Muscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable microclonal propagation protocol for M. neglectum using TDZ (Thidiazuron)-NAA (α Naphthalene acetic acid) to induce bulblets, roots, and acclimatization of the regenerated bulblets. Maximum number of bulblets per explant (8.25±0.05) was noted on MS medium containing 0.0454 µM TDZ-5.37 µM NAA. The bulblets regenerated in each type of culture medium were very vigorous, and acclimatized easily following rooting on a subculture. Here we show that this protocol is a useful clonal micropropagation system for this important ornamental plant.

In this study, rooting performance of cuttings of local olive types of Northern Cyprus taken in spring and autumn seasons was investigated. Gemlik Olive cultivar was used as the control group and 14 different local olive types constituted the experimental groups. Green cuttings taken from local olive types were initially treated with 4000 ppm indole-3-butyric (IBA) and they were then rooted in perlite medium under fogging unit in a plastic greenhouse. Following 100-days of rooting, rooting ratios, root lengths, number of roots and shooting ratios were determined. As expected, the best rooting ratio was obtained from Gemlik Olive cultivar (control group). Among the local types, the best rooting ratio (58.67%) was obtained from Çamlıköy with Type 8 olive type and the best rooting performance (45.87%) was achieved in cuttings rooted in autumn season.