Genotypes of CSN3 gene were detected in 71 individuals of Latvian Blue breed and in 30 individuals of Latvian Brown breed using a PCR-RFLP method. Animals were chosen at random from each heard. This study investigated the alleles A and B of CSN3 gene, while determining the allele and genotype frequencies and Hardy-Weinberg equilibrium proportions in both populations. The results showed that in our analyzed samples from 71 Latvian Blue breed only 7 animals (10%) had the genotype BB, and in analyzed samples from 30 Latvian Brown breed only 3 had the genotype BB (10%). Frequencies of CSN3 genotypes AA, AB, and BB correspond to Hardy-Weinberg equilibrium proportions and populations in genetic equilibrium. A wide variation in the B allele frequency among Latvian Blue and Latvian Brown breeds was found suggesting that molecular selection for animals carrying the allele B of CSN3 could impact breeding programs for dairy production in Latvia.

The aim of the research was to evaluate the variability of milk yield and composition for goats with different kid suckling periods. The research was carried out in 2011 and 2012 on a farm where goats of Latvian breed were reared. In both years goats kidded in February and March. Each group included 10 animals. In the first group the kids were weaned on the first day of kidding (A1), in the second – on the 30th day (A30) but in the third – on the 60th day (A60) after kidding. The amount of the milk yield was measured in five successive days after the kid weaning. Milk samples were analysed in an accredited milk laboratory. The highest milk yield was obtained from the goats when kids were weaned immediately after the birth, and samples were taken starting with the 6th day of lactation (2.10 + 0.05 kg), but the lowest from the goats with suckling period of 60 days – 1.68 ± 0.03 kg. The average milk fat and protein content of the goats from the group A1 (53.6 ± 0.92 and 41.6 ± 0.66 g kgE-1), was significantly higher than from the groups A30 and A60 (p is less than 0.05). The average variability of milk yield proved to be from 4.9% in the group A1 to 10.5% in the group A30. The lowest fat content variability was observed for goats of the group A1 in both years – 8.9% and 10.7%, but the highest 20.5% in the group A60 in the first year. The highest variability of milk protein content was observed in the group A30 – 14.8%.

The objective of the study was to examine how the circumstances of different bull daughters – Latvian Brown cow - group productivity levels correlate with the blood biochemical composition indices in Latvian conditions. The daughters of seven bulls, selected for our research trial, were kept in the same barn and under rearing conditions. Their blood samples were taken in a single sampling activity and analyzed for blood serum biochemical averages. Also Cu, Fe, Zn, Mn and Mg were detected in blood serum and the values found were compared with values fixed in regulatory documents. There were changes in Cu levels (daughter groups of four bulls), in Zn levels (one group); Mn level was lower in all groups and Mg level was higher in 6 groups. The altered biochemical parameters of blood were assessed from the pathogenetical point of view and compared with productivity averages in the progeny groups. The correlation factors suggested that there exist a strong positive correlation (p is less than 0.05) among a number of indicators, such as aspartate aminotransferase (ASAT) and protein r = 0.823; ASAT and somatic cell count (SCC), r = 0.737; blood protein and milk protein r = 0.903 - 0.917; albumins and protein r = 0.964; blood urea and milk protein r = 0.931 - 0.984 along with additional correlations. The correlation factors for the progeny group of each bull differed. The data obtained shows that the Latvian Brown cows crossed with other breeds produce cows with new genetic traits.

The aim of the present research was to evaluate the affect of genetic and environmental factors on the variability of the goat milk yield, fat and protein content in goat milk in Latvia. Data of 6067 lactation records from 2400 goats of six different breeds were analysed in the period of 2001 to 2010. The highest milk yield (662.7±14.58 kg) was obtained in 2002, the highest fat content (40.7±0.05 g kgE-1) – in 2004, but highest protein content (32.7±0.19 g kgE-1) – in 2008 (p is less than 0.05). Basically there are two goat breeds in Latvia: Latvian goats and Saanen goats. In the research, 3261 Latvian and 2032 Saanen goats in closed lactations were analysed. It was found that Saanen goats gave the highest milk yield (579.3±5.01 kg), but Alpine goats – the highest fat and protein content (respectively 41.7±0.63 and 32.3±0.30 g kgE-1; p is less than 0.05). It was observed that most of all the goats kidded in winter (2379) and spring (3378). The highest milk yield (583.8±7.39 kg) was determined for goats kidded in winter season, but the highest fat content (41.8±0.06) and protein content (32.3±0.03) – for goats kidded in summer season. The average milk yield in the first lactation (1636) was significantly lower than in the third lactation (578.0±8.34; p is less than 0.05) when the goats produced the highest milk yield in the research.

Milk production and milk composition are of prime economic importance for farmers. It is well known in dairy management that the balanced feeding and holding technology is an important lever by which milk production and milk composition can be modified. The objectives of this work are to establish relation among milk protein and urea content in different farms. Four farms represent three cow breeds (Holstein Black and White, Latvian Brown, and cross breed XP). Individual cow milk samples (n=8400) were collected monthly from September 2009 to November 2010. Milk samples were analyzed for total protein, casein, fat, lactose, and urea content with instrumental infrared spectroscopy method. The average milk yield in farms was significantly different (from 26.8 to 16.0 kg per control day), and average protein content varied from 3.32 g kgE-1 to 3.62 g kgE-1. The urea content in cow milk was between 21.3 to 42.6 mg 100 mLE-1. The average protein content was higher and significantly (p is less than 0.05) different in first yield level (up to 15 kg) for all farms. Overall, in different farm and milk yield levels correlation between protein and urea was low or very low. In the farm C, average urea content ranged between 30.0 and 60.0 mg 100 mLE-1, which indicated problems in feeding or management in the farm. It was established that milk productivity traits significantly (p is less than 0.05) varied in farms with different dairy cow holding and feeding technologies and milk protein and urea content significantly (p is less than 0.05) varied for cows with different milk yield per day.

