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Exposure of juvenile Danio rerio to aged TiO2 nanomaterial from sunscreen Texte intégral
2013
Fouqueray, Manuela | Noury, Patrice | Dherret, Lysiane | Chaurand, Perrine | Abbaci, Khedidja | Labille, Jérôme | Rose, Jérôme | Garric, Jeanne | Milieux aquatiques, écologie et pollutions (UR MALY) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Centre Européen de Recherche et d'Enseignement des Géosciences de l'Environnement (CEREGE) ; Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)
[Departement_IRSTEA]Eaux [TR1_IRSTEA]BELCA | International audience | The toxicity of dietary exposure to artificially aged TiO2 nanomaterial (T-Lite (TM)) used in sunscreen cream was studied on Danio rerio. Embryolarval assays were conducted to assess the effects of TiO2 residues of nanomaterial (RNM) on fish early life stages. Juvenile fishes were exposed by the trophic route in two experiments. During the first experiment, juvenile fishes were exposed to TiO2 RNM for 14 days by adding RNM to commercial fish food. The second one consisted in producing a trophic food chain. Pseudokirchneriella subcapitata algae, previously contaminated with TiO2 RNM in growth medium, was used to feed Daphnia magna neonates over a 48-h period. Daphnia were used next to feed juvenile fishes for 7 days. Accumulation of Ti, life traits (survival and growth) and biochemical parameters such as energy reserves, digestive (trypsin, esterase, cellulose and amylase) and antioxidant (superoxide dismutase and catalase) enzyme activity were measured at the end of exposures. As expected in the receiving aquatic system, TiO2 RNM at low concentrations caused a low impact on juvenile zebrafish. A slight impact on the early life stage of zebrafish with premature hatching was observed, and this effect appeared mainly indirect, due to possible embryo hypoxia. When juvenile fish are exposed to contaminated food, digestive enzyme activity indicated a negative effect of TiO2 RNM. Digestive physiology was altered after 14 days of exposure and seemed to be an indirect target of TiO2 RNM when provided by food.
Afficher plus [+] Moins [-]Risk assessment of herbicides and booster biocides along estuarine continuums in the Bay of Vilaine area (Brittany, France) | L'évaluation du risque écotoxique d'herbicides et de biocides le long du continuum de l'estuaire de la baie de Vilaine (Bretagne, France) Texte intégral
2013
Caquet, Thierry | Roucaute, Marc | Mazzella, Nicolas | Delmas, François | Madigou, Céline | Farcy, E. | Burgeot, Thierry | Allenou, J.P. | Gabellec, R. | Institut National de la Recherche Agronomique (INRA) | Réseaux épuration et qualité des eaux (UR REBX) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Hydrosystèmes et Bioprocédés (UR HBAN) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
[Departement_IRSTEA]Eaux [TR1_IRSTEA]BELCA | International audience | Estuarine areas represent complex and highly changing environments at the interface between freshwater and marine aquatic ecosystems. Therefore, the aquatic organisms living in estuaries have to face highly variable environmental conditions. The aimof this work was to study the influence of environmental changes from either natural or anthropogenic origins on the physiological responses of Mytilus edulis. Mussels were collected in theVilaine estuary during early summer because this season represents a critical period of active reproduction in mussels and of increased anthropogenic inputs from agricultural and boating activities into the estuary. The physiological status of the mussel M. edulis was evaluated through measurements of a suite of biomarkers related to: oxidative stress (catalase, malondialdehyde), detoxication (benzopyrene hydroxylase, carboxylesterase), neurotoxicity (acetylcholinesterase), reproductive cycle (vitelline, condition index, maturation stages), immunotoxicity (hemocyte concentration, granulocyte percentage, phagocytosis, reactive oxygen species production, oxidative burst), and general physiological stress (lysosomal stability). A selection of relevant organic contaminant (pesticides, (polycyclic aromatic hydrocarbons, polychlorobiphenyls) was measured as well as environmental parameters (water temperature, salinity, total suspended solids, turbidity, chlorophyll a, pheopigments) and mussel phycotoxin contamination. Two locations differently exposed to the plume of the Vilaine River were compared. Both temporal and inter-site variations of these biomarkers were studied. Our results show that reproduction cycle and environmental parameters such as temperature, organic ontaminants, and algal blooms could strongly influence the biomarker responses. These observations highlight the necessity to conduct integrated environmental approaches in order to better understand the causes of biomarker variations.
