The traditional way of dealing with manure - storage, transportation, spreading in the fields - is currently applied very successfully, but is economically very expensive. People complain about very unpleasant smells, which are caused by gasses (indole, merkaptanas, hydrogen sulphide, ammonia) being released from the manure. Scientists are searching for other ways of handling manure which try to convert it to less harmful substances, reduce its odour and at the same time make it thicker by extracting its water content. The aim of our work was to evaluate the composition of slurry after processing it mechanically, and to test the effectiveness of the application of new coagulants and flocculants on its clarification. Fresh liquid manure from 'Sajas' pig complex(Kelme district) and the liquid manure treated in biogas reactors from Lekėčiai pig complex (Šakiai district) was used for research (2006-2008). Anionic coagulants such as aluminium chloride (Al2Cl), ferrous chloride (Fe2Cl) and pollycrilamide cationic flocculant 'Magnofloc 1011' were applied to clarify manure. 40-44% of organic pollutants can be removed from the aerated sewage under constant processing by coagulants and flocculants. The best result in pollutant removal from sewage is achieved when it is processed with 1.5 kg mE-3 norm of aluminium saline (AlCl) and 3.0 g mE-3 norm of flocculant 'Magnofloc 1011'.

The effect of dietary supplement with 17% high-amylose barley (Hordeum vulgare, HAB) 'Karmose' on boar taint was studied on 72 crossbred entire male pigs (Sus scrofa domestica) Swedish Yorkshire dams x Swedish Landrace sires). All pigs were fed the same commercial diet (12.4 MJ metabolised energy (ME) per kg, digestible crude protein (CP) 13.5%) twice a day until the average live weight (LW) in the pen reached 100 +- 11.8 kg. Then, 32 out of 72 pigs received an HAB diet. Half of these pigs received HAB for 14 days and the other half for 28 days. The pigs were slaughtered at an average LW of 122.3 +- 8.6 kg (age 167.2 +- 7.5 days). The HAB diet did not affect performance, carcass and meat quality characteristics, and puberty status, but reduced plasma skatole levels in the pigs (least squares means, 3.6 vs. 2.0 ng mlE-1, P = 0.037). However, this diet failed to reduce skatole, indole and androstenone levels in fat (P is greater than 0.10). Nevertheless, it is desirable to continue investigation of HAB to optimise the feeding procedure to improve efficiency of HAB effect on fat skatole levels. Additionally, validation of a greater amount of HAB in the diet should be performed.

Technologies of keeping livestock and management of manure pig complexes holdings built in the country under the Soviet regime no longer satisfy modern veterinary and environmental requirements. Conditions for the modernisation of such complexes were created as from 2000, when agricultural aid programmes (funds) appeared in Lithuania. Information about old and newly implemented manure management technologies, fertilisation value of manure, and quality of the drainage runoff from manure-fertilised fields has been collected in six large livestock companies. Analysis of collected data revealed that new livestock keeping, feeding and manure management technologies are more cost-effective in using energy and mineral resources and conform to the animal welfare, environmental protection and hygiene requirements that are becoming more and more stringent. As the traditional manure management in economic terms is quite expensive and has a significant impact on the environmental quality, academic community is looking for new manure processing methods. The global development of manure management technologies focuses on the manure processing in biogas plants. Biogas generated from liquid manure and animal waste in such plants is an alternative source of energy.

The data on chemical composition of groundwater in the boreholes installed in the fields of a swine-breeding complex JSC Kontvainiai irrigated by liquid manure and in the production territory are presented in the article. This complex is situated on the Lithuanian coast, southeast of Klaipeda district. The data covers the period 2001-2005. It was established that the chemical composition of groundwater depends mainly on the soil. Ion sum was 2 times higher in the water of the well which was installed in the soil having a peat layer. The chemical composition of groundwater depended as well on its movement direction. As groundwater moves to the southeast, south and southwest from the fertilized area, the mineralization of water in wells 1 and 4 was the highest – 699 and 728 mg lE-1, respectively.

