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Response of Solanum lycopersicum L. to diclofenac – Impacts on the plant’s antioxidant mechanisms
2020
Sousa, Bruno | Lopes, Jorge | Leal, André | Martins, Maria | Soares, Cristiano | Valente, Inês M. | Rodrigues, José A. | Fidalgo, Fernanda | Teixeira, Jorge
One emerging problem that recently has become a vastly acknowledged topic of concern is the environmental contamination by pharmaceuticals. Diclofenac (DCF) is one of the most common pharmaceuticals found, due to its high utilization and low removal rate in wastewater treatment processes. In this work, Solanum lycopersicum L. was used as a model to unravel how DCF contamination can affect crops, focusing on the internal mechanisms triggered by this exposure. For this purpose, plants were exposed to two different DCF concentrations (0.5 mg L⁻¹ and 5 mg L⁻¹). Results obtained here point towards a loss of shoot performance when plants were exposed to very high concentrations of DCF, but no delay or loss of yield in the flowering and fruit stages were ascribed to DCF contamination. Our data shows that a state of oxidative stress due to high reactive oxygen species accumulation was associated with this contamination, with very high DCF levels leading to a rise of lipid peroxidation, possibly accentuated by the inhibition of ROS-scavenging enzymes and unable to be counteracted by the visible upregulation of proline and the thiol-based redox network. Overall, these results allow to infer that in the current environmental context, no noticeable negative effects should be associated with the presence of DCF in soils where this crop is cultivated. However, the oxidative stress and lower biomass associated with the highest concentration are alarming, since DCF levels in the environment are continuously increasing and further measures are necessary to assess this problematic.
Afficher plus [+] Moins [-]Dermal bioaccessibility and absorption of polycyclic aromatic hydrocarbons (PAHs) in indoor dust and its implication in risk assessment
2020
Luo, Kesong | Zeng, Diya | Kang, Yuan | Lin, Xunyang | Sun, Na | Li, Cheng | Zhu, Mengqi | Chen, Zhenwen | Man, Yu Bon | Li, Hui
Numerous studies have focused on assessing the risk of human exposure to polycyclic aromatic hydrocarbons (PAHs) in indoor dust via dermal contact. However, the dermal bioaccessibility and dermal absorption of PAHs in indoor dust have seldom been reported. In the present study, the effects of temperature, sweat ratio, solid-liquid ratio and incubation time on the dermal bioaccessibility of PAHs were examined. Naphthalene, phenanthrene, pyrene and benzo[a]pyrenewere selected for examination in an absorption assay with keratinocyte cells. The results showed the release of PAHs from indoor dust fitted a first-order one-compartment model. Naphthalene had the highest rate of release, which was consistent with the bioaccessibility assay results. In addition, the absorption rate of naphthalene and phenanthrene by keratinocytes was higher than that of pyrene and benzo[a]pyrene, with the latter being of higher molecular weight. These results indicated that low molecular weight PAHs were much more easily absorbed via dermal contact than were high molecular weight PAHs. The dermal bioavailability of PAHs in indoor dust was estimated by multiplying the bioaccessibility of PAHs in indoor dust by the ratio of dermal absorption by skin cells, and ranged from 0.12 to 51.0%. These data will be useful in risk assessments.
Afficher plus [+] Moins [-]Microplastic particles increase arsenic toxicity to rice seedlings
2020
Dong, Youming | Gao, Minling | Song, Zhengguo | Qiu, Weiwen
Hydroponic experiments were conducted to study the effects of microplastic particles of polystyrene (PS) and polytetrafluoroethylene (PTFE) on arsenic (As) content in leaves and roots of rice seedlings, and the changes in root vigor and physiological and biochemical indicators under single or combined PS and PTFE with As(III) treatment. Rice biomass decreased with increasing concentrations of PS, PTFE, and As(III) in the growth medium. The highest root (leaf) biomass decreases were 21.4% (10.2%), 25.4% (11.8%), and 26.2% (16.2%) with the addition of 0.2 g L⁻¹ PS, 0.2 g L⁻¹ PTFE, and 4 mg L⁻¹ As(III), respectively. Microplastic particles and As(III) inhibited biomass accumulation by inhibiting root activity and RuBisCO activity, respectively. The addition of As(III) and microplastic particles (PS or PTFE) inhibited photosynthesis through non-stomatal and stomatal factors, respectively; furthermore, net photosynthetic rate, chlorophyll fluorescence, and the Chl a content of rice were reduced with the addition of As(III) and microplastic particles (PS or PTFE). Microplastic particles and As(III) induced an oxidative burst in rice tissues through mechanical damage and destruction of the tertiary structure of antioxidant enzymes, respectively, thereby increasing O₂⁻ and H₂O₂ in roots and leaves, inducing lipid peroxidation, and destroying cell membranes. When PS and PTFE were added at 0.04 and 0.1 g L⁻¹, respectively, the negative effects of As(III) on rice were reduced. Treatment with 0.2 g L⁻¹ PS or PTFE, combined with As(III), had a higher impact on rice than the application of As(III) alone. PS and PTFE reduced As(III) uptake, and absorbed As decreased with the increasing concentration of microparticles. The underlying mechanisms for these effects may involve direct adsorption of As, competition between As and microplastic particles for adsorption sites on the root surface, and inhibition of root activity by microplastic particles.
