Bu çalışmada, in vitro çoğaltma yöntemiyle nanelerin seri üretimi amaçlanmaktadır. İki farklı nane (Mentha x piperita L., Mentha pulegium L.) türünden eksplant örnekleri alınıp gerekli sterilizasyon işlemi yapıldıktan sonra 100 µL/L NAA (Naphthylacetic Asit) ve 600µL/L IBA ( Indol Butyric Asit) seviyelerindeki hormonlarla MS (Murashige & Skoog) ortamına alınmıştır ve 60 günde iki hafta arayla alt kültür yapılarak aktarma işlemi gerçekleştirilmiştir. Eksplantlardan aynı MS ortamında kök, gövde ve yaprak oluştuktan sonra naneler bitki büyütme odasında toprak ortamına alıştırılmıştır. Nanelerin kallus indüksiyonu 100 µL/L NAA ve 250µL/L BAP (Benzylaminopurine) hormon seviyeleri kullanılarak MS ortamına alınmıştır. Kallus kültürü için ortama alınan eksplantlardan sadece birinde (Mentha pulegium) kallus hücrelerinin indüklendiği görülmüştür. Mikroçoğaltım yöntemiyle geliştirilen her iki Mentha ssp’de (Mentha pulegium, Mentha x piperita) türünde de doğal yolla çoğaltma yöntemine göre ayda 6 kat daha fazla büyüdüğü görülmüştür. Elde edilen verilere göre mikroçoğaltım yöntemiyle geliştirilen Mentha x piperita ve Mentha pulegium türlerinin üretim potansiyelinin oldukça yüksek olduğu tespit edilmiştir.

The present study aimed to determine the heavy metal cadmium (Cd), chromium (Cr), nickel (Ni), lead (Pb) and mercury (Hg) accumulations in muscle tissues, gills, gonads, liver, and other visceral organs (liver) of whiting fish (Merlanguis merlangus euxinus Nordmann, 1840) samples obtained by hunting from the Sinop (Black Sea) coasts. The mean cadmium value in muscle tissue, which concerns public health, was found to be 1.07±0.02 µg.kg-1, the chromium value was found to be 1.48±0.07 µg.kg-1, the nickel value was found to be 1.77±0.14 µg.kg-1, the lead value was found to be 5.38±0.29 µg.kg-1 while the mercury value was determined only in the gill and could not be determined in organs, and since it was below the limit values declared by international organizations for all metals, it was determined that it would not create adverse effects for human consumption. Also, examining the order of accumulation of heavy metals in tissues and organs from highest to lowest, it was sorted as liver> visceral organ> gill> muscle> gonad for cadmium; visceral organ> gill> muscle> liver> gonad for chromium; visceral organ> gill> liver> muscle> gonad for nickel; gill> visceral organ> liver> muscle> gonad for lead, while the mercury accumulation was detected only in the gill. Also, the data obtained as a result of the analyses made in the gonad is an important determination in terms of the effects of heavy metals on the reproduction of the species, as well as on the continuity of the population. Since the values determined in the other analyzed tissues were below the limit values reported by international organizations, the results showed that they do not pose any threat to human health in terms of workplace and time, type, and determined heavy metals.

This study was aimed to evaluate DNA fragmentation by using Comet assay in naturally infected sheep with Anaplasmosis before and after treatment with the Comet method, which shows DNA damage specifically. In the study, blood samples were collected from 10 Anaplosmosis infected and 10 healthy sheep. The anaplosmosis was diagnosed by clinical signs and symptoms. The infection was confirmed by Giemsa staining. The blood was collected from control group and infected group before and after the treatment, from the vena jugularis with the appropriate method. The DNA fragmentation was checked by using the Comet assay of blood cells. The data were analysed throught ANNOVA one-way. The result showed higher DNA fragmentation in sick animals diagnosed with anaplasmosis; tail length and tail moment values were found to be statistically significantly higher than the control group. When the data obtained after imidocarb (IMD) application were compared with obtained during the disease, a decreased DNA damage and tail moment was determined, however, these values higher than control. In this study, DNA damage and the extent of this damage were investigated by the Comet assay method using a healthy control group before and after treatment in animals with Anaplasmosis. When the findings obtained from the study were evaluated, it was seen that Anaplasma agents caused DNA damage and with the imidocarb application given for treatment, DNA damage was reduced and results close to healthy individuals were obtained.

