Pseudomonas aeruginosa, belonging to the Pseudomonadaceae family, is Gram-negative, rod-shaped, motile, aerobic, endospore negative, oxidase and catalase positive. It is widely found in nature and isolated from soil, plants, water and animals. It can grow rapidly on the surface of the food and form oxidized products and mucous substances. P. aeruginosa, one of the leading foodborne pathogens, causes important concerns in food safety due to being a source of contamination, causing food poisoning and antimicrobial resistance in animals, forming biofilms and difficulties in preventing biofilms. In this review, information on history, microbiological, cultural and biochemical characteristics, virulence factors and pathogenicity of P. aeruginosa are given. In addition, infections caused by P. aeruginosa and its presence in food are described.

The fall armyworm [Spodoptera frugiperda, (JE Smith, 1797) (Lepidoptera: Noctuidae)] is a polyphagous pest native to tropical and subtropical regions of America. After it was detected for the first time in Nepal in the Nawalpur area on 9th May 2019, it has become a major threat in maize fields even though it has over 80 host species to proceed its life cycle. Due to its migratory nature, FAW moth can travel up to 500 km before oviposition, and infestation of its larva has resulted in vast devastation of the vegetative as well as reproductive parts of plants causing significant yield loss in maize. A mature larva possesses a dark head with an upside-down pale Y-shaped marking on the head area and black four spots that are arranged in a square on the second last body segment. This paper audits the executive choices (avoidance, observing, push-pull, cultural, biological, organic, chemical, and integrated techniques to incorporate in FAW susceptible areas) that apply to smallholder farmers who do not have the monetary asset to buy compound pesticides and other costly control instruments. For the majority of Nepalese farmers with low resources and small landholding, push-pull technology is beneficial and applicable. Botanicals that have bioactive chemical compounds, insecticidal, pest repellent properties are environment-friendly and degradable, readily available in tropical and subtropical regions of Nepal. The assessment of the efficacy of implemented management practices against FAW has revealed that implementation of more than one method of management practices showed the least percentage of infestation as compared to the individual method.

The current research was carried out to determine the effect of adding sodium formate at the level of 0, 5, and 10 g/kg to the diets on the performance, egg quality and some serum parameters in layer Japanese quails. In the experiment, a total of 84 female quails at the age of 20 weeks were randomly distributed to 3 treatment groups with 7 replicates. At the end of the experiment, the addition of sodium formate at different levels to the diet did not statistically affect performance and egg quality parameters. Compared with the control group, the supplementation of 10 g/kg sodium formate to the diet considerably decreased the serum glucose concentration, while the serum calcium concentration increased significantly. Furthermore, the administration of sodium formate to diet significantly decreased serum triglyceride and cholesterol concentrations. According to the results obtained from the study, it was determined that the addition of sodium formate to the diet was effective in reducing serum glucose, triglyceride and cholesterol concentrations and increasing serum calcium concentration without affecting the performance and egg quality parameters of laying quails, and this effect was more pronounced at 10 g/kg sodium formate level.

In this study, the effect of foliar L-Histidine and Methinonin amino acid applications on grain cadmium (Cd) and zinc (Zn) uptake on durum wheat grown in soil contaminated with Cd was investigated. The research was carried out according to the randomized blocks design as a pot experiment in greenhouse conditions. In the experiment, Cd doses were applied as control (0 mg Cd kg-1) and (3 mg Cd kg-1). L-Histidine and Methionine amino acids were applied 7 times from the leaf after the start of flag leaf formation in wheat at 0.5 mM doses and harvested after the grain maturity was completed. According to the results obtained, the wheat grain dry matter yield in 3 mg Cd kg-1 polluted soil was 44.5 mg grain-1 in the control, while the dry matter weight was increased with the application of L-Histidine and Methionine from the leaves being 48 and 50 mg grain-1, respectively. It was determined that there were differences in grain Cd and Zn concentrations with the application of amino acids from the leaves at the dose of 3 mg kg-1 cadmium. When the grain Cd and Zn intakes were compared with the control, it was found that the grain Cd concentration decreased, and the Zn concentration increased as a result of the application of amino acids. The high amount of Cd in wheat can make it to the human body through the food chain and is known to cause serious health problems after a certain amount of accumulation. As a result of this study, it is seen that L-Histidine and Methionine are effective in reducing Cd in the grain, and these amino acids probably form a complex with Cd, resulting in less transport.

