In recent years, the effective immunostimulating properties of β-glucans have been widely proven not only in mammals, but also in aquatic animals. The aim of this study was to determine the effect of dietary β-glucan supplements on the percentage of individual types of leukocytes and the haematocrit of the sea trout (Salmo trutta L.). Investigations were performed at the state fish farm ‘Tome’, hatchery ‘Pelči’ of the Institute of Food Safety, Animal Health and Environment ‘BIOR’, during a six month period (September 2018 – February 2019). On the basis of feeding with immunostimulant diets, a total of 15 000 sea trout were assigned into five groups. For blood collection healthy fish were randomly selected once a month, five 5 individuals from each group. Blood smears were made to determine the leukocyte differential count. Sampling was done once a month (September 2018 – January 2019) (n=125). Haematocrit was determined by the standard microhaematocrit method. Sampling was done once a month (January 2019 – February 2019) (n=50). We concluded that the dietary β-glucan supplements can improve the haematocrit level and effect the percentage of individual types of leukocytes of the sea trout, stimulation of the preparation for parr-smolt transformation.

The aim of this pilot study was to assess whether a single mesenchymal stem cell intra-articular injection in the osteoarthritic joint gives a therapeutic effect. This trial study was made in order to better manage and structure further study on more dogs. Single injections of 3 million mesenchymal stem cell intra-articular injections were made bilaterally in the osteoarthritic elbow joints. The dog was examined before and after monthly stem cell therapy using stance analyser scales to determine the static weight of each limb. Weight balance between forelegs improved, but not significantly (p is greater than 0.05). A radiographic examination was made for both elbow joints before stem cell therapy and the second and fifth month thereafter. Calculations of sub-trochlear sclerosis in percentage were made using mediolateral projections. Sub-trochlear sclerosis in percentage significantly decreased (p is less than 0.05) after stem cell therapy in both legs.

Parafasciolopsosis is a parasitological disease which is caused by the liver fluke Parafasciolopsis fasciolaemorpha. This parasite which belongs to herbivores often causes invasion in elks (Alces alces). The aim of our study was to diagnose the parasite and investigate what kind of histopathological changes it caused in the liver tissue. The study took place in Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Laboratory of Comparative Pathology and Laboratory of Parasitology. During the study from 2017 to 2018 we collected liver samples and faeces from 46 felled elks of different age and gender, from all over Latvia. Ten grams of faeces were examined for trematode eggs by sedimentation method and a section of liver was examined for the presence of flukes and the histological structure of the liver. In this study Parafasciolopsis fasciolaemorpha were found in 11 samples of liver from 46 elks, with invasion extensity of 24%. The histopathological examination of the liver samples revealed fibrosis, proliferation of bile ducts, pericholangitis, calcerous deposits in bile ducts and other notable histological changes in the liver tissue that can cause hepatic trematodes.

The feature of the microorganisms of rumen microbiocenosis is the ability to form a number of digestive enzymes, including cellulases, which allows ruminants use the energy of feeds rich in fiber. Microorganisms that are promising as a source of cellulases, biodestructors of toxins having antagonistic properties against pathogens for creating probiotic feed additives, both for reindeer and for other livestock, were isolated from the reindeer rumen. The aim of the study was to create a collection of microorganisms that are promising for creating a biopreparation. As a result, collections of 63 associations of microorganisms decomposing cellulose and carboxymethylcellulose were created; their morphology and cultural properties were described. It was found that the amount of cellulose degradation was 44 – 62% in the 4 most active isolates (No 14, 15, 21 and 26). The obtained data allowed to make a conclusion about the ability of bacterial strains isolated from the rumen to synthesize cellulolytic enzymes carrying out cellulose biodegradation, which allows them to gain a competitive advantage in the rumen of the reindeer diets with an abundance of cellulose. Isolates No 14 and 15 showed high antagonistic activity to Fusarium sporotrichioides, and isolate No 15 and 16 showed high antagonistic activity to Fusarium oxysporum. Isolate No 15 showed a wider antifungal activity compared to other isolates. Among all isolated strains, this bacterial isolate is represented as having a large spectrum of activities, including both high cellulolytic and high antifingal activity.

The assessment and conservation of agricultural biodiversity is one of the current goals of European Union policies, which are reflected in European Biodiversity Strategy. The biodiversity of soil seed bank and above-ground weed flora were investigated in spring cereals in organic and conventional farming system. The study was carried out between 2012 and 2014. Species of above-ground wild flora were counted on a surface of 1 square m in the field. Soil for seed bank evaluation was taken from the field and evaluated by seedling emergence method in a greenhouse. There were no significant differences in number of species between two communities. Biodiversity of weed community, described by Shannon’s diversity index and Simpson’s dominance index, was significantly better maintained in the soil seed bank than in above-ground flora in both farming systems. A strong, positive correlation between the number of species, Shannon’s diversity index, Simpson’s dominance index of soil seed bank and above-ground weed species was found in both farming systems, while abundance of seeds and above-ground weeds was significantly, positively correlated only in the conventional farming system. Qualitative composition of weed flora depended more on the type of community (soil seed bank or above-ground weed communities) than the farming system.