Afficher plus [+] Moins [-]Hybrid larch (Larix x eurolepis Henry): a good candidate for cadmium phytoremediation? Texte intégral
2013
Moudouma, Chris Fabien Moussavou | Riou, Catherine | Gloaguen, Vincent | Saladin, Gaëlle | Laboratoire de Chimie des Substances Naturelles (LCSN) ; Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503) | Laboratoire des interactions plantes micro-organismes (LIPM) ; Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) | Université de Limoges (UNILIM) | Eau Environnement Limoges (E2Lim) ; Institut Matériaux Procédés Environnement Ouvrages (IMPEO) ; Université de Limoges (UNILIM)-Université de Limoges (UNILIM)
International audience
Afficher plus [+] Moins [-]Biofiltration vs conventional activated sludge plants: what about priority and emerging pollutants removal? Texte intégral
2013
Mailler, Romain | Gasperi, Johnny | Rocher, V. | Gilbert-Pawlik, S. | Geara-Matta, D. | Moilleron, R. | Chebbo, G. | Laboratoire Eau, Environnement et Systèmes Urbains (LEESU) ; AgroParisTech-Université Paris-Est Marne-la-Vallée (UPEM)-École nationale des ponts et chaussées (ENPC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) | SIAAP - Direction du Développement et de la Prospective ; Syndicat interdépartemental pour l'assainissement de l'agglomération parisienne (SIAAP) | Projet OPUR
International audience | : This paper compares the removal performances of two complete wastewater treatment plants (WWTPs) for all priority substances listed in the Water Framework Directive and additional compounds of interest including flame retardants, surfactants, pesticides, and personal care products (PCPs) (n = 104). First, primary treatments such as physicochemical lamellar settling (PCLS) and primary settling (PS) are compared. Similarly, biofiltration (BF) and conventional activated sludge (CAS) are then examined. Finally, the removal efficiency per unit of nitrogen removed of both WWTPs for micropollutants is discussed, as nitrogenous pollution treatment results in a special design of processes and operational conditions. For primary treatments, hydrophobic pollutants (log K ow > 4) are well removed (>70 %) for both systems despite high variations of removal. PCLS allows an obvious gain of about 20 % regarding pollutant removals, as a result of better suspended solids elimination and possible coagulant impact on soluble compounds. For biological treatments, variations of removal are much weaker, and the majority of pollutants are comparably removed within both systems. Hydrophobic and volatile compounds are well (>60 %) or very well removed (>80 %) by sorption and volatilization. Some readily biodegradable molecules are better removed by CAS, indicating a better biodegradation. A better sorption of pollutants on activated sludge could be also expected considering the differences of characteristics between a biofilm and flocs. Finally, comparison of global processes efficiency using removals of micropollutants load normalized to nitrogen shows that PCLS + BF is as efficient as PS + CAS despite a higher compactness and a shorter hydraulic retention time (HRT). Only some groups of pollutants seem better removed by PS + CAS like alkylphenols, flame retardants, or di-2-ethylhexyl phthalate (DEHP), thanks to better biodegradation and sorption resulting from HRT and biomass characteristics. For both processes, and out of the 68 molecules found in raw water, only half of them are still detected in the water discharged, most of the time close to their detection limit. However, some of them are detected at higher concentrations (>1 μg/L and/or lower than environmental quality standards), which is problematic as they represent a threat for aquatic environment.
