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Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew Texte intégral
2022
Ulku Baykal | Kadriye Özcan
Powdery mildew affecting European hazelnut Corylus avellana L. trees in Turkey is caused by the obligate biotrophic fungus Erysiphe corylacearum. This fungal disease causes significant economic losses by reducing the yield and quality of hazelnuts. Loss-of-function mutations in the mildew resistance locus o (MLO) gene family of many plants confer high levels of broad-spectrum resistance to powdery mildew. The proteins encoded by the genes at the MLO locus are divided into approximately seven different conserved clades. Among them, the phylogenetic clade V has been shown to be involved in PM susceptibility, as inactivation of these genes leads to long-term disease resistance in dicotyledons. In this study, we examined the temporal expression pattern of three hazelnut MLO genes, previously identified as clade V, in response to powdery mildew infection in C. avellana cv. Tombul. Leaves are the main tissue affected by the powdery mildew pathogen in hazelnut plants. Analysis of MLO expression in hazelnut leaves showed that CavMLO2 and CavMLO6 were significantly upregulated after challenge with E. corylacearum, providing preliminary evidence that they may be involved in PM susceptibility. Thus, these results present a basis for the isolation and use of relevant genes in plant breeding for disease resistance. Moreover, expression profiles of the clade V MLO genes are also important to identify candidate genes that need to be silenced or edited for future molecular studies to obtain resistant hazelnut varieties.
Afficher plus [+] Moins [-]Connection of Fisheries Management and Ballast Water Management Contract (Bwm Convention, 2004) Texte intégral
2022
Serpil Yilmaz | Esra Erikçi Bilgin
Although land-based pollution is expressed as the most important pollution factor in the pollution of the seas (80%), the rate of pollution originating from ships (approximately 20%) is not to be underestimated. The fact that marine pollution is caused almost exclusively by ships requires investigation of the measures to be taken against marine pollution from ships and their applicability. As a matter of fact, the most important cause of pollution caused by ships is ballast water, and it has been determined that over 7,000 marine organisms are transported between ports with ballast waters on a global scale every day. Ballast water is used to maintain the balance of the ship on the water when the ships are not loaded. Considering that 90% of international trade is still provided by ships and 3-5 billion tons of ballast water is transported by approximately 85,000 ships every year with world maritime trade, the importance of pollution caused by ballast water becomes apparent. While ballast water is one of the elements that cause the transport of exotic marine and freshwater organisms between all seas of the world and cause pollution, the pollution in question is very effective especially in the aquaculture sector. Therefore, in this article, the connection of ballast water with fisheries management has been examined and solutions have been sought for the problems.
Afficher plus [+] Moins [-]Factors Affecting Colony Losses in Honey Bees (Apis mellifera) Texte intégral
2022
Halit Yücel | Kübra Ekinci | Altuğ Karaman | Halil Yaninar | Hülya Koca Karaman
Honey bees are a very important species in terms of economy, agriculture, and environment. In recent years, there has been a significant reduction in honey bee colonies in some parts of the world. Honeybee losses are not an unusual event, but there has been a significant reduction in honey bee colonies in many countries around the world. Due to the different social behaviors of honey bees, it is difficult to identify the main factors causing colony losses. According to the latest research, colony losses are mainly caused by parasites, diseases, bee keeping practices, and bee management including reproduction, changes in climatic conditions, agricultural practices and pesticide use, pesticides, nutrition, and beekeeping practices. In this review, the structure of prebiotic, probiotic, climate change, and vitollegen, which causes colony losses, is emphasized, and the potential solutions of these factors that will shed light on colony losses in honey bees from a different point of view are emphasized. In addition, bibliometric analysis was performed using the SCOPUS database to emphasize the importance of probiotic microorganisms and vitellogen.
