Cu nanoparticles (CuNPs) have been widely used in numerous products, and may become a potential threat to marine organisms, but their behavior in the marine environments and potential toxicity to marine organisms remain little known. In the present study, we investigated the behavior of CuNPs in seawater, as well as the toxicity and bioaccumulation of CuNPs and copper sulfate (CuSO4) in barnacle larvae (Balanus amphitrite), a dominant fouling invertebrate in marine environment. CuNPs tended to aggregate in natural seawater and released Cu ion rapidly into seawater. The aggregation and release were especially higher at a lower concentration of CuNPs, e.g., 94–96% of CuNPs were released as Cu ions at 20 μg/L after 24 h. The larger size of CuNPs (40 nm) tended to display a higher solubility than the 20 nm CuNPs did. Humic acids enhanced the aggregation and inhibited the dissolution of CuNPs, and had a protective effect on the survival of nauplii II at higher Cu concentrations (100–200 μg/L). Comparison of the lethal concentrations showed that CuNPs were generally less toxic to the two stages of barnacle larvae (nauplii II and VI) than the Cu ions. The calculated 48-h LC50 values for nauplii II were 189.5 μg/L, 123.2 μg/L, and 89.8 μg/L for 20 nm CuNPs, 40 nm CuNPs, and CuSO4, respectively. However, the lethal concentrations of Cu bioaccumulation in the barnacle larvae were comparable between CuNPs and Cu ions when expressed by the actual tissue Cu bioaccumulation. Barnacle larval settlement decreased with an increase of Cu concentrations of both CuNPs and CuSO4, and was significantly inhibited at 100 μg/L CuSO4 and 150 μg/L CuNPs. Our results indicated that the toxicity of CuNPs could not be solely explained by the released Cu ions, and both CuNPs and the released Cu ion contributed to their toxicity and bioaccumulation in barnacle larvae.

Per- and polyfluoroalkyl substances (PFASs) are a large group of chemicals and can be detected in environmental and human samples all over the world. Toxicity of existing and emerging PFASs will be a long-term source of concern. This study aimed to investigate structure-dependent inhibitory effects of 14 PFASs towards the activity of 11 UDP-glucuronosyltransferase (UGT) isoforms. In vitro UGTs-catalyzed glucuronidation of 4-methylumbelliferone (4-MU) was employed to determine the inhibition of PFASs towards different UGT isoforms. All the PFASs showed <75% of inhibition or stimulation effects on UGT1A3, UGT1A7, UGT1A9, UGT2B4, UGT2B7 and UGT2B17. However, PFASs showed broad inhibition on the activity of UGT1A1 and UGT1A8. The activity of UGT1A1 was inhibited by 98.8%, 98%, 79.9%, 77.1%, and 76.9% at 100 μmoL/L of perfluorodecanoic acid (PFDA), perfluorooctanesulfonic acid potassium salt (PFOS), perfluorotetradecanoic acid (PFTA), perfluorooctanoic acid (PFOA) and perfluorododecanoic acid (PFDoA), respectively. UGT1A8 was inhibited by 97.6%, 94.8%, 86.3%, 83.4% and 77.1% by PFDA, PFTA, perfluorooctadecanoic acid (PFOcDA), PFDoA and PFOS, respectively. Additionally, PFDA significantly inhibited UGT1A6 and UGT1A10 by 96.8% and 91.6%, respectively. PFDoA inhibited the activity of UGT2B15 by 88.2%. PFDA and PFOS exhibited competitive inhibition towards UGT1A1, and PFDA and PFTA showed competitive inhibition towards UGT1A8. The inhibition kinetic parameter (Kᵢ) were 3.15, 1.73, 13.15 and 20.21 μmoL/L for PFDA-1A1, PFOS-1A1, PFDA-1A8 and PFTA-1A8, respectively. The values were calculated to be 0.3 μmoL/L and 1.3 μmoL/L for the in vivo inhibition of PFDA towards UGT1A1-and UGT1A8-catalyzed metabolism of substances, and 0.2 μmoL/L and 2.0 μmoL/L for the inhibition of PFOS towards UGT1A1 and the inhibition of PFTA towards UGT1A8, respectively. Molecular docking indicated that hydrogen bonds and hydrophobic interactions contributed to the interaction between PFASs and UGT isoforms. In conclusion, exposure to PFASs might inhibit the activity of UGTs to disturb metabolism of endogenous compounds and xenobiotics. The structure-related effects of PFASs on UGTs would be very important for risk assessment of PFASs.