Afficher plus [+] Moins [-]Pesticide pressure and fish farming in barrage pond in northeastern France. Part III: how management can affect pesticide profiles in edible fish? Texte intégral
2013
Lazartigues, Angélique | Banas, Damien | Feidt, Cyril | Brun-Bellut, Jean | Gardeur, Jean-Noel | Le Roux, Yves | Thomas, Marielle | Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | Agence de l'Eau Rhin-Meuse; Zone Atelier Moselle
International audience | The quality of fish produced in ponds needs to be ensured. Indeed, pond is often strongly connected to an agricultural watershed, and pesticides are a main health and environmental issue of concern. In this context, the purpose of this study is to highlight the management practices which could impact the pesticide contamination profiles in edible fish and to give recommendations for better practices. A principal component analysis, coupled to a hierarchical cluster analysis, was performed to evaluate temporal evolution of contamination profiles and to assess variability among fish species and among sites according to watershed characteristics. The explicative variables correspond to muscular concentrations of pesticides (azoxystrobin, clomazone, diflufenican, carbendazim, isoproturon, metazachlor, napropamid) in three species of fish (Perca fluviatilis, Cyprinus carpio and Rutilus rutilus), caught in five ponds during two sampling campaigns. Management data are added variables in order to discuss about parameters suspected to be implicated in the contamination profiles recorded. This work shows that high amounts of pesticides applied, short crop rotation durations and bare soil practices led to contamination of sediments and fish and were associated to a ``bad'' management of watershed. Breeding fish that had low masses and establishing the fishing period at the end of winter seemed to be ``bad'' management of pond. Aggravating topological parameters were big watershed coupled to small pond and high proportions of sand soils in the watershed. Reducing amounts of pesticide used (e.g. policy agency plans, farmer acceptance), favouring long-term rotations and inter-cultures, adapting pond creation and fish farming practices to watershed management and topography all could reduce pesticide levels in edible fish and contribute to a better sustainability of the extensive fish farming in pond.
Afficher plus [+] Moins [-]Pesticide risk assessment and management in a globally changing world. Report from a European interdisciplinary workshop Texte intégral
2013
Babut, Marc | Arts, Gertie H. | Caracciolo, Anna Barra | Carluer, Nadia | Domange, Nicolas | Friberg, Nikolai | Gouy, Véronique | Grung, Merete | Lagadic, Laurent | Martin-Laurent, Fabrice | Mazzella, Nicolas | Pesce, Stéphane | Real, Benoit | Reichenberger, Stefan | Roex, Erwin W. M. | Romijn, Kees | Röttele, Manfred | Stenrod, Marianne | Tournebize, Julien | Vernier, Françoise | Vindimian, Eric | Milieux aquatiques, écologie et pollutions (UR MALY) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Centre for Ecosystem studies ; ALTERRA | National Research Council ; Water Research Institute | Office national de l'eau et des milieux aquatiques (ONEMA) | Aarhus University [Aarhus] | Norwegian Institute for Water Research (NIVA) | Ecobiologie et qualité des hydrosystèmes continentaux (EQHC) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Rennes | Agroécologie [Dijon] ; Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement | Réseaux épuration et qualité des eaux (UR REBX) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Service Agronomie Economie Environnement ; ARVALIS - Institut du végétal [Paris] | aucun ; Footways | Deltares | Environmental Sciences ; Bayer S.A.S. [France] ; Bayer AG [Germany]-Bayer AG [Germany] | aucun ; Better Decisions | Department of Ecology and Natural Resource Management ; Norwegian University of Life Sciences (NMBU) | Hydrosystèmes continentaux anthropisés : ressources, risques, restauration (UR HYCAR) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Aménités et dynamiques des espaces ruraux (UR ADBX) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Services généraux (SGMO) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Irstea (Scientific and International Affairs Offices)
Pesticide risk assessment and management in a globally changing world. Report from a European interdisciplinary workshop Texte intégral
2013
