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Assessment of Inorganic Phosphorus Fractions of Fruit and Vegetable Dumpsite Soils as a Strategy for Mitigating Eutrophication Hazard Texte intégral
2025
Rejoice Ibrahim Solomon | Binamem Simon | Aishatu Mala Musa | Yusuf Shehu
Assessment of Inorganic Phosphorus Fractions of Fruit and Vegetable Dumpsite Soils as a Strategy for Mitigating Eutrophication Hazard Texte intégral
2025
Rejoice Ibrahim Solomon | Binamem Simon | Aishatu Mala Musa | Yusuf Shehu
Phosphorus is essential for plant growth, but mishandling phosphorus-rich waste can harm the environment. This study assessed inorganic phosphorus from fruit and vegetable dumpsite soils and its relation to soil properties. Fifteen (15) soil samples were collected and homogenized into five (5) representative samples each from the dumpsites at depths of 0-15cm, 15-30cm, and 30-60cm and were analyzed for calcium-bound phosphorus (Ca-P), reductant soluble phosphorus (RS-P), Fe-bound Phosphorus (Fe-P), aluminium-bound phosphorus (Al-P), and Soluble/loosely bound P. The data obtained was analyzed using the student’s t-test to compare the different fractions in fruit and vegetable dumpsite soils. The results showed no significant differences between fruit and vegetable dumpsite soils in all the inorganic P fractions. However, vegetable dumpsite soils recorded higher soluble P, Al-P, Fe-P, RS-P, and Ca-P levels of 11.65, 36.29, 41.95, 40.75, and 41.35 mg/kg, respectively. These values were not significantly different from 9.75, 34.01, 39.28, 37.11 and 41.03 mg/kg recorded by fruit dumpsite soil for soluble P, Al-P, Fe-P, RS-P and Ca-P respectively. Results also revealed that reductant soluble P, Calcium bound P, and Na+ with an R2 value of 0.88 had a combined significant influence on the concentration of soluble P. However, the concentration of the inorganic P fractions for vegetable dumpsite soil is in the order; Fe-P> Ca-P>RS-P>Al-P>soluble P, while that of fruit dumpsite soil is in the order; Ca-P>Fe-P> RS-P>Al-P>soluble P. Soluble phosphorus significantly impacts eutrophication due to its rapid release into water. However, low levels of soluble P in fruit and vegetable dumpsite soils are not a major concern. In vegetable dumpsite soil, Fe-P would pose a greater threat during anoxic events while, in fruit dumpsite soil, Ca-P would contribute more to eutrophication due to its high concentration in this soil, especially in alkaline conditions.
Afficher plus [+] Moins [-]In vitro antifungal activity of chitosan on Bipolaris oryzae, rice pathogen | Actividad antifúngica in vitro de quitosanos sobre Bipolaris oryzae, patógeno del arroz Texte intégral
2016
Rodríguez Pedroso, Aida Tania | Plascencia Jatomea, Maribel | Bautista Baños, Silvia | Cortéz Rocha, Mario Onofre | Ramírez Arrebato, Miguel Ángel
The aim of this study was to determine the effect and Media Inhibitory Concentration (MIC50), of chitosans of different molecular masses on mycelial growth, hyphal diameter, structural changes, spore germination and fungal biomass production B. oryzae. The results indicated that the average molecular mass chitosan showed higher inhibitory effect (p≤0.05); however, the low molecular weight chitosan affected more hyphal diameter at the highest concentration used (4000 mg/l) Observations of hyphae through the scanning electron microscopy (SEM) showed that the fungus treated with chitosan at the concentration of 3000 mg/l achieved a high spore production. The average molecular mass chitosan had the lowest MIC value was 2182.9 mg/l. The germination of spores treated with CMI50 was affected and biomass production of the fungus. | En el presente trabajo se evaluó el efecto y la Concentración Media Inhibitoria (CMI50) de quitosanos de diferentes masas moleculares en el crecimiento micelial, el diámetro de las hifas, los cambios estructura-les, la germinación de esporas y la producción de biomasa del hongo Bipolaris oryzae. Los resultados mostraron que el quitosano de masa molecular media presentó mayor efecto inhibitorio (P ≤ 0.05); sin embargo, el quitosano de masa molecular baja afectó más el diámetro de las hifas a la mayor concentra-ción utilizada (4000 mg/l). Las observaciones de las hifas realizadas por Microscopía Electrónica de Ba-rrido (MEB) mostraron que el hongo tratado con quitosano a la concentración de 3000 mg/l presentó una alta producción de esporas. El quitosano de masa molecular media presentó el valor más bajo (2182.9 mg/l). La germinación de las esporas tratadas con CMI50 y la producción de biomasa del hongo fueron afectadas por el quitosano.
