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Characteristics and source appointment of atmospheric particulate mercury over East China Sea: Implication on the deposition of atmospheric particulate mercury in marine environment Texte intégral
2017
Duan, Lian | Cheng, Na | Xiu, Guangli | Wang, Fujiang | Chen, Ying
Total Suspended Particulate (TSP) samples were collected at Huaniao Island in northern East China Sea (ECS) from March 2012 to January 2013. Chemical analysis were conducted to measure the concentration of total particulate mercury (TPM) and speciated particulate mercury including HCl-soluble particulate mercury (HPM), elemental particulate mercury (EPM) and residual particulate mercury (RPM). The bromine (Br) and iodine (I) on particles were also detected. The mean concentration of TPM during the study period was 0.23 ± 0.15 ng m⁻³, while the obviously seasonal variation was found that the concentrations of TPM in spring, summer, fall and winter were 0.34 ± 0.20 ng m⁻³, 0.15 ± 0.03 ng m⁻³, 0.15 ± 0.05 ng m⁻³ and 0.27 ± 0.26 ng m⁻³, respectively. The statistically strong correlation of bromine and iodine to HPM was only found in spring with r = 0.81 and 0.77 (p < 0.01), respectively. While the strongest correlations between EPM and bromine and iodine were found in winter with r = 0.92 (Br) and 0.96 (I) (p < 0.01), respectively. The clustered 72-h backward trajectories of different seasons and the whole sampling period were categorized into 4 groups. In spring, the clusters passed a long distance across the East China Sea and brought about low concentration of mercury due to the deposition of mercury over the sea. The cluster of air mass across the sea had low concentration of HPM in winter, which suggested that the oxidation of mercury in winter might be related to other oxidants. During the whole sampling period, the air mass from the north of China contributed to the higher concentration of TPM in Huaniao Island.
Afficher plus [+] Moins [-]Total tin and organotin speciation in historic layers of antifouling paint on leisure boat hulls Texte intégral
2017
Lagerström, Maria | Strand, Jakob | Eklund, Britta | Ytreberg, Erik
Despite their ban on small vessels in 1989 in the EU, organotin compounds (OTCs) are still being released into the environment due to their presence in historic paint layers on leisure boats. 23 paint samples scraped from recreational boats from three countries around the Baltic Sea were analyzed for total tin (Sn) and OTCs. Two antifouling paint products were also subjected to the same analyses. A new method for the detection of Sn in paint flake samples was developed and found to yield more accurate results compared to four different acid digestion methods. A new method was also developed for the extraction of OTCs from ground paint flakes. This endeavor revealed that existing methods for organotin analysis of sediment may not have full recoveries of OTCs if paint flakes are present in the sample. The hull paint samples had Sn concentrations ranging from 25 to 18,000 mg/kg paint and results showed that tributyltin (TBT) was detected in all samples with concentrations as high as 4.7 g (as Sn)/kg paint. TBT was however not always the major OTC. Triphenyltin (TPhT) was abundant in many samples, especially in those originating from Finland. Several other compounds such as monobutyltin (MBT), dibutyltin (DBT), tetrabutyltin (TeBT), monophenyltin (MPhT) and diphenyltin (DPhT) were also detected. These could be the result of degradation occurring on the hull or of impurities in the paint products as they were also identified in the two analyzed paint products. A linear correlation (r2 = 0.934) was found between the total tin content and the sum of all detected OTCs. The detection of tin can therefore be used to indicate the presence of OTCs on leisure boats.
Afficher plus [+] Moins [-]Phenanthrene-triggered Chlorosis is caused by elevated Chlorophyll degradation and leaf moisture Texte intégral
2017
Shen, Yu | Li, Jinfeng | Gu, Ruochen | Yue, Le | Zhan, Xinhua | Xing, Baoshan
Leaf is an important organ in responding to environmental stresses. To date, chlorophyll metabolism under polycyclic aromatic hydrocarbon (PAH) stress is still unclear. Here we reveal, for the first time, the chlorophyll metabolism of wheat seedling leaves in response to phenanthrene (a model PAH) exposure. In this study, the hydroponic experiment was employed, and the wheat seedlings were exposed to phenanthrene to observe the response at day 1, 3, 5, 7 and 9. Over the exposure time, wheat leaf color turns light. With the accumulation of phenanthrene, the concentrations of glutamate, 5-aminolevulinic acid, uroporphyrinogen III, protoporphyrin IX, Mg-protoporphyrin IX and protochlorophyllide increase while the concentrations of porphobilinogen and Chlorophyll b decrease. Also chlorophyll a content rises initially and then declines. Uroporphyrinogen III synthase and chlorophyllase are activated and porphobilinogen deaminase activity declines in the treatments. Both chlorophyll synthesis and degradation are enhanced, but the degradation rate is faster. Phenanthrene accumulation has significant and positive effects on increase of glutamate, 5-aminolevulinic acid, uroporphyrinogen III, protoporphyrin IX, Mg-protoporphyrin IX and protochlorophyllide concentrations. There is a negative correlation between phenanthrene accumulation and total chlorophyll. Additionally, the leaf moisture increases. Therefore, it is concluded that wheat leaf chlorosis results from a combination of accelerated chlorophyll degradation and elevated leaf moisture under phenanthrene exposure. Our results are helpful not only for better understanding the toxicity of PAHs to plants and crop PAH-adaptive mechanism in the environment, but also for potentially employing the changes of the chlorophyll-synthesizing precursors and enzyme activities in plant leaves as indicators of plant response to PAH pollution.
