To assess genetoxicity and the underlying mechanisms of carbamazepine (CBZ) toxicity in fish, adult Chinese rare minnows (Gobiocypris rarus) were exposed to 1, 10, and 100 μg/L CBZ for 28 d. Comet assays indicated that hepatic DNA damage was significantly increased in groups of minnows exposed to CBZ at all concentrations in a dose-dependent manner compared to those of the control groups (p < 0.05). Liver levels of 8-hydroxydeoxyguanosine (8-OHdG) were significantly increased at 10 and 100 μg/L CBZ (p < 0.05). TUNEL assays indicated that the average apoptotic rates of the livers of female and male minnows were significantly increased following exposure to CBZ at all concentrations for 28 d (p < 0.05). Significant increases in caspase 3 and 9 activities after CBZ exposure at all concentrations and caspase 8 at 10 and 100 μg/L CBZ exposure reflected the presence of mitochondrial apoptosis (p < 0.05). The mRNA levels of gadd45a, mdm2, casp3 and casp9 in female and male minnows exposed to CBZ at all concentrations were significantly increased compared with those in the control groups (p < 0.05). Significant increases in the levels of p21 in female minnows exposed to 1 and 100 μg/L CBZ, p53 in female minnows at all CBZ treatments and bcl2 in male minnows exposed to 1 and 100 μg/L CBZ were observed, indicating p53 pathway activation. The inhibition of ras levels in females and males exposed to CBZ at all concentrations and increased levels of raf1 in males exposed to CBZ at all concentrations indicated Ras/Raf1/MAPK (ERK) activation. Therefore, the present study demonstrates that CBZ at environmentally relevant levels induces DNA damage and apoptosis in Chinese rare minnows by the Ras/Raf/ERK/p53 signaling pathway.

Excessive nutrient discharges have resulted in pervasive water pollution and aquatic eutrophication. China has made massive efforts to improve water quality since 2000. However, how long-term policy interventions govern external and internal fluxes as well as nitrogen (N) concentrations is not well known. Here we examined the historical N concentration change and its key drivers in eutrophic Lake Dianchi (southwest China) over the period 2002–2018, based on monthly observations of water quality and external N fluxes, local surveys of mitigation measures, and process-based model simulations of internal N fluxes. Our data indicated that N concentrations peaked at 3.0 mg L⁻¹ in 2007–2010 but afterwards declined down to 1.2 mg L⁻¹ in 2018. Compared with 2010, the decline in lake N concentrations was attributed to reduced riverine N inflow decreasing by 0.20 g N m⁻³ month⁻¹ and the water-sediment exchange flux decreasing by 0.07 g N m⁻³ month⁻¹ from 2010 to 2018. Adoptions of wastewater treatment, pollution interception, and transboundary water transfer dominated the changes in external and internal fluxes of N and thereby the decline of lake N concentrations. These findings underscore the priority of reducing external discharge for historical lake water quality improvement and the need of enhancing internal N removal for future lake ecosystem restoration.

Heavy metal contamination has been threatening the health of human beings. To decrease the bio-toxicity of heavy metals, a thiol-functionalized nano-silica (SiO₂-SH) was adopted to remediate the soil contaminated by lead (Pb), cadmium (Cd) and copper (Cu). The remediation effect of SiO₂-SH on contaminated soils was investigated by the uptake of the heavy metals into lettuce and pakchoi in pot experiment. The bio-toxicity of the SiO₂-SH was evaluated, and its immobilization mechanisms were proposed by the fraction distribution of Cd, Pb and Cu. It was found that the SiO₂-SH can significantly reduce the uptake of Cd, Pb, Cu into pakchoi by 92.02%, 68.03%, 76.34% and into lettuce by 89.81%, 43.41%, 5.76%, respectively. The chemical species analyses of Cd, Pb, Cu indicate SiO₂-SH can transform the heavy metal in acid soluble states into reducible fraction and oxidizable fraction, thereby inhibiting the extraction of heavy metals into soil solution. The concentrations of microbial biomass carbon, organic matter, and cation exchange capacity of the soil increased while the soil bulk density decreased after remediation. Those changes demonstrate that SiO₂-SH not only has no bio-toxic impact on the soil environment but also improves the soil environment, which proves the prepared SiO₂-SH is environmental-friendly. The SiO₂-SH could be a promising amendment for heavy metal contaminated soils.

