The objectives of the investigation were to determine effect of hull-less gene on grain quality traits (crude protein, starch and β-glucans) and to evaluate the heritability of these traits in the F4 and F5 generations of six crosses between covered and hull-less barley (Hordeum vulgare L.). The trial was carried out at the State Stende Cereal Breeding Institute during the years 2007 and 2008. Mean value, range and the coefficient of variation of traits for covered and hull-less lines within each cross combination were calculated. Heritability of quality traits was estimated as intergeneration correlation (r) and linear regression coefficients (b). The highest intergeneration correlation values were observed for starch content within covered and hull-less barley populations (rF4:F5=0.609 and 0.635 respectively). Moderate intergeneration relationship was observed for crude protein content within covered barley population (r=0.582), and for β-glucans content within hull-less barley population (r=0.593). The regression estimates of heritability were higher for starch content for both covered and hull-less barleys (b=0.549 and 0.703 respectively). Due to effects of genotype and environment interactions the regression estimates of heritability for crude protein and β-glucans content for both covered and hull-less barley were low (b varied from 0.347 to 0.373).

The EU Directive on the promotion of the use of biofuels or other renewable fuels for transport (CO M 2003/30/EC) states that the proportion of biofuels in 2010 must make 5.75% of non-renewable fuels, but in 2020 - 10%. The only way for Latvia to fulfil the Directive 2003/30/EC is to use winter rye because it is one of the oldest cereals grown in Latvia, and Latvia has a long-lasting experience in growing it. A field trial of winter rye (Secale cereale L.) was arranged in the Agricultural Science Centre of Latgale from 2005 to 2007. The influence of four winter rye varieties and five fertilizer rates on grain yield and starch content was researched for three years. The year (factor A), the varieties (factor B), the fertilizer rates (factor C), and the interaction of factors AB, AC , BC, and ABC significantly influenced the yield of grain and the starch content with 95% probability. It is recommended to use good quality grain with high starch content and low protein content for bioethanol production. The varieties of winter rye 'Kaupo', 'Amilo', and 'Walet' are the most suitable for ethanol production in Latgale region.

The effect of dietary supplement with 17% high-amylose barley (Hordeum vulgare, HAB) 'Karmose' on boar taint was studied on 72 crossbred entire male pigs (Sus scrofa domestica) Swedish Yorkshire dams x Swedish Landrace sires). All pigs were fed the same commercial diet (12.4 MJ metabolised energy (ME) per kg, digestible crude protein (CP) 13.5%) twice a day until the average live weight (LW) in the pen reached 100 +- 11.8 kg. Then, 32 out of 72 pigs received an HAB diet. Half of these pigs received HAB for 14 days and the other half for 28 days. The pigs were slaughtered at an average LW of 122.3 +- 8.6 kg (age 167.2 +- 7.5 days). The HAB diet did not affect performance, carcass and meat quality characteristics, and puberty status, but reduced plasma skatole levels in the pigs (least squares means, 3.6 vs. 2.0 ng mlE-1, P = 0.037). However, this diet failed to reduce skatole, indole and androstenone levels in fat (P is greater than 0.10). Nevertheless, it is desirable to continue investigation of HAB to optimise the feeding procedure to improve efficiency of HAB effect on fat skatole levels. Additionally, validation of a greater amount of HAB in the diet should be performed.