Babut, Marc | Arts, Gertie H. | Caracciolo, Anna Barra | Carluer, Nadia | Domange, Nicolas | Friberg, Nikolai | Gouy, Véronique | Grung, Merete | Lagadic, Laurent | Martin-Laurent, Fabrice | Mazzella, Nicolas | Pesce, Stéphane | Real, Benoit | Reichenberger, Stefan | Roex, Erwin W. M. | Romijn, Kees | Röttele, Manfred | Stenrod, Marianne | Tournebize, Julien | Vernier, Françoise | Vindimian, Eric | Milieux aquatiques, écologie et pollutions (UR MALY) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Centre for Ecosystem studies ; ALTERRA | National Research Council ; Water Research Institute | Office national de l'eau et des milieux aquatiques (ONEMA) | Aarhus University [Aarhus] | Norwegian Institute for Water Research (NIVA) | Ecobiologie et qualité des hydrosystèmes continentaux (EQHC) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Rennes | Agroécologie [Dijon] ; Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement | Réseaux épuration et qualité des eaux (UR REBX) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Service Agronomie Economie Environnement ; ARVALIS - Institut du végétal [Paris] | aucun ; Footways | Deltares | Environmental Sciences ; Bayer S.A.S. [France] ; Bayer AG [Germany]-Bayer AG [Germany] | aucun ; Better Decisions | Department of Ecology and Natural Resource Management ; Norwegian University of Life Sciences (NMBU) | Hydrosystèmes continentaux anthropisés : ressources, risques, restauration (UR HYCAR) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Aménités et dynamiques des espaces ruraux (UR ADBX) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Services généraux (SGMO) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Irstea (Scientific and International Affairs Offices)
[Departement_IRSTEA]Eaux [TR1_IRSTEA]BELCA [Axe_IRSTEA]DTAM-QT2-ADAPTATION [TR2_IRSTEA]ARCEAU [TR2_IRSTEA]DTAM | Global climate change will affect worldwide agriculture in many ways. The anticipated or already occurring changes raise concerns about the sustainability of production and the ability of agriculture to feed human populations. This appeals to sustainable agriculture providing ecosystem services more efficiently than today, and accordingly to substantial evolutions of pesticide risk assessment (RA) and risk management (RM). The RA/RM issues were discussed by two European research networks in a 2011 workshop. The RA-RM-monitoring conceptual cycle tends to be virtual, with poor connections between certain steps. The design of more comprehensive emissions scenarios could improve the accuracy of predicted runoff transport, while the microcosm/mesocosm approach could help establish causal relationships between fate / exposure and populations / communities. Combined with ecological modelling, effects can be extrapolated to higher spatial and temporal scales. Risk management of diffuse sources should be designed simultaneously at the watershed and individual plot scales. Monitoring is key to assessing the effectiveness of risk reduction measures reduce and evaluate the overall quality of the aquatic compartment. More flexible monitoring strategies clearly linked to RM decisions are therefore needed. Although some technical questions remain, it is time to apply passive samplers more routinely. A set of research and development needs covering the whole RA/RM cycle is listed in conclusion.
Afficher plus [+] Moins [-]Pesticide risk assessment and management in a globally changing world—report from a European interdisciplinary workshop Texte intégral
2013
Babut, Marc | Arts, Gertie H. | Caracciolo, Anna Barra | Carluer, Nadia | Domange, Nicolas | Friberg, Nikolai | Gouy, Vèronique | Grung, Merete | Lagadick, Laurent | Martin-Laurent, Fabrice | Mazzella, Nicolas | Pesce, Stèphane | Real, Benoit | Reichenberger, Stefan | Roex, Erwin W. M. | Romijn, Kees | Röttele, Manfred | Stenrød, Marianne | Tounebize, Julien | Vernier, Francoise | Vindimian, Eric
Relative bioavailability of tropical volcanic soil-bound chlordecone in laying hens (Gallus domesticus) Texte intégral
2013
Lesueur-Jannoyer, Magalie | Rychen, Guido | Feidt, Cyril | Jondreville, Catherine | Bouveret, Cecile | Fonctionnement agroécologique et performances des systèmes de cultures horticoles (UPR HORTSYS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Campus agro-environnemental Caraïbe (CAEC) | Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | French National Chlordecone Plan
International audience | The former use of chlordecone (CLD) in the French West Indies has resulted in long-term pollution of soils and of food chains. CLD may be transferred into eggs of hens reared outdoors, through polluted soil ingestion. Tropical volcanic soils display variable capacities of pollutant retention: CLD is less available and more persistent in andosol than in nitisol. The impact of soil type on CLD bioavailability to hens was tested through a relative bioavailability study. The deposition of CLD in egg yolk and in abdominal fat was measured in 42 individually housed laying hens fed with diets containing graded levels of CLD from polluted andosol, nitisol, or spiked oil during 23 days. Within each ingested matrix, the concentration of CLD in yolk and in abdominal fat linearly increased with the amount of ingested CLD (P < 0.001). However, the response to andosol diets and to nitisol diets was not different from the response to oil diets (P > 0.1), indicating that CLD was equally bioavailable to laying hens, irrespective of the matrix. This suggests that the hen's gastrointestinal tract efficiently extracts CLD from the two tropical volcanic soils, regardless of their retention capacity. Thus, hens reared on polluted soils with CLD may lay contaminated eggs.
