A bioassay method using the early life stages (germlings) of macroalgae was developed to detect toxicity of anti-fouling paint biocides. A laboratory based bioassay using Ulva intestinalis and Fucus spiralis germlings was performed with 4 common anti-fouling biocides (tributyltin (TBT), Irgarol 1051, Diuron and zinc sulphate), over a range of environmentally relevant concentrations (0.0033–10μgl−1). Comparison between the two species showed that germlings of U. intestinalis were better adapted for in-situ monitoring, as germlings of F. spiralis appeared to be too robust to display sufficient growth differences. The response of U. intestinalis germling growth appeared to reflect environmental biocide concentrations. Overall the developed method showed potential for the assessment of the sub-lethal effects of anti-fouling biocides on the early developmental stages of U. intestinalis.

This study classifies Mediterranean marine protected areas (MPAs) according to the combined result of pressure level and protection. Six major marine environment pressures were considered: pressures from fish farms, fishing, marine litter, pressures from marinas, pollution from maritime transport, and climate change. MPA protection was assessed through legal protection and management effort. Most MPA area in the Mediterranean is under relatively high pressure level and afforded low protection. Inshore areas show higher pressure levels. Five marine ecoregions, nine countries and nineteen MPA designation categories have over 50% of their MPA area under major concern. The mean number of cumulative pressures occurring in priority MPAs ranges between three and four, although the mean combined intensity of those pressures is low. However, these figures are most likely underestimated, especially for the southern Mediterranean. The most concerning pressures to MPAs regarding extent and intensity were: climate change, fishing and pollution from maritime transport.

An important priority for any organism is to maintain internal cellular homeostasis including acid–base balance. Yet, the molecular level impacts of changing environmental conditions, such as low pH, remain uncharacterised. Herein, we isolate partial Na+/H+exchangers (NHE), carbonic anhydrase (CA), and calmodulin (CaM) genes from a polychaete, Platynereis dumerilii and investigate their relative expression in acidified seawater conditions. mRNA expression of NHE was significantly down-regulated after 1h and up-regulated after 7days under low pH treatment (pH 7.8), indicating changes in acid–base transport. Furthermore, the localisation of NHE expression was also altered. A trend of down regulation in CA after 1h was also observed, suggesting a shift in the CO2 and HCO3− balance. No change in CaM expression was detected after 7days exposure to acidified seawater. This study provides insight into the molecular level changes taking place following exposure to acidified seawater in a non-calcifying, ubiquitous, organism.

A mathematical model was developed to simulate the oxidation of total sulfite in the holding tank of ammonia-based flue gas desulfurization (FGD) system. The model could provide predictions of the oxidation ratio of total sulfite at different operating condition such as pH, concentration of total sulfur, air flow and residence time. The calculation results of an ammonia-based FGD system for 2 × 150 MW boilers by this model were compared to that of corresponding measured values. The comparison results show that the simulated oxidation ratio of total sulfite agrees well with the measured data. The difference between calculated values and measured data is below 4.2%. This model appears to be beneficial for optimizing design and operation of total sulfite oxidation of an ammonia-based FGD system.

Fish species have attracted considerable interest in studies assessing biological responses to environmental contaminants. In this study, the attention has been focussed on fishbone of selected fish species from a highly polluted marine area, Augusta Bay (Italy, Central Mediterranean) to evaluate if toxicant elements had an effect on the mineralogical structure of bones, although macroscopic deformations were not evident. In particular, an attempt was made to evaluate if bone mineral features, such as crystallinity, mineral maturity and carbonate/phosphate mineral content, determined by XR-Diffraction and FT-IR Spectroscopy, suffered negative effects due to trace element levels in fishbone, detected by ICP-OES. Results confirmed the reliability of the use of diffractometric and spectroscopic techniques to assess the degree of crystallinity and the mineral maturity in fishbone. In addition, in highly polluted areas, Hg and Cr contamination induced a process of premature aging of fishbone, altering its biochemical and mineral contents.

