Flow cytometry has recently been presented as a research tool in the assessment of the viability/activity of activated sludge from municipal wastewater treatment plants, but it has not put in practice for industrial biotreatments yet. In this study, for the first time ever, the reliability and significance of the multiparameter flow cytometry applied to the biological nitrification-denitrification treatment of leachate have been evaluated. Using a double staining procedure (cFDA/PI), the viable, damaged, and dead subpopulations were determined, and the results were compared to those obtained with conventional methods, such as nitrogen and oxygen uptake rates or plate counting. Flow cytometry showed that viable cells represented approximately 47% of the total population, whereas active cells accounted for 90%. For both sludge from nitrification and denitrification processes, with less than 1% of them being also culturable in plate. Either flow cytometry or uptake rates revealed that health status of sludge remained constant throughout the biotreatment, which is consistent with the high recirculation rates. Under anaerobic starvation conditions, physiological status of sludge remained constant as well as specific oxygen and denitrification rates. Nevertheless, both the culturability in plate and the nitrification rate significantly decreased. These findings proved that multiparameter flow cytometry is a useful tool for the assessment of the viability and activity of sludge from a nitrification-denitrification biotreatment process. These results gathered all the bacterial communities in the sludge, so the decay in minority populations, such as nitrifying bacteria, requires the use of a complementary technique to evaluate specific activities.

The detailed analysis of columnar optical and microphysical properties of aerosols obtained from the AErosol RObotic NETwork (AERONET) Cimel sun photometer operated at Skukuza (24.98° S, 31.60° E, 150 m above sea level), South Africa was carried out using the level 2.0 direct sun and inversion products measured during 1999–2010. The observed aerosol optical depth (AOD) was generally low over the region, with high values noted in late winter (August) and mid-spring (September and October) seasons. The major aerosol types found during the study period were made of 3.74, 69.63, 9.34, 8.83, and 8.41% for polluted dust (PD), polluted continental (PC), non-absorbing (NA), slightly absorbing (SA), and moderately absorbing (MA) aerosols, respectively. Much attention was given to the aerosol fine- and coarse-modes deduced from the particle volume concentration, effective radius, and fine-mode volume fraction. The aerosol volume size distribution pattern was found to be bimodal with the fine-mode showing predominance relative to coarse-mode during the winter and spring seasons, owing to the onset of the biomass burning season. The mean values of total, fine-, and coarse-mode volume particle concentrations were 0.07 ± 0.04, 0.03 ± 0.03, and 0.04 ± 0.02 μm³ μm⁻², respectively, whereas the mean respective effective radii observed at Skukuza for the abovementioned modes were 0.35 ± 0.17, 0.14 ± 0.02, and 2.08 ± 0.02 μm. The averaged shortwave direct aerosol radiative forcing (ARF) observed within the atmosphere was found to be positive (absorption or heating effect), whereas the negative forcing in the surface and TOA depicted significant cooling effect due to more scattering type particles.

With the increasing of high saline waste sludge production, the treatment and utilization of saline waste sludge attracted more and more attention. In this study, the biological hydrogen production from saline waste sludge after heating pretreatment was studied. The substrate metabolism process at different salinity condition was analyzed by the changes of soluble chemical oxygen demand (SCOD), carbohydrate and protein in extracellular polymeric substances (EPS), and dissolved organic matters (DOM). The excitation-emission matrix (EEM) with fluorescence regional integration (FRI) was also used to investigate the effect of salinity on EPS and DOM composition during hydrogen fermentation. The highest hydrogen yield of 23.6 mL H₂/g VSS and hydrogen content of 77.6% were obtained at 0.0% salinity condition. The salinity could influence the hydrogen production and substrate metabolism of waste sludge.