Afficher plus [+] Moins [-]An integrated environmental approach to investigate biomarker fluctuations in the blue mussel Mytilus edulis L. in the Vilaine estuary, France. Texte intégral
2013
Farcy, Emilie | Burgeot, Thierry | Haberkorn, Hansy | Auffret, Michel | Lagadic, Laurent | Allenou, Jean-Pierre | Budzinski, Hélène | Mazzella, Nicolas | Pete, Romain | Heydorff, Micheline | Menard, Dominique | Mondeguer, Florence | Caquet, Thierry | Unité Biogéochimie et Ecotoxicologie (BE) ; Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) | Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) ; Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) | Écologie et santé des écosystèmes (ESE) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Laboratoire Environnement Ressources Morbihan Pays de Loire (LERMPL) ; Unité Observation et écologie de la restauration des écosystèmes littoraux (COAST) ; Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) | Laboratoire de Physico et Toxico-Chimie des systèmes naturels (LPTC) ; Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS) | Réseaux épuration et qualité des eaux (UR REBX) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Ecologie des systèmes marins côtiers (Ecosym) ; Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire Phycotoxines (PHYC) ; Unité Dynamiques des Écosystèmes Côtiers (DYNECO) ; Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) | French National Research Agency
International audience | Estuarine areas represent complex and highly changing environments at the interface between freshwater and marine aquatic ecosystems. Therefore, the aquatic organisms living in estuaries have to face highly variable environmental conditions. The aim of this work was to study the influence of environmental changes from either natural or anthropogenic origins on the physiological responses of Mytilus edulis. Mussels were collected in the Vilaine estuary during early summer because this season represents a critical period of active reproduction in mussels and of increased anthropogenic inputs from agricultural and boating activities into the estuary. The physiological status of the mussel M. edulis was evaluated through measurements of a suite of biomarkers related to: oxidative stress (catalase, malondialdehyde), detoxication (benzopyrene hydroxylase, carboxylesterase), neurotoxicity (acetylcholinesterase), reproductive cycle (vitelline, condition index, maturation stages), immunotoxicity (hemocyte concentration, granulocyte percentage, phagocytosis, reactive oxygen species production, oxidative burst), and general physiological stress (lysosomal stability). A selection of relevant organic contaminant (pesticides, (polycyclic aromatic hydrocarbons, polychlorobiphenyls) was measured as well as environmental parameters (water temperature, salinity, total suspended solids, turbidity, chlorophyll a, pheopigments) and mussel phycotoxin contamination. Two locations differently exposed to the plume of the Vilaine River were compared. Both temporal and inter-site variations of these biomarkers were studied. Our results show that reproduction cycle and environmental parameters such as temperature, organic ontaminants, and algal blooms could strongly influence the biomarker responses. These observations highlight the necessity to conduct integrated environmental approaches in order to better understand the causes of biomarker variations.
