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Grape Leaves as a Natural Antioxidant Source: Determination of Total Phenolic Compound Changes
2019
Zehra Babalık | Nilgün Göktürk Baydar
In this study, total phenolic compounds which possess health-promoting effects and provide basic nutrition were aimed to determine the alterations in different grapevine leaves. Total phenolic content was determined using Folin-Ciocalteu colorimetric method by using a spectrophotometer. At the result of the study, it was determined that contents of total phenolic in leaves collected from different grape cultivars were varied to cultivars and grape leaves had high phenolic compounds as berries. Therefore the grape leaves especially wine grapes such as Narince, Boğazkere ve Kalecik Karası may be used as an easily accessible source of natural antioxidants and food supplement at pharmaceutical and food industry.
Afficher plus [+] Moins [-]Preliminary Evaluation of Anti-Listerial Bacteriocin-like Peptide Produced by Enterococcus lactis PMD74 Isolated from Ezine Cheese
2019
Başar Uymaz Tezel
Enterococcus lactis PMD74 is a novel strain with a notably high antimicrobial activity. The present study evaluated the anti-listerial effect of a bacteriocin-like peptide (BLIP) produced by E. lactis PMD74 isolated from Ezine cheese (PDO). The strain was screened for its antimicrobial activity against 22 indicator strains using both agar spot and well diffusion methods. We observed that the neutralized cell-free supernatant (CFS) of E. lactis PMD74 exhibited varying levels of antimicrobial activity against both closely and distantly related pathogenic strains, with the highest activity displayed against Listeria monocytogenes strains. Although thermostable and resistant to lysozyme treatment, BLIP could be completely inactivated by trypsin, proteinase K, and α-chymotrypsin treatments. BLIP production starts in the early exponential growth phase of E. lactis PMD74 (3 h incubation, 400 AU mL–1) and reaches its maximal production (6400 AU mL–1) at the end of the exponential growth phase. Moreover, it is stable in the pH range of 2.0 to 7.0. The treatment of cultures of L. monocytogenes ATCC 7644 and Escherichia coli ATCC 26922 with sterilized CFS exhibited bactericidal and bacteriostatic effects, respectively. Furthermore, co-inoculation of L. monocytogenes ATCC 7644 and E. lactis PMD74 in skim milk led to complete loss of viability of L. monocytogenes ATCC 7644. These findings suggest that BLIP produced by E. lactis PMD74 could serve as a promising food preservative agent owing to its bactericidal and bacteriostatic properties.
Afficher plus [+] Moins [-]Degradation Kinetics of Anthocyanins in Shalgam Beverage
2019
Adnan Bozdoğan | Kurban Yaşar
This research was performed to elucidate the effects of temperature on the degradation kinetics of anthocyanins in shalgam beverage. Shalgam beverage was produced according to traditional production method. Then, it was kept at three different temperatures (65°C, 75°C, and 85°C) for 12 hours, and the relevant quantities of anthocyanins were determined thereafter. The research revealed that degradation of the anthocyanins was well described with a 1st-order reaction kinetics model and the R2 values varied in the range of 0.9059-0.9715. Activation energy of the reaction was determined to be 48537 Joule/mole. The half-lives of anthocyanins at 65°C and 75° C, and 85°C were found to be 138.63, 136.72, and 51.57, respectively. Compared the half-life periods at different temperatures, anthocyanins were found to be more resistant at 65°C and 75°C than at 85°C.
