Many complaints have been made about bad smelling and tasting food and accidents resulting in the death of animals in Nagano Prefecture. The method of headspace SPME-GC/MS has been found very effective as a screening method to investigate the cause of these complaints and accidents. This method is characterized mainly in that the operation is simple without using any solvents for extraction, causing no environmental pollution; the extraction may be carried out in a short time with a small quantity of sample in low concentration, preventing reagent contamination, the whole extract may be injected, leading to high sensitivity, and the fiber may be used repeatedly, making the analytical cost inexpensive. This paper describes representative examples of cases in which the relevant causes have been solved.

An investigation was made to find analytical method for anilazine and propazine remaining in agricultural products. Since anilazine was found to be decomposed enzymatically when applied on cabbage, radish, etc., phosphoric acid was added to the sample to suppress the enzymatic activity before subjecting the sample to an extraction with acetone. The extract was re-extracted into n-hexane, cleaned up with a Florisil column, and finally subjected to a determination with ECD-GC. Propazine was found to be insusceptable to any enzymatic decomposition, but it was subjected to the same acetone extraction and n-hexane re-extraction as with anilazine, separated from anilazine with a Florisil column, and subjected to a determination with NPD-GC. The detection limits of these methods are 0.01 micro g/g for both anilazine and propazine. The addition-recovery experiments conducted resulted in recoveries of over 79 and 82% with coefficient of variation of less than 5.6 and 5.5% for alilazine and propazine, respectively (n=5). The present methods were applied to actual agricultural products, with neither anilazine nor propazine detected (n=22).

Management of aquatic ecosystems and planning of their protection needs good knowledge about their conditions. Mathematical modeling becomes one of the basic things in that processes. Last decades was attain important progress in quality at the beginning relative modest models of aquatic ecosystems. During the analysis of existing experiences to forced a conclusion that a quality model in one's own structures needs proportional representation of hydrodinamic and ecology background.

The sampling of Crnojevica river has been carried out, because of estimation the correlation between main water quality parameters and precipitation impacts, as well as adequate hydrological parameters. An appropriate dynamic sampling has been applied. Continuous pollution of river water has been confirmed, as well as source of the pollution. It is estimated strong correlation of water quality parameters from precipitation quantity and water level, especially for sanitary pollution. Change of quality parameters value is discrete and accidental.

In recent works enzymatic phosphatase activity state as a new potential good indicator for water saprobity and trophic state in water. On water reservoir 'Grliste' reinstated monitoring on the base physico-chemical, microbiological and biological analysis from beginning works water factory 1990 but, from 1998 use of this new biochemical method in monitoring as reliable additional indicator in fast preliminary water quality and the ecophysiological state of water. According to the results obtained for index of phosphatase activity (IFA) (Matavulj, 1986) water of 'Grliste' water accumulation belongs to category 3-A (polluted water).