In this study, roots of Turkish origin wild chicory (Cichorium intybus L.) genotypes were investigated for total flavonoid and phenolic contents, radical cation scavenging activity (ABTS), Free radical scavenging activity (DPPH), and radical, mineral content. These characteristics were also compared with other coffee varieties. The total flavonoid and phenolic contents ranged between 0.290-4.350 mg QE/g dry weight (DW) and 0.943-13.860 mg GAE/g DW. The DPPH was listed here from high to low value: raw coffee beans = roasted coffee beans > roasted fruits of turpentine tree > instant coffee = roots of chicory. The content of P, Ca, Mg, Zn, B, Cr, Co and Mo ranged between 0.71-2.78%, 0.25-0.46%, 7.29-20.66, 4.44-11.07, 0.40-1.67, 0.49-5.48 and 5.69-14.46 ppm, respectively. As a result, chicory roots exhibited low antioxidant activity, but higher mineral content compared to the other tested coffee varieties which indicates that chicory could be used a coffee additive.

The need for studies on the nutritional value of plants depends on their composition. The first generation genetically modified Plants (GMPs) have the same composition as their near-isogenic lines. Therefore, they have the same nutritional value, and most of the animal feeding studies have found no significant differences in the production and health parameters of animals that consumed first-generation GMPs in comparison to non-GMPs. Due to the recent production of transgenic plants with specific nutritional properties (second generation GMPs), their use as feed for animals is viewed with skepticism in very many countries. In this critical review, it is concluded that most of these nutritionally improved plants have not shown adverse effects on the performance of various animals compared to their near-isogenic lines and can therefore be considered in the animal feed industry. However, most of the experiments were conducted on laboratory animals. There is a need to conduct them with animals that are mainly consumed by humans, such as ruminants. There is also a need to feed the whole plant to these animals and not just certain parts of the plant to get a clear picture of its overall safety. In addition, there is a need to determine a suitable long-term nutritional and toxicological approach assessment.

Lumpy skin disease is explained as a significant cattle disease affecting cattle of many parts of the world. An outbreak was experienced in different parts of Bangladesh including Barishal for the first time in the last quarter of 2019. This investigation was carried out to know the outbreak scenario of the disease in southern part of Bangladesh. A total of 726 cattle were included in this study covering 50 dairy farms of Barishal region from September 2019 to December 2019. Morbidity rate was found 21% (CI: 18-24%) and mortality was 1% (CI: 1-2%) in the outbreak area. Young animals (24%) and pregnant animals (70%) were significantly more susceptible compared to aged animals (17%) and non-pregnant animals (15%), respectively. Moreover, male and crossbred cattle were slightly more prone to the infection in comparison to their counterparts. About 45% of the affected animals showed nodular and remaining 55% had edematous lesions. Almost 90% of the affected animals were treated with NSAID followed by antibiotics, antihistaminic, steroid drugs and antiviral drugs where only 20% of the affected animals were treated by authorized veterinary personnel. This was the first epidemiological investigation in the mentioned outbreak area which may serve as a baseline for LSD research in the location needed for developing effective control strategy.

Green areas are spaces organized as areas where people can get rid of their monotonous lives and meet their active or passive recreation needs. In order for a society to be physically and mentally healthy, green spaces must fulfil the purposes of establishment. The main material of the research is the green areas of Bosna-Hersek neighbourhood. The questionnaire method was used in the study and the green areas in Konya province Selçuklu district Bosnia and Herzegovina neighbourhood were evaluated in terms of sufficiency, management and usage

Microwave (MW)-hot air (HA) combined drying was applied to white sweet cherries besides solely HA drying at 50, 60 and 70°C in the presence of citric acid, sucrose and freezing pretreatment in this study. Single power level of MW (90 W) was chosen, and drying behavior of all samples was modelled by using eleven thin layer equations. Two-term, rational and sigmoid models were the most suitable models for describing drying phenomena. Effective moisture diffusivities (Deff) ranged from 1.724×10-10 to 5.173×10-10 m2/s in HA drying and from 4.260×10-10 to 1.805×10-9 m2/s in MW-HA drying. Activation energies (Ea) were between 2.785 and 30.541 kJ/mol and 6.929 and 42.101 kJ/mol for HA and MW-HA drying techniques, respectively. Total color change (ΔE) levels of the outer surface of dried cherries were generally higher than the ones of inner surface. Freezing pretreatment had a comparably lower enhancing effect on the total phenolic content (TPC) of HA dried white sweet cherries compared to fresh sample. The TPC of freezing pretreated and HA dried at 50°C and HA dried at 70°C control samples were 1.481 ± 0.398 mg gallic acid equivalent (GAE)/g dry matter (DM) and 6.181 ± 0.012 mg GAE/g DM as the minimum and maximum, respectively. These values were determined as 4.183 ± 1.728 and 8.240 ± 0.502 mg GAE/g DM that were belonged to MW-HA dried at 50°C control and freezing pretreated MW-HA dried at 70°C samples in combined procedure, respectively.