Artificial light at night (ALAN) has become increasingly recognized as a disruptor of the reproductive endocrine process and behavior of wild birds. However, there is no evidence that ALAN directly disrupt the hypothalamus-pituitary-gonadal (HPG) axis, and no information on the effects of different ALAN intensities on birds. We experimentally tested whether ALAN affects reproductive endocrine activation in the HPG axis of birds, and whether this effect is related to the intensity of ALAN, in wild tree sparrows (Passer montanus). Forty-eight adult female birds were randomly assigned to four groups. They were first exposed to a short light photoperiod (8 h light and 16 h dark per day) for 20 days, then exposed to a long light photoperiod (16 h light and 8 h dark per day) to initiate the reproductive endocrine process. During these two kinds of photoperiod treatments, the four groups of birds were exposed to 0, 85, 150, and 300 lux light in the dark phase (night) respectively. The expression of the reproductive endocrine activation related TSH-β, Dio2 and GnRH-I gene was significantly higher in birds exposed to 85 lux light at night, and significantly lower in birds exposed to 150 and 300 lux, relative to the 0 lux control. The birds exposed to 85 lux had higher peak values of plasma LH and estradiol concentration and reached the peak earlier than birds exposed to 0, 150, or 300 lux did. The lower gene expression of birds exposed to 150 and 300 lux reduced their peak LH and estradiol values, but did not delay the timing of these peaks compared to the control group. These results reveal that low intensity ALAN accelerates the activation of the reproductive endocrine process in the HPG axis, whereas high intensity ALAN retards it.

Rapid industrialization has led to high levels of mercury (Hg) releases from anthropogenic sources in China. When deposited to terrestrial ecosystems, Hg has a high affinity for natural organic carbon. This means that Hg concentrations will vary naturally as a function of the total organic carbon (TOC) content of different soils and sediment. Thus, Hg to TOC ratios in topsoil and surface sediment provides a useful normalized tracer of the anthropogenic impact on Hg contamination. We compiled literature-documented Hg and total organic carbon (TOC) data for topsoil (n = 957) and surface sediment (n = 1142) in China. Topsoil samples (n = 100) were also collected in this study to broaden the spatial coverage. We found large differences in Hg:TOC ratios among topsoil from background sites, agricultural and urban areas, and mining sites and surface sediment from fluvial, coastal, and marine environments. Specifically, a significant increase in Hg:TOC ratios occurred between soils from background sites (median: Hg:TOC = 21.1; Inter-Quartile Range (IQR): 9.67 to 40.7) and agricultural areas (median: 34.1; IQR: 22.1 to 58.7), urban areas (median: 62.1 ng g−1; IQR: 34.2 to 154) and mining sites (median: 2780; range: 181 to 43500). Urban and mining sites show the largest increase in Hg:TOC ratios, reflecting elevated anthropogenic Hg inputs in these areas. Fluvial sediment showed higher Hg:TOC ratios (median: 197; IQR: 109 to 389) than coastal (median: 88.3; IQR: 46.8 to 168) and marine sediment (median: 89.7; IQR: 53 to 138), indicating decreased anthropogenic Hg input from rivers to coastal and marine regions. Results of our study suggest Hg:TOC ratios are a useful normalized indicator of the influence of anthropogenic Hg releases on Hg enrichment in topsoil and surface sediment.

