The aim was to study changes of chemical composition (fat content, protein content), pH and colour of poultry meat during chilled storage. Various criteria are used for evaluation of bird and carcass quality, while the combination of several indices determines the quality of poultry meat, which has an important role in the following meat processing and quality of end product. Chilled poultry meat is offered in retail and it is used in processing plants for various meat products production. Chemical composition (fat content, protein content), pH and colour are important quality determinants. Hens' meat is cheaper compared to broiler meat; therefore its use in processing is profitable. Parents stock hens of the cross Ross 308 were used for the study. Parent stock hens were fed compound feed. After chilling a hen fillet [musculus pectoralis], hen thighs [musculus biceps] and hen drumsticks [musculus gastrocnemius; musculus fibularis longus] were separated from carcasses. The obtained products were refrigerated at temperature +1+-0.5 deg C. The analyses were performed 1 day after slaughter and on the 5th day of storage. The following parameters were analysed: pH value (3510pH Meter - JENWAY); total protein content (LVS ISO 937:1978); fat content (LVS ISO 1443:1973); colour changes (Color Tec-PCM; software ColorSoft QCW for colour data analysis). The analysed parameters significantly differ in 'white' and 'red' poultry meat, and in various cuts of 'red' meat.

The non-spore forming gram-positive bacterium Listeria monocytogenes is a food pathogen bacterium and the causative agent of listeriosis. The aim of study was to determine the survival limits of L. monocytogenes inoculated in manufactured cold smoked sausages depending on water activity (aw) and pH values. Enumeration of L. monocytogenes colony forming units per gram (cfu gE-1) was done according to ISO standards. The decreasing water activity conditioned by moisture (weight) loss during ripening and pH decrease ensured negative exponential growth rate of inoculated L. monocytogenes lg cfu gE-1 - 0.44 each day. A significant Pearson's correlation (p is less than 0.01) was established between decreased values of L. monocytogenes count, aw (0.99), pH (0.92), moisture % (0.96), and weight loss (0.93) in sausages during ripening. The experiments were done at the Faculty of Veterinary medicine of the Latvia University of Agriculture and in a sausage manufacturer's laboratory.

The major sugars in six cultivars grown in Latvia are analysed. The content of glucose, fructose and sucrose in strawberry, blackcurrant and redcurrant berries was determined by applying the method of high performance liquid chromatography (HPLC). By using the obtained calibration curves, concentrations of diverse sugars were found in the analyzed samples. Fructose or fruit-sugar, the same as glucose, is mainly the source of energy in the human body though it is decomposed faster and easier than glucose. In all the researched berry cultivars, the amount of fructose was the largest, particularly in blackcurrants. Six strawberry, black- and redcurrant cultivars were evaluated as raw materials for industrial production. The investigation involved chemical and sensory analyses of fresh berries. The results indicate that pH and sugars of fresh berries influence their quality. Furthermore, high level of sugar adversely affects taste stability. Taste degradation in berries is parallel to flavour deterioration. It is concluded that the main emphasis should be given to the properties of fresh berries.

Venison is well known as a traditional meat type in Europe, and it is lower in calories, cholesterol and fat content than common cuts of beef, pork or lamb. The aim of the current research was to determine physico-chemical parameters such protein, fat, pH, moisture content as well microbiological quality of pickled venison during storage. Beef as a control was analyzed for comparison of obtained results. The meat (2×3×2 cm) pieces were pickled in vinegar marinade (composition: tomato sauce, mayonnaise, vinegar, lemon, onion, parsley, paprika, basil, black pepper, rosemary, salt) at 4±2 °C temperature for 48±1 h. The marinated meat was placed in polypropylene trays and hermetically sealed with high barrier polymer film under modified atmosphere (C02 40%+N2 60%) without and with iron-based oxygen scavenger sachets (Mitsubishi Gas Chemical Europe Ageless®). As a control, packaging in air ambiance packed pickled products was used. During storage time, the moisture and protein values significantly (p is less than 0.05) decreased and pH, fat content and colony forming units significantly (p is less than 0.05) increased in the pickled venison samples of all packages. Slower changes in pH of pickled venison and in the protein and moisture content of marinated beef were observed in modified atmosphere with oxygen scavenger during storage.

