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Tuz Stresi Altında Marul Bitkisinin Gelişimi ve Bazı Besin Maddesi İçerikleri Üzerine Organik Materyallerin Etkisi Texte intégral
2023
Ceyhan Tarakçıoğlu
Bu çalışmada, farklı organik materyallerin tuz stresi altında yetiştirilen İceberg ve Kıvırcık marul bitkisinin gelişimi, bazı besin maddesi içerikleri ve stres tolerans parametreleri üzerine etkisi araştırılmıştır. Toprağa vermikompost, fındık zuruf kompostu, düşük ve yüksek kaliteli leonardit bazlı organik toprak düzenleyicileri %2 oranında uygulanmıştır. Tuzluluk stresi üç seviyede (0, 20 ve 40 mM NaCl) gerçekleştirilmiştir. Deneme tesadüf parselleri deneme desenine göre 3 tekerrürlü olarak serada yürütülmüştür. Tuzlu koşullar altında bitkilerin kuru ağırlığı, membran geçirgenliği, nitrat içeriği, K/Na ile Ca/Na oranları düzenli olarak azalırken; prolin, Na, Cl ve Ca içerikleri artmıştır. Stres tolerans parametrelerine göre değerlendirildiğinde, bitkilerin membran geçirgenliği, prolin, nitrat, K, Na, Ca içerikleri ve Ca/Na oranlarının fındık zuruf kompostu uygulamasında, yaş-kuru ağırlık ve Cl içeriklerinin vermikompost uygulamasında en iyi olduğu tespit edilmiştir. Sonuçlarımız fındık zuruf kompostu ve vermikompostun leonardite kıyasla tuzluluk stresinin etkilerini azalttığını göstermektedir.
Afficher plus [+] Moins [-]Gemlik Çeşidi Zeytin Fidanının Su Stresine Bağlı Bazı Morfolojik Ve Fizyolojik Değişikliklerinin Belirlenmesi Texte intégral
2023
Yasin Mercan | Talih Gürbüz
Bu araştırma, tarla koşullarında saksılarda yetiştirilen dört yaşlı “Gemlik” çeşidi zeytin fidanları üzerinde yapılmıştır. Araştırmada; farklı sulama uygulamalarında morfolojik ve fizyolojik parametreler göz önüne alınarak zeytin fidanlarının kuraklık stresine karşı tepkileri incelenmiştir. Araştırma, 2021 yılında, Aydın Adnan Menderes Üniversitesi Ziraat Fakültesine bağlı Araştırma ve Uygulama Çiftliğinde gerçekleştirilmiştir. Deneme tesadüf parseli deneme desenine göre 3 tekerrürlü olarak kurulmuştur. Sulama konuları, bitki su tüketiminin %20 (I20) %40 (I40) %60 (I60), %80 (I80), %100 (I100) ‘ü kadar su uygulanması şeklinde oluşturulmuştur. Çalışma sonucunda; sulama sezonu boyunca konulara uygulanan sulama suyu miktarları 16.32-81.57 L saksı-1 ve buna karşılık elde edilen bitki su tüketimi değerleri ise 19.49-85.08 L saksı-1 arasında değişmiştir. Konulara göre bitki boyunun 3.50 cm ile 11.00 cm, sürgün uzunluğunun 0.14 cm ile 12.4 cm, gövde çapının 0.70 mm ile 2.11 mm, sürgün çapının 0.53 mm ile 1.84 mm arasında değiştiği saptanmıştır. Konulara göre elde edilen yaprak oransal su içeriği değerleri %52.61 ile %71.68, stoma iletkenliği değerleri 260.29 mmol m-2 s-1 ile 535.80 mmol m-2 s-1, klorofil miktarı değerleri 78.00 ile 82.76, yaprak sıcaklığı ve hava sıcaklığı farkı ise 1.93°C ile 5.31°C arasında değiştiği belirlenmiştir. Sonuç olarak, Gemlik çeşidi zeytinin topraktaki nem eksiğine duyarlı bir bitki olduğu, morfolojik ve fizyolojik parametrelerin yetişme mevsimi boyunca sulama suyuna bağlı olarak değiştiği, bu parametrelere ilişkin elde edilen bulgular göz önüne alındığında 4 günde bir eksilen nemin %60’ının (I60) uygulanması önerilebilir.
