Median enterococci counts of beach water samples gradually increased at statistically significant levels (χ2: 26.53, df: 4; p<0.0001) with increasing proximity to river influx. The difference in proportion of antibiotic resistant enterococci in beach water and river water samples was statistically significant (p<0.05) for the tested antibiotics with river isolates generally presenting higher resistance frequencies. Virulence genes cyl, esp, gelE and asa were detected at varying frequencies (7.32%, 21.95%, 100% and 63.41% respectively) among river isolates. On the other hand, the prevalence of these genes was lower (0%, 20%, 67.27% and 41.82% respectively) among beach water isolates. Multi-Locus-Sequence-Typing analysis of Enterococcusfaecalis presented four sequence types (ST) one of which shared six out of seven tested loci with ST6, a member of the clonal complex of multi-drug resistant strains associated with hospital outbreaks.

Total dissolved copper (Cu) and Cu speciation were examined from inshore waters of Bermuda, in October 2009 and July–August 2010, to determine the relationship between total dissolved Cu, Cu-binding ligands and bioavailable, free, hydrated Cu2+ concentrations. Speciation was performed using competitive ligand exchange-adsorptive cathodic stripping voltammetry (CLE-ACSV). Mean total dissolved Cu concentrations ranged from 1.4nM to 19.2nM, with lowest concentrations at sites further from shore, consistent with previous measurements in the Sargasso Sea, and localized Cu enrichment inshore in enclosed harbors. Ligand concentrations exceeded dissolved [Cu] at most sites, and [Cu2+] were correspondingly low at those sites, typically <10−13 M. One site, Hamilton Harbour, was found to have [Cu] in excess of ligands, resulting in [Cu2+] of 10−10.7 M, and indicating that Cu may be toxic to phytoplankton here.

The distributions of 41 polychlorinated biphenyls (PCBs) were determined in the aqueous phase, suspended particulate matter (SPM), and sediment of the Daliao River estuary in Liaodong Bay, Bohai Sea (China). The total PCB concentrations ranged from 5.51 to 40.28ngL−1 in the surface water, from 6.78 to 66.55ngL−1 dry weight in the SPM, and from 0.83 to 7.29ngg−1 dry weight in the sediment. The PCB concentrations in water, SPM, and sediment were moderate relative to those reported for other estuary and marine systems around the world. Sedimentary PCB concentrations decreased offshore due to the active deposition of laterally transported river-borne particles. The predominance of the highly chlorinated congeners for the water, SPM, and sediment samples are an indication of either a lack of degradation or the presence of nearby or recent releases into the environment.

The effect of firework displays on ambient particulate pollution in a typical valley city in Northwestern China was evaluated based on high temporal resolution atmospheric particle size distribution (10–10 000nm) data and particle mass concentrations in different sizes obtained during 25th January – 24th February, 2013. Firework displays have significant impact on particle number concentrations in accumulation mode (100–1 000nm), especially in 200–500nm, as well as PM1 mass concentrations. The hourly mean number concentration in 200–500 nm and PM1 mass concentration during the peak hour of firework displays were 11 800.2±2 548.0cm–3 and 214.1±31.2μg m−3, which are approximately 6 times and 2 times of that before the festival, respectively, with a maximum10–min mean number concentration in size range 100–1 000 nm reaching 3.8×104cm−3 on the New Year's Eve (00:10 BT 10th February, 2013). It was estimated that local emissions and firework displays contributed 74.6% and 37.0%, respectively, to the number concentration of particles less than 1 000nm. On short–time scale, the contribution of firework displays on local particulate pollution is obvious and should not be ignored, especially on fine particulate pollution.

2D URANS CFD simulations were conducted to study the effect of short–time variations of wind velocity on mass transfer rate between street canyons and the atmospheric boundary layer (ABL). A street canyon with a height–to– width ratio (aspect ratio) of three was considered as a case study. The study is of practical interest since it illustrates a skimming flow regime, the regime where pollutants are less effectively exchanged between the canyon and the above atmosphere, typically found in many urban areas in Mediterranean countries. Short–time variations of wind velocity magnitude were simulated assuming a sinusoidal function with average magnitude = 4m s−1; amplitude ±2m s−1 and period from 1 to 40 s, and subsequently with short–time averaged (0.1 s, 1 s and 10 s) real world data measured with an ultrasonic anemometer (50Hz). Mass transfer rate between the canyon and the ABL was evaluated as the rate of reduction of spatially averaged concentration of a passive pollutant, carbon monoxide (CO), in the street canyon. Results show that mass transfer rate increases with the frequency of short–time variations. In CFD studies pertaining to pollutant dispersion in street canyons, wind hourly average velocity is usually assumed as a reference value to simulate real world cases. Our results show that this input data must be completed with additional information about the extent of variation in wind intensity and its frequency in the hour.