The present study describes situation of antimicrobial resistance of animal pathogens and resistance trends in Estonia in years 2006-2009. Bacterial strains isolated during period 2006-2009 were Escherichia coli (E. coli), Enterococcus faecium (E. faecium), Enterococcus faecalis (E. faecalis), collected from healthy pigs faeces as well as from diagnostic submissions of pig samples. Staphylococcus aureus (S. aureus) isolates originated from cows with clinical mastitis and Staphylococcus pseudointermedius (S. pseudointermedius) isolates from dogs with pyoderma or otitis externa. Antimicrobial susceptibility was detected by microdilution method. Normal enteric microflora from health y pigs had resistance against streptomycin, tetracyclin, sulfametoxazol and trimethoprim. E. faecalis and E. faecium were resistant to erythromycin, tetracyclin, streptomycin and kanamycin. Multiresistance occured mainly against kanamycin, streptomycin and tetracyclin. E. coli strains isolated from pathological material showed high resistance to ampicillin, tetracycline, streptomycin, sulphonamides and trimethoprim. Multiresistance was detected between 60–73% during study years. In 2009, one ESBL (extended spectrum betalactamase) producing isolate was observed. S. aureus strains isolated from clinical mastitis samples were mainly penicillin resistant (58–86%). Meticillinresistant S. aureus was not found during the study. In 2009, resistance to lincomycin (30%) and fucidinic acid (22%) was detected. In S. pseudointermedius strains isolated from canine skin samples the prevalence of resistance to penicillin as high as 53–81% was found. Multidrug resistance was relatively stable being 38% in 2006, 29% in 2007 and 25% in 2009. In conclusion, antimicrobial resistance of animal pathogens in Estonia was high. Further improvement of prudent use of antimicrobials and infection control is needed.

Antibiotic resistance of Staphylococcus is increasing worldwide. New antibiotics are used in big amounts in the meat production more and more. As a zoonosis, Staphylococcus aureus (S. aureus) is found in various species of animals and people, especially in those, who are working on commercial swine farms and slaughterhouses. The aim of the study was to find out the occurrence of S. aureus in different age groups of pigs on commercial swine farms in Latvia. Microbiological samples (n=702) including nasal, rectal, milk and air samples were collected during October 2013 from three closed farms of different sizes and several age groups of pigs and investigated with microbiological standard methods. S. aureus was found in all swine farms. The occurrence of S. aureus in Latvian pig farms was 41% and the highest occurrence of S. aureus was among 3-3.5 month old piglets. S. aureus was 1.8 times more frequently found in nasal than in rectal samples, and only in 28.38% of pigs S. aureus was in both - nasal and rectal samples. S. aureus was found also in sow milk in 13% of samples and in 3 samples of air (n=23).

The aim of study was to investigate porcine circovirus-2 (PCV2) impact on the morphological sight of pig lymph nodes. The research was carried out in 2010. Three Latvian farms were investigated (from Latgale and Kurzeme) and thirty 5-15 weeks old pigs with clinical signs of Postweanig Multisystemic Wasting Syndrome were selected and observed. Four lymph nodes from each animal for histological and immunohistochemical investigations were taken. Thirty structural parameters of a lymph node were detected on histological slides: visualization of follicle structure, lymphocyte depletion and amount of histiocytic cell infiltration. As a result, changes in lymph nodes with PCV2 antigen were detected in all these structural parameters: increasing amount of PCV2 antigen in pig lymph nodes, but the greatest changes were in the follicles structure in lymph node. The increased amount of PCV2 antigen in lymph node; significantly decreases the amount of lymphocytes and increases the amount of histiocytes in them. Porcine circoviruss-2 in lymph node basically distributes from the lymph node cortex into the medulla, as well the amount of PCV2 antigen in lymph node also increased.

