Heavy metal pollution is a global ecological safety issue, especially in crops, where it directly threatens regional ecological security and human health. Selecting scientific evaluation methods is an important prerequisite for understanding the distribution of heavy metals in a region. To evaluate the distribution characteristics of arsenic (As), cadmium (Cd), copper (Cu), and zinc (Zn) in farmland soil–rice system in Doumen District of Zhuhai City, Pearl River Delta, we analyzed the high geological background area and heavy metal contents in soil by inverse distance–weighted interpolation and single-factor pollution index. Bioconcentration factor (BCF) was used to study the migration and accumulation characteristics of heavy metals. Then, the soil–rice system was evaluated comprehensively with a novel evaluation method, i.e., the influence index of comprehensive quality (IICQ). Results showed that As, Cd, Cu, and Zn in the soil of the study area followed normal distribution. Cd and Cu were the main pollutants whose point contamination rates were 50% and 22.86%, respectively. A total of 2.86% of the soil were contaminated by As, and no Zn contamination was observed in the soil. At the same time, As and Cd in rice were partially polluted, and the Cu and Zn were not polluted. The order of bioaccumulation coefficient was Cd > Zn > Cu > As, and no evident enrichment was observed. According to the impact index of IICQ to evaluate the pollution of heavy metals in the soil–rice system, 96.98% of the soil in study area was in a state of moderate, heavy, and extreme pollution, which were concentrated in the northern and central parts of the study area. The soil–rice system in the high geological background area was in a subhealthy state. A total of 90.69% of the soil were polluted, but the rice met the national food safety standards.

This study investigates the asymmetric effects of economic growth, energy use, and financial development, on carbon dioxide emissions in Saudi Arabia, from 1971 to 2014, using the nonlinear autoregressive distributed lag (NARDL) model. Prior to the application of the model, the integration proprieties of the variables were examined employing the recently RALS-LM (Residual Augmented Least squares—Lagrange Multiplier) unit root test, with two endogenous structural breaks. The main finding is that there exists an asymmetric cointegration relationship among the variables. In the long-run, both positive and negative shocks in economic growth rise emissions, but the effect of positive shocks is larger. In addition, both positive shocks in energy consumption and negative shocks in financial development surge CO₂ emissions. In the short-run, the increasing economic growth is being made at the expense of the polluted environment. In contrast, any decrease in the economic growth would contribute to the improvement of environmental quality. Furthermore, positive shocks on energy consumption surges CO₂ emissions and positive shocks in financial development reduces emissions. The asymmetric causality test of Hatemi-J (2012) suggests that economic growth (positive shocks) causes carbon dioxide emissions. At the same time, CO₂ emissions (positive shocks) cause energy consumption. However, no significant causal relationship is found between financial development and CO₂ emissions. In light of these findings, some policy implications are recommended.

Starch was transformed to hydrophobic starch phthalate (contact angle 109°) in order to achieve a good dispersion in LDPE matrix. Nanosilica derived from rice husk after aminopropyltrimethoxysilane functionalization was also incorporated into the blend as property-enhancing filler. The produced crystalline starch phthalate had a lower particle size of 9.87 μm and a higher surface area of 2.87 m²/g compared to starch (40.28 μm, 1.91 m²/g). The potential quality modification of starch phthalate as a substitute for starch towards the production of a perfect biodegradable blend was quantified in terms of mechanical (tensile, tear, stiffness), optical (haze, transmittance), and biodegradation assessments. Interfacial adhesion between LDPE and starch phthalate was well justified by the morphology and enhancement in mechanical properties like tensile and tear strength from 8.87 to 12.67 MPa and 96.57 to 187.10 N/mm for 30% of starch or starch phthalate in LDPE matrix, respectively. Starch phthalate compared to starch blended films showed a higher biodegradation rate of 14.8 and 13.5% in garden soil and vegetable waste respectively in 1 year (at 30% biofiller), with a good first-order kinetics fit of the weight loss data having a higher degradation rate constant at higher content of biofiller in the blend.