Afficher plus [+] Moins [-]Effects of Extracts of Feed Additives Including Rosemary (Rosmarinus officinalis) and Aloe Vera (Aloe barbadensis) on the Growth Performance and Feed Utility of Nile Tilapia (Oreochromis niloticus)
2019
Ebru Yılmaz | Deniz Çoban | Birsen Kırım | Mehmet Güler
This study was performed to determine the effects of the extracts of two alternative antimicrobial agents that do not harm the ecosystem (rosemary, Rosmarinus officinalis and aloe vera, Aloe barbadensis) when added to the feed of tilapia (Oreochromis niloticus) on the growth performance, proximate composition, and biometric characteristics. Rosemary extract was added to the experimental diet in ratios of 0.1%, 0.25%, and 0.5% while aloe vera extract was added to the experimental diet in ratios of 0.5%, 1%, and 2.5%. After 90 days of the feeding trial, no change was seen in the weight increase, specific growth rate, feed conversion ratio, condition factor, hepatosomatic index, or viscerosomatic index of the tilapia. The fact that proximate analyses did not differ between groups showed that the plant extracts did not adversely affect the health status of tilapia in the ratios used in this study. As a result, the addition of 0.1%, 0.25%, and 0.5% rosemary extract and the addition of 0.5%, 1%, and 2.5% aloe vera extract to tilapia feeds did not cause changes in the growth performance, biometric indexes, or chemical composition findings of the fish meat.
Afficher plus [+] Moins [-]Effects of Different Sowing Densities on Some Agronomic Characteristics of Camelina (Camelina sativa L.)
2019
Güngör Yılmaz | Şaziye Dökülen | Ahmet Kınay
This research was carried out to determine the appropriate sowing density in camelina in Tokat ecological conditions. The study was carried out using Ames 26680 camelina genotype in 2016 and 2017 for two years. In this study, 10, 20, 30 and 40 cm row spacings were used. Plant height (cm), number of branches per, number of capsules per plant, number of seed / capsule, thousand seed weight (g), seed yield (kg/da), oil ratio (%) and oil yield (kg/da) were investigated. According to the results, the seed yields of the two years were significantly different. The highest yields were obtained from 20 cm (132,0 kg/da) in 2016 and from 40 cm row spacing (130,6 kg/da) in 2017. As the average of two years, yields from 20, 30 and 40 cm row spacings did not differ significantly. As the average of two years, the oil rate was 36,2% and the oil yield was 43 kg/da. Thus, it is concluded that in fall sowing under Tokat conditions, camelina can be sown in 20 to 40 cm row spacing using 10 kg seed per hectare.
Afficher plus [+] Moins [-]Bazı Bitki Ekstraktlarının Tribolium confusum Duv. (Coleoptera:Tenebrionidae) ve Rhyzopertha dominica F. (Coleoptera: Bostrichidae) Üzerindeki Kontakt Toksisitesi
2019
Şeyda Şimşek | Mehtap Gürsoy | Seher Karaman Erkul
Bu çalışmada, Reseda lutea L. (Resedaceae), Salvia absconditiflora Greuter & Burdet. (Lamiaceae), Thymus leucostomus Hausskn. & Velen. (Lamiaceae), Chorispora purpurascens (Banks & Sol.) Eig (Brassicaceae), Isatis floribunda Boiss. ex Bornm. (Brassicaceae), Linum tenuifolium L. (Linaceae) bitkilerinden elde edilen methanol ekstraktlarının Tribolium confusum Duv. (Col.: Tenebrionidae) ve Rhyzopertha dominica F. (Col.: Bostrichidae) üzerindeki toksik etkileri laboratuvar koşullarında test edilmiştir. Bitkilerden elde edilen methanol ekstraktları zararlıların erginlerine micro-aplicator yardımıyla uygulanmıştır. Yapılan tek doz (%10 (w/v)) etki çalışması sonucunda T. confusum (%23,17) ve R. dominica (%18,35)’ya karşı en yüksek kontakt toksisiteyi C. purpurascens bitkisinden elde edilen ekstrakt göstermiştir.
Afficher plus [+] Moins [-]Land Banking System in Agriculture
2019
Uğur Başer | Mehmet Bozoglu
Land banking is one of the systems used in the management and planning of agricultural lands. The aim of the study, to examine the land banking system and the reasons for land banking in Turkey, land banking activities with the establishment stage and examine samples in the world. Land banking in the agricultural sector contributes such as improvement in the land market, guiding the land market for sustainable development and land use planning. In addition, the land banking system is shaped according to the needs of each country and there are four approaches to how the market will have a responsibility and intervene in the market. The prevalence of small-scale enterprises, the scattered and multi-piece of farm lands, which are one of the major problems of Turkish agriculture, hinders the efficient use of resources, hampers the use of technology and threatens food security. Therefore, integrating systems such as land banking into agriculture is thought to contribute to the solution of structural problems.