This study was designed to investigate the therapeutic effects of Vitis vinifera L. seed extract (5 ppm and 10 ppm) on gill and liver tissues histopathology of carp (Cyprinus carpio L. 1758) exposed to cadmium chloride (CdCl2=Cd) (20 ppb) acute and subchronic period. A total of 140 fish in 14 groups were used in the experiment. The experiment was designed as in two different periods (acute and chronic) and 7 different dose groups (control, vehicle, V. vinifera seed extract 5 ppm, V. vinifera seed extract 10 ppm, Cd 20 ppb, V. vinifera seed extract 5ppm + Cd 20 ppb and V. vinifera seed extract 10 ppm + Cd 20 ppb). At the end of the experiment, the gill and liver tissues dissected from the fish were fixed, dehydrated, cleared, and paraffin impregnated. Appropriately sized blocks were prepared from the tissues, sections of 5-7 µm thickness were taken and covered by staining with hematoxylin-eosin. Stained preparations were examined under a light microscope and photographed. It was determined that all dose groups in the acute period and control groups were not different histopathological. It was determined that the histopathological damage caused by the cadmium group in the subchronic period and tissue damage were reduced in the dose groups with cadmium added with V. vinifera seed extract. As a result of the study, it was concluded that V. vinifera seed extract could have a therapeutic effect on the gill and liver tissues of carp exposed to cadmium.

In this research; the current situation and problems of the cold storages in the province of Van have been studied. In this direction, 21 cold storages registered in Directorate of Van Provincial Agriculture and Forestry were visited and on-site investigations were made. The findings obtained as a result of the surveys made with their owners by considering the features of the cold storages such as the structural and mechanization status, the type of stored product have been effective in identifying problems and suggesting solutions. It is used for white meat and eggs 29% of cold storages, 29% for milk and dairy products, 29% for red meat and 13% for fruit and vegetable storage. In addition, there are 17 fruit and vegetable warehouses, 13 of which are unused, that are not registered in Directorate of Van Provincial Agriculture and Forestry, and these were excluded from the research because they were not registered. The most important problems in warehouses were experienced during the Covid- 19 pandemic process. Lack of the technical capacity, machinery and equipment, qualified personnel, the marketing problems, maintenance and repair, rent, electricity and water expenses are the main ones. Improvement of its structural properties, modern mechanization device, establishment of unions that will enable warehouse owners to act together, eliminating the lack of qualified personnel with Van Yuzuncu Yil University, one of the most important educational institutions in the region and measures to increase export potential will play an important role in solving the problems.

Rice (Oryza sativa L.) is the most important staple cereal crop which is consumed by more than 50% of world population. It contributes 23% and 50% of total calories consumed by world and Nepalese population respectively. Among various abiotic factors affecting rice, rice blast is the most disastrous, causing 70-80% yield loss. This disease was originated in China around 7000 years ago. In Nepal, it was first reported in Thimi, Bhaktapur in 1966. It is caused by a filamentous ascomycete fungus Magnaporthe oryzae (Anaemorphic form- Pyricularia oryzae). It infects all the developmental stage of plant and produce symptoms on the leaf, collar, neck, panicle and even in the glumes. It decreases the rice production by an amount, enough to feed 60 million people every year. Cloudy weather, high relative humidity (93-99%), low night temperature (15- 20°C), longer duration of dew is the most favorable condition for the outbreak of disease. The most usual approaches for the management of rice blast diseases are management in nutrient fertilizer and irrigation, application of fungicides and plantation of resistant cultivars. Besides, the use of extracts of C. arabica are reported to have an inhibitory effect on the disease. Seed treatment with Trichoderma viridae @ 5ml/lit of water have also been found effective. The chemical means of controlling blast disease shall be reduced, instead eco-friendly measures like biocontrol agents, resistant varieties, plant extracts can be practiced for disease control. Different forecasting model can be used in order to predict the disease prevalence.