Remediation of heavy metal and polycyclic aromatic hydrocarbon (PAH)–co-contaminated soils has drawn much more attention; phytoremediation is an often-used technique. Sudan grass (Sorghum sudanense (Piper) Stapf.) with developed root system and strong PAHs and heavy metal tolerance is a potential choice for phytoremediation. In this study, the application of tea saponin (TS) (1 g kg⁻¹ soil) and nitrilotriacetic acid (NTA) (1 g kg⁻¹ soil) was to improve the removal efficiency of Ni and pyrene. TS and NTA had no obvious effects on the growth and soluble proteins of Sudan grass. Ni concentration in root was higher than that in the shoot. The addition of TS and NTA increased the Ni concentration in the root by 25.98% in Ni-contaminated treatment. Pyrene was mainly accumulated in the shoot of Sudan grass. Pyrene concentration in shoot increased by 20.14% with TS-NTA in pyrene-contaminated treatment and increased by 31.97% in Ni-contaminated treatment. TS and NTA had significantly improved dissolved organic matter and soil microbial activity. Microbial activity increased by 16.75%, 18.07%, and 23.364% in pyrene-contaminated, Ni-contaminated, and pyrene and Ni–co-contaminated treatment, respectively. This study showed that phytoremediation of pyrene and Ni–co-contaminated soil by Sudan grass could be enhanced by the application of TS-NTA and the interaction between pyrene and Ni impacted the accumulation of Ni and pyrene in Sudan grass.

Keratin-based adsorbents showed a great potential in environmental pollution remediation. However, fabrication of keratin adsorbent with an excellent performance remains a challenging issue mainly because of its poor mechanical properties. In this research, mechanical-reinforced keratin adsorbent was designed and engineered at mesoscopic scale by the induced molecular network transition method. It was found that the β-crystallite structure of silk fibroin template could induce the transformation of free unfolded molecular chains of keratin to β-sheet conformation in the keratin adsorbent and further resulted in the controllable manipulation of the mechanical properties of the adsorbent. The prepared keratin adsorbent exhibited an excellent adsorption performance for Reactive Black 5 (RB5). The qₑ and removal efficiency for RB5 by the adsorbent could reach as high as 550 mg/g and 95.3%, respectively. The adsorbent exhibited an excellent regeneration and recycle performance due to its mechanical reinforcement. The facile molecular network–induced reconstruction strategy is both straightforward and effective for fabricating mechanical robust adsorbent for environmentally pollutant remediation. Graphical abstract

Tuta absoluta Meyrick originates in South America and is now one of the most important insect pests of Solanaceae in different parts of the world, including Africa. Its control has relied primarily on chemical insecticides, which are associated with negative ecological effects. In the present study, essential oils of Ocimum gratissimum and O. kilimandscharicum were tested for repellence and fumigant toxicity on the adult stages under laboratory conditions. The oil of O. gratissimum was more repellent, but its toxicity was comparable with that of O. kilimandscharicum. The major constituents of O. gratissimum were methyl eugenol (39.5%) and eugenol (29.7%). Those of O. kilimandscharicum were camphor (47.1%) and 1.8-cineole (19.3%). Eugenol (LC₅₀ of 0.24 μl/ml, 83.3%, RI₅₀ = 0.15) and camphor (LC₅₀ of 0.23 μl/ml, 89.5%, RI₅₀ = 0.13) were more toxic (at 1 μl/ml for 24 h) and repellent than the other constituents. The results show potential of the essential oils for use in integrated management of the tomato pest.

Ammonia oxidation is mainly performed by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). Allylthiourea (ATU) has been found to specifically inhibit ammonia oxidation. However, the effect of ATU on AOA and AOB transcription has been infrequently studied. In the present study, we examined the responses of AOA and AOB activity and DNA/cDNA community structure to ATU exposure. The ammonia oxidation activity in the 100-mg/L ATU group was 4.3% of that in the control group after 7 days. When exposed to ATU, the gene abundance of AOA was favored compared with that of AOB, and there were no statistically significant differences in the abundance of AOB amoA in DNA and cDNA between the two groups. Compared with the control group, the gene abundance of AOA significantly increased by 5.23 times, while the transcription of AOA significantly decreased by 0.70 times. Moreover, the transcriptional ratio of AOA in the ATU group was only 0.05 times as high as that in the control group. ATU selectively affected AOB and completely inhibited Nitrosomonas europaea and Bacterium amoA.22.HaldeII.kultur at the genetic level. Under ATU exposure, all AOA clusters were transcribed, but three AOB clusters were not transcribed. Our results indicated that the ammonia oxidation potential of the soil of water level fluctuation areas, based on ATU inhibition, was associated mainly with AOA amoA gene abundance and AOB community shifts in DNA and cDNA.