Increased atmospheric nitrogen (N) deposition is known to alter ecosystem carbon source-sink dynamics through changes in soil CO₂ fluxes. However, a limited number of experiments have been conducted to assess the effects of realistic N deposition in the Mediterranean Basin, and none of them have explored the effects of N addition on soil respiration (R ₛ). To fill this gap, we assessed the effects of N supply on R ₛ dynamics in the following two Mediterranean sites: Capo Caccia (Italy), where 30 kg ha⁻¹ year⁻¹ was supplied for 3 years, and El Regajal (Spain), where plots were treated with 10, 20, or 50 kg N ha⁻¹ year⁻¹ for 8 years. Results show a complex, non-linear response of soil respiration (R ₛ) to N additions with R ₛ overall increasing at Capo Caccia and decreasing at El Regajal. This suggests that the response of R ₛ to N addition depends on dose and duration of N supply, and the existence of a threshold above which the N introduced in the ecosystem can affect the ecosystem’s functioning. Soil cover and seasonality of precipitations also play a key role in determining the effects of N on R ₛ as shown by the different responses observed across seasons and in bare soil vs. the soil under canopy of the dominant species. These results show how increasing rates of N addition may influence soil C dynamics in semiarid ecosystems in the Mediterranean Basin and represent a valuable contribution for the understanding and the protection of Mediterranean ecosystems.

The aim of this work is to study the atmospheric concentrations of selected major and trace elements and ions found in PM₂.₅, at a suburban site in Athens, Greece, and discuss on the impact of the different sources. Special focus is given to the influence of Saharan dust episodes. The seasonal variability in the metal and ion concentrations is also examined. The results show that PM₂.₅ mass concentrations are significantly influenced by Saharan dust events; it is observed that when the PM₂.₅ concentration is higher than 25 μg/m³, five out of six times, the air mass crossed North Africa at an altitude within the boundary layer. Fe is found to be the element with the more significant seasonal variability, displaying much higher concentrations during cold period. The frequent Saharan dust intrusions in the cold period of this dataset may explain this result. Mineral dust and secondary aerosol are the main PM₂.₅ components (29 and 34%, respectively). During Saharan dust events, the concentration of mineral dust is increased by 35% compared to the days without dust intrusions, while an increase of 68% of the sea salt is also observed. During event days, PM₂.₅ concentrations are also increased by 14%. Anthropogenic components do not decrease during those days, while sulfate displays even a slight increase, suggesting enrichment of mineral dust with secondary sulfates. The results indicate that African dust intrusions add a rather significant PM pollution load even in the PM₂.₅ fraction, with implication to population exposure and human health.

The aim of this study was to analyze the occurrence of sulfonamide resistance genes (sul1–3) and other genetic elements as antiseptic resistance gene (qacEΔ1) and class 1 and class 2 integrons (int1–2) in the upper layer of substrate and in the effluent of microcosm constructed wetlands (CWs) treating artificial wastewater containing diclofenac and sulfamethoxazole (SMX), which is a sulfonamide antibiotic. The bacteria in the substrate and in the effluents were equipped with the sul1–2, int1, and qacEΔ1 resistance determinants, which were introduced into the CW system during inoculation with activated sludge and with the soil attached to the rhizosphere of potted seedlings of Phalaris arundinacea ‘Picta’ roots (int1). By comparing the occurrence of the resistance determinants in the upper substrate layer and the effluent, it can be stated that they neither were lost nor emerged along the flow path. The implications of the presence of antibiotic resistance genes in the effluent may entail a risk of antibiotic resistance being spread in the receiving environment. Additionally, transformation products of SMX were determined. According to the obtained results, four (potential) SMX transformation products were identified. Two major metabolites of SMX, 2,3,5-trihydroxy-SMX and 3,5-dihydroxy-SMX, indicated that SMX may be partly oxidized during the treatment. The remaining two SMX transformation products (hydroxy-glutathionyl-SMX and glutathionyl-SMX) are conjugates with glutathione, which suggests the ability of CW bacterial community to degrade SMX and resist antimicrobial stress.