Afficher plus [+] Moins [-]Environmental hazard assessment of a marine mine tailings deposit site and potential implications for deep-sea mining Texte intégral
2017
Mestre, Nélia C. | Rocha, Thiago L. | Canals, M. (Miquel) | Cardoso, Cátia | Danovaro, Roberto | Dell’Anno, Antonio | Gambi, Cristina | Regoli, Francesco | Sanchez-Vidal, Anna | Bebianno, Maria João
Environmental hazard assessment of a marine mine tailings deposit site and potential implications for deep-sea mining Texte intégral
2017
Mestre, Nélia C. | Rocha, Thiago L. | Canals, M. (Miquel) | Cardoso, Cátia | Danovaro, Roberto | Dell’Anno, Antonio | Gambi, Cristina | Regoli, Francesco | Sanchez-Vidal, Anna | Bebianno, Maria João
Portmán Bay is a heavily contaminated area resulting from decades of metal mine tailings disposal, and is considered a suitable shallow-water analogue to investigate the potential ecotoxicological impact of deep-sea mining. Resuspension plumes were artificially created by removing the top layer of the mine tailings deposit by bottom trawling. Mussels were deployed at three sites: i) off the mine tailings deposit area; ii) on the mine tailings deposit beyond the influence from the resuspension plumes; iii) under the influence of the artificially generated resuspension plumes. Surface sediment samples were collected at the same sites for metal analysis and ecotoxicity assessment. Metal concentrations and a battery of biomarkers (oxidative stress, metal exposure, biotransformation and oxidative damage) were measured in different mussel tissues. The environmental hazard posed by the resuspension plumes was investigated by a quantitative weight of evidence (WOE) model that integrated all the data. The resuspension of sediments loaded with metal mine tails demonstrated that chemical contaminants were released by trawling subsequently inducing ecotoxicological impact in mussels’ health. Considering as sediment quality guidelines (SQGs) those indicated in Spanish action level B for the disposal of dredged material at sea, the WOE model indicates that the hazard is slight off the mine tailings deposit, moderate on the mine tailings deposit without the influence from the resuspension plumes, and major under the influence of the resuspension plumes. Portmán Bay mine tailings deposit is a by-product of sulphide mining, and despite differences in environmental setting, it can reflect the potential ecotoxic effects to marine fauna from the impact of resuspension of plumes created by deep-sea mining of polymetallic sulphides. A similar approach as in this study could be applied in other areas affected by sediment resuspension and for testing future deep-sea mining sites in order to assess the associated environmental hazards.