Afficher plus [+] Moins [-]Investigation of TLR2 -EcoRV, NOD2- BsaHI ve IFNγ- HphI allele frequency distribution among Turkish Native Cattle Breeds Texte intégral
2022
Yasemin Öner | Nezih Ata | Mustafa Karabaş | Onur Yılmaz
Changing climatic conditions and growing population size lead take precautions against potential risks. Researchers who study in the agricultural field, including animal production, focus on finding out variants and breeds resistant to diseases and environmental stress. Detecting gene regions that affect resistance to diseases and environmental stress might increase the performance of the selection in favor of these traits. Due to these reasons in this study, five Turkish native cattle breeds were investigated for SNPs belonging to TLR2, NOD2, and IFNγ (using EcoRV, BsaHI ve HphI enzymes, respectively) reported to be associated with paratuberculosis previously. In total, 169 animals were analyzed from Native Southern Yellow (NSY= 29), East Anatolian Red (EAR=35), Anatolian Grey (AG=36), South Anatolian Red (SAR=34), and Native Black (NB=35). The genotype and allele frequencies were calculated with the PopGene32 program, and Khi square test was performed to determine whether the populations were in Hardy-Weinberg equilibrium (HWE). While at TLR2- EcoRV and NOD2- BsaHI loci, two alleles (A and C, C and T, respectively), IFNγ- HphI locus was found to be monomorphic for the G allele. For TLR2- EcoRV locus, the C allele was found as predominant except NB breed, and the C allele was predominant for all breeds at NOD2- BsaHI locus. However, in all breeds, NOD2- BsaHI locus was at HWE, for TLR2- EcoRV NSY and SAR breeds showed derivation from HWE.
Afficher plus [+] Moins [-]General Properties and Effects of Some Husbandry Practices on Milk Yield in Dairy Farms of Ondokuz Mayıs District of Samsun Province Texte intégral
2022
Murat Satılmış | Savaş Atasever
In this study, it was aimed to reveal the structural properties and the effects of husbandry practices on milk yield in dairy farms of Ondokuz Mayıs district of Samsun. While the data were obtained by face-to-face surveys and observations in a total of 59 farms those had ≥10 head cattle between January and June 2018, environmental factors affecting daily milk yield (DMY) were separately evaluated. Important negativeneses were determined on the barn type, bedding material using, calving pens, weaning period of calves, claw care and udder cleaning in the farms. It was informed that local bazaars and cooperatives were commonly used for raw milk marketing, and high feed price was the most important problem. DMY was significantly affected by the experience of farm owner, number of the milking cows, amount of concentrate feed that given and milking type. It was concluded that farm properties and herd management methods have to be restored to elevate milk production in the investigated farms.
Afficher plus [+] Moins [-]Free Radical Scavenging and Antinociceptive Activities of the Aqueous Extract from Matricaria chamomilla L. Flowers. Texte intégral
2022
Chahrazed Kaoudone | Fatima Benchikh | Cherif Abdennour | Hassiba Benabdallah | Walid Mamache | Smain Amira
Background: Matricaria chamomilla L. (M. chamomilla) is a famous medicinal plant distributed worldwide. It is widely used in traditional medicine to treat all kinds of diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders. It is also used as a sedative, antispasmodic, antiseptic, and antiemetic. Our aims in this study was thus to quantify the phenolic, flavonoids contents in the flower of this plant, and also to evaluate the in vitro antioxidant potential and the in vivo analgesic activity. Methods: The total phenolic and flavonoid contents of the plant aqueous extract (MCAqE) were estimated using the Folin–Ciocalteu and AlCl3 colorimetric methods, respectively. However, DPPH method was used to evaluate the in vitro antioxidant activity. Analgesic activity was tested by acetic acid induced writhing model in mice. Results: Quantitative determination of total polyphenols and flavonoids revealed that this extract contained 158.41±1.6 mg gallic acid equivalent/g of dry extract and 37.06±0.56 mg quercetin equivalent/g of dry extract, respectively. The antioxidant activity of the plant extract was important (IC50=3.08±0.25 mg/mL). MCAqE extract, at 400 mg/kg, showed analgesic activity (39.60±8.70%) against acetic acid induced pain in mice while the standard reference drug Diclofenac sodium exhibited 90.44±2.80% activity at 10 mg/kg dose.
Afficher plus [+] Moins [-]Policy Recommendations for The Effects of the Covid-19 Outbreak on Seasonal Agricultural Workers: The Case of Türkiye Texte intégral
2022
Merve Bozdemir Akçil | Zeki Bayramoğlu
Impact of Covid-19 pandemic introduced with itself some socio-economic transformation as well as a myriad of problems. Closure of borders, alongside a fall in trade and human mobility brought forward concerns related to the self-sufficiency of nations. Throughout the pandemic outbreak, there was an emerging need for the countries to harness their own manufacturing resources and capacity efficiently to answer soaring demands of domestic market. At this point, visibility of agricultural sector has become foregrounded because of its inherent role in food and nutrition. Particularly speaking; producers who supplied need for seasonal labor from other countries were forced to take instantaneous measures and implement political changes. In Türkiye, since demand for seasonal labor could be met through domestic resources, it was feasible to prevent any failures during manufacturing process. Nevertheless, there were common concerns arising among the public on the measures to take for the work and life conditions of seasonal agricultural workers and required policies to implement. Within the context of this study, research on the discussions, practices, and measures to follow for agricultural sector during Covid-19 pandemic period has been conducted. Analyses have been drawn on the regulation in Türkiye and relevant policies have been suggested.
