco-toxicological risk related to the valorization of the produced biomass. R. japonica fragmented rhizomes were regenerated in pots for 41 days on a control soil (CTL) or a moderately MTE-contaminated soil (POL, 3.6 mg Cd kg⁻¹ DM). Growth traits were recorded, as well as MTE bioconcentration (BCF) and translocation factors (TF) from soil to plant organs. Whatever the MTE and plant organs, BCF remained below one (mean Cd-BCF for stem and leaf: 0.07 and 0.29 for CTL and POL, respectively), conversely to TF (until 2.2 for Cd and Ni in POL soil). When grown on the POL soil, R. japonica stem and leaf Cd content was close to the EU maximum regulatory limit for organic amendments or animal feed. Model simulations suggested that liver and kidney Cd concentrations would exceed the regulatory limit in food when adult cattle or sheep constantly ingest R. japonica grown on the POL soil over 200 to 800 days. The results of the present study will be useful to help managers in selecting efficient and safe solutions for the control of R. japonica invasion.

International audience | Once previous industrial activity has ceased, brownfields are found in urban and suburban environments and managed in different ways ranging from being left untouched to total reconversion. These situations apply to large surface areas often impacted by residual diffuse pollution. Though significant and preventing any sensitive use, residual contamination does not necessarily require treatment. Moreover, conventional treatments show their technical and economic limits in these situations and gentle remediation options such as phytomanagement might appear more relevant to the management of those sites. Thus, these sites face up two major issues: managing moderate contamination levels and providing an alternative use of economic interest. This work proposes to assess a management strategy associating the phytoremediation of organic pollution along with the production of biomass for energy generation production. A 16-week controlled growth experiment was conducted on a soil substrate moderately impacted by multiple pollution (trace elements, mainly Zn and Pb, and hydrocarbons), by associating rhizodegradation with Medicago sativa or biomass production with Robinia pseudoacacia or Alnus incana in monocultures. The effect of a microbial inoculum amendment on the performances of these treatments was also evaluated. Results showed total hydrocarbons (TH), and to a lesser extent polycyclic aromatic hydrocarbons (PAH), concentrations decreased over time, whatever the plant cover. Good biomass production yields were achieved for both tree species in comparison with the control sample, even though R. pseudoacacia seemed to perform better. Furthermore, the quality of the biomass produced was in conformity with the thresholds set by the legislation concerning its use as a renewable energy source.

Once previous industrial activity has ceased, brownfields are found in urban and suburban environments and managed in different ways ranging from being left untouched to total reconversion. These situations apply to large surface areas often impacted by residual diffuse pollution. Though significant and preventing any sensitive use, residual contamination does not necessarily require treatment. Moreover, conventional treatments show their technical and economic limits in these situations and gentle remediation options such as phytomanagement might appear more relevant to the management of those sites. Thus, these sites face up two major issues: managing moderate contamination levels and providing an alternative use of economic interest. This work proposes to assess a management strategy associating the phytoremediation of organic pollution along with the production of biomass for energy generation production. A 16-week controlled growth experiment was conducted on a soil substrate moderately impacted by multiple pollution (trace elements, mainly Zn and Pb, and hydrocarbons), by associating rhizodegradation with Medicago sativa or biomass production with Robinia pseudoacacia or Alnus incana in monocultures. The effect of a microbial inoculum amendment on the performances of these treatments was also evaluated. Results showed total hydrocarbons (TH), and to a lesser extent polycyclic aromatic hydrocarbons (PAH), concentrations decreased over time, whatever the plant cover. Good biomass production yields were achieved for both tree species in comparison with the control sample, even though R. pseudoacacia seemed to perform better. Furthermore, the quality of the biomass produced was in conformity with the thresholds set by the legislation concerning its use as a renewable energy source.

In fish, the gut microbiome plays a crucial role in homeostasis and health and is affected by several organic and inorganic environmental contaminants. Amphidromous fish are sentinel species, particularly exposed to these stressors. We used whole metagenome sequencing to characterize the gut microbiome of wild European eels (Anguilla anguilla) at a juvenile stage captured from three sites with contrasted pollution levels in term of heavy metals and persistent organic pollutants. The objectives were to identify what parameters could alter the gut microbiome of this catadromous fish and to explore the potential use of microbiota as bioindicators of environment quality. We identified a total of 1079 microbial genera. Overall, gut microbiome was dominated by Proteobacteria, Firmicutes and Actinobacteria. Alpha and beta diversity were different amongst sites and could be explained by a reduced number of environmental and biological factors, specifically the relative abundance of fish preys in eels’ diet, PCB101, γHCH (lindane), transnonachlor and arsenic. Furthermore, we identified a series of indicator taxa with differential abundance between the three sites. Changes in the microbial communities in the gut caused by environmental pollutants were previously undocumented in European eels. Our results indicate that microbiota might represent another route by which pollutants affect the health of these aquatic sentinel organisms.