Afficher plus [+] Moins [-]Toprak Toplulaştırmasının Kırsal Kalkınma ve Sürdürülebilirliğe Etkisi Texte intégral
2023
Derya Balcı | Sema Gün
Toprak toplulaştırması terimi ilk olarak on dördüncü yüzyılda Almanya’da kullanılmasına rağmen toprak parçalılığını çözmek üzere uygulamaya geçirilmesi on sekizinci ve on dokuzuncu yüzyılda olmuştur. Geleneksel olarak uygulanma biçimi aslen toprak parçalılığı sorununu çözmek olan toprak toplulaştırmasının amacı, kapsamı ve işlevi zamanla genişlemiştir. Modern uygulama biçimleri tarım, çevre, kırsal peyzaj ve köylerin yenilenmesi gibi daha geniş bir yelpazeye yayılmıştır. Bu çalışmada, toprak toplulaştırmasının tarihsel süreçte geçirdiği evrim ve bu evrimle birlikte amaç, kapsam ve işlevlerindeki farklılaşmanın kırsal kalkınma ve sürdürülebilirliğe etkileri değerlendirilmiştir. Çalışma, toplulaştırma uygulamalarının amaçlarının zamanla kırsal kalkınma araçlarına dönüştüğünü ortaya koymaktadır. Böylece toprak toplulaştırması, kırsal kalkınmayı sağlayacak önlemlerin yerine getirilmesi aracı olarak kırsal kalkınmayı ve sürdürülebilirliği destekleyen uygulamaları ortaya çıkarmıştır.
Afficher plus [+] Moins [-]Molecular Investigation of the Impact of Thermal Processing Techniques on Tropomyosin Crustacean Allergens Texte intégral
2023
Elif Tuğçe Aksun Tümerkan
While shellfish species are widely consumed due to their nutritional advantages, they are also among the top eight food items for food-borne allergies. Five distinct thermal processing techniques were applied to the crustacean to investigate the tropomyosin level variations caused by heat processing. Fresh shrimp and prawns were utilized as controls for the determination of allergen-encoding genes. Prior to molecular analysis, the proximate composition and acidity of raw and processed samples were also performed. The yield and purity of DNA were also determined. Melting curve and gel electrophoresis tests verified the existence of allergen-coding genes. Thermal processing procedures affected the proximate composition, particularly the total protein and fat concentrations, according to the findings. Following the heat treatment, the pH levels decreased, particularly in the grilled samples. There were also significant differences in the quantity and quality of the extracted DNA. Regardless of crustacean species, the tropomyosin-encoding gene was detected in both fried and grilled samples. These findings demonstrated that RT-PCR identification and validation of the crustacean allergy gene by gel electrophoresis might be a reliable approach for the thermally treated shrimp and prawn samples. This study shows that investigating the allergen coding gene might provide a viable way for detecting food-borne allergens in other thermally processed food items, which are becoming more concerned about food safety.
Afficher plus [+] Moins [-]Acute and Subacute Toxicity of Ruta Montana Extract to Female Rats: Effect on Liver, Kidneys and Ovaries Texte intégral
2023
Mahdeb Nadia | Khadidja Attafi | Souha Bouhouhou | Allouni Rima | Abdelouahab Bouzidi
Ruta montana L. is an annual aromatic plant of the family rutaceae. Quantitative analysis of the methanolic crude extract of Ruta montana L. yielded 8.43%, whereas the qualitative analysis revealed the presence of alkaloid or coumarin. The Litchfield and Wilcoxon method calculated the LD50 of the crude methanolic extract of Ruta montana L. in Wistar albino female rats at 393.18 mg/kg. This allows the plant to be classified as moderately toxic. The subacute toxicity study of the methanolic crude extract of Ruta montana L. in female Wistar albino rats treated with 100 mg/kg (≈ 1/4 LD50) and intraperitoneally showed a significant increase in body weight of the rats treated at the 4th week. Animals treated and sacrificed after 30 days showed a disturbance of the relative mass of the organs. Biochemical parameters of hepatic function assessment showed a significant increase in PAL with elevation of AST and ALT, whereas those of renal function revealed a significant decrease in creatinine with an increase in urea. Hematologic parameters recorded a decrease in RBC, HGB and HCT. The histological sections of the treated rats reveal the existence of blood congestion in the central veins and liver tissues, foci of necrosis and steatosis in the liver, blood congestion and some glomerular atrophy in the kidneys, as well as blood congestions and developed follicles without oocytes in the ovaries.