The aim of the present study was to investigate the histological status of lymph nodes lnn. inguinales superficiales sinister et dexter, lnn. mesenterici cranial, and lnn. tracheobronchales for porcine circovirus-2 (PCV2) seropositive and seronegative pigs from two Latvian farms. The research was carried out in 2009. Two Latvian farms were investigated – conditional farm A in Latgale, and farm B in Kurzeme. Pigs from farm A had never been vaccinated against PCV2, but on farm B the PCV2 vaccination programme had been stopped six months ago. Ten pigs, 5-15 weeks old, were selected from each farm for investigation. Generally, four lymph nodes were taken from each animal. The blood serum of all 20 pigs was serologically tested for the PCV2 antibody of ELISA Synbiotics, Serelisa PCV2 Ab Mono Blocking kits. Four structural parameters of a lymph node were detected on histological slides: visualization of follicle structure, lymphocyte depletion, amount of histiocytic cell infiltration, and amount of multinucleate giant cell infiltration and localization in the lymph node. Only in lymph nodes of PCV2 seropositive pigs multinucleate giant cells were detected, which is an important additional parameter for serological test and is suggestive of PCV2 infection. As a result, an interrelation between the amount of histiocytic cells and the follicle structure was detected in pigs' lymph nodes: the more histiocytic cells in the lymph node, the greater the loss of the lymphoid follicle structure.

High economic losses in agriculture can be caused by pig disease like Postweaning Multisystemic Wasting Syndrome (PMWS). Porcine circovirus-2 (PCV2) is the primary agent of PMWS. It is known that PMWS causes cellular and humoral immunity disorders, therefore it is considered a general immune deficiency disease in piglets. It is still unknown, why piglets with PMWS have severe loss of lymphocytes in lymphoid tissues and in the blood. Thus, the hypothesis of the study was that lymphocyte depletion occurs because of lymphocyte life-cycle delay in pigs infected by PCV2. The activity of cell life-cycle is attributed to protein Ki67. This protein is found in all active phases of the cell like interphase G1, S, G2 and mitoses. However, protein Ki67 is not observed at all in the cell’s “quiet” phase G0. The aim of our work was to investigate the lymph node cell proliferation activity of pigs infected by PCV2. In total, 42 pig carcasses were collected from 5 to 15 weeks old pigs with PMWS clinical manifestation. Lymph nodes were selected from each pig carcass for histological and immunohistochemical testing. We concluded that B lymphocyte population was reduced first in the lymph node with PCV2, but the number of T lymphocytes decreased later. PCV2 decreased B lymphocyte proliferation activity.

The research paper focuses on description of the pig (Sus scrofa domestica) farming tendencies in Latvia with the scope to give feeding characteristics in relation to emission outcome. In the recent years the concentration of pigs in farms with a herd size more than 10,000 has increased. With increase of the large farms the average feed consumption is more affected by one operator. Efficient utilization of nutrients content in feeding is crucial to meet environmental goals. It is one of the steps to achieve Nutrient Use Efficiency. There is relationship between crude protein and reduction of reactive nitrogen. Feed content not only affects excreted nitrogen, but also pH of manure and total ammonia Cal nitrogen. It is complex abatement measure to quantify reduction of the emission because of impact of various environmental factors. In Latvia, the most common are sows cross breeds (Yorkshire × Landrace) and on January 1, 2019 the biggest group accounted was fattening pigs with average dry feed consumption per day from 0.33 kg (live weight from 5 to 6 kg) up to 3.6 kg (live weight from 80 to 120 kg) with crude protein value from 163.5 g to 155.3 g per 1 kg feed dry matter. Excreted nitrogen (Nex) was calculated for these groups of pigs less than suggested values in guidelines for emission calculation. The highest calculated total NH3 emission is from fattening pigs group with live weight from 55 to 90 kg although the highest calculated Nex is for lactating sows. NOx also calculates as NO2 and the highest value was 3.23 g per one lactating sow.