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Molecular Survey of the Toxoplasma gondii and Neospora caninum in brain tissue of aborted fetuses of Morkaraman sheep in Muş, Türkiye
2023
Davut Koca | Burçak Aslan Çelik | Özgür Yaşar Çelik | Adnan Ayan | Özlem Orunç Kılınç | Ali Osman Turgut | Özge Oktay Ayan
Toxoplasma gondii and Neospora caninum are obligate intracellular protozoan parasites that can affect different warm-blooded species worldwide. In this study, it was aimed to detect T. gondii and N. caninum using PCR method in brain tissues of aborted sheep fetuses. Brain specimens were collected from 50 Morkaraman sheep fetuses that had undergone abortion at various stages of pregnancy, within the lambing seasons of 2023 in Muş. Approximately 1 cm³ of brain tissue from the right cerebral hemisphere was excised and subsequently frozen at -20°C for DNA extraction. DNA extraction and PCR amplification were then performed. As a result of this study, 11 (22%) of 50 brain tissues were positive. All brain samples examined in this study were negative for Neospora caninum. Based on the results of this study, it is possible to say that T. gondii is an important abortion agent in sheep in this region. Although N. caninum was not detected in this study, larger scale studies are recommended. Moreover, this study provides important information to breeders and veterinarians in the field in the evaluation and management of abortion.
Afficher plus [+] Moins [-]Investigation of the Protective Role of Quercetin on Oxidative Stress and Endoplasmic Stress Pathway in 4-aminopyridine-induced Neuronal Damage
2023
Ahmet Taşkıran | Ayşe Topçu
Quercetin (QU) is a flavonoid found in different fruits and vegetables. Studies report that QU may have positive effects on neurological diseases. However, the effect of QU on 4-aminopyridine (4-AP)-induced neurodegeneration in neuronal cells is still not fully elucidated. In this study, the effects of QU on 4-AP-induced hippocampal neuron damage in vitro and the possible role of oxidative stress and endoplasmic reticulum stress in this effect were investigated. The study was carried out using the HT-22 hippocampal neuronal cell line. The effect of pre-treatment with QU on cell viability after 4-AP-induced neuronal damage was determined by the XTT test. Cells were evaluated histopathologically for apoptotic nuclear change (ANC) using DAPI staining. The effects of QU on oxidative stress (total oxidant state (TOS) and total antioxidant status (TAS)) occurring after neuronal damage were evaluated with colorimetric commercial kits and endoplasmic reticulum stress markers (activating transcription factor 4 (ATF-4) and C/EBP homologous protein). (CHOP) was measured with the ELISA kits. While the cell viability rate decreased in the cells treated with 4-AP, it was determined that pre-treatment with QU reversed this situation. In terms of histopathology, treatment with 4-AP increased the number of ANC, while QU pre-treatment reduced it. In addition, in terms of biochemical evaluations, TOS, ATF-4, and CHOP increased in neuronal cells after 4-AP, and QU was determined to suppress this increase. In addition, QU normalized the decreased TAS levels following the 4-AP application. As a result, in the HT-22 cell line, it was found that QU treatment had a neuroprotective effect by suppressing oxidative stress and endoplasmic reticulum stress in 4-AP-induced neuronal damage.
Afficher plus [+] Moins [-]Phylogenetic Analysis and Lipoxygenase (LOX) Gene Family Variation in The Pistachio
2023
Elmira Ziya Motalebipour | Akbar Pirestani
Lipoxygenases (LOX) gene family is a type of nonheme iron-containing dioxygenases, which has a very important aspect in plant development and fruit quality. LOX gene, which is responsible for lipid oxidation, the main role for the biosynthesis pathway of unsaturated fatty acids. Although some studies have investigated the LOX gene family in several species including arabidopsis, soybean, peanut and apple, there is no information from Pistachio; and the phylogeny of this gene family in the Pistacia genus is still not determined. In this study, Arabidopsis thaliana LOX1 gene (NCBI Reference Sequence: NM_104376.3) was selected and used as a query sequence for performing a BLASTN search. Among all sequence query which was found by NCBI platform, 9 sequences were selected for further analysis. Phylogenetic tree of full-length LOX gene sequences from the Pistacia genus was constructed using the Maximum Likelihood method with MEGA software. By using phylogenic analysis, we identified variations in gene structure and revealed the phylogenetic evolutionary relationship of this gene family. Additionally, this may serve as a reference value for assessing the genetic relationships among various LOX genes in Pistacia genus species. This variations provides us the possibility the design the primer to achieve us to find the exact LOX gene in Pistacia genus and future research on the evolutionary history and transgenic research on LOX genes.
