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Histopathological Research of the Therapeutic Effects of Grape (Vitis vinifera L.) Seeds Extracts on Cadmium-Exposed Carp (Cyprinus carpio L. 1758) Texte intégral
2022
Nuh Korkmaz
Histopathological Research of the Therapeutic Effects of Grape (Vitis vinifera L.) Seeds Extracts on Cadmium-Exposed Carp (Cyprinus carpio L. 1758) Texte intégral
2022
Nuh Korkmaz
This study was designed to investigate the therapeutic effects of Vitis vinifera L. seed extract (5 ppm and 10 ppm) on gill and liver tissues histopathology of carp (Cyprinus carpio L. 1758) exposed to cadmium chloride (CdCl2=Cd) (20 ppb) acute and subchronic period. A total of 140 fish in 14 groups were used in the experiment. The experiment was designed as in two different periods (acute and chronic) and 7 different dose groups (control, vehicle, V. vinifera seed extract 5 ppm, V. vinifera seed extract 10 ppm, Cd 20 ppb, V. vinifera seed extract 5ppm + Cd 20 ppb and V. vinifera seed extract 10 ppm + Cd 20 ppb). At the end of the experiment, the gill and liver tissues dissected from the fish were fixed, dehydrated, cleared, and paraffin impregnated. Appropriately sized blocks were prepared from the tissues, sections of 5-7 µm thickness were taken and covered by staining with hematoxylin-eosin. Stained preparations were examined under a light microscope and photographed. It was determined that all dose groups in the acute period and control groups were not different histopathological. It was determined that the histopathological damage caused by the cadmium group in the subchronic period and tissue damage were reduced in the dose groups with cadmium added with V. vinifera seed extract. As a result of the study, it was concluded that V. vinifera seed extract could have a therapeutic effect on the gill and liver tissues of carp exposed to cadmium.
Afficher plus [+] Moins [-]Germination of <i>Chloris barbata</i>, <i>Cynodon dactylon</i>, and <i>Cyperus rotundus</i> from Angola at Constant and Alternate Temperatures Texte intégral
2019
Loddo, Donato | Carlesi, Stefano | Pais da Cunha, Adérito Tomás
The study of weed germination can improve knowledge on their seasonal dynamics in fields and facilitate the design of ecologically sustainable management. An experiment was conducted to evaluate the effect of a range of constant and alternate temperatures on the germination of Chloris barbata, Cynodon dactylon, and Cyperus rotundus collected in Angola. Interesting differences were observed between the germination responses of the three species. No germination was observed for any species at 10 or 15–5 °C. Chloris barbata quickly germinated at most constant and alternate incubation temperatures, showing low primary dormancy and high germinability. Fast and high germination was observed for Cynodon dactylon at most alternate temperatures, while lower germination percentages were achieved at constant temperatures, especially the highest ones. Cyperus rotundus achieved good germination percentage at alternate temperatures, even if with slower and more extended germination dynamics than the other species, while almost no seeds germinated at constant temperatures. This study improves our knowledge of germination ecology for these species and allows some control tools to be identified, such as soil tillage, cover crops, and living or dead mulches, which can contribute to their ecologically sustainable management.
Afficher plus [+] Moins [-]Histopathological Research of the Therapeutic Effects of Grape (Vitis vinifera L.) Seeds Extracts on Cadmium-Exposed Carp (Cyprinus carpio L. 1758) Texte intégral
2022
Nuh Korkmaz
This study was designed to investigate the therapeutic effects of Vitis vinifera L. seed extract (5 ppm and 10 ppm) on gill and liver tissues histopathology of carp (Cyprinus carpio L. 1758) exposed to cadmium chloride (CdCl2=Cd) (20 ppb) acute and subchronic period. A total of 140 fish in 14 groups were used in the experiment. The experiment was designed as in two different periods (acute and chronic) and 7 different dose groups (control, vehicle, V. vinifera seed extract 5 ppm, V. vinifera seed extract 10 ppm, Cd 20 ppb, V. vinifera seed extract 5ppm + Cd 20 ppb and V. vinifera seed extract 10 ppm + Cd 20 ppb). At the end of the experiment, the gill and liver tissues dissected from the fish were fixed, dehydrated, cleared, and paraffin impregnated. Appropriately sized blocks were prepared from the tissues, sections of 5-7 µm thickness were taken and covered by staining with hematoxylin-eosin. Stained preparations were examined under a light microscope and photographed. It was determined that all dose groups in the acute period and control groups were not different histopathological. It was determined that the histopathological damage caused by the cadmium group in the subchronic period and tissue damage were reduced in the dose groups with cadmium added with V. vinifera seed extract. As a result of the study, it was concluded that V. vinifera seed extract could have a therapeutic effect on the gill and liver tissues of carp exposed to cadmium.
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