Pyrenophora tritici-repentis is a major wheat pathogen in all wheat (Triticum spp.) growing areas worldwide. Up to date, eight P. tritici-repentis races have been described based on chlorosis, necrosis, or both symptoms caused on race differential wheat genotypes: ‘Glenlea’, 6B662, 6B365, and ‘Salamouni’. Symptom development on differential genotypes depends on the interaction of the pathogen’s necrotrophic effectors named Ptr ToxA, Ptr ToxB, and Ptr ToxC with host susceptibility genes. Ptr ToxA is encoded by the single copy gene ToxA and induces necrosis on sensitive wheat cultivars. Ptr ToxB causes chlorosis and is encoded by the multicopy gene ToxB. The Ptr ToxC is the non-proteinaceous, polar, low molecular mass molecule that also induces chlorosis, but up to date, the gene encoding this toxin is unknown. Races producing Ptr ToxA are predominant in the global Ptr population. There are several reports about new putative races of P. tritici-repentis that do not conform to the current race system, so further research is required. This study aims to collect and systematise available information about the virulence and races of P. tritici-repentis.

Tan spot is an economically important disease of wheat in Latvia, but there is still insufficient information about its causal agent’s Pyrenophora tritici-repentis diversity in Latvia. The aim of this study was to evaluate the disease phenotype in the field and the possible presence of necrotrophic effectors Ptr ToxA, Ptr ToxB and Ptr ToxC in the P. tritici-repentis population in Latvia. Typical differential wheat lines ‘Glenlea’, ‘6B662’, ‘6B365’ and ‘Salamouni’ were evaluated under the field conditions in 2023 in the central part of Latvia. The highest tan spot severity was observed during the flowering stage with no significant difference between wheat genotypes. During the experiment, the typical disease phenotypes corresponding to all three known effectors were noted. Additionally, typical tan spot symptoms were determined on the resistant cultivar ‘Salamouni’, confirming the presence of new, currently uncharacterised effectors that are not compatible with the existing race model. Future studies should focus on searching for new effectors and further characterising known necrotrophic effectors, as knowledge about P. tritici-repentis and wheat interaction is still incomplete.