The aim of the work is to investigate raw milk from different bulls' daughters to clear up the influence of cows' genetic predisposition to milk microbial contamination and somatic cell count (SCC). Particular attention is paid to develop productive cow milk yield, milk fat, milk protein and in recent years, the SCC heredity. The literature describes a variety of lines and families, representatives of the resistance to certain diseases (leucosis, mastitis, tuberculosis, brucellosis, foot disease), it shows the determination of a genetic factor for this trait. If the selection results show increased resistance to a disease, then this indicates a genetic disease resistance and shy. The paper focuses on microbial contamination of raw milk at 7 different studies of bull daughters in the same line for housing, feeding and conditions of use. Raw milk samples were obtained from the breed Latvian brown (LB) from dairy cows shed of cattle shed Saujas, which belongs to SIA Palsa and is located in the civil parish Varini, Smiltene county in the year 2008/2009. Compared between the lines of bulls' daughter milk yield of the sample average value of the uniformity of variance analysis shows that they significantly differ (F=2.452, p=0.039). Comparing each line of bulls' daughters SCC of the sample average value of the uniformity of variance analysis showed that the SCC was statistically significantly different (F=2.083, p=0.075) of the various lines of bulls daughters.

Genotypes of CSN3 gene were detected in 71 individuals of Latvian Blue breed and in 30 individuals of Latvian Brown breed using a PCR-RFLP method. Animals were chosen at random from each heard. This study investigated the alleles A and B of CSN3 gene, while determining the allele and genotype frequencies and Hardy-Weinberg equilibrium proportions in both populations. The results showed that in our analyzed samples from 71 Latvian Blue breed only 7 animals (10%) had the genotype BB, and in analyzed samples from 30 Latvian Brown breed only 3 had the genotype BB (10%). Frequencies of CSN3 genotypes AA, AB, and BB correspond to Hardy-Weinberg equilibrium proportions and populations in genetic equilibrium. A wide variation in the B allele frequency among Latvian Blue and Latvian Brown breeds was found suggesting that molecular selection for animals carrying the allele B of CSN3 could impact breeding programs for dairy production in Latvia.

The objective of the study was to investigate the microbiological content of cow (Bos primigenius) milk in Latvian organic farms according to season and herd size with a purpose to detect their impact on the distribution of mastitis causing pathogens in milk. Samples were collected in 14 organic dairy farms of Latvia, 4 times through 2012: in winter, autumn, spring and summer period. Raw milk samples (n=564) obtained from cow composite milk were studied. The samples were divided into three groups in accordance with the number of cows in the cow-shed: A (3-30), B (31-60) and C (61-124). The total colony count (TTC) and the isolation of mastitis causing bacteria were analysed using standard methods. Bacterial growth occurred in 90.4% of samples. Isolated microorganisms belonged to 35 species, and the following bacteria were the most prevalent agent, including Coagulase negative staphylococci in 29.4%, Staphylococcus aureus in 24.1%, Kocuria kristinae in 12.9%, and Enterobacteriaceae spp. in 10.3% out of 564 milk samples. Depending on the season, the average number of TCC was the lowest in summer (4.66 ± 4.01 log10 CFU mLE-1), moderately higher in spring and winter (4.72 ± 4.18 and 4.82 ± 3.54 log10 CFU mLE-1, respectively), but significantly higher in autumn (5.43 ± 4.80 log10 CFU mL-E1). Coliforms were not isolated from group A herds instead of B (3.1%) and C (4.6%). Occurrence of Staphylococcus aureus was noticeably higher in B (19.1%) and C (20.8%) than in A (12.6%) herds. Most of group A herds met the milk quality requirements, while group C herds produced more contaminated milk.

