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In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon Tissues
2022
Emine Yurteri | Mücahit Salih Can | Fatih Seyis | Haydar Kuplemez
Tea (Camellia sinensis) is the world's most popular beverage plant, as well as an important plantation crop with high commercial value. It has been maintained for centuries through conventional vegetative propagation. Tea clonal propagation in vitro has the advantage of producing a large number of elite plants. If an efficient in vitro regeneration technology is available, this technique could be exploited for selection of tea plants for desired trait. The selected plants could be later on multiplied through in vitro or ex vitro techniques. The study aimed to induced somatic embryogenesis from immature embryo explants to genetic variaton. Different concentrations of phenylboronic acid with benzyladenine and phenylboronic acid with kinetin were tested in MS medium with 30 g/L sucrose and 8 g/L agar. MS medium without any plant growth regulators was used as control group. Considering the embryo survival rate, 1.5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 kinetin produced highest result as 87.3% while lowest was in control group as 36.7%. The highest plant regeneration rate was found in 1,5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 kinetin and 1.5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 benzyladenine medium respectively as 58.3% and 55.6%. Kinetin treatment with increasing phenylboronic acid concentrations gave the best results in terms of somatic embryo survival rate. Also, kinetin treatment produced better results when compared to benzyladenine concentrations.
Afficher plus [+] Moins [-]Future and Prospect use of Pyrethrum (Chrysanthemum cinerariifolium) as Part of the Integrated Pest and Disease Management (IPDM) Tool in Turkey
2021
Flavien Shimira | Senem Uğur | Şamil Muhammet Özdemir | Yeşim Yalçın Mendi
Nowadays, immediate environmental friendly solutions such as the use of biopesticides and other methods to control and manage pests are well needed. They are imperative due to the continuous accumulation of toxic residues from synthetic insecticides into the environment, the contamination of global agro-ecosystem and resistance of certain insects and pathogens. The global renewed interest of botanical pesticides does not leave aside Turkey. Thus, many environmental reports pointed out contaminations in different regions of Turkey by chemical pesticide residues, like lambda-cyhalothrin in some conventional grapes farming in the Aegean region. The use of botanical pesticides like Pyrethrin extracted from Pyrethrum’s flowers (Chrysanthemum cinerariifolium) is part of sustainable agriculture goals to reduce the incidence of pests and diseases without any alteration to the natural balance. The purpose of this review is to analyse and identify the possibilities of Pyrethrum production in Turkey. Various research works around the world were compiled and some key informants were correlated to existing researches in Turkey. Subsequently, it was found that the Black-Sea region of Turkey has great potential in the growing and industrial production of Pyrethrum by its weather patterns (temperatures, pluviometry and humidity) and soil characteristics. The region has a similar climate with the East-African high-lands well-known for Pyrethrum production. Thus, the country has shown great technological advances and the capacity to produce vegetative and disease-free clones for other ornamental plants by using tissue culture techniques. Therefore, the multiplication of high-quality vegetative clones of Chrysanthemum cinerariifolium and their maintenance can sustain commercial and long term production of Pyrethrum in Turkey.
Afficher plus [+] Moins [-]Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines
2020
Tolga İzgü | Hülya İlbi | Yeşim Yalçın Mendi
Development of an efficient plant regeneration protocol is essential for vegetable crop advancement by biotechnological methods. In this study, regeneration protocols of four pepper lines of different pepper types were optimized. Different protocols for organogenesis were investigated in regeneration experiments. Optimum plant regeneration was obtained in different combinations of 6-Benzylaminopurine (BAP) and Indole-3-acetic acid (IAA) in organogenesis assays. In organogenesis experiment, the highest shoot development was determined as 80% from hypocotyl explant of Demre pepper in 4 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of Charleston pepper in 3 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of capia pepper in 5 mg L-1 BA+0.5 mg L-1 and 84% from hypocotyl explant of bell pepper in 5 mg L-1 BA+0.5 mg L-1 IAA. Afterward, shoots were rooted and whole plants were obtained.
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