Afficher plus [+] Moins [-]Farmers' Views on Organic Grape Production in Adıyaman Province: Method Adoption and Problems Texte intégral
2023
Aybüke Kaya | Songül Salık
Healthy life in a sustainable environment is possible with accessible food production. The Covid-19 epidemic is a serious threat worldwide. This epidemic has revealed the importance of agricultural products. One of the most important areas of the epidemic affecting the whole world is exports; however, increased demand for agricultural products and exports in the epidemic process in Turkey. Turkey has a say in the trade of seedless dried grapes in the world. Seedless dried grape is on the healthy products list of the WHO. In 2019/2020, it had a share of 36% of world exports (with 246 thousand tons of export). This study was performed to evaluate their thoughts and farmers’ problems with the adoption of organic farming in Besni district of Adıyaman. The research conducted in-depth interviews with the farmers producing organic grapes. Also, a face-to-face survey was conducted with 50 farmers. According to the findings, the land width of the farmers is not much. It has an average growing area of 37.52 decares. An average yield of 1.808 kg da-1 is obtained from this area. Serious differences have been found between conventional and organic farming. Moreover, government supports, high product prices, income, health, sustainability, and environmental protection are reasons farmers switch to organic farming. However, farmers argue that organic farming is less costly than conventional farming. Access to chemicals and marketing are major problems. As a result, farmers should be informed about organic farming. Additionally, it is thought that these studies will increase the productivity and product quality of the farmers. It is predicted that it will prevent rural to urban migration in the region.
Afficher plus [+] Moins [-]Comprehensive Evaluation of the Clinical Efficacy of an Anti-Diabetic Polyherbal Formulation Texte intégral
2023
Hasib Sheikh | Md. Khairul Alam | Md. Abdus Samad
Background: There are several clinical studies examining the health advantages of several single medicinal herbs utilized in traditional blood glucose-lowering treatments. But very few or no studies on herbal formulations were made as Polyherbal for the same goal. As a result, it is now necessary to confirm that patients with hyperglycemia can benefit from such Polyherbal medicines as Dolabi. Methods: This prospective open-label, herbal coded test drug-controlled, randomized trial was conducted at the Munshiganj and Dhaka area in Bangladesh. We enrolled 108 male and 104 female patients of 30-70 years with primary and moderate hyperglycemia. They were recruited from the OPD of an Unani & Ayurvedic hospital in Munshiganj and different Unani clinics in Dhaka, Bangladesh after fulfilling the inclusion criteria. Patients were randomly assigned to receive metformin hydrochloride 500 mg two times daily and 2 tablets of Dolabi two times daily by using a random numbers table with the help of an assistant. Blood samples, height, weight, blood pressure, and personal data were recorded—laboratory results were obtained at the study baseline, after 1.5 months and after 3 months of intervention. Results: In the case of the test drug, results showed a significant decrease in blood glucose level between the baseline and after 3 months, in males, it was from 9.83±1.17 to 7.72±1.06 mg/dL for fasting glucose, from 16.60±2.35 to 8.23±1.17 mg/dL for 2 hours PP glucose, from 9.33±1.17 to 7.45±2.03 percent for HbA1c and for Insulin it reduces from 183.10±27.59 to 168.10±29.59 pmol/ L. The control drug metformin hydrochloride also showed a significant decrease in blood glucose level between baseline and after 3 months, in the case of males it was from 9.99±2.52 to 6.97±1.76 mg/dL for fasting glucose, from 17.43±5.05 to 7.89±2.42 mg/dL for 2 hours PP glucose, from 10.43±2.36 to 6.87±1.18 percent for HbA1c and for Insulin it reduces from 198.75±30.61 to 183.75±30.61p mol/L. In the case of females the test drug showed a significant reduction in fasting glucose, 2 hours PP glucose, HbA1c and Insulin between the baseline and after 3 months, it was from 10.02±1.11 to 7.78±0.93 mg/dL, from 16.88±2.21 to 8.16±1.11 mg/dL, from 9.84±1.04 to 7.45±1.03 percent and from 199.47±30.90 to 173.47±30.90 mg/dL respectively. In the case of females, the control drug showed a significant reduction in fasting glucose, 2 hours PP glucose, HbA1c and Insulin between baseline and after 3 months, it was from 10.18±1.92 to 6.71±1.59 mg/dL, from 18.70±3.88 to 7.60±3.74 mg/dL, from 10.58±1.08 to 6.98±1.08 percent and from 200.00±31.83 to 188.00±31.83 mg/dL respectively. Conclusions: We can infer the following from the present study’s findings: The polyherbal formulation Dolabi is able to reduce the blood glucose level. It can be an effective drug for primary hyperglycemic patients.