Live weight and body condition are indicators for dairy cow’s (Bos taurus) health, milk productivity and reproduction. Live weight and body condition are defined by genetic and non-genetic factors. These factors are dependent on dairy cows growing and welfare. The aim of research was to analyze body condition relationship with milk productivity and live weight. Data were collected from 49 different breed and lactation dairy cows. Research location was Latvia University of Agriculture Research and Study farm ‘Vecauce’. Data were collected from October 2013 to January 2014. Body condition score of all cows decreased from 2.8 ± 0.05 to 2.5 ± 0.04 points in research period. Milk yield increased from 35.6 ± 0.79 kg in the 1st recording to 40.9 ± 1.12 kg in the 2nd recording. Milk yield decreased in the 3rd recording (p is less than 0.05). Fat content was the lowest in the 2nd recording (35.5 ± 0.09 g kgE-1). Protein content was significantly different in the 1st and 2nd recordings (p is less than 0.05). Somatic cell changes were not significant. Body condition decreased of older lactation cows, but milk yield increased at the same time. Milk yield was significantly the greatest in red breed group, compared with Holstein black and white cows (51.1 ± 3.21 kg vs. 41.4 ± 0.78 kg; p is less than 0.05). Body condition score significantly affected live weight in such body condition score groups: less than 2.5 points, 2.75 to 3.0 points. Effect was not significant on live weight in body condition score 3.25 less than group. Milk productivity and quality traits were not affected by the body condition score (p is less than 0.05).

The aim of our study was to determine up to what extent the number of services per calving (NSPC) affects dairy cow productive and reproductive performance. The study contains data from the Agricultural Data Centre of Latvia about 26888 Latvian Brown (LB) and Holstein Black and White (HBW) breed cows that were born from year 2005 – 2010, closed at least 5 full lactations and were culled from herds. In data set we have included data about cow milk productivity (calculated in ECM), longevity traits and reproduction traits. The reproduction traits included the number of services per conception (NSPC), calving interval (CI) and calving to conception interval (CCI) in the first five lactations. The average lifespan of LB and HBW dairy cows was 3149.5 days and in this period 39570.6 kg ECM were obtained. LB breed cows were characterized by 23.5 days longer lifespan and by 6035.4 kg ECM lower lifetime milk productivity than HBW breed cows, but they showed higher reproduction trait scores than LB breed group. HBW cows had a significantly (p is less than 0.05) higher NSPC, CCI, number of milking days (MD) and CI than LB breed cows in all five analysed lactations. The NSPC in the first lactation increased the calving age at the fifth lactation – cows with NSPC in the first lactation is greater than 4, fifth time calved 170.7 days in LB breed group and 190.8 days in HBW breed group later than cows with one NSPC in the first lactation.

This article presents the results of a comparative evaluation of preventive treatment of udder with probiotic agents: ‘Dipal’ (manufacturer DeLaval - Sweden), ‘Zorka’ (manufacturer «NPP Farmaks» - Russia). We have received data on the effects of probiotic preparation on quality of milk and number of somatic cells in milk. Research has been conducted in two dairy farms of Almaty region of the Republic of Kazakhstan. Lactobacillus acidophilus 05ch - isolated from shubat (South Kazakhstan region, Turkestan). Lactococcus lactis 010k - isolated from three-day kumys (Zhambyl region, Merke). These probiotics are used as a means for sanitary treatment of the udder of cows. Thus, the procedure of determining the antagonistic activity revealed their high activity against both gram-negative and gram-positive microorganisms, notably Staphylococcus aureus (10-13 mm), Escherichia coli (12 mm), Proteus vulgaris (10-14 mm), Salmonella abortus ovis (11-13 mm). Significant changes in the milk indices can be found in the experimental group: 9.1% rise of lactose, and 7.2% reduction of protein. The level of protein increased by 4.1% , whereas the amount of lactose, on the contrary, went down 1.6%, which may be indicative of the increase in the content of serum protein when the udder is inflamed. The examination of the milk obtained from the cows of the control and experimental groups showed a nearly double reduction in the number of somatic cells from 488.00 down to 178.000 thousand, which was caused by the daily treatment of cows by probiotic cultures during 2 weeks.

The purpose of this study to compare milk calcium and phosphorus content of different breeds raised in Estonia. Based on obtained results, different factors and their magnitude of influence on the contents of calcium and phosphorus in milk were studied. On Polula Research Farm five trial groups were made up: Estonian Holstein breed (breeding value 115) (EHF-t), Estonian Holstein breed (breeding value 105...115) (EHF), Red-and-White Holstein breed (RHF), Estonian Red breed (EPK) and Estonian Native breed (EK). Milk samples were collected from five of each trial group twice a month during the year 2001. Calcium and phosphorus contents were determined by using IDF methods 36A:1992 and 42B:1990 in Dairy Laboratory of the Institute of Animal Science.

The goal of this study was to investigate the use of body reserves, energy balance and fertility parameters at the beginning of lactation in first-parity cows bred in Estonia.