Microplastics (particle size <5 mm) are an emerging contaminant for aquatic environmental, which have attracted increasing attention in worldwide range. In this study, an improved fluorescent staining method for detection and quantification of microplastics was developed based on thermal expansion and contraction. This method is effective in detection of polyethylene, polystyrene, polyvinyl chloride and polyethylene terephthalate plastic particles. In order to avoid error statistics caused by pretreatment, various characterizations of microplastics were measured after heated, such as microstructure, compositions and thermostability. The results showed that there was no significant damage to microplastics even under heating condition at 75 °C for 30 min, and the stained microplastics had strong stability for up to two months. Moreover, this method has been successfully applied to the quantification of microplastics in biological samples and result showed there were about 54 particles g⁻¹ (dry weight) microplastics in the Sipunculus nudus. This new method provides a reliable method for quantitative analysis of microplastics in environment and biological tissue.

Metals in indoor dust pose potential health risks to humans. Dust deposition on air conditioner filters can represent the resuspended particulate matter in indoor air. However, few studies have examined this until now. This study investigated the total concentrations and different chemical fractionations of Cd, Cr, Mn, Ni, Pb, Sb, V, and Zn in indoor dust from three different functional zones (the Chief District, Commercial District (CmD), and Industrial District) in Hefei. The mean metal concentrations in indoor dust decreased in the following order: Zn > Mn > Pb > Cr > Ni > V > Cd > Sb. Cd, Pb, and Zn mainly existed in the mobile fraction. Cr and V mainly existed in the residual fraction. The enrichment factor and geo-accumulation index values of heavy metals were all ranked in the order of Cd > Zn > Pb > Sb > Ni > Cr > V, and these values in indoor dust were larger than those in outdoor dust. In addition, the enrichment patterns of these elements were similar in the three functional areas. The orders of non-carcinogenic risk (hazard index; HI) for the different functional areas for children were roughly the same, but there were clear differences for adults. In general, all the HIs were less than 1, which were within the internationally recognized safe range. The total carcinogenic risk (TR) was in the order of Cr > Pb > Cd for both children and adults in the three functional zones. The TRs from Cr exposure were not negligible. The TRs were significantly higher in the CmD.

Microplastics (MPs) in the environment have become an issue worldwide. However, data about MPs in freshwater systems are still limited so far. This study investigated sources, fate, and seasonal and spatial distribution of MPs in the main stream Pearl River and its tributaries, as well as in the Pearl River Estuary (PRE), China. MPs were widely detected in the river water, river bed sediment, and estuarine sediment, with abundances of 0.57 ± 0.71 items L⁻¹, 685 ± 342 items kg⁻¹ dry weight (dw), and 258 ± 133 items kg⁻¹ dw, respectively. Sheet, fragmental, and fibrous polyethylene, polypropylene, and ethylene-propylene copolymers were predominant, suggesting that MPs in the Pearl River catchment be mainly derived from fragmentation of discarded plastic wastes. In addition, municipal wastewater was also an important MPs source, especially for polyethylene terephthalate (PET) fibers. Polymers of higher density, such as PET and polyvinyl alcohol were relatively more abundant in the sediment than in the river water, especially in the estuarine sediment. Upward increase of the MP abundance was observed in the sedimentary core, probably indicating increasing release of plastic wastes due to growing production and uses of plastic products. On the other hand, percentage of finer MPs increased with increasing depth. The results revealed persistence and potential downward dispersion of the fine MPs. The MPs abundance was positively related with population density and gross domestic product, demonstrating impacts of human activities and economic development on the MPs contamination. Higher MPs abundance was detected in dry season than in wet season in the river water, suggesting dilution effect of precipitation. It's estimated that 15963 tons of MPs could be released annually into the PRE from the main stream Pearl River and its tributaries.

Di(2-ethylhexyl) phthalate (DEHP), as a widely used plasticizer, is reported to have widespread environmental and global health hazards. Trace amounts of phthalates in the environment are sufficient to disrupt ecological balance and affect human health. However, DEHP-induced splenic toxicity remains in an unknown state. Therefore, to explore the mechanism of DEHP-induced splenic toxicity, male quail were employed with 0, 250, 500 and 750 mg/kg body weight DEHP by daily gastric perfusion for 45 days. Notably, splenic corpuscular border and cell gap enlargement were observed in the spleen tissue of DEHP-exposed quail under the histopathological analysis. Furthermore, DEHP induced dysregulation of oxidative stress markers by increasing malondialdehyde (MDA) content and decreasing superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities. Low concentration of DEHP (≤250 mg/kg) exposure suppressed nuclear factor-E2-related factor 2 (Nrf2) signaling pathway, while high concentration of DEHP (≥500 mg/kg) exposure activated Nrf2-mediated defense response. DEHP induced splenic oxidative stress via interfering Nrf2 signal pathway and altering the transcription of its downstream genes. In conclusion, this study suggested that DEHP induced splenic toxicity.