The objective of this study is to evaluate the antioxidant, antiulcer, anti-inflammatory and analgesic activity of the aqueous extract of Angelica archangelica L., a medicinal plant of the traditional pharmacopoeia of Algeria. The aqueous extract showed a large amount of total polyphenols, tannins, chlorophyll a, b, carotenoids and a small amount of flavonoids with values of 80.16±14.3 mg EAG/g of extract, 3.12±2.01 mg EQ/g of extract, 226.10±4.50 mg EAT/g of extract, 6.10±50.62, 12.00±31.53 and 1.78±0.40 μg/mL respectively. In addition, the aqueous extract of A. archangelica showed a high ability to trap DPPH radical in with IC50 at 16.9±2.03μg/mL. However, the aqueous extract has a great protective effect against β-carotene degradation at 91.98±0.64% after 24 hours and significant ferrous ion chelation activity at46.2 ±1.53µg/mL. Furthermore, the anti-inflammatory activity was studied according to the protein denaturation inhibition method and, according to the results obtained, the extract of A. archangelica at concentrations of 250, 500 μg/mL and 1 mg/mL have ahigh antiinflammatory activity whose inhibition percentages are 78.85±5.31%, 86.65±2.70% and 89.89±0.58% respectively. The analgesic effect of the aqueous extract of A. archangelica, showed that the concentration 400 mg/mL has a greet abdominal cramps inhibitory effect in comparison with the concentration 200 mg/mL with a percentage of 98.28%. The antiulcer effect of the aqueous extract of A. archangelica was evaluated by the 70% ethanol-induced ulcer test. The results obtained reveal that the aqueous extract 200 and 400 mg/mL exerted a considerable effect of protecting the stomach at 86.55±3.51% and 82.82±2.18% respectively.

Purple basil is widely used medicinally and aromatically due to its essential oil properties, and it is a plant that stands out with its antibacterial, antimutagenic and antioxidant properties. It has been determined that purple basil has positive effects on health such as appetizing, insomnia, sputum, diuretical and antispasmodic. Purple basil contains high concentrations of anthocyanins and with these properties it stands out as a potential new source of stable red pigments for the food industry. In recent years, it has been seen that purple basil has been processed into different products such as tea and sherbet in order to benefit from its functional properties. With the processing of purple basil into different products, it is seen that the consumption of basil and basil products has increased by consumers. In this study, purple basil sherbet samples were concentrated at different voltage gradient values (14, 17 and 20 V/cm) under atmospheric conditions with ohmic heating up to 25% soluble solid content (SSC) and the changes in their rheological properties during the concentration process was investigated. The changes in the rheological properties of the purple basil samples were determined in the range of 1-148 s-1 shear rate values and measurement temperature was 25°C. The compatibility of different rheological models (Newton, Power-Law and Herschel-Bulkley) to the experimental data was statistically evaluated to determine the flow behavior index of the purple basil sherbet samples. It has been determined that the shear stress values increased as the shear rate values increased in all the process conditions. Similarly, at the same shear rate values, it was determined that the shear stress values increased as the SSC values increased. It has been determined that the best suitable rheological model was Herschel-Bulkley Model for all purple basil sherbet concentrates. It is thought that the results obtained will provide important data to the food and machinery industry for the installation of pilot and industrial scale ohmic heating systems.

The aim of this study is to contribute to the valorization of the cocoa bean varieties “Mercedes” and “Forastero” grown in Côte d’Ivoire. A comparative characterisation of phenolic compounds and minerals in the beans of the “Mercedes” and “Forastero” varieties from two major cocoa production areas of Côte d’Ivoire were determined. The phenolic compounds of cocoa beans were determined by high performance liquid chromatography (HPLC) and the minerals by atomic absorption spectrophotometer. The study showed that a significant difference at the 5% level was observed in the averages of minerals, cinnamic acid, protocatechic acid, quercetin, coumaric acid, rutin, ellagic acid, veratric acid, epicatechin, ferulic acid and naringenin. On the other hand, no significant difference at the 5% level was observed in the averages of arbutin, catechin, vanillic acid and caffeic acid in the bean kernels analysed in the Divo and Abengourou regions. The proportion of cinnamic acid is (6.18±0.63 mg/100g “Mercedes” Divo), that of ellagic acid is (18.48±0.44 mg/100g “Mercedes” Divo) and that of naringenin is (5.95±0.35 mg/100g “Mercedes” Divo). The amount of potassium is (7569.97±1.51 mg/kg “Forastero” of Abengourou) and that of iron is (179.9 ± 0.70 mg/Kg “Forastero” of Divo). The phenolic compounds and minerals in the cocoa bean samples analysed are highly correlated and rich in antioxidants. They would be beneficial for many biological functions. They can be recommended in pharmacology and cosmetics to fight against oxidative stress and cardiovascular diseases.