We assessed dietary exposure of the little owl Athene noctua to trace metal contamination in a Dutch Rhine River floodplain area. Diet composition was calculated per month for three habitat types, based on the population densities of six prey types (earthworms, ground beetles and four small mammal species) combined with the little owl’s functional response to these prey types. Exposure levels showed a strong positive relationship with the dietary fraction of earthworms, but also depended on the dietary fraction of common voles, with higher common vole fractions resulting in decreasing exposure levels. Spatio-temporal changes in the availability of earthworms and common voles in particular resulted in considerable variation in exposure, with peaks in exposure exceeding a tentative toxicity threshold. These findings imply that wildlife exposure assessments based on a predefined, average diet composition may considerably underestimate local or intermittent peaks in exposure.

One concern about the chelant-enhanced phytoextraction is the potential metal leaching associated with chelant application. A field study was carried out and the metal leaching along the 60-cm depth soil profiles were evaluated within 36 days after the biodegradable chelant EDDS was applied. Results showed EDDS significantly increased soluble Cu in the top 5 cm soil layer 1 day after the application, and the increase of soluble metals was generally limited in the top 20 cm soil. Metal speciation analysis indicated all Cu and Zn were in forms of Cu-EDDS and Zn-EDDS complexes in soil solution, and Ca was the major competitor with trace metals to EDDS. The soluble metals decreased quickly with time, and no significant difference was observed in the extractable Cu between EDDS treatments and the controls 22 days after the EDDS addition. The potential leaching associated with biodegradable EDDS addition may be controlled under field conditions.

We investigated presence and levels of DDT in 163 breast milk samples from four South African villages where, in three of them, malaria is controlled with DDT-sprayed indoors. Mean ΣDDT levels in breast milk were 18, 11, and 9.5 mg/kg mf (milk fat) from the three DDT-sprayed villages, respectively, including the highest ΣDDT level ever reported for breast milk from South Africa (140 mg/kg mf). Understanding the causes for these differences would be informative for exposure reduction intervention. The Provisional Tolerable Daily Intake (PTDI) for DDT by infants, and the Maximum Residue Limit (MRL) were significantly exceeded. DDT had no effect on duration of lactation. There were indications (not significant) from DDT-sprayed villages that first-born female infants drink milk with more ΣDDT than first-born male infants, and vice versa for multipara male and female infants, suggesting gender involvement on levels of DDT in breast milk – requiring further investigation.

This study investigates the column leaching of a soil contaminated mainly with Cr and Ni by using two chelants: citric acid (biodegradable) and EDTA (non-biodegradable) followed with water rinse. The chelants lead to Cr and Ni leaching, in addition to major elements (Ca, Fe, Mg, Al, Mn and Zn) showing the dissolution of soil mineral constituents. EDTA leaches more major elements and Ni than citric acid related to the respective stability of metal–chelant complexes; citric acid leaches more Cr than EDTA, certainly because of a substitution reaction with Cr(VI). In the case of alternating chelant/water applications, leaching occurs during the chelant applications, but also during water applications. In the case of chelant/water applications followed by continuous water application, both Cr and Ni leach over time. This increased mobility could be due to the residual chelant present in soil as well as to the dissolution/mobilization of mineral or organic soil fractions.

Total mercury concentrations are presented for 19 Swedish watercourses 2000–2010, together with an analysis of factors affecting these concentrations in space and time. Organic matter (OM) measured as absorbance at 420nm (Abs₄₂₀) and total organic carbon (TOC) were the variables most strongly correlated with THg concentrations in the pooled dataset from all 19 watercourses, explaining 66% and 61% of the variance respectively. The correlation between THg and OM indicates that OM is the main controlling factor independent of geographical variation in Hg deposition, geology, or any other factor evaluated in this study. Despite an increase in TOC concentrations at most sites during the study period, THg increased in only one of the watercourses, and the THg/TOC ratio decreased significantly at six sites. The Abs₄₂₀ did not increase like TOC. We suggest that OM-fractions absorbing at 420nm are more important for Hg mobilization than other OM-fractions.

