Hydroxylated polychlorobiphenyls (OH-PCBs) are major metabolites of PCBs that are widely distributed in the environment. While the effects of penta- to hepta-chlorinated OH-PCBs on neuronal differentiation have been widely reported, those of lower chlorinated OH-PCBs have not been extensively studied. To investigate the effects of lower chlorinated OH-PCBs on neuronal development, we studied the effects of mono- to hexa-chlorinated OH-PCBs on PC12 cells. Morphological changes were examined using an automatic system IN Cell Analyzer. Seventeen of the 20 OH-PCBs investigated promoted neuronal elongation in an OH-PCB concentration-dependent manner, while three OH-PCB congeners suppressed neuronal elongation based on Dunnett’s analysis. In particular, the top five OH-PCBs (4OH-PCB2, 4′OH-PCB3, 4′OH-PCB25, 4′OH-PCB68, and 4′OH-PCB159), which have hydroxyl groups at the para-position and chlorine substitutions at the 2, 4, or 3′ positions, significantly promoted neuronal elongation. Moreover, these neuronal elongations were suppressed by U0126, and phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was observed in PC12 cells treated with 4OH-PCB2, 4′OH-PCB25, and 4′OH-PCB159. Taken together, our results indicate that the effect of OH-PCB on neuronal development is not dependent on the number of chlorine groups but on the chemical structure, and the mitogen-activated kinase kinase (MEK)-ERK1/2 signaling pathway is involved in this process.

Virus is one of the most potentially harmful microorganisms in groundwater. In this paper, the effects of hydrodynamic and hydrogeochemical conditions on the transportation of the colloidal virus considering managed aquifer recharge were systematically investigated. Escherichia coli phage, vB_EcoM-ep3, has a broad host range and was able to lyse pathogenic Escherichia coli. Bacteriophage with low risk to infect human has been found extensively in the groundwater environment, so it is considered as a representative model of groundwater viruses. Laboratory studies were carried out to analyze the transport of the Escherichia coli phage under varying conditions of pH, ionic strength, cation valence, flow rate, porous media, and phosphate buffer concentration. The results indicated that decreasing the pH will increase the adsorption of Escherichia coli phage. Increasing the ionic strength, either Na⁺ or Ca²⁺, will form negative condition for the migration of Escherichia coli phage. A comparison of different cation valence tests indicated that changes in transport and deposition were more pronounced with divalent Ca²⁺ than monovalent Na⁺. As the flow rate increases, the release of Escherichia coli phage increases and the retention of Escherichia coli phage in the aquifer medium reduces. Changes in porous media had a significant effect on Escherichia coli phage migration. With increase of phosphate buffer concentration, the suspension stability and migration ability of Escherichia coli phage are both increased. Based on laboratory-scale column experiments, a one-dimensional transport model was established to quantitatively describe the virus transport in saturated porous medium.

The risk of water shortage caused by uncertainties, such as frequent drought, varied precipitation, multiple water resources, and different water demands, brings new challenges to the water transfer projects. Uncertainties exist for transferring water and local surface water; therefore, the relationship between them should be thoroughly studied to prevent water shortage. For more effective water management, an uncertainty-based water shortage risk assessment model (UWSRAM) is developed to study the combined effect of multiple water resources and analyze the shortage degree under uncertainty. The UWSRAM combines copula-based Monte Carlo stochastic simulation and the chance-constrained programming-stochastic multiobjective optimization model, using the Lunan water-receiving area in China as an example. Statistical copula functions are employed to estimate the joint probability of available transferring water and local surface water and sampling from the multivariate probability distribution, which are used as inputs for the optimization model. The approach reveals the distribution of water shortage and is able to emphasize the importance of improving and updating transferring water and local surface water management, and examine their combined influence on water shortage risk assessment. The possible available water and shortages can be calculated applying the UWSRAM, also with the corresponding allocation measures under different water availability levels and violating probabilities. The UWSRAM is valuable for mastering the overall multi-water resource and water shortage degree, adapting to the uncertainty surrounding water resources, establishing effective water resource planning policies for managers and achieving sustainable development.

