The objective of the study was to investigate the microbiological content of cow (Bos primigenius) milk in Latvian organic farms according to season and herd size with a purpose to detect their impact on the distribution of mastitis causing pathogens in milk. Samples were collected in 14 organic dairy farms of Latvia, 4 times through 2012: in winter, autumn, spring and summer period. Raw milk samples (n=564) obtained from cow composite milk were studied. The samples were divided into three groups in accordance with the number of cows in the cow-shed: A (3-30), B (31-60) and C (61-124). The total colony count (TTC) and the isolation of mastitis causing bacteria were analysed using standard methods. Bacterial growth occurred in 90.4% of samples. Isolated microorganisms belonged to 35 species, and the following bacteria were the most prevalent agent, including Coagulase negative staphylococci in 29.4%, Staphylococcus aureus in 24.1%, Kocuria kristinae in 12.9%, and Enterobacteriaceae spp. in 10.3% out of 564 milk samples. Depending on the season, the average number of TCC was the lowest in summer (4.66 ± 4.01 log10 CFU mLE-1), moderately higher in spring and winter (4.72 ± 4.18 and 4.82 ± 3.54 log10 CFU mLE-1, respectively), but significantly higher in autumn (5.43 ± 4.80 log10 CFU mL-E1). Coliforms were not isolated from group A herds instead of B (3.1%) and C (4.6%). Occurrence of Staphylococcus aureus was noticeably higher in B (19.1%) and C (20.8%) than in A (12.6%) herds. Most of group A herds met the milk quality requirements, while group C herds produced more contaminated milk.

The present study describes situation of antimicrobial resistance of animal pathogens and resistance trends in Estonia in years 2006-2009. Bacterial strains isolated during period 2006-2009 were Escherichia coli (E. coli), Enterococcus faecium (E. faecium), Enterococcus faecalis (E. faecalis), collected from healthy pigs faeces as well as from diagnostic submissions of pig samples. Staphylococcus aureus (S. aureus) isolates originated from cows with clinical mastitis and Staphylococcus pseudointermedius (S. pseudointermedius) isolates from dogs with pyoderma or otitis externa. Antimicrobial susceptibility was detected by microdilution method. Normal enteric microflora from health y pigs had resistance against streptomycin, tetracyclin, sulfametoxazol and trimethoprim. E. faecalis and E. faecium were resistant to erythromycin, tetracyclin, streptomycin and kanamycin. Multiresistance occured mainly against kanamycin, streptomycin and tetracyclin. E. coli strains isolated from pathological material showed high resistance to ampicillin, tetracycline, streptomycin, sulphonamides and trimethoprim. Multiresistance was detected between 60–73% during study years. In 2009, one ESBL (extended spectrum betalactamase) producing isolate was observed. S. aureus strains isolated from clinical mastitis samples were mainly penicillin resistant (58–86%). Meticillinresistant S. aureus was not found during the study. In 2009, resistance to lincomycin (30%) and fucidinic acid (22%) was detected. In S. pseudointermedius strains isolated from canine skin samples the prevalence of resistance to penicillin as high as 53–81% was found. Multidrug resistance was relatively stable being 38% in 2006, 29% in 2007 and 25% in 2009. In conclusion, antimicrobial resistance of animal pathogens in Estonia was high. Further improvement of prudent use of antimicrobials and infection control is needed.

The aim of investigations was to compare different absorber material efficiency to examine collector tracking the Sun and stationary collector efficiency as well as the efficiency of insulated and non-insulated collectors. The 0.1x0.5x1.0 meter long experimental solar collectors were constructed for investigations and different types of absorber materials were made. The manifold length, the Sun radiation effect on the degree of air heating was analyzed. ASHARE used standard 93-2003 is determined for insulated and non-insulated solar collectors and absorber effectiveness. We determined the influence of the Sun radiation on the air heating degree for those types of absorbers. The experimental data were measured and recorded in the electronic equipment REG (electronic metering and recording equipment of temperature, radiation and lighting). The collector covered material was a polystyrol plate and different absorbers. We compared insulated and non-insulated collectors to prove that the insulated collector is more effective. The insulated collector was made of the collector surfaces faced with cellular plastic two cm plates. Our task was to calculate the air heating solar collector efficiency. The collector tracking the Sun is approximately 30% more efficient than the same type of stationary collectors in operation time for 6 hours. Insulation increases solar panel efficiency especially in windy weather. The insulated collector demonstrates almost two times higher efficiency than the non-insulated collectors (up to 93%) with absorbent material steel tinplate on top.

This research was carried out to start the discussion on the amount of harmful emissions that are emitted in producing building and insulation materials for roof constructions. Usually, for the improvement of energy efficiency of buildings and reduction of the embodied thermal energy, effective thermal insulation solutions for the external building envelope have to be provided. From the buildings available for the analysis, in Latvia there were selected multi-apartment buildings of separate series with a uniform composition of roof constructions. Within the context of renovation works, the reports of energy audit for the buildings contain recommendations referred to improvement of the thermal performance of roofs. Using mutually comparable energy efficiency report data, there was drawn up an averaged model of a five-storey multi-apartment residential house. There were taken into account legislative documents relating to thermal engineering of buildings: LBN 002-01 ‘Heat engineering of building boundary constructions’ and LVS EN ISO 6946: 2007 ‘Building components and building elements – Thermal resistance and thermal transmittance’, and moisture regime according to LVS EN ISO 13788: 2001 ‘Hygrothermal performance of building components and building elements – internal surface temperature to avoid critical surface humidity and interspatial condensation – calculation methods’, which states that the roof constructions should be free from possibility of water vapour condensation. In this study, there were calculated energy savings obtained by improving thermal resistance of the roof constructions and the opposite primary energy consumption for the production of building materials. As a result, there is obtained environmentally friendly roof construction.

In Latvia, use of unheated cow sheds with lowered environmental temperature is growing. In such circumstances, cooling of the milking equipment washing liquid is more rapid and quality of the washing equipment decreases. However, it may be eliminated by coating the milk and washing liquid piping with heat insulation. A mathematical model of the milking equipment washing liquid by which the end temperature of the washing liquid cooling can be determined is developed. Two types of heat insulation materials are under the trial. Laboratory experiments on cooling of the washing liquid are carried out and the experimental coefficients of the mathematical model are determined.