Afficher plus [+] Moins [-]Environmental hazard assessment of a marine mine tailings deposit site and potential implications for deep-sea mining Texte intégral
2017
Mestre, Nélia | Rocha, Thiago L. | Canals, Miquel | Cardoso, Cátia | Danovaro, Roberto | Dell’Anno, Antonio | Gambi, Cristina | Regoli, Francesco | Sanchez-Vidal, Anna | Bebianno, Maria João
Portmán Bay is a heavily contaminated area resulting from decades of metal mine tailings disposal, and is considered a suitable shallow-water analogue to investigate the potential ecotoxicological impact of deep-sea mining. Resuspension plumes were artificially created by removing the top layer of the mine tailings deposit by bottom trawling. Mussels were deployed at three sites: i) off the mine tailings deposit area; ii) on the mine tailings deposit beyond the influence from the resuspension plumes; iii) under the influence of the artificially generated resuspension plumes. Surface sediment samples were collected at the same sites for metal analysis and ecotoxicity assessment. Metal concentrations and a battery of biomarkers (oxidative stress, metal exposure, biotransformation and oxidative damage) were measured in different mussel tissues. The environmental hazard posed by the resuspension plumes was investigated by a quantitative weight of evidence (WOE) model that integrated all the data. The resuspension of sediments loaded with metal mine tails demonstrated that chemical contaminants were released by trawling subsequently inducing ecotoxicological impact in mussels' health. Considering as sediment quality guidelines (SQGs) those indicated in Spanish action level B for the disposal of dredged material at sea, the WOE model indicates that the hazard is slight off the mine tailings deposit, moderate on the mine tailings deposit without the influence from the resuspension plumes, and major under the influence of the resuspension plumes. Portmán Bay mine tailings deposit is a by-product of sulphide mining, and despite differences in environmental setting, it can reflect the potential ecotoxic effects to marine fauna from the impact of resuspension of plumes created by deep-sea mining of polymetallic sulphides. A similar approach as in this study could be applied in other areas affected by sediment resuspension and for testing future deep-sea mining sites in order to assess the associated environmental hazards. | info:eu-repo/semantics/publishedVersion
Afficher plus [+] Moins [-]Impact of commuting exposure to traffic-related air pollution on cognitive development in children walking to school Texte intégral
2017
Impact of commuting exposure to traffic-related air pollution on cognitive development in children walking to school Texte intégral
2017
A few studies have found associations between the exposure to traffic-related air pollution at school and/or home and cognitive development. The impact on cognitive development of the exposure to air pollutants during commuting has not been explored. We aimed to assess the role of the exposure to traffic-related air pollutants during walking commute to school on cognitive development of children. We performed a longitudinal study of children (n = 1,234, aged 7–10 y) from 39 schools in Barcelona (Catalonia, Spain) who commuted by foot to school. Children were tested four times during a 12-month follow-up to characterize their developmental trajectories of working memory (d’ of the three-back numbers test) and inattentiveness (hit reaction time standard error of the Attention Network Test). Average particulate matter ≤2.5 μm (PM2.5), Black Carbon (BC) and NO2 concentrations were estimated using Land Use Regression for the shortest walking route to school. Differences in cognitive growth were evaluated by linear mixed effects models with age-by-pollutant interaction terms. Exposure to PM2.5 and BC from the commutes by foot was associated with a reduction in the growth of working memory (an interquartile range increase in PM2.5 and BC concentrations decreased the annual growth of working memory by 5.4 (95% CI [-10.2, -0.6]) and 4.6 (95% CI [-9.0, -0.1]) points, respectively). The findings for NO2 were not conclusive and none of the pollutants were associated with inattentiveness. Efforts should be made to implement pedestrian school pathways through low traffic streets in order to increase security and minimize children's exposure to air pollutants.
Afficher plus [+] Moins [-]Impact of commuting exposure to traffic-related air pollution on cognitive development in children walking to school Texte intégral
2017
Alvarez-Pedrerol, Mar | Rivas, Ioar | López-Vicente, Mònica | Suades-González, Elisabet | Donaire-Gonzalez, David | Cirach, Marta | de Castro, Montserrat | Esnaola, Mikel | Basagaña, Xavier | Dadvand, Payam | Nieuwenhuijsen, Mark | Sunyer, Jordi | 0000-0002-4743-619X | 0000-0003-2337-1712
