Tropospheric ozone in the surface air has become the primary atmospheric pollutant in Hangzhou, China, in recent years. Previous analysis is not enough to decode it for better regulation. Therefore, we use the traditional atmospheric model, Weather Research and Forecasting coupled with Community Multi-scale Air Quality (WRF-CMAQ), and machine learning models, Extreme Learning Machine (ELM), Multi-layer Perceptron (MLP), Random Forest (RF) and Recurrent Neural Network (RNN) to analyze and predict the ozone in the surface air in Hangzhou, China, using meteorology and air pollutants as input. We firstly quantitatively demonstrate that the dew-point deficit, instead of temperature and relative humidity, is the predominant meteorological factor in shaping tropospheric ozone. Urban heat island, daily direct solar radiation time, wind speed and wind direction play trivial role in impacting tropospheric ozone. NO₂ is the primary influential factors both for hourly ozone and daily O₃-8 h due to the titration effect. The most environmental-friendly way to mitigate the ozone pollution is to lower the volatile organic compounds (VOCs) with the highest ozone formation potentials. We deduce that the tropospheric ozone formation process tends to be not only non-linear but also non-smooth. Compared with the traditional atmospheric models, machine learning, whose characteristics are rapid convergence, short calculating time, adaptation of forecasting episodes, small program memory, higher accuracy and less cost, is able to predict tropospheric ozone more accurately.

Genotoxic analyses are commonly used in ecotoxicological studies as early biomarkers to investigate the potential effects of environmental contaminants on biological models. Several pollutants can induce DNA damage and, therefore, counting micronuclei and other nuclear abnormalities are efficient tools to evaluate genotoxicity. Some pollutants such as 4-nonylphenol (NP), a detergent used mainly in industries, and Cyproterone Acetate (CPA), an antiandrogenic medicine, have already shown genotoxic effects on some vertebrates. However, although amphibians are considered bioindicators of environmental quality and their populations are declining worldwide, the effects of these compounds on anurans are not yet known and, therefore, we believe that it is important to investigate such effects on anurans. Since water contamination is one of the ultimate causes of amphibian decline, ecotoxicological studies are important to discuss the appropriate solutions to avoid species extinction. Thus, this study investigates the genotoxic effects on Rana catesbeiana tadpoles and juveniles after being exposed to 1, 10 and 100 μg/L NP and 0.025, 0.25 and 2.5 ng/L CPA, by counting the nuclear abnormalities after exposure. The laboratory experiments lasted 28 days. The experimental conditions were the same except for the water volume since tadpoles and juveniles exhibit different habits at different developmental stages. Compared to juveniles, tadpoles were more susceptible to both compounds as indicated by the increased nuclear abnormalities observed in the highest NP concentration and all tested CPA concentrations. The juveniles, on the other hand, responded only to the two highest CPA concentrations. We concluded that CPA, even at very low concentrations, is extremely harmful to both anuran developmental stages and, particularly, to tadpoles. The significant effects observed on tadpoles is an important outcome of this study since 100 μg/L or higher NP concentrations are frequently detected in the environment.

The occurrence of microplastics (MPs) in the environment has been gaining widespread attention globally. MP-colonizing microorganisms are important links for MPs contamination in various ecosystems, but have not been well understood. To partially address this issue, the present study investigated biofilm formation by microorganisms originating from lake water on low-density polyethylene (LDPE) MPs using a cultivation approach and the surface-related effects on the MP-associated microbial communities using 16S rRNA high-throughput sequencing. With the addition of nonionic surfactants and UV-irradiation pretreatment that changed the surface properties of LDPE MPs, more microorganisms were colonized on LDPE surface. Microbial community analysis indicated that LDPE MPs were primarily colonized by the phyla Proteobacteria, Bacteroidetes and Firmicutes, and the surface roughness and hydrophobicity of MP were important factors shaping the LDPE MP-associated microbial community structure. Half of the top 20 most abundant genera colonizing on LDPE were found to be potential pathogens, e.g., plant pathogens Agrobacterium, nosocomial pathogens Chryseobacterium and fish pathogens Flavobacterium. This study demonstrated rapid bacterial colonization of LDPE MPs in lake water microcosms, the role of MPs as transfer vectors for harmful microorganisms in lake water, and provided a first glimpse into the effect of surface properties on LDPE MP-associated biofilm communities.