Concentrations of metals in household dust samples from rural, semi-urban and urban zones of the Niger Delta in Nigeria were measured during both 2009 and 2014 with the aim of providing information on changes in the concentrations, distribution patterns, sources and risks of metals in these zones. The concentrations of metals in the dust samples were quantified by using inductively coupled plasma atomic emission spectrophotometry (ICP-AES) after digestion with aqua regia. The measured concentrations (mg kg⁻¹) of metals in the three zones within the study periods were as follows: <LOQ–21.2; <LOQ–182; 7.90–265; <LOQ–117; <LOQ–471; 3.37–2310; 0.35–7.9; 4.25–365; 6.78–61,600; 219–37,700; and 1180–18,000 for Cd, Pb, Ba, Cr, Ni, Cu, Co, Mn, Zn, Fe and Al, respectively. The results from the two periods indicate significant changes in the concentrations, distribution patterns and risk factors which reflects a deterioration of the quality of the household environment over this time span. The hazard index (HI) values calculated for children were greater than 1 indicating significant non-cancer risks for these subjects in these areas. The HI values for adults were less than 1 and consequently do not pose a significant risk. The carcinogenic risk levels for exposure to metals for both adults and children in these zones were below the range specified as safe by the US EPA (×10⁻⁶ and ×10⁻⁴) thereby indicating a low cancer risk. The sources of metals in household dust from these zones include emissions from industries, traffic, artisanal workshops and releases from household furniture, metal-based fittings, metal roofing and pesticides.

Present-day climate change scenario has intensified the problem of continuously increasing ground-level ozone (O₃), which is responsible for causing deleterious effects on growth and development of plants. Studies involving use of ethylenediurea (EDU), a chemical with antiozonant properties, have given some promising results in evaluating O₃ injury in plants. The use of EDU is especially advantageous in developing countries which face a more severe problem of ground-level O₃, and technical O₃-induced yield loss assessment techniques like open-top chambers cannot be used. Recent studies have detected a hormetic response of EDU on plants; i.e. treatment with higher EDU concentrations may or may not show any adverse effect on plants depending upon the experimental conditions. Although the mode of action of EDU is still debated, it is confirmed that EDU remains confined in the apoplastic regions. Certain studies indicate that EDU significantly affects the electron transport chain and has positive impact on the antioxidant defence machinery of the plants. However, the mechanism of protecting the yield of plants without significantly affecting photosynthesis is still questionable. This review discusses in details the probable mode of action of EDU on the basis of available data along with the impact of EDU on physiological, biochemical, growth and yield response of plants under O₃ stress. Data regarding the effect of EDU on plant ‘omics’ is highly insufficient and can form an important aspect of future EDU research.

In this study, the optimum design of the entrance of a fishpond laterally to the main flow of an open channel was investigated numerically and experimentally. The flow characteristic measurements were realized with the PIV (particle image velocimetry) method. The mathematical simulations were based on the development of a two dimensional -mean in depthhydrodynamic model and a quasi three dimensional sediment transport model which includes processes of advection, diffusion, and settling of conservative suspended matter. The study was completed with the comparison of the final results of the mathematical models with the findings of the physical model revealing the hydrodynamic interaction and coupling between the main flow of the channel and the lateral reservoir—fishpond and leading to the optimum technical design of the system.

Pulse inputs of heavy metals are expected to increase with a higher frequency of extreme climate events (heavy rain), leading to stronger erosion of contaminated and fertilized farmland soils to freshwaters, with potentially adverse effects on lake ecosystems. We conducted a 5-month mesocosm study to elucidate the responses of the submerged macrophyte Vallisneria spinulosa and phytoplankton to four different doses of cadmium (Cd): 0 (control), 0.05, 0.5, and 5 g m⁻² (CK, I, II, and III, respectively) under mesotrophic conditions. We found that total phosphorus concentrations were larger in the three Cd pulse treatments, whereas total nitrogen concentrations did not differ among the four treatments. The contents of chlorophyll a and soluble sugar in macrophyte leaves decreased in III, and total biomass, ramet number, plant height, and total stolon length of macrophytes were lower in both II and III. In contrast, abundances of the three main phytoplankton taxa—Cyanophyta, Chlorophyta, and Bacillariophyta—did not differ among treatments. Total phytoplankton biomass was, however, marginally lower in CK than in the Cd treatments. We conclude that exposure to strong Cd pulses led to significantly reduced growth of macrophytes, while no obvious effect appeared for phytoplankton.