Nine multiparous cows were biopsied twice – in the first and fifth week after parturition in winter 2004/2005 on the Research and Study farm Vecauce of Latvia University of Agriculture. The aim of the research was to reveal distribution of inflammatory factors and apoptosis in the cow’s endometrium in post parturition period. Haematoxylin and eosin were used for routine staining of each sample. Tumor necrosis factor α (TNFα, w.d. 1:100, Abcam, England), and interleukin 10 (IL10, w.d. 1:400, Abcam, England) were detected immunohistochemically (IMH), and TUNEL method was used for detection of apoptosis. Distribution of these factors and substances was determined semiquantitatively by counting positive structures in visual fields. A significant increase in the total amount of inflammatory cells, as well as in the amount of the TNFα and apoptotic cells in endometrium between the first and fifth week after parturition (p is less than 0.01) was observed. A significant positive correlation (r=0.41; p is less than 0.01) between infiltration of endometrium with neutrophils and with macrophages was detected in the first week after parturition. A mild positive correlation (r=0.52; p is less than 0.01) between neutrophils and lymphocytes, as well as between lymphocytes and macrophages (r=0.35; p is less than 0.01) was found four weeks after parturition. A mild positive correlation (p is less than 0.05) was observed between amount of inflammatory cells and TNFα (r=0.52), and number of apoptotic cells and TNFα (r=0.58). The research suggests that inflammatory factors and apoptosis intensively act during the process of involution in the cows’ endometrium from the first up to the fifth week after parturition. Intensity, amount and activity of these components show individual variations in each case.

The aim of the study was to find out the differences of blood biochemical and morphological parameters in cows with and without retained foetal membranes. Sixty cows of different age of Latvian blackand- white breed from two Joint Stock Companies were used in the research that was divided into two groups: control group of 15 cows, and 45 cows with retained foetal membranes. Research was done from September 2007 to January 2009. In haematological parameters on the second day postpartum, control group cows had increased leukocyte count on the account of mature neutrophils, but in the group of cows with retained foetal membranes leukocytes were within the range of normal values. The difference of the above mentioned parameters between both of the groups was significant (p is less than 0.05). At the same time, in cows of both investigated groups the number of band neutrophils was slightly increased (p is greater than 0.05). Haematological analyses showed leukocytes shift to the left and lymphopenia with a tendency to get normal within six weeks postpartum in both cow groups. On the third week postpartum, in cows with retained foetal membranes the band neutrophil count increased significantly (p is less than 0.05) indicating that the inflammation process has flared up. Blood for biochemical examination was sampled from cows within 48 hours postpartum in order to evaluate the functional condition of liver in milking cows. A significantly higher (p is less than 0.05) total and direct reacting bilirubin was observed in cows with retained foetal membranes, although it was within the range of normal values.

There are several unique features in the anatomy of the stomach in African ostrich in comparison with other birds. The goal of this study was to determine changes in the main morphometric parameters and histological features of gastric mucosa in ostrich chickens from the 38th day of embryonal development until 60 days of age. For the study, 6 embryos (38th day of development) and 36 chicks (1, 3, 7, 14, 30, and 60 days post hatching; 6 chicks per time point) of both sexes were obtained from African ostrich farm in Latvia during May - October, 2009. Tissue samples were investigated in Preclinical Department, Faculty of Veterinary Medicine. The total area of proventricular mucosa (cm2) and surface area of deep glands (cm2) was measured. Tissue sections for histological assessment were stained with: hematoxylin and eosin and alcian-blue pH 2.5 - periodic acid – Schiff reaction. Thickness of proventricular and ventricular mucosa, depth of proventricular superficial glands as well as surface area of parenchyma occupied by deep glands were measured in histologic sections. As the chick age increased, there were changes in the ratio of areas occupied by proventricular superficial and deep glands with relative decrease in area occupied by deep glands. Proventricular deep glands developed rapidly after hatching; at the age of 30 days deep glands histologically resembled glandular structure of adult birds. Gastric mucosal epithelial cells of 38 days old ostrich embryos and of just hatched ostrich chicks contained neutral, acidic, and mixed mucopolysaccharides. Meanwhile, in the pyloric region of ventriculus acidic mucopolysaccharides predominated.

The treatment of ketosis with glycerol was first introduced in the 1950s. Currently, the availability of glycerol as a feed supplement for dairy cattle has increased due to the rapid expansion of the biodiesel industry. It has been suggested that glycerol can be used as a dietary glucose precursor for dairy cows in a similar way to propylene glycol. This study examined the effect of orally administrated crude glycerol on milk production and composition, and energy-status related blood metabolites of primiparous (n=4) and multiparous (n=4) Holstein dairy cows in the first three weeks of lactation. The treatment group cows were given an oral drench of 500 mL of crude glycerol once a day before the morning feeding. Long-term oral drenching of crude glycerol had no effect on dry matter intake (DMI), but there was a positive effect on milk yield. Most milk composition values were not affected by the treatment, but treatment groups had lower milk protein levels. Blood glucose and insulin concentrations were declined with glycerol administration.

Stomach, as a part of the digestive canal, is important in the metabolic processes of the organism which affects the bird’s general health condition, its growth and development, consequently also the increase of the body weight. The aim of the research was to find out the dynamics of weight, length, and area of the ostrich (Struthio camelus) stomach and its parts in postnatal ontogenesis from 4 to 12 months of age. The absolute weight of stomach, length of the greater curvature of the stomach glandular part, diameter of the muscular part, thickness of stomach wall was determined. To find out the differences of the mean values between various age groups, ANOVA and Post Hoch tests were used. The increase of the stomach absolute weight continued throughout the postnatal ontogenesis period, but the stomach weight in relation to the ostrich body weight decreased, especially rapidly at the age of 6 and 8 months. At the same time, proportions of the absolute and relative weight of the stomach glandular and muscular part changed. The length of the greater curvature of the stomach glandular part, length of the deep gland region and diameter of the stomach muscular part increased during the whole observed period of ontogenesis; moreover, these parameters increased more rapidly from 6 to 8 months of age. The area of the stomach glandular and muscular part mucosa increased throughout the observed period of ontogenesis. The area of the superficial gland region in all age groups was larger than the area of the deep gland region.