The survival of inoculated in a cold-smoked sausages Listeria monocytogenes wild strains was studied. The sausages were prepared with and without starter cultures. The survival limits of L. monocytogenes and lactic acid bacteria (LAB) were determined as colony forming units per gram (cfu gE-1) depending on water activity (aw) and pH on 0, 1st, 3rd, 5th, 7th, 14th and 21st days of maturation. The decreasing water activity conditioned by moisture (weight) loss during ripening and pH decrease ensured negative polynomial growth rate of inoculated L. monocytogenes - 0.27 lg (cfu gE-1) each day of ripening time, and - 0.65 lg (cfu gE-1) on the first 7 days of maturation. A significant Pearson’s correlation (p is less than 0.01) was established between decreased values of L. monocytogenes count, aw, salt concentration and LAB growth in sausages during the ripening period of 21 days. The main parameters, maintained negative exponential growth rate of L. monocytogenes in cold smoked sausages, are aw value decrease and LAB (starter culture), which stopped L. monocytogenes growth at the beginning of cold-smoked sausage maturation. If fermentation process went technically and hygienically correctly, the fermented (cold-smoked) sausages could be one of the safest meat products, because in real practice a low level contamination has been seen. The remaining count of L. monocytogenes in cold-smoked sausage depends on the possible initial contamination level and could exceed the European Union regulation value 2.0 lg (cfu gE-1) for ready-to-eat products when contamination at first is more than lg 5.0.

The non-spore forming gram-positive bacterium Listeria monocytogenes is a food pathogen bacterium and a causative agent of listeriosis. The aim of the study was to determine the survival limits of L. monocytogenes inoculated in manufactured vacuum-packed cold smoked pork depending on shelf time, supported by water activity (aw) and pH values. Enumeration of L. monocytogenes colony forming units per gram (cfu gE-1) was done according to ISO standard. Water activity (aw) and pH values in pork samples were more or less constant and supported L. monocytogenes growth. The behaviour of L. monocytogenes in cold-smoked sliced pork by shelf time, when environmental factors changed minimally and supported growth, largely depended on the initial contamination level. A lag-phase of bacterial growing process before exponential growth rate of inoculated L. monocytogenes depended on initial cell concentration and had 10 days step level if storage temperature was approximately 5 deg C. A significant Pearson’s correlation (p is less than 0.01) was established between the microbiological test values of L. monocytogenes count changes in sliced and packed cold-smoked pork during storage time of 60 days. The main parameter which maintained negative polynomial growth rate of L. monocytogenes in cold-smoked pork was the decrease of live cell concentration in samples below lg 2.0. The experiments were done at the Faculty of Veterinary Medicine of Latvia University of Agriculture and at a sausage manufacturer’s laboratory.

There is growing interest in products without added sugar; thus, sugar consumption is directly related to diabetes and other illnesses such as obesity. A change in the type of sugar and content may therefore both change the perception of sweetness and texture of products. The aim of the research work was to evaluate properties of agar-agar jellies prepared with inulin syrup, galactose syrup and malt extract to replace sugar. Agar gels were prepared to substitute sugar with inulin syrup, galactose syrup and malt extract. Texture of experimental samples was determined by using a Texture Analyser (Model TA.XT Plus; Stable Micro Systems). Colour of jellies was evaluated by using Colour Tec-PCM and Jenway 3510 was used for pH measurements. Obtained results showed that different kinds of sugar containing syrups could be used as sugar substitute for production of a new type jellies. Hardness of the experimental samples is influenced by the sugar type containing syrup. The hardness is determined in the experimental samples by replacement of sugar by increasing concentration of inulin syrup. Decreasing hardness values are observed by increase of added malt extract (maltose) and galactose syrup concentration in samples. The pH values of experimental samples ranged between 3.11 and 4.45. Higher L* value of experimental samples are with galactose syrup. Lightness “L” changed between 20.92 and 18.11 increasing inulin syrup concentration. The same situation can be observed using malt extract as sugar substitute.

