Nowadays one of most urgent problems is the production of healthy food which ensures normal maintenance of the human body. Rye bread is one of the bases of wholesome food. Every year scientists in Europe and other countries carry out investigations on the nutritional impact of processing rye. Interesting questions arise regarding changes in the biologically active compounds in rye bread as a result of processed. Connate antiradical activity (ARA) of rye bread affects free radicals by scavenging or donating electrons, which suggests that the ARA of rye bread averts attacks of free radicals on human cells. Therefore it is important to use antioxidants containing products with antiradical activity in human diets. The aim of this study was to analyse the impact of baking processing on rye bread antiradical activity. The object of the study was Latvian traditional rye bread baked in a commercial bakery, made with scald, and baked in a clay-floor firewood oven. The antiradical activity of rye bread crumb and crust was measured by using free radical 2.2-diphenyl-1-picrylhydrazyl (DPPH). It was concluded in the research that rye flour type 1740 has antiradical activity by scavenging 67 μmol·100gE-1 DM of the stable free radical DPPH, which is 13% more than the dough antiradical activity of the same flour. The antiradical activity of the crust is 49.6% more than the antiradical activity of the crumb. Baking time has no significant impact on changes in the antiradical activity of rye bread crumb.

Cereal grains have long been thought to be less important sources of antioxidants than fruits and vegetables although they contain many antioxidants and are major dietary components worldwide. The aim of the current research was to study and compare an antioxidant activity (AOA) and total phenolic content (TPC) of different barley varieties and malt types as well as to evaluate possible interconnection between TPC and AOA of barley and malt samples. The research was carried out on four lines of hull-less barley ‘3528’; ’L-400’; ’3475’; ‘3537’ and one variety of flaky barley ‘Klass’ grains, which were cultivated in Latvia in 2010, and their corresponding malt. Commercial sorts of malt - Pilsener, Munich, Caramel and Dark were used in the research to compare with the malt produced in the laboratory scale. The antioxidant potential of barley and their products is analyzed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. Total phenolic content of barley and malt samples was determined according to the Folin-Ciocalteu spectrophotometric methods. The values of DPPH radical scavenging activity for 5 barley samples ranged from 4.57 to 5.89 μmol TE gE-1 DW. The total amount of phenols ranged from 1.96 to 2.43 mg GAE gE-1 DW for unprocessed barley samples and from 2.5 to 3.4 mg GAE gE-1 DW for their corresponding malt. TPC of malt commercial sort ranged from 3.5 to 6.7 GAE gE-1 DW. The increase of TPC for commercial malts is strongly related with Maillard reaction products.

Horseradish (Armoracia rusticana L.) is a perennial plant, with a particularly pungent flavour and significant antioxidant properties. The aim of current research was to determine the total phenol content and antioxidant properties of horseradish depending on genotype and harvest time. For experiments nine genotypes of horseradish roots collected at different times were investigated. Fresh plant material was extracted with ethanol/water solution (80:20 v/v). Total phenols content (TPC) of plant extracts was determined according to the Folin-Ciocalteu spectrophotometric method and results were expressed as gallic acid equivalents (GAE). Antioxidant activity of the extracts was measured on the basis of DPPH˙ free radical scavenging activity and the final results were expressed as inhibition of 2,2-diphenyl-1- picrylhydraziyl (DPPH˙) in percent (I, %). Total phenols content varied among analysed types of horseradish. The highest TPC was detected in horseradish root genotype 280 harvested in September and it also demonstrated the highest DPPH˙ radical scavenging activity, whereas the lowest TPC was detected in horseradish root genotype 26B also harvested in September. TPC and DPPH˙ scavenging antioxidant activity were also significantly influenced by harvest time. Positive correlation was found between antiradical activity and the total phenols content in horseradish roots harvested in September. In further experiments, use of horseradish as natural antioxidants in different food matrixes should be studied.