A few studies have found associations between the exposure to traffic-related air pollution at school and/or home and cognitive development. The impact on cognitive development of the exposure to air pollutants during commuting has not been explored. We aimed to assess the role of the exposure to traffic-related air pollutants during walking commute to school on cognitive development of children. We performed a longitudinal study of children (n = 1,234, aged 7-10 y) from 39 schools in Barcelona (Catalonia, Spain) who commuted by foot to school. Children were tested four times during a 12-month follow-up to characterize their developmental trajectories of working memory (d' of the three-back numbers test) and inattentiveness (hit reaction time standard error of the Attention Network Test). Average particulate matter ≤2.5 μm (PM2.5), Black Carbon (BC) and NO2 concentrations were estimated using Land Use Regression for the shortest walking route to school. Differences in cognitive growth were evaluated by linear mixed effects models with age-by-pollutant interaction terms. Exposure to PM2.5 and BC from the commutes by foot was associated with a reduction in the growth of working memory (an interquartile range increase in PM2.5 and BC concentrations decreased the annual growth of working memory by 5.4 (95% CI [-10.2, -0.6]) and 4.6 (95% CI [-9.0, -0.1]) points, respectively). The findings for NO2 were not conclusive and none of the pollutants were associated with inattentiveness. Efforts should be made to implement pedestrian school pathways through low traffic streets in order to increase security and minimize children's exposure to air pollutants. | The research leading to these results has received funding from the European Community's Seventh Framework Program (ERC-Advanced Grant) under grant agreement number 268479 – the BREATHE project. Special thanks go to all the participant families and schools. We also acknowledge Cecilia Persavento, Judit González, Laura Bouso, and Pere Figueras for their contribution to the fieldwork. | Peer reviewed
Afficher plus [+] Moins [-]Metabolic disruption of zebrafish (Danio rerio) embryos by bisphenol A. An integrated metabolomic and transcriptomic approach Texte intégral
2017
Ortiz-Villanueva, Elena | Navarro-Martín, Laia | Jaumot, Joaquim | Benavente, Fernando | Sanz-Nebot, Victoria | Piña, Benjamín | Tauler, Romà
Metabolic disruption of zebrafish (Danio rerio) embryos by bisphenol A. An integrated metabolomic and transcriptomic approach Texte intégral
2017
Ortiz-Villanueva, Elena | Navarro-Martín, Laia | Jaumot, Joaquim | Benavente, Fernando | Sanz-Nebot, Victoria | Piña, Benjamín | Tauler, Romà
Although bisphenol A (BPA) is commonly recognized as an endocrine disruptor, the metabolic consequences of its exposure are still poorly understood. In this study, we present a non-targeted LC-MS based metabolomic analysis in combination with a full-genome, high-throughput RNA sequencing (RNA-Seq) to reveal the metabolic effects and the subjacent regulatory pathways of exposing zebrafish embryos to BPA during the first 120 hours post-fertilization. We applied multivariate data analysis methods to extract biochemical information from the LC-MS and RNA-Seq complex datasets and to perform testable predictions of the phenotypic adverse effects. Metabolomic and transcriptomic data revealed a similar subset of altered pathways, despite the large difference in the number of identified biomarkers (around 50 metabolites and more than 1000 genes). These results suggest that even a moderate coverage of zebrafish metabolome may be representative of the global metabolic changes. These multi-omic responses indicate a specific metabolic disruption by BPA affecting different signaling pathways, such as retinoid and prostaglandin metabolism. The combination of transcriptomic and metabolomic data allowed a dynamic interpretation of the results that could not be drawn from either single dataset. These results illustrate the utility of -omic integrative analyses for characterizing the physiological effects of toxicants beyond the mere indication of the affected pathways.
Afficher plus [+] Moins [-]Metabolic disruption of zebrafish (Danio rerio) embryos by bisphenol A. An integrated metabolomic and transcriptomic approach Texte intégral
2017
Ortiz-Villanueva, Elena | Navarro-Martín, Laia | Jaumot, Joaquim | Benavente, Fernando | Sanz-Nebot, Victoria M. | Piña, Benjamín | Tauler, Romà | European Commission
Although bisphenol A (BPA) is commonly recognized as an endocrine disruptor, the metabolic consequences of its exposure are still poorly understood. In this study, we present a non-targeted LC-MS based metabolomic analysis in combination with a full-genome, high-throughput RNA sequencing (RNA-Seq) to reveal the metabolic effects and the subjacent regulatory pathways of exposing zebrafish embryos to BPA during the first 120 hours post-fertilization. We applied multivariate data analysis methods to extract biochemical information from the LC-MS and RNA-Seq complex datasets and to perform testable predictions of the phenotypic adverse effects. Metabolomic and transcriptomic data revealed a similar subset of altered pathways, despite the large difference in the number of identified biomarkers (around 50 metabolites and more than 1000 genes). These results suggest that even a moderate coverage of zebrafish metabolome may be representative of the global metabolic changes. These multi-omic responses indicate a specific metabolic disruption by BPA affecting different signaling pathways, such as retinoid and prostaglandin metabolism. The combination of transcriptomic and metabolomic data allowed a dynamic interpretation of the results that could not be drawn from either single dataset. These results illustrate the utility of -omic integrative analyses for characterizing the physiological effects of toxicants beyond the mere indication of the affected pathways. © 2017 Elsevier Ltd | This work was supported by the European Research Council under the European Union's Seventh Framework Programme (FP/ 2007-2013)/ERC Grant Agreement n. 320737. Some part of this study was also supported by a grant from the Spanish Ministry of Economy and Competitiveness (CTQ2014-56777-R). LNM was supported by a Beatriu de Pinos Postdoctoral Fellow (2013BP-B- 00088) awarded by the Secretary for Universities and Research of the Ministry of Economy and Knowledge of the Government of Catalonia and the Cofund programme of the Marie Curie Actions of the 7th R&D Framework Programme of the European Union. | Peer reviewed