Afficher plus [+] Moins [-]Digestive solubilization of Cd in highly-contaminated sediment by marine deposit feeders: The roles of intestinal surfactants in Cd mobilization and Re-Adsorption processes
2020
Wu, Xing | Klerks, Paul L. | Bi, Ran | Liu, Wenhua | Yuan, Zi-Dan | Ma, Xu | Zhang, Guo-Qing | Wang, Shao-Feng | Jia, Yong-Feng
Marine deposit feeders are of ecological significance in transferring sedimentary Cd along aquatic food chains. A key process for this transfer is these organisms’ dietary uptake of Cd via solubilization of Cd present in ingested contaminated sediment. To better understand the bioavailability of sedimentary Cd to deposit feeders, the present study used in vitro extraction experiments to explore the contribution of different digestive agents (proteins, amino acids and surfactants) to the solubilization of Cd from sediment collected in a highly-contaminated Chinese bay. This was done for various commercially-available mimetic digestive agents (the protein BSA, a mixture of amino acids, and the surfactants rhamnolipid and SDS), and for proteins and surfactants collected from the gut juice of a sipunculan worm. The Cd mobilization capacity of BSA was significantly higher than that of the amino acids and the commercial surfactants. In the presence of BSA, > 70% of the released Cd became associated with this protein. In contrast, the digestive proteins from the sipunculan had a lower Cd mobilization capacity than was the case for the other digestive agents and the majority of the released Cd (∼80%) was associated with small molecular weight fractions. The differences in Cd mobilization between the BSA and the digestive proteins were attributed to differences in their sediment-adsorption tendencies and their Cd-complexing capacities. While the digestive surfactants had minor effects on the release of sedimentary Cd, they significantly enhanced Cd mobilization by the digestive proteins when both were present simultaneously. Our results suggest that the characteristics of proteins should be considered when using commercially-available mimetic digestive agents to explore Cd bioavailability in sediments. Furthermore, digestive surfactants seem to have important effects on the solubilization of Cd during gut passage by reducing the adsorption of the digestive proteins to the sediments.
Afficher plus [+] Moins [-]Polybrominated diphenyl ethers and alternative halogenated flame retardants in mangrove plants from Futian National Nature Reserve of Shenzhen City, South China
2020
Hu, Yongxia | Sun, Yuxin | Pei, Nancai | Zhang, Zaiwang | Li, Huawei | Wang, Weiwei | Xie, Jinli | Xu, Xiangrong | Luo, Xiaojun | Mai, Bixian
Halogenated flame retardants (HFRs) are ubiquitous in the environment, but little information is available about the bioaccumulation of HFRs in mangrove plants. In this study, three mangrove plant species were collected from Futian National Nature Reserve of Shenzhen City, South China to investigate the bioaccumulation of polybrominated diphenyl ethers (PBDEs) and several alternative halogenated flame retardants (AHFRs), including decabromodiphenyl ethane (DBDPE), 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE), hexabromobenzene (HBB), pentabromotoluene (PBT), tetrabromop-xylene (pTBX), pentabromoethylbenzene (PBEB) and dechlorane plus (DP). The mean concentrations of PBDEs, DBDPE, BTBPE, pTBX, PBT, PBEB, HBB and DP in mangrove plant species were 2010, 1870, 36.2, 18.7, 40.1, 17.8, 9.68 and 120 pg g⁻¹ dry weight, respectively. PBDEs were the dominant HFRs in mangrove plant tissues, followed by DBDPE. The relative abundance of BDE 209 in three mangrove plant tissues were much lower than those in sediments. Significant negative relationships between log root bioaccumulation factors and log Kₒw, and between log TFᵣ₋ₛ (from root to stem) and log Kₒw were observed, indicating that HFRs with low hydrophobicity were easily absorbed by mangrove roots and stems. A positive correlation between log TFₛ₋ₗ (from stem to leaf) and log Kₒw were found, suggesting that air-leaf exchange may occur in mangrove plants. This study highlights the uptake of HFRs by mangrove plants, which can be used as remediation for HFRs contamination in the environment.