Afficher plus [+] Moins [-]Effect of Seed Rates and Sowing Dates on Productivity of Wheat (Triticum aestivum L.)
2023
Pawan Gyawali | Pankaj Gyawali | Bishnu Bilas Adhikari | Dharma Raj Katuwal
A field experiment was conducted at the Agronomy farm of Nepal Agricultural Research Council (NARC), Khumaltar, Lalitpur to evaluate the effect of sowing dates and seed rates on yield and yield attributes of wheat. The experiment was laid in a split-plot design with three replications treated with 4 sowing dates as the main plot factor (12th Nov, 27th Nov, 12th Dec, and 27th Dec) and 4 seed rates as subplot factor (100 kg ha-1, 120 kg ha-1, 140 kg ha-1 and 160 kg ha-1). Results revealed that the leaf area index was significantly affected by sowing dates and was comparatively superior in 2nd sowing date (27th Nov) wheat. Similarly, in the case of seed rates, the leaf area index was influenced significantly and was recorded to be increasing with an increase in seed rates. Phenological parameters like days to 50% heading, flowering, and maturity were observed maximum (116, 123, and 179 days, respectively) in early sown wheat and reduced with the subsequent delay in sowing. Maximum values of yield and yield attributes like effective tillers per meter square (635.6), spike length (9.56 cm), grains per spike (41.49), grain yield (7.59 Mt ha-1), and straw yield (9.58 Mt ha-1) were observed in the wheat sown in 2nd date (on 27th Nov) which differed significantly to wheat sown on other dates. Seed rates had no significant influence on grain yield and yield attributes. Thousand-grain weight was found maximum (46.26 g) in early sown wheat (on 12th Nov sown wheat, reduced with the subsequent delay in sowing, and the harvest index was observed as maximum (0.51) under December 27 sown wheat. Though the yield and its attributes were not influenced significantly by seed rate, the maximum yield (6.18 Mt ha-1) was observed in wheat sown at the rate of 120 kg ha-1. Considering seed yield and its parameters, 2nd date of sowing wheat (27th Nov) and seed rate of 100 kg ha-1 could be the best option to uplift the productivity of wheat in rainfed lowland conditions of Lalitpur, Nepal.
Afficher plus [+] Moins [-]Ecological Advancements and Developments of Agroforestry
2023
Shitosri Mondal | Prodipto Bishnu Angon | Arpita Rani Roy
Agroforestry is a conventional method of land use that could help to address agricultural environmental issues. In order to take advantage of the ensuing ecological and economic interactions, agroforestry is the technique of consciously integrating woody vegetation (trees or shrubs) with crop and/or animal systems. According to recent studies, the global agri-food industry may reach more sustainable methods of producing food and fiber by adopting agroforestry techniques and principles more widely. This would benefit farmers economically and would benefit society as a whole in terms of the environment. Agroforestry promotes eco-intensification based on resource efficiency and offers a wide range of provisioning, regulating, cultural, supporting ecosystem services, and environmental advantages. In this review, we discussed agroforestry with its advantages and developments.