Afficher plus [+] Moins [-]Risk assessment of herbicides and booster biocides along estuarine continuums in the Bay of Vilaine area (Brittany, France) Texte intégral
2013
Caquet, Thierry | Roucaute, Marc | Mazzella, Nicolas | Delmas, François | Madigou, C. | Farcy, E. | Burgeot, Th. | Allenou, J. -P. | Gabellec, R. | Écologie et santé des écosystèmes (ESE) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Réseaux épuration et qualité des eaux (UR REBX) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Unité Biogéochimie et Ecotoxicologie (BE) ; Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) | Laboratoire Environnement Ressources Morbihan Pays de Loire (LERMPL) ; Unité Observation et écologie de la restauration des écosystèmes littoraux (COAST) ; Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) | French National Research Agency in the framework of the program Ecotoxicology and Ecodynamics of Contaminants (ECODYN)
A 2-year study was implemented to characterize the contamination of estuarine continuums in the Bay of Vilaine area (NW Atlantic Coast, Southern Brittany, France) by 30 pesticide and biocide active substances and metabolites. Among these, 11 triazines (ametryn, atrazine, desethylatrazine, desethylterbuthylazine, desisopropyl atrazine, Irgarol 1051, prometryn, propazine, simazine, terbuthylazine, and terbutryn), 10 phenylureas (chlortoluron, diuron, 1-(3,4-dichlorophenyl)-3-methylurea, fenuron, isoproturon, 1-(4-isopropylphenyl)-3-methylurea, 1-(4-isopropylphenyl)-urea, linuron, metoxuron, and monuron), and 4 chloroacetanilides (acetochlor, alachlor, metolachlor, and metazachlor) were detected at least once. The objectives were to assess the corresponding risk for aquatic primary producers and to provide exposure information for connected studies on the responses of biological parameters in invertebrate sentinel species. The risk associated with contaminants was assessed using risk quotients based on the comparison of measured concentrations with original species sensitivity distribution-derived hazardous concentration values. For EU Water Framework Directive priority substances, results of monitoring were also compared with regulatory Environmental Quality Standards. The highest residue concentrations and risks for primary producers were recorded for diuron and Irgarol 1051 in Arzal reservoir, close to a marina. Diuron was present during almost the all survey periods, whereas Irgarol 1051 exhibited a clear seasonal pattern, with highest concentrations recorded in June and July. These results suggest that the use of antifouling biocides is responsible for a major part of the contamination of the lower part of the Vilaine River course for Irgarol 1051. For diuron, agricultural sources may also be involved. The presence of isoproturon and chloroacetanilide herbicides on some dates indicated a significant contribution of the use of plant protection products in agriculture to the contamination of Vilaine River. Concentration levels and associated risk were always lower in estuarine sites than in the reservoir, suggesting that Arzal dam reduces downstream transfer of contaminants and favors their degradation in the freshwater part of the estuary. Results of the additional monitoring of two tidal streams located downstream of Arzal dam suggested that, although some compounds may be transferred to the estuary, their impact was probably very low. Dilution by marine water associated with tidal current was also a major factor of concentration reduction. It is concluded that the highest risks associated to herbicides and booster biocides concerned the freshwater part of the estuary and that its brackish/saltwater part was exposed to a moderate risk, although some substances may sometimes exhibit high concentration but mainly at low tide and on an irregular basis.
