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The Aggregation of Boron on the Tissues of Gold Fish (Carassius auratus Linnaeus, 1758)
2015
Tuncer Okan Genç | Burak Evren İnanan | Murat Yabanlı | Fevzi Yılmaz
In this study, it was aimed to determine the water-borne and food-borne boron accumulation in the liver and muscle tissues of Gold Fish (Carassius auratus Linnaeus, 1758). For each treatment, 12 individuals were. The water-borne boron treatments were applied as boron acid concentration of 1 mg/L, 10 mg/L and 20 mg/L in the aquarium water, while the food-borne boron treatments were prepared food contained the defined levels of boron (1 mg/kg, 5 mg/kg and 10 mg/kg) as boric acid. The boron levels in the tissues were determined by an ICP-MS procedure. The maximum boron concentration was found in the 20mg/L water borne boron treatment in the liver tissue (1.78±0.02 mg/kg). In the water-borne boron treatments, the maximum Transfer Factor (TF) was found in the 20mg/L boron concentration, and TF values were increased when the boron concentrations were decreasing. In the 1 mg/kg food-borne boron treatment, TF was found as 0, and increasing concentration of boron in the food caused an increase in TF reached about 0.06. This study suggested that the target organ for boron accumulation is the liver rather than the muscles and the accumulation of food-borne boron is lower when comparing water-borne boron.
Afficher plus [+] Moins [-]Histopathological Research of the Therapeutic Effects of Grape (Vitis vinifera L.) Seeds Extracts on Cadmium-Exposed Carp (Cyprinus carpio L. 1758)
2022
Nuh Korkmaz
This study was designed to investigate the therapeutic effects of Vitis vinifera L. seed extract (5 ppm and 10 ppm) on gill and liver tissues histopathology of carp (Cyprinus carpio L. 1758) exposed to cadmium chloride (CdCl2=Cd) (20 ppb) acute and subchronic period. A total of 140 fish in 14 groups were used in the experiment. The experiment was designed as in two different periods (acute and chronic) and 7 different dose groups (control, vehicle, V. vinifera seed extract 5 ppm, V. vinifera seed extract 10 ppm, Cd 20 ppb, V. vinifera seed extract 5ppm + Cd 20 ppb and V. vinifera seed extract 10 ppm + Cd 20 ppb). At the end of the experiment, the gill and liver tissues dissected from the fish were fixed, dehydrated, cleared, and paraffin impregnated. Appropriately sized blocks were prepared from the tissues, sections of 5-7 µm thickness were taken and covered by staining with hematoxylin-eosin. Stained preparations were examined under a light microscope and photographed. It was determined that all dose groups in the acute period and control groups were not different histopathological. It was determined that the histopathological damage caused by the cadmium group in the subchronic period and tissue damage were reduced in the dose groups with cadmium added with V. vinifera seed extract. As a result of the study, it was concluded that V. vinifera seed extract could have a therapeutic effect on the gill and liver tissues of carp exposed to cadmium.
Afficher plus [+] Moins [-]Effects of Dietary Supplementation of Some Antioxidants on Liver Antioxidant Status and Plasma Biochemistry Parameters of Heat-Stressed Quail
2017
Senay Sarıca | Hüseyin Aydın | Gulay Ciftci
This study aimed to compare the dietary supplementation of oleuropein (O) and α-tocopherol acetate (TA) alone or with organic selenium (Se) on liver antioxidant status and some plasma biochemistry parameters in Japanese quails reared under heat stress (HS). A total of 800, two-weeks old quails were kept in wire cages in the temperature-controlled rooms at either 22°C or 34°C for 8 h/d and fed on a basal diet (NC) or the diets supplemented with TA (TA200) or O (O200) at 200 mg/kg alone or with OSe (TA200+OSe and O200+OSe) to the NC diet. HS decreased the total antioxidant status (TAS) and increased the total oxidative stress (TOS) and oxidative stress index (OSI) of liver compared to thermoneutral temperature (TN). The TA200, O200, TA200+OSe and O200+OSe diets increased TAS and decreased TOS of liver compared to those of quails fed NC. OSI was decreased by the TA200, O200 and TA200+OSe diets compared to NC and O200+OSe diets. HS reduced plasma albumin (A) and total protein (TP) concentrations, on the other hand, increased plasma glucose (G), total cholesterol (CHO) and triglyceride (TG) levels compared to TN. The TA200, O200, TA200+OSe and O200+OSe diets reduced plasma total CHO and TG levels and increased plasma A level. The TA200 and TA200+OSe diets reduced plasma G level and increased plasma TP levels compared to those of quails fed the other diets. In conclusion, dietary supplementation of vitamin E and oleuropein alone or with organic selenium is necessary to remove the negative effects of heat stress on liver antioxidant status and some plasma parameters of quails.