The influence of wind direction and speed on the transport of vehicular air pollutants was investigated using a mobile laboratory in an urban area. We considered three spatial scales covering typical transport phenomena observed in urban areas: micro-scale (a few tens of meters), middle-scale (a few hundred meters) and neighborhood-scale (a few kilometers). Particle-bound polycyclic aromatic hydrocarbons (PAHs) were selected as an indicator of vehicle emissions in the roadway environment. From the micro-scale measurements, we found a significant difference in the concentration of particle-bound PAHs within a small spatial area, on a two-way road, due to the effect of wind direction when the prevailing wind direction was constant. In the middle-scale experiments, an exponential decrease in the concentration of particle-bound PAHs was observed with distance from the intersection in the direction of the prevailing wind, which is consistent with the results of previous studies. The concentration of particle-bound PAHs near the road was ∼10 times higher than that at 200 m away from the road due to the significantly low background concentration, indicating that particle-bound PAHs are a good indicator of vehicle emissions in a roadway environment. From long-distance (∼15 km) traveling measurements made over 14 days, we found that the average concentration of particle-bound PAHs on the road decreased as the prevailing wind speed increased at the neighborhood-scale, when the wind speed was higher than 2.0 m/s. This analysis, over three spatial scales, will be helpful in understanding the effect of wind on air pollution in a roadway environment in urban areas.

Aerosols released from biomass burning affect the tropospheric chemistry, radiation budget and cloud processes and hence can cause significant climate modifications. Due to certain economical reasons, the open burning of crop residue has become popular in Pakistan. In the present work we have analyzed the optical and physical properties of aerosols during crop residue burning over Lahore, a central location of Pakistan. The data from ground based Aerosol Robotic Network (AERONET), satellite based MODIS and CALIPSO remote sensing instruments have been used for the characterization of aerosols during crop residue burning event of October 2010. The maximum value (2.75) of daily mean AOD was observed on 20 October 2010 and the next highest value of 2.64 was observed on 19 October 2010, indicating heavy aerosol loading over Lahore on both days due to intense crop residue burning. The fine mode AOD values ranged from 0.14 to 2.68 (on 20 October 2010) with average value of 0.87 during October 2010 over Lahore. It was found that fine mode aerosols have greater contribution than coarse mode aerosols towards total aerosol burden indicating the presence of fine mode (crop residue burning) aerosols over Lahore. Cluster analysis showed that the mixed aerosols (biomass burning and urban-industrial) were present during the heavy aerosol loading period over Lahore. The highest volume concentration of fine mode occurred on 19 and 20 October 2010 representing the dominance of fine mode aerosols. Due to scattering of incoming solar radiation by intense smoke observed on 19 and 20 October 2010 high values of SSA (∼0.95) were found. HYSPLIT model backward trajectories showed that the winds transported aerosols from southeast and northwest directions.

Sterols and hydrocarbons were determined in the surface sediments from the transitional environment between Paranaguá Bay and the shallow continental shelf in the South Atlantic to assess the sources of organic matter (OM) and the contamination status of an area exposed to multiple anthropogenic inputs. Total aliphatic hydrocarbon concentrations were less than 10μgg−1, which is typical of unpolluted sediments, and related to recent inputs from higher terrestrial plants. Total polycyclic aromatic hydrocarbon ranged from<DL to 14.41ngg−1 (dry weight), which was predominantly derived from combustion with non-detectable levels occurring in 65% of the samples. Sterols typically related to marine sources predominated in the analysed sediments. Hence, the study area was protected from human activity. The relative absence of anthropogenic input and OM preservation clearly indicate that the organic markers analysed can be used to investigate the biogenic input of sedimentary OM in the study area.

Persistent organic pollutants (POPs), including polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs), are frequently detected in biota from Antarctica, whereas no data are available for their replacements, such as novel flame retardants (N-FRs). This study presented the occurrence of several N-FRs, PBDEs, and PCBs in tissue samples of an Antarctic rock cod (Trematomus bernacchii), a young gentoo penguin (Pygoscelis papua), and a brown skua (Stercorarius antarcticus) collected from King George Island. The total concentrations of N-FRs (ΣN-FRs; mean: 931pg/g dry weight (dw)) were comparable to PBDEs (Σ8PBDEs; 681pg/gdw), which were much lower than PCBs (ΣDL-PCBs; 12,800pg/gdw). Overall, skua contained two to three orders of magnitude higher contamination than penguin and fish. In the future, more attention should be focused on the fate of N-FRs in Antarctica, where usages have increased since PBDEs were banned. To our knowledge, this is the first report of N-FRs in biota from Antarctica.