Afficher plus [+] Moins [-]Effects of Different Doses of Lactobacillus brevis Addition to Oat (Avena sativa L.) Silage on Physical and Chemical Properties, Aerobic Stability, in Vitro Organic Matter Digestibility and Energy Value of Silage Texte intégral
2025
Hayrettin Çayıroğlu
Effects of Different Doses of Lactobacillus brevis Addition to Oat (Avena sativa L.) Silage on Physical and Chemical Properties, Aerobic Stability, in Vitro Organic Matter Digestibility and Energy Value of Silage Texte intégral
2025
Hayrettin Çayıroğlu
This study aimed to determine the effects of the addition of different doses of Lactobacillus brevis to oat silage on the physical and chemical properties, aerobic stability, in vitro organic matter digestibility, and energy value of the silage. In the study, the treatment groups were oat control (OC), 1x106 (Lb6), 1x108 (Lb8) and 1x109 cfu/kg dry matter (Lb9) L. brevis inoculated oats. Inoculation of L. brevis into oat silage increased the number of lactic acid bacteria and decreased the pH of the silage at the time of opening. This inoculation decreased the organic matter, acid detergent fiber and neutral detergent fiber contents of the silages at opening compared to those of OC, while increasing the dry matter and crude protein contents. The L. brevis inoculation into oat silage decreased the pH values and yeast counts on the fifth day after opening the silages compared to OC, without affecting the amount of CO2 production and mold count. The inoculation did not affect the in vitro organic matter digestibility and metabolizable energy value of silage; but increased the net energy lactation value compared to OC. When the L. brevis doses are evaluated independently, it can be said that 1*108 and 1*109 cfu/kg DM doses contributed more effectively to oat silage, so any of these doses can be preferred.
Afficher plus [+] Moins [-]Zmiany w produkcji, handlu zagranicznym i zużyciu krajowym ziemniaków w Polsce w latach 2001-2019 Texte intégral
Stańko, Stanisław | Mikuła, Aneta
The subject of the analysis was potato production, domestic consumption and foreign trade turnover (export and import) in Poland in 2001-2019. Based on the results of the analyzes, a medium-term projection (for 2025) was defined, covering production, directions of domestic use and foreign trade turnover of potatoes and their products. The analyzed data came from the Central Statistical Office and Institute of Agricultural and Food Economics- National Research Institute. Potato production in Poland in 2001-2019 was characterized by a downward trend. The slower drop in the harvest than in the area was due to the improvement in yield. There were various trends in domestic consumption: a decrease in total potato consumption, in the export of unprocessed potatoes, in processing in distilling and losses in storage, and an increase in food processing and other industrial processing. In foreign trade, an improvement in the positive balance of trade in potato products and a deepening of the negative trade balance in potatoes was observed. The development projection for 2025 shows that the production of potatoes in Poland may amount to 7,250,000- 7,540,000 thousand tonnes, and the demand – 7430 7570 thousand tonnes. The necessary import of fresh potatoes can amount to 30-180 thousand tonnes annually. / Przedmiotem analizy była produkcja ziemniaków, zużycie krajowe i obroty handlu zagranicznego (eksport i import) w Polsce w latach 2001-2019. W oparciu o wyniki analiz określono średniookresową projekcję (na 2025 r.) obejmującą produkcję, kierunki wykorzystania w kraju i obroty handlu zagranicznego ziemniakami i ich przetworami. Analizowane dane pochodziły z GUS oraz IERiGŻ-PIB. Produkcja ziemniaków w Polsce w latach 2001-2019 charakteryzowała się tendencją spadkową. Wolniejszy spadek zbiorów niż powierzchni wynikał z poprawy plonowania. W zużyciu krajowym występowały różnokierunkowe tendencje: spadkowa w spożyciu ziemniaków ogółem, w eksporcie ziemniaków nieprzetworzonych, w przetwórstwie w gorzelnictwie i w stratach w przechowalnictwie oraz wzrostowa w przetwórstwie spożywczym i pozostałym przemysłowym. W handlu zagranicznym zaobserwowano poprawę dodatniego salda handlu przetworami z ziemniaków oraz pogłębianie się ujemnego salda handlu ziemniakami. Z projekcji rozwoju na 2025 r. wynika, że produkcja ziemniaków w Polsce może wynosić 7250- 7540 tys. t, a zapotrzebowanie - 7430-7570 tys. t. Import niezbędny ziemniaków świeżych może wynosić rocznie 30-180 tys. t.