Afficher plus [+] Moins [-]The Use of Germination Indices for Salinity Tolerance Classification of Pepper Cultivars
2019
Gamze Kaya
The study aimed to evaluate the use of germination indices as a screening tool for salinity tolerance during germination and early seedling growth of pepper cultivars, and to distinguish the potential for genetic responses to salt tolerance. In the study, the seeds of seven pepper cultivars were germinated at increasing NaCl levels of 5, 10, 15 and 20 dS/m and distilled water as the control treatment for 14 days. Germination percentage (GP), mean germination time (MGT), germination index (GI), germination stress tolerance index (GSTI), seedling length (SL), seedling fresh weight (SFW) and vigor index (VI) were investigated. Results showed that germination percentage decreased with increasing NaCl levels while the highest germination percentage at 20 dS/m was 92% in BT Burdem with no significant reduction. Seedling growth of pepper cultivars was severely inhibited by increasing salinity stress. SFW was depressed depending on reduction in SL due to increasing NaCl. BT-Burli and BT İnce Sivri were the most tolerant cultivars to NaCl and they were used for genetic resources towards salinity. Seedling growth was much more sensitive to salinity than germination because of the highest percent reduction in seedling growth parameters. Among the parameters, GSTI gave the highest significant correlation coefficient with SL and SFW; indicating that it would be useful for estimating seedling growth. It was concluded that genotypic variation was observed among pepper cultivars for salinity tolerance and GSTI could be used for a predictor for salinity tolerance.
Afficher plus [+] Moins [-]Prevalence and PCR Sensitivity Comparison of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in Salads and Appetizers Consumed in Istanbul
2019
Zahide Bilgin | Gülay Merve Bayrakal | Emek Dümen | Gözde Ekici
This study was conducted to investigate incidence of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in 100 samples of salad and appetizers (50 salad and 50 appetizer samples) collected from retailers located various districts of Istanbul. Conventional microbiological methods and PCR procedures were used for Listeria monocytogenes and Staphylococcus aureus while only PCR procedures were used for the analysis of Toxoplasma gondii. Also PCR specifity and sensitivity for all the positive samples were calculated. According to the results, Listeria monocytogenes was detected in 9 samples (9%) and Staphylococcus aureus was detected in 36 (36%) samples while all the samples were negative for Toxoplasma gondii. PCR sensitivity results were quite specific and accurate for both Listeria monocytogenes and Staphylococcus aureus. It was concluded that salad and appetizers may be seriously threat consumers’ health microbiologically if they are processed under poor hygienic conditions.
Afficher plus [+] Moins [-]A Macroanatomic Study on Broncho-Pulmonary Segmentation in Hasak Sheep
2019
Zekeriya Özüdoğru | Hatice Özdemir
In this study, 10 Hasak sheep lungs from Konya Bahri Dağdaş International Agricultural Research Institute, Unit of Experimental Animals Unit were used. Corrosion casting technique were applied to the materials. The corrosion cured materials were stored in water for one night. It was then incubated at 37 ° C in a 20% KOH solution for 24 hours. Corrosion casting technique and latex injection materials were photographed and presented in the study. The lungs were found to be wrapped with pleura visceralis (pleura pulmonalis) in the cavum thoracis. It was determined that the right lung was consisted of lobe cranialis (pars cranialis and pars caudalis), lobus medius, lobus caudalis and lobus accessorius. The left lung was composed of lobe cranialis (pars cranialis and pars caudalis) and lobe caudal. In the bronchus that shaped these lobes, it was determined that tacheae gave bronchus lobaris cranialis dexter (bronchus trachealis) mean 48.53 mm before the bifurcatio trachea, and later on it was divided into bronchus pirincipalis dexter and sinister. In this study, anatomical features of broncho-pulmonary segmentation and similarities and differences with other species were revealed.
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