Bu çalışmada, Gundellia tournefortii bitkisinin tohumlarından elde edilen su ekstraktının GC/MS (Gaz kromatografisi -Kütle spektrometresi) analizi ile genel olarak içeriğinin değerlendirilmesi, makro ve mikro besin elementi konsantrasyonları, antimikrobiyal aktivitesi, toplam antioksidan seviyesi (TAS), toplam oksidan seviyesi (TOS), oksidatif stres indeksi (OSI) değerlerinin belirlenmesi ve çeşitli hücre hatları üzerindeki anti-kanserojen niteliklerinin ortaya konulması amaçlanmıştır. TAS, TOS ve OSI değerlerini tespit etmek için Rel Assay Diagnostics kitler kullanılmıştır. Antimikrobiyal aktivite analizleri, Mikrodilüsyon Broth yöntemi ile Minimum inhibisyon konsantrasyon (MIC) değeri belirlenerek, Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Bacillus cereus (ATCC11778), Klebsiella pneumonia (ATCC 13883), Candida albicans (ATCC 10231) ve Candida tropicalis (DSM11953) mikroorganizmaları üzerinde yapılmıştır. Sitotoksik aktivite ise, normal fare fibroblast hücre hattı, HUVEC ve 3 farklı insan kanser hücre hattında MTT yöntemi ile belirlenmiştir. Sonuç olarak, Gundelia tournefortii bitki ekstraktının S. aureus bakterisi üzerinde orta düzeyde, diğer mikroorganizmalar üzerinde ise zayıf düzeyde antimikrobiyal aktivite, iyi derecede antioksidan aktivite ve bazı hücrelerde sitotoksik aktivitesinin olduğu tespit edilmiştir. Bitkinin oksidatif stres indeksinin ise, düşük olduğu belirlenmiştir. Ayrıca, makro ve mikro besin elementi içeriği bakımından Gundellia tournefortii bitkisinin %3,64 N, %0,11 P, %3,78 K, %0,22 Ca, %0,57 Mg, 268,4 mg/kg Fe, 16,7 mg/kg Zn, 19,4 mg/kg Mn ve 8,3 mg/kg Cu konsantrasyonlarına sahip olduğu belirlenmiştir.

Antioxidants, usually contain phenolic groups, molecules may prevent the formation of free radicals or by blocking the radicals to prevent damage to the cell. Antioxidant molecules can be produced by the body or taken into the body through foods. Today, there are many different methods used to measure antioxidant capacity. In these methods, measurements are usually carried out by adding the tested substances into a solution containing free radicals. These methods can give different results depending on the characteristics of the reactant used. In this study, the reproducibility and consistency of five different antioxidant capacity methods (DPPH, ABTS, galvinoxil, phosphomolybdenum and FRAP) in commercially available and homemade turnip samples were tested. At the end of the study, antioxidant activities of the tested samples were evaluated. According to the evaluation of the antioxidant activities (from higher to lower) of these samples, the results of, DPPH, galvinoxyl, phosphomolybdenum and FRAP methods were very similar. However, ABTS method results showed difference compared to other method results. As a result of study, reproducibility and consistency of results of DPPH, galvinoxyl, phosphomolybdenum and FRAP methods were determined.

Garlic (Allium sativum L.) is a vegetable that is known to be medically beneficial and has been around since the past in the world cuisine. The unique taste and smell of garlic affects its raw consumption negatively. One of the functional uses of garlic, ‘black garlic’ is produced by fermenting white garlic under certain temperature and humidity conditions. Thus, the bitter taste and smell of garlic disappears and also changes occur in its nutritional value. Studies show that black garlic has anticancer, antitumor, antiallergic and antimicrobial effects. It also plays protective and therapeutic role against diabetes, obesity, cardiovascular diseases. This product, which is new for many countries, is not well known and consumed in our country too. In this study, the bioavailability of black garlic was highlighted and information was given about its production and antioxidant activity.