Afficher plus [+] Moins [-]In silico analyses of miRNAs that Target Odorant Binding and Chemosensory Proteins in Bemisia tabaci Texte intégral
2025
Mehtap Bal | Sevgi Maraklı
The whitefly, Bemisia tabaci, damages various crops by releasing honeydew and spreading. Although farmers and pest control experts primarily rely on insecticides to manage whiteflies, the notable issue is their tendency to develop resistance to major insecticide categories, posing a significant challenge. This result has led to the improvement of new drugs or insecticide mixtures. In addition, some plant-based studies have been conducted to control whiteflies, and RNA interference (RNAi) technology has been used in recent years. This study aimed to identify the relationships between tobacco, cotton, tomato, and linen miRNAs and odorant-binding protein (OBP) and chemosensory protein (CSP) genes in whiteflies by using in silico approaches. We determined that 115 miRNAs belonging to these plants targeted 13 CSP and 8 OBP genes of B. tabaci. Obtaining findings are important to reduce dependency on chemicals and pesticides in pest management.
Afficher plus [+] Moins [-]Boosting the Productivity of Bread Wheat (Triticum aestivum L.) Varieties through Optimal Seed Rates and Appropriate Systems for Irrigation Production System of Northwestern Ethiopia Texte intégral
2025
Zelalem Yadelew
Boosting the Productivity of Bread Wheat (Triticum aestivum L.) Varieties through Optimal Seed Rates and Appropriate Systems for Irrigation Production System of Northwestern Ethiopia Texte intégral
2025
Zelalem Yadelew
The availability of high-yielding varieties adapted to diverse agro-ecologies and production systems, preferred by farmers and consumers, is the key factor limiting productivity. Farmers access seeds of different quality levels from various seed systems. Studies on seed systems and rates in relation to yield and yield traits of bread wheat varieties under irrigation are limited. Therefore, an experiment was conducted in 2021 in Northwestern Ethiopia to improve the productivity of bread wheat (Triticum aestivum L.) varieties through suitable seed rates and systems under irrigation. The experiment was conducted using a randomized complete block design in a factorial arrangement of two varieties (Kakaba and Ogolcho), three seed systems (formal, intermediate and informal), and three seed rates (125, 150, and 175 kg ha-1) in three replications. SAS software was used for analysis. The results showed that the seed system and variety interaction had a significant effect (P < 0.05) on productive tiller numbers, days to 50% heading, kernel numbers per spike, and plant height. Additionally, the productive tiller numbers and days to 50% heading were significantly (P < 0.05) influenced by the interaction of variety with seed rate. However, the main effects of seed system, seed rate, and variety alone had a significant (P < 0.05) impact on physiological maturity, 1000-seed weight, kernel length, grain yield, biomass yield, and harvest index. The highest grain yields were obtained from the following main factors: seed system (intermediate (4.52 t ha-1); seed rate (150 kg ha-1 (4.71 t ha-1)); and variety (Kakaba (4.47 t ha-1)), which exceeded the average yield in irrigation (4.4 t ha-1). It is necessary to conduct experiments involving a greater number of seed rates and varieties over multiple cropping seasons and locations and sowing dates to strengthen the results.