Afficher plus [+] Moins [-]CO variability and its association with household cooking fuels consumption over the Indo-Gangetic Plains Texte intégral
2017
Pandey, Alok Kumar | Mishra, Amit Kumar | Kumar, Ritesh | Berwal, Shivesh | Devadas, Rakhesh | Huete, Alfredo | Kumar, Krishan
CO variability and its association with household cooking fuels consumption over the Indo-Gangetic Plains Texte intégral
2017
Pandey, Alok Kumar | Mishra, Amit Kumar | Kumar, Ritesh | Berwal, Shivesh | Devadas, Rakhesh | Huete, Alfredo | Kumar, Krishan
This study examines the spatio-temporal trends obtained from decade long (Jan 2003–Dec 2014) satellite observational data of Atmospheric Infrared Sounder (AIRS) and Measurements of Pollution in the Troposphere (MOPITT) on carbon monoxide (CO) concentration over the Indo-Gangetic Plains (IGP) region. The time sequence plots of columnar CO levels over the western, central and eastern IGP regions reveal marked seasonal behaviour, with lowest CO levels occurring during the monsoon months and the highest CO levels occurring during the pre-monsoon period. A negative correlation between CO levels and rainfall is observed. CO vertical profiles show relatively high values in the upper troposphere at ∼200 hPa level during the monsoon months, thus suggesting the role of convective transport and advection in addition to washout behind the decreased CO levels during this period. MOPITT and AIRS observations show a decreasing trend of 9.6 × 1015 and 1.5 × 1016 molecules cm−2 yr−1, respectively, in columnar CO levels over the IGP region. The results show the existence of a spatial gradient in CO from the eastern (higher levels) to western IGP region (lower levels). Data from the Census of India on the number of households using various cooking fuels in the IGP region shows the prevalence of biomass-fuel (i.e. firewood, crop residue, cowdung etc.) use over the eastern and central IGP regions and that of liquefied petroleum gas over the western IGP region. CO emission estimates from cooking activity over the three IGP regions are found to be in the order east > central > west, which support the existence of the spatial gradient in CO from eastern to the western IGP region. Our results support the intervention of present Indian government on limiting the use of biomass-fuels in domestic cooking to achieve the benefits in terms of the better air quality, household health and regional/global climate change mitigation.
Afficher plus [+] Moins [-]CO variability and its association with household cooking fuels consumption over the Indo-Gangetic Plains Texte intégral
2016
Pandey, AK | Mishra, AK | Kumar, R | Berwal, S | Devadas, R | Huete, A | Kumar, K
© 2017 Elsevier Ltd This study examines the spatio-temporal trends obtained from decade long (Jan 2003–Dec 2014) satellite observational data of Atmospheric Infrared Sounder (AIRS) and Measurements of Pollution in the Troposphere (MOPITT) on carbon monoxide (CO) concentration over the Indo-Gangetic Plains (IGP) region. The time sequence plots of columnar CO levels over the western, central and eastern IGP regions reveal marked seasonal behaviour, with lowest CO levels occurring during the monsoon months and the highest CO levels occurring during the pre-monsoon period. A negative correlation between CO levels and rainfall is observed. CO vertical profiles show relatively high values in the upper troposphere at ∼200 hPa level during the monsoon months, thus suggesting the role of convective transport and advection in addition to washout behind the decreased CO levels during this period. MOPITT and AIRS observations show a decreasing trend of 9.6 × 1015and 1.5 × 1016molecules cm−2yr−1, respectively, in columnar CO levels over the IGP region. The results show the existence of a spatial gradient in CO from the eastern (higher levels) to western IGP region (lower levels). Data from the Census of India on the number of households using various cooking fuels in the IGP region shows the prevalence of biomass-fuel (i.e. firewood, crop residue, cowdung etc.) use over the eastern and central IGP regions and that of liquefied petroleum gas over the western IGP region. CO emission estimates from cooking activity over the three IGP regions are found to be in the order east > central > west, which support the existence of the spatial gradient in CO from eastern to the western IGP region. Our results support the intervention of present Indian government on limiting the use of biomass-fuels in domestic cooking to achieve the benefits in terms of the better air quality, household health and regional/global climate change mitigation.
Afficher plus [+] Moins [-]Ecotoxicoproteomic assessment of the functional alterations caused by chronic metallic exposures in gammarids Texte intégral
2017
Gismondi, E. | Thomé, J.-P. | Urien, N. | Uher, E. | Baiwir, D. | Mazzucchelli, G. | De Pauw, E. | Fechner, L.C. | Lebrun, J.D.