Carbonaceous aerosols (CAs) scatter and absorb incident solar radiation in the atmosphere, thereby influencing the regional climate and hydrological cycle, particularly in the Third Pole (TP). Here, we present the characteristics of CAs at 19 observation stations from the Atmospheric Pollution and Cryospheric Change network to obtain a deep understanding of pollutant status in the TP. The organic carbon (OC) and elemental carbon (EC) concentrations decreased noticeably inwards from outside to inland of the TP, consistent with their emission load and also affected by transport process and meteorological condition. Urban areas, such as Kathmandu, Karachi, and Mardan, exhibited extremely high OC and EC concentrations, with low and high values occurring in the monsoon and non-monsoon seasons, respectively. However, remote regions inland the TP (e.g., Nam Co and Ngari) demonstrated much lower OC and EC concentrations. Different seasonal variations were observed between the southern and northern parts of the TP, suggesting differences in the patterns of pollutant sources and in distance from the sources between the two regions. In addition to the influence of long-range transported pollutants from the Indo-Gangetic Plain (IGP), the TP was affected by local emissions (e.g., biomass burning). The OC/EC ratio also suggested that biomass burning was prevalent in the center TP, whereas the marginal sites (e.g., Jomsom, Dhunche, and Laohugou) were affected by fossil fuel combustion from the up-wind regions. The mass absorption cross-section of EC (MACEC) at 632 nm ranged from 6.56 to 14.7 m² g⁻¹, with an increasing trend from outside to inland of the TP. Urban areas had low MACEC values because such regions were mainly affected by local fresh emissions. In addition, large amount of brown carbon can decrease the MACEC values in cities of South Asia. Remote sites had high MACEC values because of the coating enhancement of aerosols.Influenced by emission, transport process, and weather condition, the CA concentrations and MACEC presented decreasing and increasing trends, respectively, from outside to inland of the TP.

Employing biochar for environmental remediation has been widely practiced. Nonetheless, the reduction mechanisms of hexavalent chromium (Cr(VI)) in the presence of biochar have not been fully elucidated (i.e., direct or indirect reduction of Cr(VI) by biochar). In particular, the effect of light on Cr(VI) reduction by biochar was rarely reported. Thus, to clarify the reduction mechanisms of Cr(VI) by biochar at the fundamental level, this study laid great emphasis on the photo-induced reduction of Cr(VI) in the application of biochar. Biochar releases dissolved organic matter (DOM), the DOM can extract Fe(III) from soil by complexation, and the complexes can be photo-reacted under the light. In these respects, Fe(II) formed by the photo-induced reaction of DOM-Fe(III) was particularly evaluated in this study. To evaluate that, three biomass samples (rice straw, granular sludge from an up-flow anaerobic sludge blanket, and spent coffee ground) were torrefied to biochar. To circumvent the adsorption of Cr(VI) onto biochar, biochar extractives (served as a source for DOM) and Fe(III) solution were tested with/without UV light to prove Fe(II) formation. This study experimentally proved that the more Fe(II) under the UV radiation was formed in the co-existence with biochar extractives and Fe(III). All experimental data from three biochar samples were indeed very similar. Cr(VI) reduction by Fe(II) from GB, RB, and CB reached up to 96, 79, and 100%, respectively. The different reduction efficiency signified that the low molecular weight of organic acids, such as oxalate, were more sensitive to the UV light, thereby resulting in the enhanced Fe(II) formation. Such Fe(II) formation subsequently led to the high reduction efficiency of Cr(VI).