Fruits and vegetables are a major source of antioxidants. The aim of current research was to study and compare the antiradical activity, the total polyphenol content (TPC) and the total flavonoid content (TFC) in dried carrots (Daucus carota), pumpkins (Cucurbita maxima), leeks (Allium ampeloprasum var. porrum) and black radish (Raphamussativus) using a traditional convective drier and a microwave-vacuum drier. For each vegetable steaming as pre-treatment was used. Vegetables were harvested in Latvia in 2012, gathered when ripe and then dried. Analyses were made in Latvia University of Agriculture, Faculty of Food Technology laboratories. The total polyphenol content was determined by the Folin- Ciocalteu method and the total flavonoid content - using spectrophotometric method. The antiradical activity was analyzed by the 2,2-diphenyl-1-picrilhydrazyl (DPPH) assay. The results of experiments demonstrate that the total amount of polyphenols ranged from 98.97 to 623.70 mg gallic acid equivalent (GAE) 100 gE-1 in dry weight and the total amount of flavonoids ranged from 40.32 to 100.23 mg catechin equivalent (CE) 100 gE-1 in dry weight. The value of DPPH antiradical activity for vegetable samples ranged from 6.10 to 45.14 percents.

Horseradish (Armoracia rusticana L.) is a perennial herb belonging to the Brassicaceae family and contains biologically active substances. The aim of the current research was to determine the most suitable extraction method and solvent for obtaining horseradish leaf extracts with high antiradical activity. For experiments fresh leaves of horseradish were extracted with seven different solvents: n-hexane, diethyl ether, 2-propanol, acetone, ethanol (95%), ethanolwater/acetic acid (80/20/1 v/v/v) and ethanol / water (80/20 by volume) using two extraction methods (conventional and Soxhlet). For all extracts, total phenolic, flavonoid content and DPPH˙, ABTS radical scavenging activity, and reducing power were determined using a spectrophotometric method. As the best solvent can be used ethanol. Total phenolic content and total flavonoid content was higher in Soxhlet extracts. Comparing to other plants, the proportion of flavonoids in the amount of total phenolics is average, and it increases by increasing the polarity of used solvent. It can be concluded that by using Soxhlet extraction method it is possible to obtain extracts that are effective antioxidants. A very strong and a strong correlation has been identified between levels of phenolic compounds and antioxidant capacities of the extracts.

The quality and nutritional value of milk and dairy products are considerably influenced by the stability of its constituents. The aim of the present study was to evaluate the possibility of enhancing oxidative stability of cow’s milk fat and vitamin B2 using carrots and palm oil feed supplement CAF 100 as sources of natural antioxidants in cow feed. Milk samples were collected after 25-day period of feed supplementation. The intensity of riboflavin losses during its photo oxidative degradation in sunlight was measured by the fluorometric method. A slight and significant (p is less than 0.05) difference in higher vitamin B2 stability was seen in carrot-supplemented group (TG1) milk, where the losses of vitamin B2 were by 3.01% less compared to CG (CG). The oxidative stability of butter oil samples stored in 60 °C temperature was analyzed by peroxide value (PV) method. The oxidative stability of samples initially affected by light from both trial groups was significantly (p is less than 0.05) higher compared to CG showing the good potential of the cow diet enrichment with carotenoid additives. The longest induction period (larger than 14 days) was observed in CAF-100- supplemented (TG2) cow milk fats, which can be explained by carotenoids and tocopherol presence and its possible synergism in fat protection. The induction periods of the TG1 and CG were 12.03 and 10.97 days, respectively.