Ecotoxicoproteomic assessment of the functional alterations caused by chronic metallic exposures in gammarids Texte intégral
2017
Gismondi, E. | Thomé, J.-P. | Urien, N. | Uher, E. | Baiwir, D. | Mazzucchelli, G. | De Pauw, E. | Fechner, L.C. | Lebrun, J.D.
Very few ecotoxicological studies have been performed on long-term exposure under controlled conditions, hence limiting the assessment of the impact of chronic and diffuse chemical pressures on the health of aquatic organisms. In this study, an ecotoxicoproteomic approach was used to assess the integrated response and possible acclimation mechanisms in Gammarus fossarum following chronic exposures to Cd, Cu or Pb, at environmentally realistic concentrations (i.e. 0.25, 1.5 and 5 μg/L respectively). After 10-week exposure, changes in protein expression were investigated in caeca of control and exposed males. Gel-free proteomic analyses allowed for the identification of 35 proteins involved in various biological functions, for which 23 were significantly deregulated by metal exposures. The protein deregulation profiles were specific to each metal, providing evidence for metal-specific action sites and responses of gammarids. Among the tested metals, Cu was the most toxic in terms of mortality, probably linked with persistent oxidative stress. Moulting and osmoregulation were the major biological functions affected by Cu in the long-term. In Pb-exposed gammarids, significant deregulations of proteins involved in immune response and cytoskeleton were observed. Reproduction appears to be strongly affected in gammarids chronically exposed to Cd or Pb. Besides, modified expressions of several proteins involved in energy transfer and metabolism highlighted important energetic reshuffling to cope with chronic metal exposures. These results support the fact that metallic pressures induce a functional and energetic cost for individuals of G. fossarum with potential repercussions on population dynamics. Furthermore, this ecotoxicoproteomic study offers promising lines of enquiry in the development of new biomarkers that could make evidence of long-term impacts of metals on the health of organisms.
Afficher plus [+] Moins [-]Ecotoxicoproteomic assessment of the functional alterations caused by chronic metallic exposures in gammarids Texte intégral
2017
Gismondi, E. | Thomé, J.-P. | Urien, N. | Uher, E. | Baiwir, D. | Mazzucchelli, G. | de Pauw, E. | Fechner, L.C. | Lebrun, Jérémie, D. | Freshwater and Oceanic Sciences Unit of reSearch (FOCUS) ; Laboratory of Oceanology ; Université de Liège-Université de Liège | Laboratory of Animal Ecology and Ecotoxicity (LEAE-CART) ; Université de Liège | Université de Liège | Hydrosystèmes et Bioprocédés (UR HBAN) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Mass Spectrometry Laboratory (MS LAB) ; Université de Liège-Centre d'Analyse des Résidus en Traces-Groupe Interdisciplinaire de Génoprotéomique Appliquée
Ecotoxicoproteomic assessment of the functional alterations caused by chronic metallic exposures in gammarids Texte intégral
2017
Gismondi, Eric | Thomé, Jean-Pierre | Urien, Nastassia | Uher, Emmanuelle | Baiwir, Dominique | Mazzucchelli, Gabriel | De Pauw, Edwin | Fechner, Lise | Lebrun, Jérémie | FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège
peer reviewed | Very few ecotoxicological studies have been performed on long-term exposure under controlled conditions, hence limiting the assessment of the impact of chronic and diffuse chemical pressures on the health of aquatic organisms. In this study, an ecotoxicoproteomic approach was used to assess the integrated response and possible acclimation mechanisms in Gammarus fossarum following chronic exposures to Cd, Cu or Pb, at environmentally realistic concentrations (i.e. 0.25, 1.5 and 5 µg/L respectively). After 10-week exposure, changes in protein expression were investigated in caeca of control and exposed males. Gel-free proteomic analyses allowed for the identification of 35 proteins involved in various biological functions, for which 23 were significantly deregulated by metal exposures. The protein deregulation profiles were specific to each metal, providing evidence for metal-specific action sites and responses of gammarids. Among the tested metals, Cu was the most toxic in terms of mortality, probably linked with persistent oxidative stress. Moulting and osmoregulation were the major biological functions affected by Cu in the long-term. In Pb-exposed gammarids, significant deregulations of proteins involved in immune response and cytoskeleton were observed. Reproduction appears to be strongly affected in gammarids chronically exposed to Cd or Pb. Besides, modified expressions of several proteins involved in energy transfer and metabolism highlighted important energetic reshuffling to cope with chronic metal exposures. These results support the fact that metallic pressures induce a functional and energetic cost for individuals of G. fossarum with potential repercussions on population dynamics. Furthermore, this ecotoxicoproteomic study offers promising lines of enquiry in the development of new biomarkers that could make evidence of long-term impacts of metals on the health of organisms.