As a Chinese-specific alternative to perfluorooctane sulfonate (PFOS), 6:2 chlorinated polyfluorinated ether sulfonate (commercial name: F-53B) has been used in the metal plating industry for over 40 years. This prevalence of use has resulted in its subsequent detection within the environment, wildlife, and humans. Despite this, however, its hepatotoxic effects on aquatic organisms remain unclear. Here, we characterized the impacts of long-term F-53B exposure on adult zebrafish liver and their offspring. Results showed that the concentration of F-53B was greater in the F0 liver than that in the gonads and blood. Furthermore, males had significantly higher liver F-53B levels than females. Hepatomegaly and obvious cytoplasmic vacuolation indicated that F-53B exposure induced liver injury. Compared to control, liver triglyceride levels decreased by 30% and 33.5% in the 5 and 50 μg/L-exposed males and 22% in 50 μg/L-exposed females. Liver transcriptome analysis of F0 adult fish found 2175 and 1267 differentially expressed genes (DEGs) in the 5 μg/L-exposed males and females, respectively. Enrichment analyses further demonstrated that the effects of F-53B on hepatic transcripts were sex-dependent. Gene Ontology showed that most DEGs were involved in multicellular organism development in male fish, whereas in female fish, most DEGs were related to metabolic processes and gene expression. qRT-PCR analysis indicated that the PPAR signaling pathway likely contributed to F-53B-induced disruption of lipid metabolism in F0 adult fish. In F1 larvae (5 days post fertilization), the transcription of pparα increased, like that in F0 adult fish, but most target genes showed the opposite expression trends as their parents. Taken together, our research demonstrated chronic F-53B exposure adversely impacts zebrafish liver, with disruption of PPAR signaling pathway dependent on sex and developmental stage.

Although effluent from municipal wastewater treatment plants (WWTPs) is a major stressor in receiving environments, relatively few studies have addressed how its discharge affects natural fish communities. Here, we assessed fish community composition over three years along a gradient of effluent exposure from two distinct WWTPs within an International Joint Commission Area of Concern on the Great Lakes (Hamilton Harbour, Canada). We found that fish communities changed with distance from both WWTPs, and were highly dissimilar between sites that were closest to and furthest from the wastewater outfall. Despite differences in the size and treatment technology of the WWTPs and receiving habitats downstream, we found that the sites nearest the outfalls had the highest fish abundances and contained a common set of signature fish species (i.e., round goby Neogobius melanostomus, green sunfish Lepomis cyanellus). Non-native and stress tolerant species were also more abundant near one of the studied WWTPs when compared to the reference site, and the number of young-of-the-year fish collected did not vary along the effluent exposure gradients. Overall, we show that fish are attracted to wastewater outfalls raising the possibility that these sites may act as an ecological trap.

In this work PM₂.₅ was collected during winter and summer in a Sardinian village (Gonnostramatza, Italy) highly affected by biomass burning emissions. A multi-technique approach was adopted for the complete PM chemical characterization. The bulk characterization was performed by IC (Ion Chromatography), HPAEC (High-Performance Anion-Exchange Chromatography), TOT (Thermal Optical Transmittance) and ED-XRF (Energy-Dispersive X-Ray Fluorescence) while XPS (X-ray Photoelectron Spectroscopy) was used for the surface characterization. Using levoglucosan as specific tracer of biomass burning emissions, the assessment of the impact of this source was carried out and it represent the major PM source at the investigate site during winter. In winter the average levoglucosan concentration is 2096 ± 324 ng/m³ while during summer its concentration is negligible (18 ± 7 ng/m³). Levoglucosan content in PM₂.₅ during winter is on average 13.7%; it is estimated that 65% of PM₂.₅ is due to wood burning. XPS has been exploited in this work aiming at highlighting possible differences between surface and bulk composition of PM₂.₅. The surface of the particulate matter resulted enriched in carbon compared to the bulk. Among the components of XPS C1s signals recorded on the samples collected during winter, it was found that the signal at 286.5 eV, which is due to the presence of COH, reflects the bulk composition of levoglucosan.

Ubiquitous contamination of microplastics and arsenic in soil ecosystems can induce many health issues to nontarget soil organisms, and will also cause many potential threats to the gut bacterial communities of soil fauna. However, the changes in the gut bacterial communities of soil fauna after exposure to both microplastics and arsenic remain unknown. In this study, the toxicity and effects on the gut microbiota of earthworm Metaphire californica caused by the combined exposure of microplastics and arsenic were examined by using arsenic species analysis and high throughput sequencing of gut microbiota. Results showed that total arsenic and arsenic species in the earthworm gut and body tissues after exposure to combination of microplastics with arsenate (As(V)) were significantly different from that treated with As(V) alone. Microplastics lessened the accumulation of total arsenic and the transformation rate of As(V) to arsenite (As(III)). Microplastics alleviated the effect of arsenic on the gut microbiota possibly via adsorbing/binding As(V) and lowering arsenic bioavailability, thus prevented the reduction of As(V) and accumulation of total arsenic in the gut which resulted in a lower toxicity on the earthworm. The study broadens our understanding of the ecotoxicity of microplastics with other pollutants on the soil animals and on their gut microbiota.