In this study, the possibility of culturing Dunaliella salina in stickwater (SW) as the main effluent of fishmeal plants was evaluated. D. salina was grown in different media obtained by replacing standard Guillard medium (F/2) with SW at 0% (control), 10%, 25%, 50%, 75%, and 100% ratios. The cell density, pigment contents, proximate composition, saponification value, and fatty acids (FAs) profiles were measured for 14 days. SW was collected from a kilka fishmeal factory in northern Iran, and the characteristics indicated high concentrations of nitrate (242.00 mg L⁻¹) and phosphate (11.13 mg L⁻¹). A significant increase in the cell density was observed in 14 days when 75% SW was used. Moreover, SW significantly affected the pigment contents. The highest contents of chlorophylls, total carotenoids, and β-carotene (3.64 μg mL⁻¹) were calculated in 75% SW. According to the algal proximate composition, the highest and lowest contents of lipid were accumulated in 75% and 100% SW, respectively (p < 0.05). The highest level of saturated FAs was observed in 75% SW compared with the others (p < 0.05). In conclusion, replacing F/2 with SW indicated the capability of D. salina to grow in a treated medium with 75% SW substitution as a bioremediator.

The emerging pollutants cover a wide range of synthetic chemicals that are indispensable to modern society but with little known effects for aquatic animals and for people who consume polluted waters with these products. Gels manipulated with hormones are widely used for hormone replacement, muscle growth, among other purposes. However, only a small part of these hormones are absorbed into the skin, and so these can be transferred to the domestic sewage during the washing of exposed body regions. Thus, the aim of this study was to evaluate the toxicity levels for the bioindicator Artemia salina L., and cytotoxicity and mutagenicity for the bioindicator Allium cepa L. of gels handled with 1% testosterone and 0.1% estradiol. Data from immobile/dead A. salina numbers after 24 h of exposure showed that the highest concentrations of testosterone (10 and 25 μg/mL) and the highest concentration of estradiol (15 μg/mL) were toxic to this bioindicator. For the bioindicator A. cepa, mitotic indices and chromosomal aberrations did not indicate statistical differences between the groups treated with the testosterone gels (1, 10, 50 μg/mL) and estradiol (0.03, 0.30, 1.5 μg/mL) and the control group. However, all concentrations of the testosterone-containing gel decreased the percentage of cell division in relation to the time 0 h of each treatment and to the time 24 h of the negative control. Therefore, it is concluded that rivers or aquatic environments can be polluted if wastewater with the toxic concentrations found of these hormonal gels is discarded without previous treatment, compromising the life of organisms that live there. And, it encourages the development of techniques for treating sewage and water to reduce/eliminate the hormones present in them.

Using organic insecticides including plant oils, it is possible to design a new perspective for the control of insect pests. In this research, nanoemulsion formulations of Mentha piperita, wild-type essential oil (EO) were prepared utilizing high-energy ultrasonication process. Physicochemical properties of nanoemulsions were precisely studied by measurement various parameters including pH, viscosity, conductivity, and zeta potential. Experimental design by the aid of response surface methodology (RSM) was used to highlight the physicochemical roles of EO percentage (1% to 5% (v/v)) and surfactant concentration (3% to 15% (v/v)) for achieving minimum droplet diameter with high physical stability. The nanoemulsion formulations were then characterized using dynamic light scattering, transmission electron microscopy, and optical clarity. Afterward, an appropriate model between the variable factors (EO percentage and surfactant concentration) and the response (hydrodynamic particle size) was statistically developed. Under the optimum conditions, nanoemulsion with hydrodynamic particle size less than 10 nm with high physical stability is obtainable. Bioassay experiments were carried out to elucidate the effects of nanoemulsion on the cotton aphid. Synthesized nanoemulsion formulations showed relatively high contact toxicity (average value of LC₅₀ was about 3879.5 ± 16.2 μl a.i./L) against the pest. On the basis of the obtained results, prepared nanoemulsion using M. piperita is potentially applicable as organic insecticides against cotton aphid. Graphical abstract