Afficher plus [+] Moins [-]Ecotoxicoproteomic assessment of the functional alterations caused by chronic metallic exposures in gammarids Texte intégral
2017
Gismondi, Éric | Thomé, Jean-Pierre | Urien, Nastassia | Uher, Emmanuelle | Baiwir, Dominique | Mazzucchelli, Gabriel | de Pauw, Edwin | Fechner, Lise, C. | Lebrun, Jérémie, D. | Laboratoire des Interactions Ecotoxicologie, Biodiversité, Ecosystèmes (LIEBE) ; Université Paul Verlaine - Metz (UPVM)-Centre National de la Recherche Scientifique (CNRS) | Hydrosystèmes et Bioprocédés (UR HBAN) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Institut National de la Recherche Scientifique [Québec] (INRS) | Fédération Île-de-France de Recherche sur l'Environnement (FIRE) ; Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-AgroParisTech-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Mass Spectrometry Laboratory (MS LAB) ; Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège)-Centre d'Analyse des Résidus en Traces-Groupe Interdisciplinaire de Génoprotéomique Appliquée | Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) | ANR-12-ADAP-0003,SequAdapt,Conséquences et mécanismes de l'adaptation à la contamination chimique en Seine(2012)
International audience
Afficher plus [+] Moins [-]Relationships between metal compartmentalization and biomarkers in earthworms exposed to field-contaminated soils Texte intégral
2017
Beaumelle, Léa | Hedde, Mickaël | Vandenbulcke, Franck | Lamy, Isabelle
Relationships between metal compartmentalization and biomarkers in earthworms exposed to field-contaminated soils Texte intégral
2017
Beaumelle, Léa | Hedde, Mickaël | Vandenbulcke, Franck | Lamy, Isabelle
Partitioning tissue metal concentration into subcellular compartments reflecting toxicologically available pools may provide good descriptors of the toxicological effects of metals on organisms. Here we investigated the relationships between internal compartmentalization of Cd, Pb and Zn and biomarker responses in a model soil organism: the earthworm. The aim of this study was to identify metal fractions reflecting the toxic pressure in an endogeic, naturally occurring earthworm species (Aporrectodea caliginosa) exposed to realistic field-contaminated soils.After a 21 days exposure experiment to 31 field-contaminated soils, Cd, Pb and Zn concentrations in earthworms and in three subcellular fractions (cytosol, debris and granules) were quantified. Different biomarkers were measured: the expression of a metallothionein gene (mt), the activity of catalase (CAT) and of glutathione-s-transferase (GST), and the protein, lipid and glycogen reserves. Biomarkers were further combined into an integrated biomarker index (IBR).The subcellular fractionation provided better predictors of biomarkers than the total internal contents hence supporting its use when assessing toxicological bioavailability of metals to earthworms. The most soluble internal pools of metals were not always the best predictors of biomarker responses. metallothionein expression responded to increasing concentrations of Cd in the insoluble fraction (debris + granules). Protein and glycogen contents were also mainly related to Cd and Pb in the insoluble fraction. On the other hand, GST activity was better explained by Pb in the cytosolic fraction. CAT activity and lipid contents variations were not related to metal subcellular distribution. The IBR was best explained by both soluble and insoluble fractions of Pb and Cd.This study further extends the scope of mt expression as a robust and specific biomarker in an ecologically representative earthworm species exposed to field-contaminated soils. The genetic lineage of the individuals, assessed by DNA barcoding with cytochrome c oxidase subunit I, did not influence mt expression.