Advances in milk production and the expansion of dairy herds have increased the need for improved manure management and whole farm nutrient balance. It is well known in dairy management that the balanced feeding and holding technology is an important level by which milk production and milk composition can be modified. The objective of this study was to evaluate urea content and urea yield in different farms with different holding technologies. Four farms represent three cow breeds (Holstein Black and White, Latvian Brown, and cross breed XP). Individual cow milk samples (n=2740) were collected monthly from September 2009 to November 2010. Milk samples were analyzed for total protein, casein, fat, lactose, and urea content with instrumental infrared spectroscopy method. The average milk yield per cows in farms was significantly different (from 27.9 to 17.0 kg per control day). The urea content in cow milk was between 20.3 to 46.6 mg dLE-1. The average urea content in farms A, B and D was up to standard (from 15.0 to 30.0 mg dLE-1). In the farm C, average urea content ranged between 17.4 and 79.9 mg dLE-1, which indicated problems in feeding or management in the farm. It was established that milk urea content and yield significantly (p is less than 0.05) varied in farms with different dairy cow holding and feeding technologies. Urea content can be used to evaluate feed protein utilization in farms and predict environmental pollution.

The present study describes situation of antimicrobial resistance of animal pathogens and resistance trends in Estonia in years 2006-2009. Bacterial strains isolated during period 2006-2009 were Escherichia coli (E. coli), Enterococcus faecium (E. faecium), Enterococcus faecalis (E. faecalis), collected from healthy pigs faeces as well as from diagnostic submissions of pig samples. Staphylococcus aureus (S. aureus) isolates originated from cows with clinical mastitis and Staphylococcus pseudointermedius (S. pseudointermedius) isolates from dogs with pyoderma or otitis externa. Antimicrobial susceptibility was detected by microdilution method. Normal enteric microflora from health y pigs had resistance against streptomycin, tetracyclin, sulfametoxazol and trimethoprim. E. faecalis and E. faecium were resistant to erythromycin, tetracyclin, streptomycin and kanamycin. Multiresistance occured mainly against kanamycin, streptomycin and tetracyclin. E. coli strains isolated from pathological material showed high resistance to ampicillin, tetracycline, streptomycin, sulphonamides and trimethoprim. Multiresistance was detected between 60–73% during study years. In 2009, one ESBL (extended spectrum betalactamase) producing isolate was observed. S. aureus strains isolated from clinical mastitis samples were mainly penicillin resistant (58–86%). Meticillinresistant S. aureus was not found during the study. In 2009, resistance to lincomycin (30%) and fucidinic acid (22%) was detected. In S. pseudointermedius strains isolated from canine skin samples the prevalence of resistance to penicillin as high as 53–81% was found. Multidrug resistance was relatively stable being 38% in 2006, 29% in 2007 and 25% in 2009. In conclusion, antimicrobial resistance of animal pathogens in Estonia was high. Further improvement of prudent use of antimicrobials and infection control is needed.

The objective of the study was to examine how the circumstances of different bull daughters – Latvian Brown cow - group productivity levels correlate with the blood biochemical composition indices in Latvian conditions. The daughters of seven bulls, selected for our research trial, were kept in the same barn and under rearing conditions. Their blood samples were taken in a single sampling activity and analyzed for blood serum biochemical averages. Also Cu, Fe, Zn, Mn and Mg were detected in blood serum and the values found were compared with values fixed in regulatory documents. There were changes in Cu levels (daughter groups of four bulls), in Zn levels (one group); Mn level was lower in all groups and Mg level was higher in 6 groups. The altered biochemical parameters of blood were assessed from the pathogenetical point of view and compared with productivity averages in the progeny groups. The correlation factors suggested that there exist a strong positive correlation (p is less than 0.05) among a number of indicators, such as aspartate aminotransferase (ASAT) and protein r = 0.823; ASAT and somatic cell count (SCC), r = 0.737; blood protein and milk protein r = 0.903 - 0.917; albumins and protein r = 0.964; blood urea and milk protein r = 0.931 - 0.984 along with additional correlations. The correlation factors for the progeny group of each bull differed. The data obtained shows that the Latvian Brown cows crossed with other breeds produce cows with new genetic traits.