Afficher plus [+] Moins [-]Evaluación in vitro de la actividad inhibitoria de extractos vegetales sobre aislados de Colletotrichum spp Texte intégral
2011
Rojas Sierra2 Johanna | Pérez Palencia Katy | Pérez Cordero Alexander | Chamorro Anaya Leonardo
Se evaluó la actividad inhibitoria in vitro de extractos de hojas de Melissa officinalis, Origanum vulgare,Jatropha gossypilia, Eucalyptus sp., Melia azederach y Mascagnia concinna sobre aislados de hongo delgénero Colletotrichum, causante de la enfermedad antracnosis en el cultivo del ñame (Dioscorea alata, D.rotundata) en el departamento de Sucre, Colombia. Para la obtención de extractos etanólicos de hojasde M. officinalis y O. vulgare, se utilizó el método de percolación y para las otras especies vegetales, elmétodo de Soxhlet. Una vez preparados los extractos de hojas, se evaluó su actividad inhibitoria sobreseis aislados de hongo del género Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 yBVC 507). Para la prueba inhibitoria se utilizó el método de siembra directa sobre la superficie del mediopapa-dextrosa-agar. Sobre las diferentes cepas se adicionaron 250 μl de cada extracto por separado. Seutilizó un control positivo con nistatina (4 mg/ml) y un testigo absoluto sin ningún tipo de tratamiento.La prueba se evaluó midiendo el crecimiento radial de cada cepa con los diferentes tratamientos a 96y 168 h. Se utilizó un diseño de bloques completamente al azar con tres repeticiones, para probardiferencias significativas entre tratamientos, cepas (aislados) y tiempo de inhibición. Los resultadosexpresados en porcentaje de inhibición, mostraron una alta actividad de los extractos obtenidos de lashojas de las plantas de M. azederach y M. concinna, sobre los hongos evaluados y su acción fue similaral control positivo
Afficher plus [+] Moins [-]Evaluación in vitro de la actividad inhibitoria de extractos vegetales sobre aislados de Colletotrichum spp Texte intégral
2011
Pérez Cordero, Alexander(Universidad de Sucre Facultad de Ciencias Agropecuarias Grupo Bioprospección Agropecuaria) | Rojas Sierra, Johanna(Universidad de Sucre Facultad de Educación y Ciencias Grupo Bioprospección Agropecuaria) | Chamorro Anaya, Leonardo(Universidad de Sucre) | Pérez Palencia, Katy(Universidad de Sucre)
Se evaluó la actividad inhibitoria in vitro de extractos de hojas de Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp., Melia azederach y Mascagnia concinna sobre aislados de hongo del género Colletotrichum, causante de la enfermedad antracnosis en el cultivo del ñame (Dioscorea alata, D. rotundata) en el departamento de Sucre, Colombia. Para la obtención de extractos etanólicos de hojas de M. officinalis y O. vulgare, se utilizó el método de percolación y para las otras especies vegetales, el método de Soxhlet. Una vez preparados los extractos de hojas, se evaluó su actividad inhibitoria sobre seis aislados de hongo del género Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 y BVC 507). Para la prueba inhibitoria se utilizó el método de siembra directa sobre la superficie del medio papa-dextrosa-agar. Sobre las diferentes cepas se adicionaron 250 µl de cada extracto por separado. Se utilizó un control positivo con nistatina (4 mg/ml) y un testigo absoluto sin ningún tipo de tratamiento. La prueba se evaluó midiendo el crecimiento radial de cada cepa con los diferentes tratamientos a 96 y 168 h. Se utilizó un diseño de bloques completamente al azar con tres repeticiones, para probar diferencias significativas entre tratamientos, cepas (aislados) y tiempo de inhibición. Los resultados expresados en porcentaje de inhibición, mostraron una alta actividad de los extractos obtenidos de las hojas de las plantas de M. azederach y M. concinna, sobre los hongos evaluados y su acción fue similar al control positivo. | The objective of this study was to evaluate in vitro inhibitory activity of extracts from leaves of Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp. Melia azederach and Mascagnia concinna on isolated fungus of the genus Colletotrichum, which causes anthracnose disease in the crop of yam (Dioscorea alata, D. rotundata) in the department of Sucre, Colombia. To obtain the ethanol extract from leaves of M. officinalis and O. vulgare methods of percolation were used and for the rest of plant species, the Soxhlet method was performed. Once leaf extracts were prepared, evaluation for inhibitory activity on six isolated fungus of the genus Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 and BVC 507) was carried out. Inhibitory to the test method was used for direct seeding on the surface of potato-dextrose agar. On the different strains were added 250 µl of each extract separately. We used a positive control with nystatin (4 mg/ml) and an absolute control without any treatment. The test was evaluated by measuring the radial growth of each strain with different treatments at 96 and 168 h. A randomized complete block with three replications, to test significant differences between treatments, strains and inhibit time was used. The results expressed as percentage of inhibition, showed high activity of extracts obtained from leaves of plants of M. azederach and M. concinna, the fungi evaluated and its action was similar to the positive control.