Afficher plus [+] Moins [-]Relationships between metal compartmentalization and biomarkers in earthworms exposed to field-contaminated soils Texte intégral
2017
Beaumelle, Léa | Hedde, Mickael | Vandenbulcke, Franck | Lamy, Isabelle | Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515 (LGCgE) ; Université d'Artois (UA)-Université de Lille-Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai) ; Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-JUNIA (JUNIA) ; Université catholique de Lille (UCL)-Université catholique de Lille (UCL) | ANR-11-INBS-0001,ANAEE-FR,ANAEE-Services(2011)
Partitioning tissue metal concentration into subcellular compartments relecting 'toxicologically available'pools may provide good descriptors of the toxicological efects of metals on organisms. Here we investigatedthe relationships between internal compartmentalization of Cd, Pb and Zn and biomarker responses in a model soil organism: the earthworm. The aim of this study was to identify metal fractions reflecting the toxic pressure in an endogeic, naturally occurring earthworm species (Aporrectodea caliginosa) exposed torealistic field-contaminated soils.After a 21 days exposure experiment to 31 field contaminated soils, Cd, Pb and Zn concentrationsin earthworms and in three subcellular fractions (cytosol, debris and granules) were quantified. Differentbiomarkers were measured: the expression of a metallothionein gene (mt), the activity of catalase (CAT) andof glutathione-s-transferase (GST), and the protein, lipid and glycogen reserves. Biomarkers were furthercombined in an integrated biomarker index (IBR).The subcellular fractionation provided better predictors of biomarkers than the total internal contentshence supporting its use when assessing toxicological bioavailability of metals to earthworms. The mostsoluble internal pools of metals were not always the best predictors of biomarker responses. metallothioneinexpression responded to increasing concentrations of Cd in the insoluble fraction (debris + granules). Proteinand glycogen contents were also mainly related to Cd and Pb in the insoluble fraction. On the other hand,GST activity was better explained by Pb in the cytosolic fraction. CAT activity and lipid contents variationswere not related to metal subcellular distribution. The IBR was best explained by both soluble and insolublefractions of Cd and Pb.This study further extends the scope of mt expression as a robust and specific biomarker in an ecologicallyrepresentative earthworm species exposed to field-contaminated soils. The genetic lineage of the individuals,assessed by DNA barcoding with cytochrome c oxidase subunit I, did not inuence mt expression.
Afficher plus [+] Moins [-]Role of oxygen-containing functional groups in forest fire-generated and pyrolytic chars for immobilization of copper and nickel Texte intégral
2017
Esfandbod, Maryam | Merritt, Christopher R. | Rashti, Mehran Rezaei | Singh, Balwant | Boyd, Sue E. | Srivastava, Prashant | Brown, Christopher L. | Butler, Orpheus M. | Kookana, Rai S. | Chen, Chengrong
Role of oxygen-containing functional groups in forest fire-generated and pyrolytic chars for immobilization of copper and nickel Texte intégral
2017
Esfandbod, Maryam | Merritt, Christopher R. | Rashti, Mehran Rezaei | Singh, Balwant | Boyd, Sue E. | Srivastava, Prashant | Brown, Christopher L. | Butler, Orpheus M. | Kookana, Rai S. | Chen, Chengrong
Char as a carbon-rich material, can be produced under pyrolytic conditions, wildfires or prescribed burn offs for fire management. The objective of this study was to elucidate mechanistic interactions of copper (Cu2+) and nickel (Ni2+) with different chars produced by pyrolysis (green waste, GW; blue-Mallee, BM) and forest fires (fresh-burnt by prescribed fire, FC; aged char produced by wild fire, AC). The pyrolytic chars were more effective sorbents of Cu2+ (∼11 times) and Ni2+ (∼5 times) compared with the forest fire chars. Both cross-polarization (CPMAS-NMR) and Bloch decay (BDMAS-NMR) 13C NMR spectroscopies showed that forest fire chars have higher woody components (aromatic functional groups) and lower polar groups (e.g. O-alkyl C) compared with the pyrolytic chars. The polarity index was greater in the pyrolytic chars (0.99–1.34) than in the fire-generated chars (0.98–1.15), while aromaticity was lower in the former than in the latter. Fourier transform infrared (FTIR) and Raman spectroscopies indicated the binding of carbonate and phosphate with both Cu2+ and Ni2+ in all chars, but with a greater extent in pyrolytic than forest fire-generated chars. These findings have demonstrated the key role of char's oxygen-containing functional groups in determining their sorption capacity for the Cu2+ and Ni2+ in contaminated lands.
Afficher plus [+] Moins [-]Role of oxygen-containing functional groups in forest fire-generated and pyrolytic chars for immobilization of copper and nickel Texte intégral
2017
Esfandbod, M. | Merritt, C.R. | Rashti, M.R. | Singh, B. | Boyd, S.E. | Srivastava, P. | Brown, C.L. | Butler, O.M. | Kookana, R.S. | Chen, C.
Available online 9 November 2016 | Abstract not available | Maryam Esfandbod, Christopher R. Merritt, Mehran Rezaei Rashti, Balwant Singh, Sue E. Boyd, Prashant Srivastava, Christopher L. Brown, Orpheus M. Butler, Rai S. Kookana, Chengrong Chen
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