The study examines trends of scattering, absorption and total aerosol optical depths (SAOD, AAOD and AOD) over India and surrounding oceanic regions and explores role of local production, long-range transport and atmospheric dynamics on observed trends. Long-term satellite observations are used to estimate trends and assess their statistical significance. Significant spatial and seasonal changes are observed in trends of SAOD, AAOD and AOD. AOD is observed to be increasing during post monsoon and winter over most of the land mass and surrounding oceanic regions, whereas decreasing trends over land and increasing trends over oceanic regions are observed in pre-monsoon and summer months. In general, SAOD and AAOD show similar trends (if there is any) as that of AOD over most of the regions in most of the months. Strongest positive trends over land regions are observed in November with trend of AOD greater than 0.01 year⁻¹, especially over Indo-Gangetic Plain (IGP). Increase of AOD over IGP in post monsoon is contributed significantly by absorbing aerosols with rate of increase ~ 0.005 AAOD year⁻¹. AAODs are observed to be increasing over Arabian Sea and Bay of Bengal (BoB) in December also, with rate ~ 0.003 AAOD year⁻¹. Strongest positive trends over Arabian Sea and BoB are observed in June with rate of increase greater than 0.02 AOD year⁻¹, whereas strong negative trends are observed over north-west India in the same period with rate of decrease greater than 0.02 AOD year⁻¹. Over IGP, AOD, AAOD and SAOD show contrasting trends in winter and summer seasons. AAOD exhibits strongest decreasing trend over IGP during April–June. Positive trends of AOD over Arabian Sea and BoB are favoured significantly by changes in circulation dynamics. Atmospheric convergence is observed to be strengthening over these regions in April and June, leading to more accumulation and hence positive trends of AOD. Aerosol transport over to the Arabian Sea is observed to be enhancing and contributing significantly to AOD increase over the Arabian Sea in pre-monsoon and summer months. Enhancement in aerosol transport over to the Arabian Sea is observed in pre-monsoon at higher altitudes above 3 km, whereas it is observed in summer at lower levels. However, decreasing trends of AOD over north-west India and IGP during pre-monsoon and summer are observed to be due to decrease in aerosol transport from the continental regions at the west.

Solid-liquid separation (SLS) plays a dominant role in various chemical industries. Nowadays, low efficiency of SLS also become a significant problem in heavy metal (HM) wastewater treatment, affecting the effluent quality (HM concentration and turbidity) and overall process economy. In this context, we summarize here the occurrence of solids in HM wastewater, as well as typical SLS operations used in HM wastewater treatment, including sedimentation, flotation, and centrifugation. More important, this article reviews the improvement of the SLS operations by some technologies, including coagulation, flocculation, ballasted method, seeding method, granular sludge strategy, and external field enhancement. It is noted that abiological granular sludge strategy and magnetic field enhancement often possess higher SLS efficiency (faster settling velocity or shorter separation time) than other methods. Hence, the two strategies stand out as promising tools for improving SLS in HM wastewater treatment, but further research is required regarding scalability, economy, and reliability.

PAH biodegradation in plant rhizosphere has been investigated in many studies, but the timescale of degradation and degrading bacteria activity was rarely considered. We explored the impact of plants on the temporal variability of PAH degradation, microbial abundance, activity, and bacterial community structure in a rhizotron experiment. A historically contaminated soil was spiked with PAHs, planted or not with alfalfa, over 22 days with sampling once a week. In both conditions, most of the spiked PAHs were dissipated during the first week, conducting to polar polycyclic aromatic compound production and to decreased richness and diversity of bacterial communities. We showed a rapid impact of the rhizosphere on PAH degradation via the increased activity of PAH-degrading bacteria. After 12 days, PAH degradation was significantly higher in the planted (100% degradation) than in unplanted (70%) soil. Gram-negative (Proteobacteria) PAH-dioxygenase genes and transcripts were higher in planted than unplanted soil and were correlated to the spiked PAH degradation. Conversely, Gram-positive (Actinobacteria) PAH-dioxygenase gene transcription was constant over time in both conditions. At 12 days, plant growth favored the activity of many Gammaproteobacteria (Pseudomonadaceae, Stenotrophomonas, and Acinetobacter) while in unplanted soil Alphaproteobacteria (Sphingomonadaceae, Sphingobium, and Magnetospirillum) and Actinobacteria (Iamia, Geodermatophilaceae, and Solirubrobacterales) were more active.

The molecular mechanisms that promote pathologic alterations in human physiology mediated by short-term exposure to traffic pollutants remains not well understood. This work was to develop mechanistic networks to determine which specific pathways are activated by real-world exposures of traffic-related air pollution (TRAP) during rest and moderate physical activity (PA). A controlled crossover study to compare whole blood gene expression pre and post short-term exposure to high and low of TRAP was performed together with systems biology analysis. Twenty-eight healthy volunteers aged between 21 and 53 years were recruited. These subjects were exposed during 2 h to different pollution levels (high and low TRAP levels), while either cycling or resting. Global transcriptome profile of each condition was performed from human whole blood samples. Microarrays analysis was performed to obtain differential expressed genes (DEG) to be used as initial input for GeneMANIA software to obtain protein-protein (PPI) networks. Two networks were found reflecting high or low TRAP levels, which shared only 5.6 and 15.5% of its nodes, suggesting specific cell signaling pathways being activated in each environmental condition. However, gene ontology analysis of each PPI network suggests that each level of TRAP regulate common members of NF-κB signaling pathway. Our work provides the first approach describing mechanistic networks to understand TRAP effects on a system level.