Afficher plus [+] Moins [-]Investigation of Antioxidant Properties of Spartium junceum L.: Effect of Plant Parts and Storage Conditions Texte intégral
2025
Muhammed Mehdi Üremiş | Ergül Belge Kurutaş
Spartium junceum L. is a plant traditionally used for different medicinal purposes. While limited research data explicates its antioxidizing ability, interest in this plant is induced mainly due to its possible role, especially against stress-causing oxidative effects. The objectives of this study were to compare antioxidant activity in flowers and leaves of Spartium junceum L., as well as time under different storage conditions implemented for antioxidative mechanisms. Spartium junceum L. plants were obtained from the Kahramanmaras, Turkey region; subsequently, the flowering and leaf parts of the plant were separated and analyzed. Plant homogenates were prepared, and the activities of SOD and CAT enzymes, as well as MDA levels, were determined using spectrophotometric methods. Enzyme activity upon storage at +4°C, -20°C, and -70° temperature enzyme samples were carried out separately and operated for less than one month in our laboratory. Flowers exhibited higher SOD and CAT activities than leaves. Flowers also showed higher levels of MDA. It may be due to the structural and biochemical differences, where flowers experience extra oxidative stress. The optimal enzyme retention under storage conditions was at -70°C, and a decrease in temperature increased the stability of this biocatalyst. In contrast, MDA levels increased at low temperatures at total capacity. The antioxidant properties of the flower extract had stronger antioxidant potential than those of the leaf part, which also means that chemically active substances show much higher concentrations in this plant section. Storage temperature significantly affects the stability of enzymes, and it was stated that low temperatures mainly maintain antioxidant activity. The results obtained from this study recommend Spartium junceum L. as a valuable antioxidant food resource.
Afficher plus [+] Moins [-]Trabzon Hurma Püresinin Reolojik Davranışının Modellenmesi Texte intégral
2025
Sevim Gürdaş Mazlum | Dilan Lodos
Bu çalışmada Trabzon hurması (Diospyros kaki L.) püresinin akış davranışını en iyi tanımlayan reolojik modelin belirlenmesi amacıyla pürenin reolojik özellikleri çeşitli pH’larda (4,0, 5,5 ve 7,0), konsantrasyonlarda (%15, %17,5 ve %20), sıcaklıklarda (25, 50 ve 75°C) ve kayma hızında (8,4–28 s−1) araştırılmıştır. Deneysel veriler Power Law, Herschel-Bulkley, Casson ve Mizhari-Berk modellerine uygulanmıştır. Reolojik modellerin akış davranışını açıklamadaki uygunluğunu doğrulamak için belirleme katsayısı (R2), hata kareleri ortalamasının kare kökü (RMSE) ve indirgenmiş ki- kare (ꭓ2) olmak üzere üç istatistiksel ölçüt kullanılmıştır. Herschel-Bulkley ve Mizhari-Berk, hurma püresinin tüm işlem koşullarında deneysel verilere en iyi uyum sağlayan modeller olduğu görülmüştür. Ancak Mizhari-Berk modeli R2 ≥ 0.983, RMSE ≤ 0.0683 ve ꭓ2 ≤ 0.0160 istatistiksel parametre değerleriyle hurma püresinin akış davranışını en iyi tanımlayan model olmuştur. Trabzon hurması püresi psödoplastik (kayma incelmesi) ve Newtonyen olmayan bir davranış (n<1) sergilemiştir.