Afficher plus [+] Moins [-]Characterization of endocrine disruptors from a complex matrix using estrogen receptor affinity columns and high performance liquid chromatography-high resolution mass spectrometry Texte intégral
2013
Jondeau-Cabaton, Adeline | Soucasse, Amélie | Jamin, Emilien L. | Creusot, Nicolas | Grimaldi, Marina | Jouanin, Isabelle | Ait-Aissa, Selim | Balaguer, Patrick | Debrauwer, Laurent | Zalko, Daniel | ToxAlim (ToxAlim) ; Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | MetaToul AXIOM (E20) ; ToxAlim (ToxAlim) ; Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-MetaboHUB-MetaToul ; MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institut National de l'Environnement Industriel et des Risques (INERIS) | Laboratoire de Physico et Toxico-Chimie des systèmes naturels (LPTC) ; Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS) | Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1) ; Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM) | Métabolisme et Xénobiotiques (ToxAlim-MeX) ; ToxAlim (ToxAlim) ; Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | "Ministere de l'Environnement, du Developpement Durable et de la Mer" (MEDDM), "ECOPI" project [P189]
Characterization of endocrine disruptors from a complex matrix using estrogen receptor affinity columns and high performance liquid chromatography-high resolution mass spectrometry Texte intégral
2013
Jondeau-Cabaton, Adeline | Soucasse, Amélie | Jamin, Emilien L. | Creusot, Nicolas | Grimaldi, Marina | Jouanin, Isabelle | Ait-Aissa, Selim | Balaguer, Patrick | Debrauwer, Laurent | Zalko, Daniel | ToxAlim (ToxAlim) ; Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | MetaToul AXIOM (E20) ; ToxAlim (ToxAlim) ; Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-MetaboHUB-MetaToul ; MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institut National de l'Environnement Industriel et des Risques (INERIS) | Laboratoire de Physico et Toxico-Chimie des systèmes naturels (LPTC) ; Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS) | Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1) ; Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM) | Métabolisme et Xénobiotiques (ToxAlim-MeX) ; ToxAlim (ToxAlim) ; Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | "Ministere de l'Environnement, du Developpement Durable et de la Mer" (MEDDM), "ECOPI" project [P189]
International audience | Complex mixtures of contaminants with potential adverse effects on human health and wildlife are found in the environment and in the food chain. These mixtures include numerous anthropogenic compounds of various origins and structures, which may behave as endocrine disruptors. Mixture's complexity is further enhanced by biotic and abiotic transformations. It is therefore necessary to develop new strategies allowing the identification of the structure of known, as well as unknown, nuclear receptor (NR) ligands present in complex matrices. We explored the possibility to use NR-based affinity columns to characterize the presence of bioactive molecules in environmental complex mixtures. Estrogen receptor alpha (ERalpha)-based affinity columns were used to trap and purify estrogenic substances present in surface sediment samples collected in a French river under mixed anthropogenic pressure. We combined biological, biochemical and analytical approaches to characterize the structure of ligands retained on columns and demonstrate the presence of known active molecules such as bisphenol A and octylphenol, but also of unexpected ERalpha ligands (n-butylparaben, hydroxyl-methyl-benzofuranone). High resolution mass spectrometry results demonstrate that ERalpha affinity columns can be used for the isolation, purification and identification of known as well as unknown estrogenic contaminants present in complex matrices.
Afficher plus [+] Moins [-]Characterization of endocrine disruptors from a complex matrix using estrogen receptor affinity columns and high performance liquid chromatography–high resolution mass spectrometry Texte intégral
2013
Jondeau-Cabaton, Adeline | Soucasse, Amélie | Jamin, Emilien L. | Creusot, Nicolas | Grimaldi, Marina | Jouanin, Isabelle | Aït-Aïssa, Sélim | Balaguer, Patrick | Debrauwer, Laurent | Zalko, Daniel
Complex mixtures of contaminants with potential adverse effects on human health and wildlife are found in the environment and in the food chain. These mixtures include numerous anthropogenic compounds of various origins and structures, which may behave as endocrine disruptors. Mixture’s complexity is further enhanced by biotic and abiotic transformations. It is therefore necessary to develop new strategies allowing the identification of the structure of known, as well as unknown, nuclear receptor (NR) ligands present in complex matrices. We explored the possibility to use NR-based affinity columns to characterize the presence of bioactive molecules in environmental complex mixtures. Estrogen receptor α (ERα)-based affinity columns were used to trap and purify estrogenic substances present in surface sediment samples collected in a French river under mixed anthropogenic pressure. We combined biological, biochemical and analytical approaches to characterize the structure of ligands retained on columns and demonstrate the presence of known active molecules such as bisphenol A and octylphenol, but also of unexpected ERα ligands (n-butylparaben, hydroxyl-methyl-benzofuranone). High resolution mass spectrometry results demonstrate that ERα affinity columns can be used for the isolation, purification and identification of known as well as unknown estrogenic contaminants present in complex matrices.
Afficher plus [+] Moins [-]