The main purpose of the research was to investigate the physically-chemical parameters of organic and conventional hull-less barley (Hordeum vulgare) harvested in Latvia. In the experiment the following hull-less barley harvested in 2012 from the State Priekuli Plant Breeding Institute (Latvia) was used: ‘Irbe’, PR4651, PR3808.21 and PR5099. The following quality parameters of grain – protein, starch, the bulk density and β-glucan content – were measured by standard methods – Infratec™ 1241 Grain Analyser (Denmark) corresponding to ISO 12099; thousand-grain weight (TGW) (ISO 520:2010); the falling number (ISO 3093:2009); moisture content (LVS 272:2000) were also determined. In the present experiments no significant differences in protein, moisture, starch, β-glucan content and TGW were detected, however, significant differences in the bulk density of all analyzed hull-less barley samples were established. The lower analyzed parameter value of 780 ± 1 g LE-1 was obtained for the conventional and organic hull-less barley PR 5099. However, the higher bulk density value of 821 ± 1 g LE-1 was obtained in the conventional hull-less barley variety ‘Irbe’. No significant differences in the bulk density of conventional and organic hull-less barley ‘Irbe’, PR4651 and PR3808.21 were found. Significant differences in the falling number were detected in the analyzed hull-less barley samples harvested in the conventional and organic farming. The lower falling number value was obtained in the organic hull-less barley line PR 5009 – 81 ± 4 s, the higher in the conventional and organic hull-less barley line PR 3808.21 as 362 ± 5 s and 373 ± 2 s, respectively.

Renewable energy resources play an important role in energy production both in Latvia and in the world. Bio-ethanol is used as a substitute for oil products in various countries of the world. It is produced from the plants containing starch: cereals, potatoes, beet, maize. The task of the research was to evaluate the suitability of different varieties of winter wheat, triticale and rye for extraction of bio-ethanol in Latvia. The research was carried out at the State Stende Cereals Breeding Institute in 2009, and the following varieties and lines of cereals were examined: winter wheat varieties 'Mulan', 'Skalmeje', and the line '99-115', developed at the State Stende Cereals Breeding Institute; winter triticale varieties 'SW Valentino', 'Dinaro', and the line '0002-26', developed at the State Priekuli Plant Breeding Institute; winter rye varieties 'Matador', 'Placido' Fp and 'Dankowskie Nowe'. The content of crude protein and starch of grains, the grain yield, and the bio-ethanol yield were determined. The highest bio-ethanol yield was acquired from the winter wheat and triticale varieties that had the highest starch content and the lowest crude protein content of grains. The best results were obtained from winter wheat line '99-115' and variety 'Mulan' (409.4 and 406.8 L tE-1), triticale variety 'Dinaro' (423.3 L tE-1), and winter rye variety 'Matador' (370.1 L tE-1.).

Bioethanol can be used for food production and to partially replace fossil fuel. Bioethanol is mainly produced from renewable biomass that contains sugars, starch or lignocellulose. The main raw materials for production of bioethanol are cereals, maize (Zea mays), sugarbeets (Beta vulgaris sacccharifera) and other plant species. During the trial that took place in State Stende Cereals Breeding Institute during a three year period (from 2009/2010 to 2011/2012) we examined the suitability of grain from winter wheat (Triticum aestivum L.), triticale (×Triticosecale Wittm) and rye (Secale cereale L.) for the production of bioethanol. Three varieties of each species were used in the trial. During the trial period the grain yield, the ethanol outcome (L tE-1) and the ethanol yield (L haE-1) were determined. It was established that during three years wheat and triticale provided the highest starch content (more than 700 g kgE-1) of the grain as well as the highest ethanol outcome (L tE-1). These species provided both high grain yield (more than 9 t haE-1 on average) and the highest ethanol yield (3300 – 4665 L haE-1). The choice of variety was also important as both the grain starch content and the grain yield depend on the genotype of the variety.

The experiment was carried out in the Latvia University of Agriculture. The objectives of this research were to study changes of bean (Phaseolus) protein fractions occurring under the thermal treatment conditions and determine the critical temperature for Maillard reactions in beans. In these reactions lignified protein is made from amino acids and sugars, and it decreases the nutrition value of the food. If lignified protein exceeds 50% of crude protein content in food, then food is considered unsuitable for daily diet. In this experiment beans were milled and then heated at 50 ± 3 °C, 75 ± 3 °C, 100 ± 3 °C, 125 ± 3 °C and 150 ± 3 °C temperature in the drying oven for 20 h. As a control sample non heated beans were used, and they all were kept in a plastic jar at room temperature (20 ± 1 °C). Dry matter, crude protein, starch, sugar and lignified protein content were determined in heated beans and control samples. No significant changes of crude protein content were observed due to thermal treatment. It was from 24.3 ± 0.3 g 100 g-1 of dry matter. Similarly, no significant changes were observed in starch content, as it stayed averagely 47.1 ± 0.2 g 100 g-1 of dry matter. Medium high correlation was observed (0.64) between lignified protein content and sugar content for in different temperature treated beans. Lignified protein showed exponential growth if the samples were heated at a temperature of 100 ± 3 °C and higher, giving exponential change.