Afficher plus [+] Moins [-]An Example of an Application Project on Contemporary Office Design
2023
Hatice Çınar
This study aims to define the concept of office spaces and their requirements, as well as the contemporary interior design approaches. It will also cover the interior design and application process for an architecture office in Konya. The company, originally named Çınar Architecture and active in Konya between 1985 and 1990, has been operating as Kerimler Planning Construction Consultancy Company in Konya since 1995. The company requested an interior architecture project for their new location. In the current environment, the office is starting to become outdated and is unable to offer users enough physical amenities. The main goal of the new office’s interior design was to create areas where employers and employees could collaborate in a welcoming and adaptable setting without facing any kind of discrimination. In Konya Meram Pirebi District, the Menekşe Apartment’s ground floor, which is currently a street-level store, is the site of the project, which occupies a total of 280 m2 and consists of a ground + basement. The office occupies 145 square meters on the ground floor of a recently constructed building, with glass extending to the open floor on the south, north, and west facades. The room has a very large opening because the columns are concealed by the walls, making the two stories easily visible from the entrance. The office, which occupies a 135 m2 basement floor, is connected to the elevator and staircase at the back. Natural light enters the basement through the gallery area that is attached to the staircase. Within the parameters of the study, two- and three-dimensional architectural design tools, including phases of the interior design process, were employed in conjunction with a qualitative research method based on inspection and observation. The architectural office put it into practice about half a year after the design stage. The outcome was the creation of a dynamic, modern office analysis that values teamwork and permits the emergence of unique and creative ideas throughout the project.
Afficher plus [+] Moins [-]A Comprehensive Study on the Competitiveness of Governing Structures of Bulgarian Farming
2023
Bilal Kargı | Hrabrin Bachev
The farm is an abstract category in Economic theory for describing agents managing farming activity, while the real governing structures are farms of different juridical types – physical persons, sole traders, cooperatives, ago=companies, etc. Most of the time, farm’s competitiveness is inadequately assessed through technical and accountancy efficiency, factors’ productivity, profitability, market shares, etc. because critical governance aspects are ignored. This article incorporates the interdisciplinary New Institutional Economics assumptions and principles and tries to give new insights on the real competitiveness of economic organizations in modern agriculture. It suggests a holistic framework for assessing farm’ competitiveness taking into account economic, financial, and governance efficiency, and evaluates absolute and comparative competitiveness of governing structures of Bulgarian farming. The novel assessment system includes four pillars, four criteria, 17 particular, and 5 integral indicators. The first-in-kind evaluation, based on survey data, found that the competitiveness of Bulgarian farms is good. The competitiveness of cooperatives is highest, followed by corporations and associations, sole traders, and physical persons. Critical for competitive positions of farms are: low productivity, income, financial security, and adaptability to natural environment, where public support and farms’ management strategies should be directed. Large shares of the country’s farms have low competitiveness, and if measures are not taken to improve management, restructuring, state support, etc., many farms will cease to exist in the near future. In some cases, other characteristics of governing structures like size, specialization, market orientation, and ecological location, are critical for determining competitiveness level. The suggested and successfully tested framework for assessing the competitiveness of farms should be further improved and applied more widely and periodically in the country and internationally. The precision and representativeness of the information used should also be improved by increasing the number of surveyed farms and their important characteristics. The later requires close cooperation with producer organizations, national agricultural advisory service, and other interested parties as well as extending and improving the system for collecting agro-statistical information in the country and the EU.