The objective of the present study was to investigate the microbiological content of cows’ milk in Latvia’s organic farms with a purpose to detect potential microbiological threats in milk. Samples were collected in December 2011 at 12 biological dairy farms of Latvia. Raw milk samples (N=155) obtained from cow composite milk were studied. The total mesophilic aerobic and facultative anaerobic microorganisms (MAFAM), the presence of coliforms and coagulase-positive staphylococci, count of yeasts and moulds were analysed using standard methods. Of the sampled cows 50% had a low somatic cell count (SCC) (LESS THAN 200,000 cells mLE-1), 23% - high, but 27% had a very high SCC (greater than 500,000 cells mLE-1). The mean value of MAFAM in the samples with low, high and very high SCC was 4.7, 5.0 and 5.0 log10 colony forming units (cfu) mLE-1, respectively. The yeasts were present in 57% of milk samples with the mean concentration of 3.1 log10 cfu mLE-1. Moulds were found in 27% of all milk samples; their mean concentration was 4.4 log10 cfu mLE-1. Identified mould strains belonged to genera Absidia, Aspergillus, Geotrichum, Mucor and Penicillium. In cases of subclinical mastitis and latent mammary infection the most distributed mastitis pathogens were Staphylococcus aureus, Micrococcus kristinae, Bacillus cereus and coagulase negative staphylococci.

The treatment of ketosis with glycerol was first introduced in the 1950s. Currently, the availability of glycerol as a feed supplement for dairy cattle has increased due to the rapid expansion of the biodiesel industry. It has been suggested that glycerol can be used as a dietary glucose precursor for dairy cows in a similar way to propylene glycol. This study examined the effect of orally administrated crude glycerol on milk production and composition, and energy-status related blood metabolites of primiparous (n=4) and multiparous (n=4) Holstein dairy cows in the first three weeks of lactation. The treatment group cows were given an oral drench of 500 mL of crude glycerol once a day before the morning feeding. Long-term oral drenching of crude glycerol had no effect on dry matter intake (DMI), but there was a positive effect on milk yield. Most milk composition values were not affected by the treatment, but treatment groups had lower milk protein levels. Blood glucose and insulin concentrations were declined with glycerol administration.

Milk production and milk composition are of prime economic importance for farmers. It is well known in dairy management that the balanced feeding and holding technology is an important lever by which milk production and milk composition can be modified. The objectives of this work are to establish relation among milk protein and urea content in different farms. Four farms represent three cow breeds (Holstein Black and White, Latvian Brown, and cross breed XP). Individual cow milk samples (n=8400) were collected monthly from September 2009 to November 2010. Milk samples were analyzed for total protein, casein, fat, lactose, and urea content with instrumental infrared spectroscopy method. The average milk yield in farms was significantly different (from 26.8 to 16.0 kg per control day), and average protein content varied from 3.32 g kgE-1 to 3.62 g kgE-1. The urea content in cow milk was between 21.3 to 42.6 mg 100 mLE-1. The average protein content was higher and significantly (p is less than 0.05) different in first yield level (up to 15 kg) for all farms. Overall, in different farm and milk yield levels correlation between protein and urea was low or very low. In the farm C, average urea content ranged between 30.0 and 60.0 mg 100 mLE-1, which indicated problems in feeding or management in the farm. It was established that milk productivity traits significantly (p is less than 0.05) varied in farms with different dairy cow holding and feeding technologies and milk protein and urea content significantly (p is less than 0.05) varied for cows with different milk yield per day.

The quality and nutritional value of milk and dairy products are considerably influenced by the stability of its constituents. The aim of the present study was to evaluate the possibility of enhancing oxidative stability of cow’s milk fat and vitamin B2 using carrots and palm oil feed supplement CAF 100 as sources of natural antioxidants in cow feed. Milk samples were collected after 25-day period of feed supplementation. The intensity of riboflavin losses during its photo oxidative degradation in sunlight was measured by the fluorometric method. A slight and significant (p is less than 0.05) difference in higher vitamin B2 stability was seen in carrot-supplemented group (TG1) milk, where the losses of vitamin B2 were by 3.01% less compared to CG (CG). The oxidative stability of butter oil samples stored in 60 °C temperature was analyzed by peroxide value (PV) method. The oxidative stability of samples initially affected by light from both trial groups was significantly (p is less than 0.05) higher compared to CG showing the good potential of the cow diet enrichment with carotenoid additives. The longest induction period (larger than 14 days) was observed in CAF-100- supplemented (TG2) cow milk fats, which can be explained by carotenoids and tocopherol presence and its possible synergism in fat protection. The induction periods of the TG1 and CG were 12.03 and 10.97 days, respectively.