Consequences of irrigation by arsenic (As) enriched groundwater were assigned in the Hetao Plain, part of Chinas’ Inner Mongolia Autonomous Region. Examinations followed the As flow path from groundwater to soil and finally plants. A sunflower and a maize field were systematically sampled, each irrigated since three years with saline well water, characterized by elevated As concentrations (154 and 238μgL⁻¹). The annual As input per m² was estimated as 120 and 186mg, respectively. Compared to the geogenic background, As concentrations increased toward the surface with observed enrichments in topsoil being relatively moderate (up to 21.1mgkg⁻¹). Arsenic concentrations in plant parts decreased from roots toward leaves, stems and seeds. It is shown that the bioavailability of As is influenced by a complex interplay of partly counteracting processes. To prevent As enrichment and soil salinization, local farmers were recommended to switch to a less problematic water source.

Next to imposing direct lethal effects, pollutants may also indirectly impose mortality by making prey organisms more vulnerable to predation. We report that four water boatmen species differed strongly in direct endosulfan-imposed mortality, and only the species that suffered highest mortality, Sigara iactans, also showed a reduction in escape swimming speed. While head AChE activity was inhibited in all four species, body ChE was only inhibited in S. iactans where it covaried with escape swimming speed, indicating a mechanistic link between body ChE and swimming speed. Our study underscores the need for risk assessment to consider sublethal pollutant effects, which may considerably affect survival rates under natural conditions, also when testing concentrations of a pesticide that cause direct mortality. Such sublethal effects may generate discrepancies between laboratory and field studies and should be considered when designing safety factors for toxicants where the risk assessment is solely based on LC50 values.

Phragmites australis shows potential for the phytoremediation of chlorinated chemicals. Also there has been some attempt to determine the phytotoxic effects of organochlorines (OC). This study reports for lindane (HCH), monochlorobenzene (MCB), 1,4-dichlorobenzene (DCB) and 1,2,4-trichlorobenzene (TCB), a no-observed-effect-concentration (NOEC₇d) that was 1000–300,000 times higher than environmental concentrations. Nevertheless, the combined OC mixture (NOEC₇d level of each congener) induced a synergistic toxic effect, causing a severe drop (70%) in chlorophyll concentration. The mixture 0.2mgL⁻¹ MCB+0.2mgL⁻¹ DCB+2.5mgL⁻¹ TCB+0.175mgL⁻¹ HCH, that was 15 times more concentrated than environmental OC mixture, did not cause phytotoxicity during 21 days. Antioxidant enzymes were affected immediately after the start of exposure (3 days), but the plants showed no signs of stress thereafter. These data suggest that environmental OC mixtures do not pose a significant risk to P. australis.

Chemicals are often found in the environment as complex mixtures. There has been a large effort in the last decade to assess the combined effect of chemicals, using the conceptual models of Concentration Addition and Independent Action, but also including synergistic, antagonistic, dose-level and dose–ratio dependent deviations from these models. In the present study, single and mixture toxicity of atrazine, dimethoate, lindane, zinc and cadmium were studied in Folsomia candida, assessing survival and reproduction. Different response patterns were observed for the different endpoints and synergistic patterns were observed when pesticides were present. Compared with the previously tested Enchytraeus albidus and Porcellionides pruinosus, the mixture toxicity pattern for F. candida was species specific. The present study highlights the importance of studying toxicity of chemicals mixtures due to the observed potentiation of effects and confirms that for an adequate ecologically relevant risk assessment different organisms and endpoints should be included.

Low temperatures and frequent soil freeze–thaw in polar environments present challenges for the immobilisation of metals. To address these challenges we investigated the chemical forms of Pb, Zn and Cu in an Antarctic landfill, examined in vitro reaction kinetics of these metals and orthophosphate at 2 and 22 °C for up to 185 days, and subjected the products to freeze–thaw. Reaction products at both temperatures were similar, but the rate of production varied, with Cu-PO₄ phases forming faster, and the Zn- and Pb-PO₄ phases slower at 2 °C. All metal-orthophosphate phases produced were stable during a 2.5 h freeze–thaw cycle to −30 °C. Metal immobilisation using orthophosphate can be successful in polar regions, but treatments will need to consider differing mineral stabilities and reaction rates at low temperatures.