Afficher plus [+] Moins [-]The Determinants of Agricultural Output Growth in Ethiopia Texte intégral
2022
Gudina Goda Korsa | Jiffara Abdissa Labata
The purpose of this paper is to look into the determinants of agricultural output growth in Ethiopia. Along with this general objective, this study intends to look at the trend of total factor productivity growth in the agricultural sector and its contribution to agricultural output growth. Using autoregressive distributed lag model bounds testing, this research estimates the long-run and short-run cointegration between agricultural output growth and the total factor productivity. In this study, Augmented Dickey-Fuller and Phillip-Perron unit root tests were used to find out the order of integration of the variables. The selected econometric model goes through all the diagnostic tests and confirms the absence of heteroscedasticity, serial correlation, and normality. The finding of this study indicates that total factor productivity, agricultural land, agricultural machinery, and fertilizer all have a significant and positive effect on the growth of agricultural output. According to the findings of this study, total factor productivity is the primary driver of agricultural output growth. Therefore, as it has played a strong role in developed countries' agriculture, total factor productivity has the potential to be a game-changer in terms of sustainable agricultural growth. Taking into account the findings of this study, we strongly recommend that the government of Ethiopia should devise policies in the agricultural sector that could enhance the level of total factor productivity.
Afficher plus [+] Moins [-]Determination of Fatty Acid Content of V. opulus Grown in Sivas, Kayseri and Yozgat Texte intégral
2022
Alper Zöngür
The common name for Viburnum opulus in Türkiye is "gilaburu". Despite its bitter and acrid taste, it is consumed as fruit juice or products such as jam, marmalade, jelly. They have antioxidant properties as they contain high levels of phytocompounds such as anthocyanin, phenolics, triterpenoids and vitamins. In the study, the fruits of V. opulus grown in Sivas, Kayseri and Yozgat villages were found to contain palmitic acid (C 16:0), oleic acid (C 18:1), linoleic acid (C 18:2), myristic acid (C 14:0), palmitoleic acid (C 16:1), stearic acid (C 18:0), heptadesanoic acid (C17:1), eicosenoic acid (C 20:1), eicosadienoic acid (C 20:2), lauric acid (C 12: 0), margaric acid (C 17:0), pentadecanoic acid (C 15:0), caprylic acid (C 8:0) and gincoloic acid (C 15:1) were examined. In the study, it was observed that the dominant fatty acids were palmitic acid (C 16:0), oleic acid (C 18:1) and linoleic acid (C 18:2). Similarly, in the examples, lauric acid (C 12:0), margaric acid (C 17:0), pentadecanoic acid (C 15:0), caprylic acid (C 8:0) and gincoloic acid (C 15:1) found in trace amounts.
Afficher plus [+] Moins [-]Serological Detection of Three Common Tomato Viruses in Tomato Cultivation Areas of Ereğli District, Konya Texte intégral
2022
Serkan Yeşil
In this study, it was aimed to reveal some viruses that are a problem in tomato production areas in the Ereğli district of Konya, where tomato production is common. For this reason, in the 2019 production season, leaf samples were taken from 160 tomato plants showing symptoms similar to virus disease symptoms such as mosaic, deformations, yellowing, blight on the leaves and stunted plants. Possible infections of Cucumber mosaic virus (CMV), Tomato spotted-wilt virus (TSWV) and Tomato mosaic virus (ToMV) on these leaves were tested by serological-based DAS-ELISA (Double Antibody Sandwich- Enzyme Linked Immuno Sorbent Assay) method. According to DAS-ELISA results, it was determined that 66.25% of the samples were infected with at least one of these three viruses. In addition, TSWV was detected as the most common virus with a 48.75% relative infection rate in the samples, followed by ToMV (28.75%) and CMV (26.25%). Mixed infections of the viruses were also detected in the leaf samples tested. CMV + TSWV double infection was detected in 22 samples, ToMV + TSWV double infection was detected in 14 samples, and CMV + ToMV + TSWV triple infection was detected in 12 plant samples.
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