Afficher plus [+] Moins [-]Effects on Performance, Skin and Liver Histology of Different Clinoptilolite Levels in Rat Diets
2014
Dilek Şentürk Demirel | Ramazan Demirel | Muzaffer Aydın Ketani | Kadri Balcı
The objective of this study was to test the effects of dietary natural zeolite (clinoptilolite) on performance, skin and liver histology in rats. In this study, 24 10-week-old, weaned, adult male Spraque-Dawley rats with approximately 306 +- 18.93 g initial live weight were used. The rats were randomly divided into four groups with three replicates, including a control group and groups with one of three doses of clinoptilolite (2%, 4%, and 6%) in their diets. All the rats were fed these concentrates throughout the experimental period of 56 days. The animals were reared individually in stainless steel cages. There were no significant differences in the primary and secondary follicle numbers among groups, but the diameters of each follicle were found to be significant. The primary and secondary follicle numbers and diameters ranged from lowest to highest as follows: 2.00-2.33, 4.50-7.17; and 11.53-20.42, 57.63-102.12um, respectively. The differences occurred between the control group and group IV (containing 6% zeolite). In addition, the skin and liver histology results showed that there were no differences among the groups.
Afficher plus [+] Moins [-]The Effect of Acetamiprid Administration on Bcl-2 Immunoreactivity in the Liver
2024
Gökhan Nur
This study aimed to show the effect of acetamiprid, a neonicotinoid insecticide, on B-cell lymphoma 2 (Bcl-2) gene expression, which plays an important role in apoptotic mechanisms in liver tissue. The study consisted of four groups in total, in which three doses of acetamiprid (5, 10, and 15 mg kg-1) were administered, together with the negative group, in which no substance was administered. Liver tissues resected from mice sacrificed by cervical dislocation after 14 days of acetamiprid administration by gavage were fixed in a 10% formaldehyde solution for histological and immunohistochemical analyses and blocked in paraffin after routine tissue follow-up, and sections were stained with haematoxylin-eosin and immunostaining. Histological analysis revealed normal liver tissue in the control group; whereas, sinusoidal dilatation, vasodilatation, and necrosis and steatosis in the parenchyma were found in the acetamiprid-treated group at an increasing rate depending on the dose amount. The immunoreactivity of Bcl-2 in liver tissue was observed in the sinusoidal epithelium. Bcl-2 immunoreactivity was observed severely in the control and 5 mg kg-1 groups and moderately in the 10 mg kg-1 and 15 mg kg-1 acetamiprid-treated groups. Bcl-2 immunoreactivity was observed homogenously in the region from the central vein to the Kiernan’s space. It was observed that acetamiprid used in the study showed a toxic effect on liver tissue, affected bcl-2 expression, an important biomarker in apoptotic pathways, and induced a dose-dependent decrease in bcl-2 immunoreactivity.
Afficher plus [+] Moins [-]Comparative Fatty Acid Compositions of Tissues of Rainbow Trout (Oncorhynchus mykiss) with Different Ploidy and Sex
2023
Birol Baki | Dilara Kaya Öztürk
The purpose of this study was to evaluate the fatty acid contents in various tissues (fillet, liver, gonad) of different ploidy (triploid and diploid) and sex (female and male) rainbow trout (Oncorhynchus mykiss) in the breeding season. In the study, diploid and triploid rainbow trouts belonging to the same age group (3+) were used. Fish were fed with commercial feed containing 45% crude protein and 20% crude fat until satiation. At the end of the 75-day study, biometric measurements of the fish were made and the tissues were stored in a deep freezer until biochemical and fatty acid analysis. The first finding of this study identified that ploidy (triploid and diploid) affects the biochemical and fatty acid composition of rainbow trout. The second major finding was that the polyunsaturated fatty acid values were higher and the saturated fatty acid values were lower in all tissues (especially female gonads) than other fatty acids. The results also indicate that the comparative among the biochemical and fatty acid composition of the fillet, liver, and gonad of rainbow trout is further illuminated by these data.
Afficher plus [+] Moins [-]Acute and Subacute Toxicity of Ruta Montana Extract to Female Rats: Effect on Liver, Kidneys and Ovaries
2023
Mahdeb Nadia | Khadidja Attafi | Souha Bouhouhou | Allouni Rima | Abdelouahab Bouzidi
Ruta montana L. is an annual aromatic plant of the family rutaceae. Quantitative analysis of the methanolic crude extract of Ruta montana L. yielded 8.43%, whereas the qualitative analysis revealed the presence of alkaloid or coumarin. The Litchfield and Wilcoxon method calculated the LD50 of the crude methanolic extract of Ruta montana L. in Wistar albino female rats at 393.18 mg/kg. This allows the plant to be classified as moderately toxic. The subacute toxicity study of the methanolic crude extract of Ruta montana L. in female Wistar albino rats treated with 100 mg/kg (≈ 1/4 LD50) and intraperitoneally showed a significant increase in body weight of the rats treated at the 4th week. Animals treated and sacrificed after 30 days showed a disturbance of the relative mass of the organs. Biochemical parameters of hepatic function assessment showed a significant increase in PAL with elevation of AST and ALT, whereas those of renal function revealed a significant decrease in creatinine with an increase in urea. Hematologic parameters recorded a decrease in RBC, HGB and HCT. The histological sections of the treated rats reveal the existence of blood congestion in the central veins and liver tissues, foci of necrosis and steatosis in the liver, blood congestion and some glomerular atrophy in the kidneys, as well as blood congestions and developed follicles without oocytes in the ovaries.
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