Microplastics (MPs) are becoming an environmental growing concern, being the sewage sludge applied to agriculture fields one of the most important inputs to the environment. To date, there is no standardized protocol for their extraction and changes in vegetative growth and fruit maturation on cultivated plants induced by sludge containing MPs have not been studied yet. Sewage sludge from three different wastewater treatment plants located in Murcia, Spain, were studied. First, the microplastic concentration was estimated and, then, the effects of the sewage sludge in the development of tomato plants and fruit production was analyzed. The measured parameters in tomato plants were both, biomass and length, for shoot and root part, as well as, stem diameter and tomato production. The present work has developed and validated a protocol for the extraction and quantification of MPs comprising several shapes, materials and sizes from samples of sewage sludges, which offers a good compromise for the extraction of different types of microplastic. The protocol used for MPs extraction had a recovery efficiency of 80 ± 3% (mean ± SE) and used bicarbonate, to maximize MPs extraction. The mean abundance of MPs in the studied sewage sludge samples was 30,940 ± 8589 particles kg⁻¹ dry weight. Soils with sludge containing MPs fostered the growth of tomato plants, while delaying and diminished fruit production. However, other factors or their interactions with MPs could have influenced the outcomes. Further studies are necessary to corroborate these findings and explain the mechanisms of possible effects of MPs on plants.

Antibiotic proliferation in the environment and their persistent nature is an issue of global concern as they induce antibiotic resistance threatening both human health and the ecosystem. Antibiotics have therefore been categorized as emerging pollutants. Fluoroquinolone (FQs) antibiotics are an emerging class of contaminants that are used extensively in human and veterinary medicine. The recalcitrant nature of fluoroquinolones has led to their presence in wastewater, effluents and water bodies. Even at a low concentration, FQs can stimulate antibacterial resistance. The main sources of FQ contamination include waste from pharmaceutical manufacturing industries, hospitals and households that ultimately reaches the wastewater treatment plants (WWTPs). The conventional WWTPs are unable to completely remove FQs due to their chemical stability. Therefore, the development and implementation of more efficient, economical, convenient treatment and removal technologies are needed to adequately address the issue. This review provides an overview of the technologies available for the removal of fluoroquinolone antibiotics from wastewater including adsorptive removal, advanced oxidation processes, removal using non-carbon based nanomaterials, microbial degradation and enzymatic degradation. Each treatment technology is discussed on its merits and limitations and a comparative view is presented on the choice of an advanced treatment process for future studies and implementation. A discussion on the commercialization potential and eco-friendliness of each technology is also included in the review. The importance of metabolite identification and their residual toxicity determination has been emphasized. The last section of the review provides an overview of the policy interventions and regulatory frameworks that aid in retrofitting antibiotics as a central key focus contaminant and thereby defining the discharge limits for antibiotics and establishing safe manufacturing practices.