Oat breeders have improved yielding ability potential of oat (Avena sativa L.) cultivars, but set lower standards for biochemical composition of grain. Nowadays the quality of grain for consumers has become important especially in terms of lipids and β-glucan content. Experiments were carried out at State Stende Cereal Breading Institute in the year 2012 to evaluate the oats yield and grain quality. 15 different cultivars of Stende collection were compared by yield and parameters of productivity (test weight and 1000 kernel weight) as well as biochemical parameters (protein, starch, lipid, β-glucan, content). Biochemical parameters were tested by Infratec Analyser 1241. To obtain an equal research background all cultivars were grown in a plant breeding crop rotation field, with similar growing conditions (sowing-time, fertilizer, plant protection activities), which agree with generally accepted technology of oat cultivation in Latvia. Experiments were done in four replications. Plots were laid randomized. ANOVA procedures were used for data analysis. Yield of experimental cultivars varied between 4.28 ± 0.19 - 5.93 ± 0.22 t haE-1, test weight 46.85 ± 0.99 - 52.75 ± 0.44 kg hLE-1, 1000 kernel weight 33.70 ± 0.24 - 46.34 ± 0.80 g. Significant differences of tested yield parameters among oat cultivars were observed. The highest protein content was observed for local breed cultivars ‘Arta’ and ‘Mara’ 119.5 ± 02.6 and 110.5 ± 1.9 g kgE-1 accordingly. Low starch content, but high β-glucan content is characteristic for all Latvian cultivars.

The objective of the current research was to investigate effects of germination on chemical composition of hull-less barley (Hordeum vulgare L. var. nudum Hook. f.), hull-less oat (Avena sativa), rye (Secale spp.), and wheat (Triticum spp.) grains for comparison. All the grains were cleaned, washed, steeped and germinated at temperature 35 ± 2 °C and relative humidity 95 ± 2% for 12, 24, 36, and 48 hours. After germination grains were dried till moisture content 14 ± 2%. Main quality parameters such as starch, proteins, b-glucan (in hull-less barley) and individual sugars were determined in cereals during their steeping and germination. Non-germinated grains were used as a control sample. In the present experiments non-significant protein content increase was observed in the analysed hull-less barley, hull-less oat, rye, and wheat during their germination for 48 hours. Starch content in hull-less barley, wheat and rye grains decreased non-significantly during germination for 24 h; opposite results were obtained for hull-less oat grains, where content of starch decreased by 16.7% after steeping and by 26.4% after germination for 24 h. b-glucan content in hull-less barley grains after germination for 48 h decreased by 20.5%. Non-significant changes were obtained in fructose content in analysed cereal grains during germination for 48 h; it was significantly increased after germination for 24 h and in germination for 48 h. Non-significant sucrose content changes were observed in hull-less barley, rye and wheat grains during germination for 12 h and in hull-less oat grains – for 24 h significantly increasing in future germination for 48 h.

The present study provides research in content of protein, starch, sugars and concentration of B group vitamins (thiamine, riboflavin, pyridoxine and niacin) in immature wheat (Triticum aestivum L.) compared to mature wheat kernels. The content of protein, starch was determined in kernels using Infratec1241 Grain Analyzer (Sweden), content of sugars was analysed by high- efficiency liquid chromatography HPLC (Shimadzu, Japan). The concentrations of vitamins were determined by high-efficiency liquid chromatography for vitamins B1, B2, B3, B6. Obtained results suggested that the immature wheat kernels obtained in milk stage showed higher contents of some compounds such as sugars and B group vitamins. The amount of all sugars presented in immature grains is higher and decreased uniformly during maturation. Therefore, immature grains are better sources of the B group vitamin that the mature grains, and therefore more nutritionally efficacious.