In fish, the gut microbiome plays a crucial role in homeostasis and health and is affected by several organic and inorganic environmental contaminants. Amphidromous fish are sentinel species, particularly exposed to these stressors. We used whole metagenome sequencing to characterize the gut microbiome of wild European eels (Anguilla anguilla) at a juvenile stage captured from three sites with contrasted pollution levels in term of heavy metals and persistent organic pollutants. The objectives were to identify what parameters could alter the gut microbiome of this catadromous fish and to explore the potential use of microbiota as bioindicators of environment quality. We identified a total of 1079 microbial genera. Overall, gut microbiome was dominated by Proteobacteria, Firmicutes and Actinobacteria. Alpha and beta diversity were different amongst sites and could be explained by a reduced number of environmental and biological factors, specifically the relative abundance of fish preys in eels’ diet, PCB101, γHCH (lindane), transnonachlor and arsenic. Furthermore, we identified a series of indicator taxa with differential abundance between the three sites. Changes in the microbial communities in the gut caused by environmental pollutants were previously undocumented in European eels. Our results indicate that microbiota might represent another route by which pollutants affect the health of these aquatic sentinel organisms.

International audience | In fish, the gut microbiome plays a crucial role in homeostasis and health and is affected by several organic and inorganic environmental contaminants. Amphidromous fish are sentinel species, particularly exposed to these stressors. We used whole metagenome sequencing to characterize the gut microbiome of wild European eels (Anguilla anguilla) at a juvenile stage captured from three sites with contrasted pollution levels in term of heavy metals and persistent organic pollutants. The objectives were to identify what parameters could alter the gut microbiome of this catadromous fish and to explore the potential use of microbiota as bioindicators of environment quality. We identified a total of 1079 microbial genera. Overall, gut microbiome was dominated by Proteobacteria, Firmicutes and Actinobacteria. Alpha and beta diversity were different amongst sites and could be explained by a reduced number of environmental and biological factors, specifically the relative abundance of fish preys in eels’ diet, PCB101, γHCH (lindane), transnonachlor and arsenic. Furthermore, we identified a series of indicator taxa with differential abundance between the three sites. Changes in the microbial communities in the gut caused by environmental pollutants were previously undocumented in European eels. Our results indicate that microbiota might represent another route by which pollutants affect the health of these aquatic sentinel organisms.

International audience | Soil trace metal concentrations (e.g., cadmium, copper, lead, zinc) in vegetable gardens have often been observed as exceeding the geochemical background levels. These metals are a threat both to soil and plant functioning and to human health through consumption of contaminated vegetables. We used a mass balance-based model to predict the four metal (Cd, Cu, Pb, Zn) concentrations in soils after a century’s cultivation for 104 urban vegetable gardens, located in three French metropolises, Nancy, Nantes, and Marseille, based on a survey of gardening practices. If current gardening practices are maintained, an increase in soil Cd (35% on average), Cu (183%), and Zn (27%) contents should occur after a century. Soil Pb concentration should not vary consistently. Organic amendments are the major source of Cd, Pb, and Zn, followed by chemical fertilizer while fungicide application is the major source of Cu. Cessation of chemical fertilizer use would only slightly reduce the accumulation of the four metals. The solubility of the four metals would decrease significantly after a century, when pH increases by one unit. A liming practice of acidic soils should therefore be a feasible way to prevent any increase in the metal mobility and bioavailability.

Soil trace metal concentrations (e.g., cadmium, copper, lead, zinc) in vegetable gardens have often been observed as exceeding the geochemical background levels. These metals are a threat both to soil and plant functioning and to human health through consumption of contaminated vegetables. We used a mass balance-based model to predict the four metal (Cd, Cu, Pb, Zn) concentrations in soils after a century’s cultivation for 104 urban vegetable gardens, located in three French metropolises, Nancy, Nantes, and Marseille, based on a survey of gardening practices. If current gardening practices are maintained, an increase in soil Cd (35% on average), Cu (183%), and Zn (27%) contents should occur after a century. Soil Pb concentration should not vary consistently. Organic amendments are the major source of Cd, Pb, and Zn, followed by chemical fertilizer while fungicide application is the major source of Cu. Cessation of chemical fertilizer use would only slightly reduce the accumulation of the four metals. The solubility of the four metals would decrease significantly after a century, when pH increases by one unit. A liming practice of acidic soils should therefore be a feasible way to prevent any increase in the metal mobility and bioavailability.