Pollution of riverine ecosystems through the multidimensional impact of human footprints around the world poses a serious challenge. Research studies that communicate potential repercussions of landscape structure metrics on snowmelt riverine water quality particularly, in climatically fragile Himalayan watersheds are very scarce. Though, worldwide, grasping the influence of land-use practices on water quality (WQ) has received renewed attention yet, the relevance of spatial scale linked to landscape pattern is still elusive due to its heterogenic nature across diverse geomorphic regions. In this work, therefore, we tried to capture the insights on landscape-aquascape interface by juxtapositioning the impacts of landscape structure pattern on snowmelt stream WQ of the whole Jhelum River Basin (JRB) under three varying spatial scales viz., watershed scale, riparian corridor (1000 m wide) and reach buffer (500 m wide). The percentage of landscape pattern composition and configuration metrics in the JRB were computed in GIS utilizing Landsat-8 OLI/TIRS satellite image having 30 m resolution. To better explicate the influence of land-use metrics on riverine WQ with space and time, we used Redundancy analysis (RDA) and multilinear regression (MLR) modeling. MLR selected land-use structure metrics revealed the varied response of WQ parameters to multi-scale factors except for total faecal coliform bacteria (TC) which showed perpetual presence. The reach-scale explained slightly better (76%) variations in WQ than riparian (75%) and watershed (70%) scales. Likewise, across seasonal scale, autumn (75%), winter (83%), and summer (77%) captured the most WQ variation at catchment, riparian, and reach scales respectively. We observed impairing WQ linkages with agriculture, built-up and barren rocky areas across watersheds, besides, pastures in riparian buffer areas, and fragmentation of landscape patches at the reach scale. Due to little appearance of spatial scale differences, a multi scale perspective landscape planning is emphasized to ensure future sustainability of Kashmir Himalayan water resources.

Studies have shown that the mixture toxicity of compounds with similar modes of action (MOAs) is usually predicted by the concentration addition (CA) model. However, due to the lack of toxicological information on compounds, more evidence is needed to determine whether the above conclusion is generally applicable. In general, the same type of compounds with similar chemical structures have similar MOAs, so whether the toxicities of the mixture of these compounds are additive needs to be further studied. In this paper, three types of pesticides with similar chemical structures (three organophosphoruses, two carbamates and two neonicotinoids) that may have similar MOAs were selected and five binary mixture systems were constructed. For each system, five mixture rays with different concentration ratios were designed by the direct equipartition ray design (EquRay) method. The mortality of Caenorhabditis elegans was regarded as the endpoint for the toxicity exposure to single pesticides and binary mixtures. The combined toxicities were evaluated simultaneously using the CA model, isobologram and combination index. The structural similarity of the same type of pesticides was quantitatively analyzed according to the MACCS molecular fingerprint and the slope of dose-response curve at pEC₅₀. The results show that the toxicities of neonicotinoid mixtures and carbamate mixtures are almost antagonistic. The entire mixture system of dichlorvos and dimethoate produced synergism, and four of the five mixture rays of dimethoate and methamidophos induced antagonism, while among the mixture rays of dichlorvos and methamidophos, different concentrations showed different interaction types. The results of structural similarity analysis show that the size of structural similarity showed a certain quantitative relationship with the toxicity interaction of mixtures, that is, the structural similarity of the same type of pesticides may show an additive action in a certain range.

Enhancing and investigating the photocatalytic activity over composites for new models remains a challenge. Here, an emerging S-scheme photocatalyst composed of 2D/0D g-C₃N₄ nanosheets-assisted SnO₂ nanoparticles (g-C₃N₄/SnO₂) is successfully synthesized and used for degrading nitrogen oxide (NO), which causes negative impacts on the environment. A wide range of characterization techniques confirms the successful synthesis of SnO₂ nanoparticles, g-C₃N₄ nanosheets, and 2D/0D g-C₃N₄/SnO₂ S-scheme photocatalysts via hydrothermal and annealing processes. Besides, the visible-light response is confirmed by optical analysis. The S-scheme charge transfer was elucidated by Density-Functional Theory (DFT) calculation, trapping experiments, and electron spin resonance (ESR). We found that intrinsic oxygen vacancies of SnO₂ nanoparticles and S-scheme charge transfer addressed the limitation of other heterojunction types. It is notable that compared pure SnO₂ nanoparticles and g-C₃N₄, g-C₃N₄/SnO₂ offered the best photocatalytic NO degradation and photostability under visible light with the removal of more than 40% NO at 500 ppb throughout the experiment. Benefiting from the unique structural features, the new generation architectural structure of S-scheme heterojunction exhibited potential photocatalytic activity and it would simultaneously act more promising for environmental treatment in the coming years.

