The objective of the research was the shelf life extension of a salad with meat in mayonnaise. The microbial stability of the salad with meat in mayonnaise packaged under vacuum, in modified atmosphere, and using 'Sous vide' technology was examined and compared with conventionally packaged (in ambient air) salad. The salads were packaged in polyamide/polyethylene pouches with barrier properties as well as in pouches made from biodegradable plasticized poly-β-hydroxybutyrate or polylactic acid film. Control samples without preservatives were packed in traditional polypropylene containers covered with non-hermetic lids. PURASA L Powder Opti Form (sodium lactate) in the amount 5 g kgE-1, 10 g kgE-1, and 15 g kgE-1, and ethyl alcohol in the amount of 2 ml were used as single preservatives for separate samples. The studies of the samples were carried out after 1, 3, 7, 10, 15, 18, 25, 29, 42, and 52 storage days at the temperature of +4+-0.5 deg C. Experimentally the quality of the salad with meat in mayonnaise was characterized by measuring the total plate count of microorganisms. The obtained results indicate that different packaging technologies and addition of preservatives show significantly different influence on the microbial growth in different samples. 'Sous vide' packaging technology was effective for the shelf life extending and guarantee of safety of the salads with meat in mayonnaise.

Investigations of textural parameters of vacuum, protective gas atmosphere (MAP), and at air ambiance packed fresh carp (Cyprinus carpio L.) farmed in Latvia were carried out. The samples of carp were stored at two different temperatures (0 +- 0.5 and +4 +- 0.5 ºC) applying three different types of packaging – vacuum, MAP, and wrapping at air ambiance. Gas mixture used for MAP consisted of 40% CO2 and 60% N2. Samples were analyzed before packaging (day 0) and after 2, 4, 6, 8, 11 and 14 storage days. Textural parameters – hardness and shear force were evaluated by texture analyzer TA.XT.plus (Stable Micro System Ltd.). Two instrumental methods were applied for evaluation of textural properties. One method was based on compression test, using spherical probe, 25.4 mm in diameter (type P/1S), and measuring the hardness of the fillet and other method was based on cutting the fillet with a blade (type HDP/BSK) and measuring the shear force. The instrumental hardness and shear force of fresh carp fillets decreased during storage time. Texture variables significantly correlate with storage time, respectively, r = -0.723 and r = -0.748, the hardness and shear force. The storage time, temperature and packaging type with probability of 95 % substantially influence the textural parameters of carp fillets (P is less than 0.05).

The aim of this study was to evaluate physical and chemical changes in sous vide cooked marinated parent stock hen breast meat during refrigerated storage. Sous vide method is suitable for preparation of ready-to-eat meat products satisfying consumer demand for convenience and safety. Meat tenderness is recognized as important quality attribute of meats. The study involved preparation of the raw material, vacuum packaging of the products in polyamide/polyethylene (PA/PE) film pouches, marinating for 20 hours, sous vide cooking and chilling in ice water, followed by storage at 3.0±0.5 deg C. Each sample containing a chicken portion or chicken mix with shredded carrots, sea buckthorn sauce and spices was analysed during storage. Water activity, pH, and moisture were assessed during storage. Hardness (shear force) measurement was used as an instrumental method for meat tenderness evaluation. The differences between two studied products were noted regarding all studied parameters – water activity, pH, moisture content, and hardness. The differences mainly were determined by the properties of carrot and sea buckthorn additive, which generally have higher moisture content and lower pH. The researched parameters were well preserved during refrigerated storage for 28 days.

The non-spore forming gram-positive bacterium Listeria monocytogenes is a food pathogen bacterium and a causative agent of listeriosis. The aim of the study was to determine the survival limits of L. monocytogenes inoculated in manufactured vacuum-packed cold smoked pork depending on shelf time, supported by water activity (aw) and pH values. Enumeration of L. monocytogenes colony forming units per gram (cfu gE-1) was done according to ISO standard. Water activity (aw) and pH values in pork samples were more or less constant and supported L. monocytogenes growth. The behaviour of L. monocytogenes in cold-smoked sliced pork by shelf time, when environmental factors changed minimally and supported growth, largely depended on the initial contamination level. A lag-phase of bacterial growing process before exponential growth rate of inoculated L. monocytogenes depended on initial cell concentration and had 10 days step level if storage temperature was approximately 5 deg C. A significant Pearson’s correlation (p is less than 0.01) was established between the microbiological test values of L. monocytogenes count changes in sliced and packed cold-smoked pork during storage time of 60 days. The main parameter which maintained negative polynomial growth rate of L. monocytogenes in cold-smoked pork was the decrease of live cell concentration in samples below lg 2.0. The experiments were done at the Faculty of Veterinary Medicine of Latvia University of Agriculture and at a sausage manufacturer’s laboratory.

Fresh meat is a highly perishable product due to its biological composition as it serves as an ideal environment for the growth and propagation of microorganisms and common food-borne pathogens. High pressure processing (HPP) is a cold pasteurization treatment to extend shelf-life while preserving the sensory and nutritional characteristics of the product. The aim of this research was to evaluate the effects of HPP on the fresh porcine Musculus longissimus lumborum microbial load and related physical properties (pH, water activity aw, and moisture content). Vacuum packed meat samples were treated at 50, 100, 200, 300, 400, and 500 MPa for 1, 5, and 15 min in a high-pressure processor ISO-Lab S-FL-100-250-09-W (Stansted Fluid Power Ltd., UK). Pressure treatment above 300 MPa resulted in a significant (p is less than 0.05) decrease of total plate count. However, the studied pressurizing time had no significant effect on microbial lethality at the same pressure applied. Other important parameters such as water activity, moisture, and pH were determined as they directly affect microorganism growth and resistance to pressure. A slight increase in pork pH was observed with increased pressure. No significant changes in water activity and moisture content were observed as a result of high pressure treatment. For future researches it would be important to evaluate the dynamics of microbial growth during storing as part of cells after pressure treatment are injured and not eliminated immediately; therefore, microbial count may further decrease during cold storage.

High-pressure processing (HPP) is typically used for the microorganism inactivation, which provides safety and prolonged shelf life of meat and meat products. However, for consumers along with safety, it is important to have good sensory properties, which is a combination of tender and juicy meat with an intense meat flavour. These attributes may change because of the high pressure processing; therefore, the aim of the study was to evaluate the effect of HPP on sensory and physical attributes of pork upon processing at 300 and 600 MPa at room temperature for 1 and 15 min. After HPP the processed pork samples were cooked within the package in a water bath. Colour of cooked pork did not differ among samples. Moisture content of samples decreased with the increased processing time. Sensory evaluation revealed that HPP treatment did not influence the colour and flavour of cooked pork irrespective of treatment parameters applied in the current study. The panellists indicated that increased pressure made pork samples drier and tougher, thus changing such sensory attributes as juiciness and chewiness, which are important for meat palatability. The correlation found between chewiness determined by sensory analysis and toughness determined by Warner-Bratzler shear device suggested this instrumental method as a better tool when compared to the instrumental texture profile analysis (TPA).