Afficher plus [+] Moins [-]Nutritional Composition and Apparent Metabolizable Energy (AME) Value of Black Soldier Fly Larvae (Hermetia illucens L.) Full-Fat Meal for Broiler Chickens Texte intégral
2023
Dumeshi Ellawidana | Ruvini Kamalika Mutucumarana | Darshanee Ruwandeepika H.A. | Manjula Sumith Magamage
Black soldier fly larvae (BSFL; Hermetia illucens L.) is a proven high-cost protein source replacer and could be grown in a range of bio-degradable waste materials where hardly incorporated into broiler diets locally. The present study was aimed to assess the nutritional composition of BSFL, and apparent metabolizable energy (AME) value of BSFL meal provided to broiler chickens. BSFL full-fat meal produced from kitchen waste as a substrate were examined for their proximate composition, minerals and fatty acid profile. Eighty, 21-d old unsexed Cobb-500 broiler chickens (BW±SD: 665.8 ±14.3 g) were assigned randomly into 16 battery cages (04 replicates, five birds/replicate). A maize-soybean meal-based diet was used as the basal diet which was partially substituted by pre-analyzed BSFL meal at the rates of 5%, 10%, and 15% to produce three test diets. Birds were fed in a completely randomized design for 7-d with a 4-d adaptation period. Excreta were collected for three days from day 25 to 28. The results envisaged that the crude protein (CP) and ether extract (EE) contents of the kitchen waste were 12.3%, and 10.5%, respectively. BSFL meal when analyzed had 34.4% CP and 47.3%, EE. The fatty acid (FA) profile of the kitchen waste was more or less similar to that of BSFL‘s meal. The estimated AME of the BSFL full-fat meal fed for broilers was estimated to be 15.7 MJ/kg. The BSFL full-fat meal can be utilized sustainably in feed formulation and has a high potential to replace costlier feed ingredients.
Afficher plus [+] Moins [-]Polygalacturonase Production by Sarocladium strictum T4 Isolate using Apricot Pulp as Substrate in Non-Sterile Culture Conditions Texte intégral
2023
Ruhşen Aydın Karaağaç | Mehmet Nuri Aydoğan
In this study, 34 molds showing pectin degradation activity were isolated from the soil of orchards, by several tests. By using these isolates, pectinase group enzymes; studies on pectin lyase and polygalacturonase production were performed in the batch system and under non-sterile culture conditions. 5 isolates denoting polygalacturonase activity were coded as: T1, T2, T3, T4, T5 and the best polygalacturonase activity among these isolates was determined as 354.4 U/L in T4 isolate. Thus, optimization analyses continued by the use of this isolate. Initial apricot pulp concentration, temperature, pH and incubation period were tested as optimization parameters. The effects on enzyme activities were investigated by changing the initial apricot pulp concentration in the range of 5-100 (g/L), and in this regard, polygalacturonase activity was determined as 397.4 U/L at 50 g/L. In addition, the pH parameter was analyzed in each unit between pH = 3 – 8, and the temperature was tested by increasing 5 units in the range of 5-25°C. Consequently, the maximum polygalacturonase activity was determined as 405.7 U/L at pH 5 and 406.3 U/L at 15°C. Besides, the effect of the incubation period was studied within 1-5 days and the maximum polygalacturonase activity was determined as 429.0 U/L on the 4th day (after 96 hours). As a result, the above-mentioned T4 isolate, with which the optimization studies were conducted, was identified as Sarocladium strictum (Top ekinküfü) T4 by molecular methods.
Afficher plus [+] Moins [-]Effect of Time and Temperature Storage on the Quality of unpasteurized Prickly Pear Juice Enriched with Hydro-soluble Opuntia ficus indica seeds Extract Texte intégral
2023
Amira Sakhraoui | Noureddine Touati | Soraya Hihat
This study aimed to evaluate the effect of incorporating hydrosoluble Opuntia ficus indica seeds extract in unpasteurized prickly pear juice and monitoring its stability. For this purpose, titratable acidity (TA), total soluble solids (TSS), browning index (BI), total phenolic compounds (TPC), total flavonoids (TF), antiradical activity (DPPH), ferric reducing antioxidant power (FRAP) and microbial analysis were monitored for both enriched and controlled juices during different time and temperature storage. Before storage, the enriched juice values were respectively 0.096±0.001%, 14.1±0.01%, 0.756±0.01, 133.3±3.4mgGAE/100ml, 5.58±0.07mgQE/100ml, 95.89±14.27mgGAE/100ml and 59.34±5.52mgGAE/100ml for TA, TSS, BI, TPC, TF, DPPH and FRAP; while 0.16±0%, 14.1 ±0.001%, 1.2±0.01, 88.39±4.2mgGAE/100ml, 3.98±1.003mgQE/100ml, 51.08±14.27 mgGAE/100ml and 50.33±5.16mgGAE/100ml for the control juice. The microbial analysis revealed the absence of microorganisms even the juices were unpasteurized. Moreover, the results revealed that the enrichment attenuated significantly the effect of storage; indeed, the use of the prickly pear seeds extract in combination with the juices can be a good alternative to enhance the shelf life of unpasteurized prickly pear juice, and improve their quality attributes as well as to minimize the unwanted changes in the nutritional and organoleptic properties.
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