Thallium (Tl) is a trace metal of severe toxicity. Its health concerns via consumption of contaminated vegetables have often been overlooked or underestimated. This study was designed to gain insight into the actual level and distribution characteristics of Tl and metal (loid)s (Pb, Cd, Cr, Sb, Mn, Cu, Zn, Ni, and Co) in agricultural soils and common vegetables cultivated in different zones (upstream, midstream, and downstream) of a densely populated residential area in a typical mine city, which has been open-pit exploiting Tl-bearing pyrite minerals since 1960s. The results show that most of the agricultural soils exhibit contaminated levels of Tl, with Tl contents (upstream: 1.35–4.31 mg/kg, midstream: 2.43–5.19 mg/kg, and downstream: 0.65–2.33 mg/kg) mostly exceeding the maximum permissible level (MPL) for agricultural land use (1 mg/kg). Sequential extraction procedure indicates that even Tl is predominantly retained in the residual fraction, significant levels of Tl are still present in the geochemically mobile fractions. Besides, metals like Cu, Cd, Mn, and Co are mostly distributed in the labile fractions. Almost all metal (loid)s in edible parts of the vegetables exceed their corresponding MPL for consumption. The chronic daily intake (CDI) and hazard quotient (HQ) values calculated for inhabitants at different ages indicate non-negligible Tl risks via consumption of local vegetables, especially for children. Therefore, it is critical to establish effective measures for hazardous waste management and enforceable regulations in Tl-polluted area to mitigate potential severe impacts of Tl on human health through food chain.

Human activities introduce significant contamination into aquatic systems that impact biodiversity and ecosystem function. Many contaminants accumulate, and remediation options are now required worldwide. One method for bioremediation involves the application of macrofauna to stimulate microbial ecosystem processes including contaminant removal. However, if we are to confidently apply such a technique, we need clarity on the effect of bioturbators on different contaminants and how these vary under different environmental scenarios. Here we used a systematic review and meta-analysis to analyse current knowledge on the activities of bioturbating macrofauna in contaminated sediments and quantify how bioturbation-bioremediation changes depend on the taxonomic group, the aquatic ecosystem and important environmental variables. Three common contaminant classes were reviewed and analysed: metals, nutrients (i.e. ammonia and phosphorous) and polycyclic aromatic hydrocarbons (PAH). In addition, meta-regressions were calculated to estimate the effect of environmental and experimental design variables on effect sizes. Meta-analytic results revealed that deeper burrowing and more active sediment surface animals (e.g. polychaetes) increased metal release from sediments, nutrients and oxygen uptake by microbial fractions in comparison to bioturbators that inhabit shallower depths in sediments. In addition, there was a different effect of bioturbators on response variables in different aquatic systems. Finally, bioturbator effects on nutrient and metal release appeared modulated by context-specific variables such as temperature, pH, sediment grain size, animal density and experimental duration. Our findings highlight critical knowledge gaps such as field applications, less studied macrobenthic fauna and the incorporation of molecular approaches. Our results provide the first quantitative synthesis of the effects of bioturbators on contaminant fate and the variables that need to be considered for the optimization of this method as a viable approach for sediment remediation and contaminant management in aquatic systems.

Phytostabilization of sulfidic PbZn tailing landscapes may be one of interim options of tailings management, but which is limited by acute phytotoxicity of heavy metals in the tailings. The present study aimed to investigate the effectiveness of soluble phosphate (i.e., K2HPO4) in immobilizing soluble Pb, Cd and Zn and lowering their acute phytotoxicity. The addition of soluble phosphate improved the growth of native plants Acacia chisholmii and survival rate of A. ligulata, where the latter exhibited 100% survival rate. This was in contrast to effects of conventional organic amendment in the tailings on metal solubility (e.g., elevated metal levels in porewater) and plant survival (e.g., only 42%). Organic amendment with mulch did not lower the levels of water-soluble Cd, Pb and Zn and their concentrations in plant tissues after 56 days of plant growth in the treatment. In contrast, the tailings amended with K2HPO4 significantly decreased metal concentrations in the porewater and plant tissues by about 80–92% and 56–88%, respectively. The metal immobilization by phosphate was due to the formation of insoluble or sparingly soluble metal (Pb, Cd and Zn)-phosphate minerals in the tailings with circumneutral pH conditions, as revealed by using X-ray diffraction and scanning electron microanalyses. The reduced metal concentrations in roots and shoots of Acacia species after direct root contact with the K2HPO4 amended tailings suggested that metals (i.e., Pb, Cd and Zn) were effectively immobilized by the phosphate treatment of the tailings. These findings indicate that addition of high dosage of soluble phosphate may provide a low cost option to treat sulfidic PbZn tailings for rapid phytostabilization of the tailings surface, as an interim option to manage environmental risks of sulfidic PbZn tailings.