Afficher plus [+] Moins [-]Influence of Foliar Application of Boron on the Growth, Yield and Quality of Sesame (cv. BARI Til-4)
2023
Apu Dey | Mahfuza Begum | Md. Sojib Kabiraj | Md. Harun Rashid | Swapan Kumar Paul
Foliar supplementation may be useful for boron to a crop when its demands are higher compared to the supply from soil. Boron is an important micronutrient which had a substantial impact on oil content, seed yield, and the components of the sesame yield. A field investigation was implemented to determine the impact of foliar application of boron on seed yield, growth and oil content of sesame cv. BARI Til-4. The investigation included four concentrations of boron viz. 0, 25, 50, and 75 ppm and three frequencies of boron application viz. one time at 30 days after sowing (DAS), two times at 30 and 50 DAS, and three times at 30, 50, and 70 DAS. The experiment was conducted following randomized complete block design, which was replicated thrice. At the vegetative stage, the highest plant height (107.3 cm), branches/plant (5.0) and shoot dry weight (45.20 g/plant) resulted in 75 ppm boron spray at 30, 50 and 70 DAS. However, the highest root dry weight (5.80 g/plant) was recorded in 75 ppm with one application of boron at 30 DAS. The plants with the highest plant height (112.1 cm), branches/plant (5.13), pods/plant (44.13), seeds/pod (54.33), seed yield (609.0 kg/ha), harvest index (30.65%), and oil content (42.33%) were also observed with the combination of 75 ppm boron spray with thrice application at 30, 50 and 70 DAS. The lowest seed yield (368.7 kg/ha) resulted in without boron application. Therefore, it can be inferred that the most efficient method for increasing the sesame seed yield and oil content is thrice (30, 50, and 70 DAS) foliar spraying of 75 ppm boron.
Afficher plus [+] Moins [-]Orange Peel and Cauliflower Residues Supplementation Induce Morphological and Physiological Tolerance in Common Bean under Drought Stress
2023
İlkay Yavaş | Elif Sude Çiçek
Drought is one of the most harmful abiotic stresses affecting the development and yield of the common bean (Phaseolus vulgaris L.). The current climate change and the resulting increased drought will worsen the negative impact of water stress on the plant. The powder of orange peel and cauliflower waste were added as soil supplementation at rates of 7 and 15 g/pot to pots of Phaseolus vulgaris L. under different drought conditions. The growth and physiological analysis were estimated after flowering period of common bean. In the pots where drought will be applied, irrigation was stopped for 2, 4, 6, 8 and 10 days during the flowering period and irrigation was performed again after water stress application. To measure moisture percentage of pots, first dry pots were weighed and after irrigation, their moisture variation in terms of percent was measured during stress from 2 to 10 days. The highest plant height was obtained from control. Leaf area decreased significantly despite the application of different powder, especially after 4 days of drought conditions. The highest root fresh and dry weight, raw ash were observed under control with the application of 7.5 g orange peel powder. Shoot dry weight decreased as the number of days exposed to drought increased, and the application of 15 g orange peel and cauliflower powder gave the highest results compared to control conditions. The highest dry matter was obtained from the application of 7.5 g and 15 cauliflower powder in the absence of drought. It has been revealed that as the duration of exposure to drought increases, the value decreases and plant powders are effective in increasing this value. Chlorophyll a, chlorophyll b and total chlorophyll values decreased significantly with drought, and the highest value was obtained from control conditions, followed by 15 cauliflower powder applications.
Afficher plus [+] Moins [-]Spatial Evaluation of Land and Soil Properties with Geography Information Systems (GIS): The Case Study from Meriç District of Thrace Region in Türkiye
2023
Muhammed Cüneyt Bağdatlı
This research was carried out within the scope of spatial evaluation of the land and soil properties of Edirne-Meriç district, located in the Thrace region (Türkiye) by using GIS. Arc GIS 10.3.1 software was used in the classification of soil and land features. Digital soil maps (1/25.000 scale) were used to determine soil and land properties. Alos Palsar (12.5m) satellite images were used to determine land elevation and aspect distributions. As a result of the research, it was seen that the soil class with the largest area in terms of large soil groups in Meriç district is lime-free brown forest soils (261.2 km2). III. It was determined that class lands (153.7 km2) cover the largest area. In general, it was observed that the soil depth was greater than 150 cm (261.9 km2). It was determined that 23.3 km2 of the Meriç district lands were exposed to severe water erosion. It was determined that the study area consists of lands with a steep slope of 12-20% (126.7 km2). It has been observed that the height distribution of Meriç district lands varies between 4.7-120.5 m. It was defined that the majority of the lands were in the southwestern direction group. It is thought that the soil and land information database created as a result of the research will make significant contributions to researchers and the public, institutions and organizations.
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