Afficher plus [+] Moins [-]Dust and bullets: Stable isotopes and GPS tracking disentangle lead sources for a large avian scavenger
2020
Lead intoxication is an important threat to human health and a large number of wildlife species. Animals are exposed to several sources of lead highlighting hunting ammunition and lead that is bioavailable in topsoil. Disentangling the role of each in lead exposure is an important conservation issue, particularly for species potentially affected by lead poisoning, such as vultures. The identification of lead sources in vultures and other species has been classically addressed by means of stable-isotope comparisons, but the extremely varied isotope signatures found in ammunition hinders this identification when it overlaps with topsoil signatures. In addition, assumptions related to the exposure of individual vultures to lead sources have been made without knowledge of the actual feeding grounds exploited by the birds. Here, we combine lead concentration analysis in blood, novel stable isotope approaches to assign the origin of the lead and GPS tracking data to investigate the main foraging grounds of two Iberian griffon vulture populations (N = 58) whose foraging ranges differ in terms of topsoil lead concentration and intensity of big game hunting activity. We found that the lead signature in vultures was closer to topsoil than to ammunition, but this similarity decreased significantly in the area with higher big game hunting activity. In addition, attending to the individual home ranges of the tracked birds, models accounting for the intensity of hunting activity better explained the higher blood lead concentration in vultures than topsoil exposure. In spite of that, our finding also show that lead exposure from topsoil is more important than previously thought.
Afficher plus [+] Moins [-]Selenium treatment modulates fluoride distribution and mitigates fluoride stress in tea plant (Camellia sinensis (L.) O. Kuntze)
2020
Niu, Huiliang | Zhan, Kui | Xu, Wei | Peng, Chuanyi | Hou, Chaoyuan | Li, Yeyun | Hou, Ruyan | Wan, Xiaochun | Cai, Huimei
Tea plants (Camellia sinensis (L.) O. Kuntze) can hyperaccumulate fluoride (F). The accumulation of F in tea leaves may induce serious health problems in tea consumers. It has been reported that selenium (Se) could reduce the accumulation of heavy metals in plants. Thus, the aim of this study was to investigate whether exogenous Se could reduce F accumulation in tea plant. The results showed that Se treatment could decrease F content in tea leaves, increase F accumulation in roots, decrease the proportion of water-soluble F in tea leaves and increase the Se content. Low F levels promoted the accumulation of Se in tea plants. Se treatment could modulate F-induced oxidative injury by decreasing malondialdehyde level and increasing the activities of superoxide dismutase, peroxidase and catalase. Moreover, Se inhibited F-induced increase in leaf iron, calcium, aluminum, leaf and root magnesium and lead contents. These results showed that Se application could decrease F content and increase Se content in tea leaves, which may be served as a novel strategy for production of healthy tea.
Afficher plus [+] Moins [-]Numerical analysis and modeling of two-loop experimental setup for measurements of radon diffusion rate through building and insulation materials
2020
Szajerski, Piotr | Zimny, Arkadiusz
Radon is a natural radioactive gas present in the environment, which is considered as the second most important lung cancer cause worldwide. Currently, radon gas is under focus and was classified as contaminant of emerging concern, which is responsible for serious biological/health effects in human. In presented work we propose the numerical model and analysis method for radon diffusion rate measurements and radon transport parameters determination. The experimental setup for radon diffusion was built in a classical, two chamber configuration, in which the radon source and outlet reservoirs are separated by the sample being tested. The main difference with previously known systems is utilization of only one radon detector, what was achieved by a careful characterization of the Rn-222 source and development of a numerical model, which allows for exact determination of radon transport parameters by fitting simulated radon concentration profile in the outlet reservoir to experimental data. For verification of the developed system, several insulation materials commonly used in building industry and civil engineering, as well as, common building materials (gypsum, hardened cement paste, concrete) were tested for radon diffusion rate through these barriers. The results of radon transmittance, permeability and diffusion coefficients for investigated materials are in compliance with values known previously from the literature. The analysis method is fast and efficient, and requires measurement period varying from a dozen or so hours up to 2–3 days depending on material properties. The described method is entirely based on a numerical analysis of the proposed differential equation model using freely available SCILAB software and experimental data obtained during sample measurements.