Afficher plus [+] Moins [-]Sulama Durumu Tahmini için Makine Öğrenimi Algoritmalarının Karşılaştırmalı Analizi Texte intégral
2025
Betül Demir | Yeşim Dokuz | Burak Şen
Geleneksel yöntemlere kıyasla, makine öğrenimi ile desteklenen sistemlerin, daha hassas sulama kararları verebildiği tespit edilmiştir. Bu çalışmada, veri bilimi alanında sıkça kullanılan veri paylaşım platformu olan Kaggle’dan faydalanılmıştır. Sulama durumu tahmini için “Weather Data” veri kümesi kullanılmıştır, Veri kümesinde eksik ve aykırı veriler düzeltilmiş, bağımlı (sulama durumu) ve bağımsız (hava sıcaklığı, nemi, toprak nem değeri, yağış durumu) parametreler elde edilmiştir. Güneydoğu Anadolu Bölgesi’ndeki illere (Adıyaman, Batman, Diyarbakır, Gaziantep, Kilis, Mardin, Siirt, Şanlıurfa ve Şırnak) odaklanılarak farklı koşullarda algoritmaların doğrulukları test edilmiştir. Her bir il için ayrı ayrı yapılan analizlerde, makine öğrenimi algoritmalarından Karar Ağaçları, Destek Vektör Makineleri, Rastgele Orman, Naive Bayes, Gradyan Artırma, Lojistik Regresyon, K-En Yakın Komşu ve Yapay Sinir Ağı modelleri kullanılarak sulama durumu tahmini gerçekleştirilmiştir. Veri kümesi üzerinde yapılan tahminler sonucunda algoritmalar, doğruluk (accuracy) metriği kullanılarak karşılaştırılmış ve en etkili algoritmaların Rastgele Orman (%95) ,Karar Ağacı (%97), Gradyan Artırma (%93) ve Yapay Sinir Ağı (%98) modeli ile tüm şehirlerde %90’ın üzerinde sulama durumu tahmini doğruluğu elde edilmiştir. Diğer algoritmalar da yüksek doğruluk oranları ile (%75 üzeri) dikkate değer performans sergilemişlerdir. Her bir il için yapılan analizlerde, algoritma performans sıralamasının benzer olduğu belirlenmiştir. Sonuç olarak, bu çalışma makine öğrenimi algoritmalarının tarımsal sulamada oldukça yüksek bir performansla kullanılabileceğini göstermektedir.
Afficher plus [+] Moins [-]Synthesis of Quantum Dots Using Biomaterials Derived from Blue Crab and Their Potential Applications Texte intégral
2025
Övgü Gencer
The blue crab (Callinectes sapidus, Rathbun 1896) has become a significant source of raw materials in biotechnology and nanotechnology due to the biomaterials present in its shell. Natural polymers such as chitin and chitosan, derived from the crab's shell, are particularly noteworthy for their environmentally friendly and biologically compatible properties. These biopolymers provide an innovative alternative in the synthesis of quantum dots (QDs). Quantum dots are favored in various applications, including biomedical imaging, environmental sensors, and energy storage, due to their superior optoelectronic properties. Chitosan obtained from blue crab shells acts as both a stabilizer and a coating agent in the green synthesis of quantum dots. This process minimizes the use of toxic chemicals, thus promoting environmental sustainability. Moreover, the antimicrobial and biodegradable properties of chitosan enhance its usability in biomedical applications. For instance, biocompatible carbon-based quantum dots have shown promising results in cancer diagnostics and drug delivery systems. The synthesis of quantum dots using biomaterials is more cost-effective and environmentally friendly compared to traditional methods. Furthermore, utilizing blue crab shells as a waste material contributes to both marine ecosystem preservation and the circular economy. These synthesis methods are reported to create a significant paradigm shift in the field of sustainable technology development. In conclusion, the synthesis of quantum dots using biomaterials derived from blue crabs has the potential to reduce environmental impacts while serving advanced technological applications. This approach significantly contributes to the development of biotechnological innovations and sustainable development goals.