The impacts of agricultural policies are decisive on the agricultural sector, especially in the case of the European Union’s (EU) Common Agricultural Policy (CAP). The 2013 CAP reform introduced several new measures. In budgetary terms, basic payment and greening were the most significant ones. Besides, coupled supports, young farmer and small farmers scheme should be mentioned. To reveal the changes caused by these measures, the time horizon of the study is 2013−2017. Since the subsidies represent a significant part of the income generated in the agricultural sector, the basic hypothesis of the study is that the production units concerned (individual farms, private enterprises) gave economically rational answers. In practice, this means trying to fully adapt to the changes to maximize support. Based on the analysed datasets (Hungarian Central Statistical Office and Hungarian State Treasury), it became evident that farmers responded rationally: splitting up farms against capping, moving towards the lowest resistance to comply with greening, plus associating them with the extra support of coupled payments. Generational renewal is a key issue; however, the present form of young farmers’ scheme needs to be refined to be more effective. Small farmers scheme is a good opportunity for farmers with less than 5.5 ha and most of the concerned farmers chose well. However, there are about 6,000 farms that are too small for the standard system, while 772 farms that are too large for the small farmers scheme.

Winter oilseed rape is the main oilseed crop in Latvia. High yield production depends on applied plant nutrients, especially nitrogen. Nitrogen is one of the most mobile plant nutrients in the soil and, therefore, nitrogen fertilizer management is an important part of agriculture. The objective of this paper is to evaluate the effect of nitrogen fertilizer rate on nitrogen use efficiency (NUE) in winter oilseed rape. The field experiment was conducted during four growing seasons (2014/2015, 2015/2016, 2016/2017 and 2017/2018) at the Research and Study farm ‘Pēterlauki’ of Latvia University of Life Sciences and Technologies (56° 30.658´ N and 23° 41.580´ E). In total, eight nitrogen rates were used in this experiment (kg ha-1 of N pure matter): N0 or control, N60, N90, N120 (80+40), N150 (100+50), N180 (120+60), N210 (120+60+30) and N240 (140+60+40). All NUE parameters were calculated only for five nitrogen rates (N0; N60, N120, N180 and N 240). Results indicated that the nitrogen fertilizer rate had a significant (p is less than 0.001) effect on winter oilseed rape seed yield. The yield increased until the rate N120 – N180. Nitrogen fertilizer rate also significantly (p is less than 0.001) affected N, P2 O5 and K2 O removal with oilseed rape biomass. N and K2 O removal with seed yield significantly increased until the nitrogen rate N120 – N180, but P2 O5 until N60 – N180. Results showed that the nitrogen fertilizer significantly (p is less than 0.001) affected NUE, nitrogen uptake efficiency (NUpE) and nitrogen utilization efficiency (NUtE), but it did not have a significant (p=0.840) effect on nitrogen harvesting index (NHI). By increasing the nitrogen fertilizer rate, NUE, NUpE and NUtE decreased.

New types of polymer fertilizer series ‘Vitanoll’ are used in adaptive-varietal agro technology in the cultivation of seed potatoes. The experiments were conducted on sod-podzolic soils, typical of the North-West region of the Russian Federation. The aim of the research was to study the effect of new polymer fertilizers on potato yield. Research tasks included assessing the effect of various polymer fertilizers (Polymer-N, Polymer-P, Polymer-K.), as well as the effect of Polymer-N on different potato varieties. Based on the studies conducted, the most responsive potato varieties to polymeric fertilizers with nitrogen were identified. They turned out to be Salin (increase of 7.1 t haE−1) and Mocart (increase of 4.1 t haE−1). Evaluation of the influence of different types of polymer fertilizers revealed that the most effective are polymer fertilizers with potassium.

The aim of the work was to study accessions of Dactylis glomerata L. of different origin for breeding in the Leningrad region. The work was carried out at the Leningrad Agricultural Research Institute. The study involved 15 accessions. The nursery was founded in 2016 and included two replications for green yield and two – for seed production. Cutting and sampling for biochemical studies were conducted in May-June 2018, seeds were collected in July. The amount of ascorbic acid, carotenoids and chlorophylls and crude protein were determined. Statistical analysis included the calculation of the parameters of variation, ANOVA and correlation analysis. Three groups were identified by the degree of ripeness: a small group of early maturing – 31 days, a group of late maturing (single accession from Karelia) – 51 days, a large group of middle maturing – 34–37 days. For the late maturing accession the mechanism of regulation of the beginning of heading is due to the sensitivity to photoperiod. The adaptive potential of widely zoned varieties is wider than for localized. Their productivity is higher. The content of pigments in the green mass is closely correlated. The system of pigments in the complex of characters is separate, but is positively associated with the seed productivity and negatively – with the content of ascorbic acid. The seed productivity of the accessions is related to the total pigment content and power of the generative shoots (length and mass).

Public participation is needed for nongovernmental organisations (NGO) to function and exist, as the public plays the role of investors by supporting the NGOs financially and thereby providing a quorum needed for the organisation to operate and make decisions. NGOs provide services for defending public interests, and the NGOs members carefully assess their gains from involvement or participation continuation in public organisations, just as if choosing a good or service for consumption. The level of society activity in NGOs sector is low in Latvia comparing with the Western world. To increase it, it is required to contribute to the public’s trust and NGOs transparency. Organisational performance analysis is one of the ways how to provide members with information on performance and funding use, as the members of NGOs wish to be sure that their membership fees and other financial support are effectively used to achieve their goals and defend the interests of their members. The research aims to analyse financial analysis methods used in the NGO sector. The research tasks were set: 1) to analyse the substance of financial analysis of NGO, and 2) to identify shortcomings for a comprehensive analysis of NGO. The research has found that as public participation in NGOs increases, it becomes increasingly important to provide financial transparency in the NGO sector in order to contribute to the public’s trust in the NGOs and make it possible to verify the consistency of funding use with the organisation’s goals and performance.