We investigated the concentration, composition, and potential risk of organophosphate esters (OPEs) and phthalates (PAEs) in soils and vegetation from rural areas of Dalian, Northeast China. The residues of total OPEs and PAEs in soils were in the range of 33.1–136 ng/g dw (dry weight) and 465–5450 ng/g dw, while the values in plants were 140–2360 ng/g dw and 2440–21800 ng/g dw, respectively. The concentrations of both chemicals in the plant rhizosphere soils were significantly lower than those in the bulk soils, suggesting an enhanced degradation or uptake by plant. The contaminations in soils also varied for different land use types with the concentrations generally higher in paddy soils than those in maize soils. The OPE and PAE concentrations in plant leaves were slightly higher than those in their corresponding roots. The bioconcentration factors of OPEs & PAEs were significantly negatively correlated with their octanol-water partition coefficients. A hazard assessment suggested potential medium to high risks from tricresyl phosphate (TMPP) and di-n-butyl phthalate (DNBP) for the agricultural soils in Dalian of China. Although the ecological risks of OPEs and PAEs in the rhizosphere soils were lower than those in the bulk soils, the relevant risk could still endanger human health via oral intake of these plants. The daily dietary intakes of OPEs and PAEs via vegetable and rice consuming were estimated, and the result suggests a higher exposure risk via ingestion of leafy vegetable than rice.

With the implementation of clean air strategies, PM₂.₅ pollution abatement has been observed in the “2 + 26” cities in the Beijing-Tianjin-Hebei (BTH) region (referred to as the BTH2+26) and their surrounding areas. To identify the drivers for PM₂.₅ concentration decreases in the BTH2+26 cites from the 2016/17 heating season (HS1617) to the 2017/18 heating season (HS1718), we investigated the contributions of meteorological conditions and emission-reduction measures by Community Multi-Scale Air Quality (CMAQ) model simulations. The source apportionments of five sector sources (i.e., agriculture, industry, power plants, traffic and residential), and regional sources (i.e., local, within-BTH: other cities within the BTH2+26 cities, outside-BTH, and boundary conditions (BCON)) to the PM₂.₅ decreases in the BTH2+26 cities were estimated with the Integrated Source Apportionment Method (ISAM). Mean PM₂.₅ concentrations in the BTH2+26 cities substantially decreased from 77.4 to 152.5 μg m⁻³ in HS1617 to 52.9–101.9 μg m⁻³ in HS1718, with the numbers of heavy haze (daily PM₂.₅ ≥150 μg m⁻³) days decreasing from 17-77 to 5–30 days. The model simulation results indicated that the PM₂.₅ concentration decreases in most of the BTH2+26 cities were attributed to emission reductions (0.4–55.0 μg m⁻³, 2.3–81.6% of total), but the favorable meteorological conditions also played important roles (1.9–25.4 μg m⁻³, 18.4–97.7%). Residential sources dominated the PM₂.₅ reductions, leading to decreases in average PM₂.₅ concentrations by more than 30 μg m⁻³ in severely polluted cities (i.e., Shijiazhuang, Baoding, Xingtai, and Beijing). Regional source analyses showed that both local and within-BTH sources were significant contributors to PM₂.₅ concentrations for most cities. Emission controls in local and within-BTH sources in HS1718 decreased the average PM₂.₅ concentrations by 0.1–47.2 μg m⁻³ and 0.3–22.1 μg m⁻³, respectively, relative to those in HS1617. Here we demonstrate that a combination of favorable meteorological conditions and anthropogenic emission reductions contributed to the improvement of air quality from HS1617 to HS1718 in the BTH2+26 cities.