The present study was undertaken to estimate enzymatically hydrolysed and non–hydrolysed wheat (Triticum aestivum) and rye (Secale cereale) bran microflora. Enzymatic hydrolysis was accomplished by α – amylase from Bacillus amyloliquefaciens and by Viscozyme L which contain a wide range of enzymes responsible for the breakdown of carbohydrates into simple sugars. Wheat and rye bran samples were collected from native mills, namely Stock Company (SC) ‘Rigas dzirnavnieks’ wheat bran with large particle size (WLSR), SC ‘Jelgavas dzirnavas’ rye bran with small particle size (RSSJ), SC ‘Dobeles dzirnavnieks’ wheat bran with small particle size (WSSD) and wheat bran with large particle size (WLSD). Gained results indicate that before enzymatic hydrolysis all of the bran samples showed similar microbiological contamination with total plate count (TPC), yeasts and lactic acid bacteria. Enzymatic hydrolysis of bran gives the possibility to partially eliminate the microbiological contamination with TPC, yeasts and lactic acid bacteria. The amount of microorganisms after enzymatic hydrolysis (before storage) were decreased and ranged from 5.26 ± 0.04 to 5.45 ± 0.01 log CFU gE-1, from 4.81 ± 0.01 to 5.60 ± 0.05 log CFU gE-1, and from 4.09 ± 0.01 to 5.10 ± 0.05 log CFU gE-1, respectively. After eight weeks of storage (temperature – 20 ± 1 °C, relative humidity – 40 ± 1%) enumeration of microorganisms showed significant decrease of colony–forming units in all bran samples. The amount of TPC, yeasts and lactic acid bacteria in the control bran samples fluctuated in a range from 4.84 ± 0.04 to 5.49 ± 0.05 log CFU gE-1, from 4.86 ± 0.03 to 5.25 ± 0.03 log CFU g-1, 3.53 ± 0.03 to 4.21 ± 0.02 log CFU gE-1 respectively.

Cereal and whole grain products are an important source of dietary fiber in the human diet. Since the milling process highly influences the proportions of the different cell types in the bran, it is expected that brans originating from different genetic/agricultural backgrounds and produced by different processes have different chemical composition. Wheat (Triticum aestivum) bran has not been studied in Latvia, therefore the aim of the present study was to analyze physical – chemical characteristics of wheat bran from Latvia’s industrial mills. Four bran samples were collected from two industrial mills: Stock Company ‘Dobeles dzirnavnieks’ and SC ‘Rigas dzirnavnieks’. All experiments were performed at the Food Technology of Latvia University of Agriculture in May 2011-February 2012. The particle size distribution of the bran samples was determined by sieving. The content of titratable acidity (TA) was detected by titration with 0.1 M NaOH till pH 8.5 was stable for 1 minute. Wheat bran colour was analysed by Hunter Lab colour measurement instrument using ColorTec colorimeter PCM/PSM in CIE L * a * b* system. Moisture content was analyzed using standard ICC 110/1 method by sample drying for 2 h at 150 °C. Results showed that there were significant differences between varieties (p0.05) for titratable acidity and for particle size, but no significant differences (p0.05) were found between the varieties in moisture, pH and colour. TA varied from 6.40±0.71 to 12.05±0.21°, moisture content- from 10.01±0.51% to 11.61±0.47%, pH–from 6.31±0.61 to 6.80±0.05, but colour of bran between the samples did not significantly differ.

The purpose of this study was to evaluate the effect of probiotics, herbals and buckwheat bran (Fagopyrum esculentum L.) on growth, profile of blood, gut microbiota, profile of fatty acid in meat and shedding of resistant Escherichia coli (E. coli) in piglets. A total of 44 piglets (Sus scrofa domesticus) from age of day 14 to 56 were divided into 4 groups. Control received basal diet (group C), basal diet + probiotics (group P), basal diet + 3% buckwheat bran (group PB) and basal diet + 1.5% herbals (group H). No effect was observed in growth in all groups. The count of Lactobacillus spp. increased (p is less than 0.05) in jejunum in group P. In the faeces, Enterobacteriaceae decreased in the group P (p is less than 0.05) of 35 days old piglets, but Enterobacteriaceae and E. coli decreased in the group H (p is less than 0.05) of 56 days old piglets. The prevalence of resistance to at least one antibiotic class was 66.7% before and 50% after the experiment in all groups. Multidrug resistance of E. coli was not observed in 14 days old piglets, but was observed in 50% and more in all of study groups of 56 days old piglets. The fatty acid composition of Longissimus thoracis muscle had higher levels of α-linolenic acid and palmitoleic acid (p is less than 0.05), but lower level of stearic acid (p is less than 0.05) in group P. In conclusion, probiotics and herbals improved gut microbiota, fatty acid profile and affected shedding of resistant E. coli, but not growth performance.