Marine mammals are ecologically and culturally important species, and various countries have specific legislation to protect the welfare of individual marine mammals and the conservation of their populations. Anthropogenic noise represents a particular challenge for conservation and management. There is a large and growing body of research to support the conclusion that anthropogenic noise can affect marine mammal behavior, energetics, and physiology. The legal, policy, and management issues surrounding marine mammals and noise are similarly complex. Our objective is twofold. First, we discuss how policy and legal frameworks in Canada have some important differences from other jurisdictions covered in previous reviews, and provide a useful general case study. Secondly, we highlight some priority research areas that will improve marine mammal conservation and management. Our examples focus on the research needed to meet stated conservation objectives for marine mammal species in waters under Canadian jurisdiction.

A microfluidic chip was designed to assess the toxicity of pollutants in a high-throughput way by using marine phytoplankton motility as a sensor signal. In this chip, multiple gradient generators (CGGs) with diffusible chambers enable large scale of dose-response bioassays to be performed in a simple way. Two mobile marine phytoplankton cells were confined on-chip and stimulated by 8 concentrations (generated by CGG) of Hg, Pb, Cu and phenol singly, as well as Cu and phenol jointly. CASA system was used to characterize motility by motile percentage (%MOT), curvilinear velocity (VCL), average path velocity (VAP) and straight line velocity (VSL). In all cases, dose-dependent inhibitions of motility were observed. In the present system, only 2h was needed to predict EC50. Thus, the developed microfluidic chip device was proved to be useful as a rapid/simple and high-throughput test method in marine pollution toxicity assessment.

Preliminary impact assessment of two large oil spill incidents at Bodo Creek, in the Niger Delta, was undertaken, focusing on intertidal macrozoobenthos. Post-spill number of species and abundance were measured against recent pre-spill baseline data from the same study area. Results show that surface and infauna communities suffered severe reduction in abundance and number of species reduced by 81% after the spills, with two of the resampled sites having no taxa at all. Capitella capitata populations increased several-fold over pre-spill numbers. The impact of the oil spills is discussed in relation to local livelihoods and ecosystem integrity. The Niger Delta endemic lucinid, Keletistes rhizoecus, is proposed as a potential bioindicator for monitoring hydrocarbon contamination in Niger Delta estuaries.

A recent study demonstrated that 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) may have an adverse effect on the reproduction in marine medaka (Oryzias melastigma), but the molecular mechanisms remain largely unknown. In this study, we investigated the protein expression profiles of male and female gonads of O. melastigma exposed to dietary BDE-47 at two dosages (0.65 and 1.30μg/g/day, respectively) for 21days. Extracted proteins were labeled with iTRAQ and analyzed on a MALDI TOF/TOF analyzer, as results, 133 and 144 unique proteins were identified in testis and ovary, respective, and they exerted dose- and sex-dependent expression patterns. In testis, among the 42 differentially expressed proteins; down-regulation of histone variants and parvalbumins implicated BDE-47 may disrupt the spermatogenesis and induce sterility in fishes. In ovary, 38 proteins were differentially expressed; the elevation of vitellogenins and apolipoprotein A–I expression indicated BDE-47 acts as an estrogen-mimicking compound and led to reproductive impairment in O. melastigma.

We measured Cd and Pb in the muscle and stomach contents of Thunnus albacares and Katsuwonus pelamis to define the distribution of the elements in the tissues and their degrees of biomagnification. 210Po was measured in the livers of both species and compared to the results of similar studies. The trophic position of the tuna species was determined by N isotope measurements. The average activity of 210Po in the liver ranged from 119 to 157 (Bqkg−1 wet weight) in K. pelamis and T. albacares. The trophic position of T. albacares (4.60) was higher than that of K. pelamis (3.94). The Cd content of the muscle increased significantly with the trophic position of the tuna. δ13C in T. albacares and K. pelamis varied, with values of 3.13 and 1.88‰, respectively. The δ15N values in yellowfin tuna were higher than in skipjack tuna. The trophic position of T. albacares (4.60±0.67) was therefore more elevated than that of K. pelamis (3.94±1.06). Pb was biomagnified in T. albacares (transfer factor=1.46).