In this study, the effect of different concentrations of smoke solution derived from sage (Salvia officinalis L.) on root and shoot growth of grass pea (Lathyrus sativus L.) was investigated in pots, in petri dishes (in vivo) and in vitro conditions. Smoke solution was obtained from hookah method and different concentrations (25%, 50%, 75%, 100%) were prepared by diluting the stock solution with distilled water and, distilled water was used as control. Solutions were used starting water of petri and perlite media and to prepare MS0 for in vitro condition. Nodal segments of grass pea seedlings as explants were cultured on MS0 medium in vitro. Plant nutrients, antioxidants, organic or inorganic chemicals, and plant growth regulators are commonly used for plant development both in vivo and in vitro. However, their use has risks in terms of economic costs as well as nature, environment and human health. Therefore, use of naturally derived chemicals in these applications has great advantages. Observations for in vivo conditions were determinate after 7 days from sowing and 15 days after in vitro culture. The longest root length (6.089 cm) was determined in 75% smoke solution of sage and, while the longest shoot length (3.026 cm) was obtained from 100% smoke solution of sage on petri media. In perlite media, the highest root and shoot length were observed in pure water (control). İn vitro conditions, although shoot formation was above 85% in all applications, root formation was under 33%. The shortest shoot length was obtained from smoke solutions of 100% (5.02 cm), the longest shoot length was obtained from 25% and 75% concentrations of smoke solution, respectively 8.35 and 8.94 cm.