Poultry manure (PM), a rich source for crop nutrients, is produced in ample quantities worldwide. It provides necessary nutrient to soil and has a potential to improve plant water holding availability under semiarid environment. The effect of composted poultry manure (CPM) and irrigation regimes on morpho-physiology of selective maize (Zea mays L.) hybrids (H₁ = drought tolerant, H₂ = drought sensitive) was investigated in this study. Two field experiments were conducted during 2010 and 2011 under randomized complete block design with split split-plot arrangements and three replications of each treatment. Irrigation regimes (I₁ = 300, I₂ = 450, I₃ = 600 mm) were kept in main plots; the two maize hybrids (H₁ and H₂) in sub-plots and nutrient levels (L₁ = recommended rate of NPK (control), L₂ = 8 t ha⁻¹ CPM, L₃ = 10 t ha⁻¹ CPM, and L₄ = 12 t ha⁻¹ CPM) were arranged in sub sub-plots. The drought tolerant hybrid showed best growth under all treatments. Results revealed that maximum leaf area index (LAI) was recorded with the application of the recommended dose of NPK. Low irrigation regimes (I₁ and I₂) highly significantly (P < 0.01) reduced the photosynthesis and transpiration rate in both hybrids while application of 12 t ha⁻¹ CPM was able to partially alleviate the effect of water stress on these parameters. Resultantly, the application of 12 t ha⁻¹ CPM enhanced the plant growth and increased grain yield (21%; 4.17 vs 5.27) under limited water availability (I₂L₄) as compared to the recommended dose of NPK (I₂L₁). However, the nutrient application under control treatment had maximum grain yield. Therefore, shortage of water for maize production might be partially alleviated by the application of 12 t ha⁻¹ CPM.

Pollutants have been proposed as one factor in the worldwide declines of amphibian species and populations. Applying gene expression analysis of liver RNA in tadpoles would be a possible approach for biomarker measurements to increase knowledge of ecological health in amphibian populations. The major aim of this study was to explore the relevance of applying gene expression analyses of cytochrome p450 (cyp1a), metallothionein (mt), and vitellogenin (vtg) in Rana temporaria tadpoles. Therefore, tadpoles were exposed for 1 week to β-naphthoflavone (BNF), cadmium chloride (CdCl₂), and ethinylestradiol (EE2). Primers were developed for RT-qPCR to analyze gene expression in livers. The result showed that the methods for gene expression analyses of cyp1a, mt, and vtg as well as the reference gene β-actin (bact) were successful not only in R. temporaria but also in another amphibian, Rana arvalis. The gene expression of cyp1a was induced by BNF and the gene expression of mt was induced by CdCl₂ but no significant induction was recorded in vtg expression after exposure to EE2. Gene expressions varied throughout the tadpole metamorphosis development, in particular for vtg. Overall, the use of gene expression of cyp1a and mt as biomarkers in wild tadpoles seems promising while the use of vtg seems less relevant due to high natural variation and low background expression. The study shows that variations in gene expressions between tadpoles of different genetic origin are important to consider when evaluating the data. The present study has thus increased the background knowledge about gene expression applicability as biomarker for tadpoles.

Cocoa production is affected by the black pod disease caused by several Phytophthora species that bring, about each year, an estimated loss of 44% of world production. Chemical control remains expensive and poses an enormous risk of poisoning for the users and the environment. Biocontrol by using antagonistic microorganisms has become an alternative to the integrated control strategy against this disease. Trichoderma viride T7, T. harzanium T40, and T. asperellum T54, which showed in vivo and in vitro antagonistic activity against P. palmivora, were cultured and mycelia extracted. Inhibition activity of crude extracts was determined, and then organic compounds were isolated and characterized. The in vitro effect of each compound on the conidia germination and mycelia growth of four P. palmivora, two P. megakaria, and one P. capsici was evaluated. T. viride that displayed best activities produced two active metabolites, viridin and gliovirin, against P. palmivora and P. megakaria strains. However, no activity against P. capsici was observed. Besides being active separately, these two compounds have a synergistic effect for both inhibitions, mycelia growth and conidia germination. These results provide the basis for the development of a low-impact pesticide based on a mixture of viridin and gliovirine.