Afficher plus [+] Moins [-]High-content screening in zebrafish identifies perfluorooctanesulfonamide as a potent developmental toxicant
2020
Dasgupta, Subham | Reddam, Aalekhya | Liu, Zekun | Liu, Jinyong | Volz, David C.
Per- and polyfluoroalkyl substances (PFASs) have been used for decades within industrial processes and consumer products, resulting in frequent detection within the environment. Using zebrafish embryos, we screened 38 PFASs for developmental toxicity and revealed that perfluorooctanesulfonamide (PFOSA) was the most potent developmental toxicant, resulting in elevated mortality and developmental abnormalities following exposure from 6 to 24 h post fertilization (hpf) and 6 to 72 hpf. PFOSA resulted in a concentration-dependent increase in mortality and abnormalities, with surviving embryos exhibiting a >12-h delay in development at 24 hpf. Exposures initiated at 0.75 hpf also resulted in a concentration-dependent delay in epiboly, although these effects were not driven by a specific sensitive window of development. We relied on mRNA-sequencing to identify the potential association of PFOSA-induced developmental delays with impacts on the embryonic transcriptome. Relative to stage-matched vehicle controls, these data revealed that pathways related to hepatotoxicity and lipid transport were disrupted in embryos exposed to PFOSA from 0.75 to 14 hpf and 0.75 to 24 hpf. Therefore, we measured liver area as well as neutral lipids in 128-hpf embryos exposed to vehicle (0.1% DMSO) or PFOSA from 0.75 to 24 hpf and clean water from 24 to 128 hpf, and showed that PFOSA exposure from 0.75 to 24 hpf resulted in a decrease in liver area and increase in yolk sac neutral lipids at 128 hpf. Overall, our findings show that early exposure to PFOSA adversely impacts embryogenesis, an effect that may lead to altered lipid transport and liver development.
Afficher plus [+] Moins [-]Human health risk assessment for exposure to BTEXN in an urban aquifer using deterministic and probabilistic methods: A case study of Chennai city, India
2020
Rajasekhar, Bokam | Nambi, Indumathi M. | Govindarajan, Suresh Kumar
The aquifer in Tondiarpet, Chennai, had been severely contaminated with petroleum fuels due to an underground pipeline leakage. Groundwater samples were analyzed quarterly for priority pollutants such as benzene, toluene, ethylbenzene, xylenes, and naphthalene (BTEXN) using purge and trap gas chromatography and mass spectrometer from 2016 to 2018. The maximum concentrations of BTEXN in groundwater at the site were found to be greater than the permissible limits significantly. Among the five sampling locations (MW1, MW2, MW3, MW4, and MW5), mean BTEXN levels were found to be higher near MW2, confirming the source location of petroleum leakage. Human health risk assessment was carried out using deterministic and probabilistic methods for exposure to BTEXN by oral and dermal exposure pathways. Risk analysis indicated that mean cancer and non-cancer risks were many times higher than the allowable limits of 1E-06 and 1 respectively in all age groups (children, teens, and adults), implying the adverse health effects. Oral exposure is predominately contributing (60–80%) to the total health risk in comparison to the dermal exposure route. Variability and uncertainty were addressed using the Monte Carlo simulations and the resultant minimum, maximum, 5th, 95th, and mean percentile risks were predicted. Under the random exposure conditions to BTEXN, it was estimated that the risk would become unacceptable for >98.7% of the exposed population. Based on the sensitivity analysis, exposure duration, and ingestion rate are the crucial variables contributing significantly to the health risk. As part of the risk management, preliminary remediation goals for the study site were estimated, which require >99% removal of the BTEXN contamination for risk-free exposures. It is suggested that the residents of Tondiarpet shouldn’t utilize the contaminated groundwater mainly for oral ingestion to lower the cancer incidence related to exposure to BTEXN.
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