Growing interest of consumers in healthy eating has provided the development of new range of food. Therefore the task of research was to determine the nutritional value, calculate energy value and investigate the sensory properties of yoghurt samples enriched with flakes from biologically activated hull-less barley grains and malt extract. Results showed that by adding flakes from biologically activated hull-less barley grain and malt extract it was possible to improve the nutritional value of yoghurt, i.e., increased protein, carbohydrate and decreased fat content. The energy value of yoghurt samples enriched with flakes from biologically activated hull-less barley grain and malt extract ranged between 65.96 and 75.72 kcal 100 gE-1, which is significantly lower comparing with mean energy value of commercial yoghurts. The changes of sensory properties were affected by the amount of added malt extract in yoghurt samples. The optimal amount of added malt extract for sensory evaluation in yoghurt samples was determined as 2%. The content of carbohydrate in yoghurt sample enriched with 5% of biologically activated hull-less barley grain and 2% of malt extract was two times lower than commercial yoghurts therefore its energy value was significantly lower. Yoghurt enriched with flakes from biologically activated hull-less barley grain and malt extract could be competitive.

Cereal and whole grain products are an important source of dietary fiber in the human diet. Since the milling process highly influences the proportions of the different cell types in the bran, it is expected that brans originating from different genetic/agricultural backgrounds and produced by different processes have different chemical composition. Wheat (Triticum aestivum) bran has not been studied in Latvia, therefore the aim of the present study was to analyze physical – chemical characteristics of wheat bran from Latvia’s industrial mills. Four bran samples were collected from two industrial mills: Stock Company ‘Dobeles dzirnavnieks’ and SC ‘Rigas dzirnavnieks’. All experiments were performed at the Food Technology of Latvia University of Agriculture in May 2011-February 2012. The particle size distribution of the bran samples was determined by sieving. The content of titratable acidity (TA) was detected by titration with 0.1 M NaOH till pH 8.5 was stable for 1 minute. Wheat bran colour was analysed by Hunter Lab colour measurement instrument using ColorTec colorimeter PCM/PSM in CIE L * a * b* system. Moisture content was analyzed using standard ICC 110/1 method by sample drying for 2 h at 150 °C. Results showed that there were significant differences between varieties (p0.05) for titratable acidity and for particle size, but no significant differences (p0.05) were found between the varieties in moisture, pH and colour. TA varied from 6.40±0.71 to 12.05±0.21°, moisture content- from 10.01±0.51% to 11.61±0.47%, pH–from 6.31±0.61 to 6.80±0.05, but colour of bran between the samples did not significantly differ.

The present study was undertaken to estimate enzymatically hydrolysed and non–hydrolysed wheat (Triticum aestivum) and rye (Secale cereale) bran microflora. Enzymatic hydrolysis was accomplished by α – amylase from Bacillus amyloliquefaciens and by Viscozyme L which contain a wide range of enzymes responsible for the breakdown of carbohydrates into simple sugars. Wheat and rye bran samples were collected from native mills, namely Stock Company (SC) ‘Rigas dzirnavnieks’ wheat bran with large particle size (WLSR), SC ‘Jelgavas dzirnavas’ rye bran with small particle size (RSSJ), SC ‘Dobeles dzirnavnieks’ wheat bran with small particle size (WSSD) and wheat bran with large particle size (WLSD). Gained results indicate that before enzymatic hydrolysis all of the bran samples showed similar microbiological contamination with total plate count (TPC), yeasts and lactic acid bacteria. Enzymatic hydrolysis of bran gives the possibility to partially eliminate the microbiological contamination with TPC, yeasts and lactic acid bacteria. The amount of microorganisms after enzymatic hydrolysis (before storage) were decreased and ranged from 5.26 ± 0.04 to 5.45 ± 0.01 log CFU gE-1, from 4.81 ± 0.01 to 5.60 ± 0.05 log CFU gE-1, and from 4.09 ± 0.01 to 5.10 ± 0.05 log CFU gE-1, respectively. After eight weeks of storage (temperature – 20 ± 1 °C, relative humidity – 40 ± 1%) enumeration of microorganisms showed significant decrease of colony–forming units in all bran samples. The amount of TPC, yeasts and lactic acid bacteria in the control bran samples fluctuated in a range from 4.84 ± 0.04 to 5.49 ± 0.05 log CFU gE-1, from 4.86 ± 0.03 to 5.25 ± 0.03 log CFU g-1, 3.53 ± 0.03 to 4.21 ± 0.02 log CFU gE-1 respectively.