β-Carotene (BC) and a-tocopherol in milk fat have positive implications in human nutrition, besides the specifically protecting polyunsaturated fatty acids from oxidation. To determine the possible effect on some milk components, nutritional value and lipid stability of including carrots in the diet of lactating cows, ten cows were selected in a dairy farm and divided into 2 groups (control and experimental) by 5 cows in each. In experimental group's diet additionally 7 kg carrots per cow per day were included. Individual cow milk samples were obtained 1 day before feed enrichment, in days 7, 24, 35, 42 (during the feed enrichment), and 1 week after feed enrichment. Analyses of milk fat, protein content, somatic cell count (SCC), retinol, BC, vitamin C, tocopherols, immunoglobulins (Ig), lysozyme, fatty acids (FA) were made, and milk yield was measured. Supplying cow diet with carrots showed a tendency to improve milk quality by promoting the faster decrease of SCC, and significantly (p is less than 0.05) increasing Ig and lysozyme content, thus potentially improving milk nutritional value. The vitamin content rose significantly (p is less than 0.05) by retinol and tocopherol contents, but b-carotene content increase in milk was not observed. Also milk fatty acid (FA) stability changes during 5 day storage in temperature of 4 - 6 deg C were not observed.

Vegetable processing in food industry results in significant number of by-products – peel, mark, bark, seeds still rich in bioactive compounds. The objective of this study was to investigate the stability of total phenolic content (TPC) and antioxidant activity after high temperature short time (HTST) extrusion cooking of a rye and oat- based matrix with addition of apple, carrot and pumpkin by-product flour (BPF) in various amounts (5%, 10%, 15% and 20%). Extrusion was performed with co-rotating twin-screw extruder (compression ratio 8:1) at Ltd MILZU. The main drive of extruder was provided with a 7.5 HP motor (400 V, 3 HP, 50 cycles). Temperatures for extrusion zones were 125 °C /135 °C /145 °C. TPC was determined using the Folin Ciocalteu method. Antioxidant activity was evaluated by free radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant scavenging activity. TPC in cereal-based crispbread was 62.03 ± 0.15 mg GAE gE-1 DW before extrusion and 37.73 ± 1.96 mg GAE gE-1 DW after extrusion. Addition 20% of apple BPF increased TPC in crispbread to 193.92 ± 1.37, carrot BPF 171.36 ± 6.97 and pumpkin BPF to 195.09 ± 4.68 mg GAE gE-1 DW after extrusion. Antioxidant activity of control (20% oats, 80% rye flour blends) sample was 0.516 ± 0.192 mg TE gE-1 DW but in samples with addition of 20% by-products, it was significantly higher reaching 0.926 ± 0.05 TE gE-1 DW in samples with pumpkin by-products after extrusion.

The aim of this study was to investigate the effect of industrially designed light-emitting diode (LED) lamp lighting on the nutritional quality of Brassicaceae microgreens. Red pak choi (Brassica rapa var. chinensis ‘Rubi F1 ’), tatsoi (Brassica rapa var. rosularis) and mustard (Brassica juncea L. ‘Red Lion’) were grown in a greenhouse (20±2/18±2 °C) during winter season, and the solar daily integral (DLI) was ~3.46±1.16 mol mE-2 dE-1. The light spectra of lamp consist of 8 violet (420-430), 16 blue (460-470 nm), 8 orange (610-615 nm), 3 red (620-630 nm), 56 red (660-670 nm), 8 white (contain blue (400-500 nm), green (500-600 nm) and red (600-700 nm)) LEDs. The treatments of ~150 and ~250 µmol m-2 sE-1 LED irradiance levels (LED 150 and LED 250) for 16 h dE-1 in comparison with high pressure sodium (HPS) lamps (~150 µmol mE-2 sE-1) as a control were performed. Photophysiological response to the artificial light varied among Brassicaceae species. Microgreens treated with LED 150 and LED 250 were significantly (P is less than or equal to 0.05) shorter and formed smaller hypocotyls. The photooxidative changes were evoked by both lighting treatments and led to higher phytochemical (phenols, ascorbic acid, flavonols, anthocyanins) and mineral element (Ca, K, Mg, Na, P, Fe, Zn) contents, and the DPPH and ABTS free radicals scavenging activities in all microgreens. Significantly lower content of nitrate was obtained with LED 150 treatment. Finally, LED lamps have the potential to be used as the main light source for growing high nutritional quality microgreens in greenhouses.