Afficher plus [+] Moins [-]Evaluation of Antioxidant and Anti-inflammatory Potential of Alpinia officinarum with Different Ionic Solutions Texte intégral
2025
Nuray Üremiş | Figen Güzelgül | Ergül Belge Kurutaş
Alpinia officinarum, which attracts attention with its antioxidant and anti-inflammatory properties, is used in traditional medicine, especially to relieve stomach and digestive system disorders. Although many studies have revealed the various pharmacological effects of Alpinia officinarum, the effect of different ionic solvents on its biological activities has yet to be investigated. In this study, the effects of homogenization of Alpinia officinarum roots with potassium chloride (KCl), sodium chloride (NaCl), and phosphate (PBS) buffer solutions on the antioxidant and anti-inflammatory properties of the plant were investigated. Alpinia officinarum plant was collected from the Adana region during the season, and fresh root parts were separated and analyzed. Superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO) enzyme activities, and malondialdehyde (MDA) levels of plant homogenates prepared with KCl, NaCl, and PBS were determined by spectrophotometric analysis. The highest MPO and CAT enzyme activities were observed in the KCl solution, while lower levels were observed in NaCl and PBS solutions, respectively. The highest MDA level was observed in the PBS solution. Moreover, SOD enzyme activity showed a decreasing trend in NaCl, KCl, and PBS solutions, respectively. These findings suggest that the biological activity of plant extracts may vary depending on the solvent used. Determination of the conditions under which the antioxidant and anti-inflammatory effects of Alpinia officinarum in different ionic solvents are the highest supports increasing the bioavailability of the plant.
Afficher plus [+] Moins [-]Eczane ve Aktar Kaynaklı Hypericum perforatum L. (Sarı Kantaron) Yağının Kollajenaz ve Tirozinaz İnhibisyon Aktivitelerinin Değerlendirilmesi Texte intégral
2025
Esra Panayır | Eda Sönmez Gürer | Rengin Baydar | Ayşe Esra Karadağ
Ülkemizde geniş bir alanda yayılış gösteren Hypericum perforatum L. (sarı kantaron) bitkisi halk arasında soğuk algınlığı, depresyon, safra rahatsızlıkları, mesane irritasyonları, migren ağrıları ve yanık gibi pek çok hastalığın tedavisinde kullanılmaktadır. Bu çalışmada; eczane (numune 1) ve aktardan (numune 2) temin edilen iki ayrı sarı kantaron yağının, tirozinaz ve kollajenaz inhibitör potansiyellerini belirlemek amaçlanmıştır. Deneyler kolorimetrik ölçüme dayanan enzim reaksiyonları üzerinden gerçekleştirilmiştir. Her iki numune de tüm deneylerde 20 µg/mL konsantrasyonda çalışılmıştır. Numune 1’in (%18,3±0,15 inhibisyon), numune 2’ye (%4,1±0,28 inhibisyon) kıyasla daha yüksek kollajenaz enzim inhibisyonu sağladığı ve yine numune 1’in (%15,1±0,24 inhibisyon) numune 2’ye (%3,7±0,19 inhibisyon) kıyasla daha yüksek tirozinaz enzim inhibisyonu sağladığı belirlenmiştir. Bu sonuçlar doğrultusunda; sarı kantaron yağının kollajenaz ve tirozinaz enzimlerini inhibe ettiği, eczane kaynaklı sarı kantaron yağının aktar kaynaklı sarı kantaron yağına oranla daha yüksek inhibisyon gösterdiği tespit edilmiştir. Bu durumun, üreticinin bitkiyi doğru zamanda toplaması, uygun şekilde kurutması ve doğru maserasyon yöntemiyle yağ üretmesi sonucunda ortaya çıktığı; bu sayede içerisindeki biyoaktif bileşenlerin yoğunluğunun artarak aktivitenin yükselmesine katkıda bulunmuş olabileceği şeklinde yorumlanabilir.
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