The present study, it was aimed to determine the effect of antioxidants added to culture media on blastocyst development rates in in vitro embryo production. The material of the study consisted of oocytes collected from the ovaries taken from the slaughterhouse. Cumulus oocyte complexes (COC) were collected and classified under a stereomicroscope. Oocytes included in the study were subjected to maturation and fertilization stages. Probable zygotes were transferred to the culture (IVC) containing antioxidants (L-ergothionine 100 μM (n: 163), Vitamin E 100 μM (n: 151) Cysteamine 50 μM (n: 154) and were cultured in a tri gas incubator (Hera Cell- 6% O2%, 6%CO2, 88%N). Blastocyst rates and embryo quality were determined on the 6th and 7th days in culture medium. Differences in IVMFC stages were evaluated by chi-square test. 966 oocytes were collected from 162 ovaries collected from the slaughterhouse. It was determined that the number of oocytes per ovary was 5,96, and the number of A and B quality oocytes was 4.26. It was determined that 655 (94.93%) of a total of 690 oocytes undergoing in vitro maturation were mature. The cleavage rates of the groups were 83.44%; 80.79%; 79.87%, and 83.96%, respectively. 140 (21.37%) blastocysts were obtained from 655 oocytes taken into the culture stage and the blastocyst rates in the groups were 33.13%; 8.61%; 7.79%, and 32.62%, respectively. As a result of the study, it was determined that the rates of blastocysts in the L-ergothioneine added the group was similar to the control group, but the blastocyst rates decreased significantly in the cysteamine and Vitamin E added groups. It was thought that this decrease might have been affected by the dose of antioxidants or the adequacy of oocyte development

A Digital Object Identifier (DOI) não foi disponibilizada pela revista. | O objetivo deste trabalho foi avaliar o grau de associação entre caracteres fisicoquímicas do maracujazeiro-azedo (Passiflora edulis), auxiliando assim os programas de melhoramento que utilizam a seleção indireta. Avaliou-se 1120 frutos colhidos em ensaio com 26 famílias de irmãos completos de maracujazeiro-azedo, nos quais mensuraram-se as seguintes características: número de frutos por planta (NF), produção estimada (PE), massa média do fruto (MF), massa média da casca (MC), massa média da polpa (MP), comprimento médio do fruto (CF), diâmetro médio do fruto (DF), espessura média da casca (EC), teor médio de sólidos solúveis totais (SST), acidez total titulável média (ATT), e coloração da polpa (CP). A correlação entre número de frutos por planta com produção estimada por planta apresenta magnitude maior (rf = 0.92**) que a correlação massa do fruto com produção estimada (rf = 0.54**), indicando a maior contribuição do número de frutos por planta para obtenção de famílias mais produtivas. A correlação de maior magnitude foi comprimento do fruto com massa do fruto, indicando que a seleção de plantas com frutos pesados podem ser feitas diretamente no campo, a partir do comprimento do fruto. | The objective of this study was to evaluate the degree of association between characters of Passiflora edulis thus supporting the breeding programs that use indirect selection. Was evaluated in 1120 harvested in test 26 full-sib families of passion fruit, in which measured the following characteristics: number of fruits por plant (NF), estimated production (EP), fruit weight (FW) ; average mass of the shell (MC), average mass of pulp (MP), the fruit length (FL), average fruit diameter (FD), rind thickness (EC), the average content of soluble solids (TSS) , average total acidity (TTA), and flesh color (CP). The correlation between number of fruits por plant with an estimated production por plant has greater magnitude (rf = 0.92 **) that the correlation of fruit mass with an estimated production (rf = 0.54 **), indicating a greater contribution of the number of fruits por plant to obtain more productive families. The correlation of greater magnitude was the length of the fruit with fruit mass, indicating that the selection of plants with heavy fruit can be made directly in the field, from the length of the fruit.

The aim of the study was to investigate the effect of follicle size and follicle stimulating hormone (FSH) concentration on nuclear maturation of bovine oocytes in vitro. Follicles on bovine ovary were classified into 3 groups according to the diameter; small (

The objective of this study was to evaluate the degree of association between characters of Passiflora edulis thus supporting the breeding programs that use indirect selection. Was evaluated in 1120 harvested in test 26 full-sib families of passion fruit, in which measured the following characteristics: number of fruits por plant (NF), estimated production (EP), fruit weight (FW) ; average mass of the shell (MC), average mass of pulp (MP), the fruit length (FL), average fruit diameter (FD), rind thickness (EC), the average content of soluble solids (TSS) , average total acidity (TTA), and flesh color (CP). The correlation between number of fruits por plant with an estimated production por plant has greater magnitude (rf = 0.92 **) that the correlation of fruit mass with an estimated production (rf = 0.54 **), indicating a greater contribution of the number of fruits por plant to obtain more productive families. The correlation of greater magnitude was the length of the fruit with fruit mass, indicating that the selection of plants with heavy fruit can be made directly in the field, from the length of the fruit. | O objetivo deste trabalho foi avaliar o grau de associação entre caracteres fisicoquímicas do maracujazeiro-azedo (Passiflora edulis), auxiliando assim os programas de melhoramento que utilizam a seleção indireta. Avaliou-se 1120 frutos colhidos em ensaio com 26 famílias de irmãos completos de maracujazeiro-azedo, nos quais mensuraram-se as seguintes características: número de frutos por planta (NF), produção estimada (PE), massa média do fruto (MF), massa média da casca (MC), massa média da polpa (MP), comprimento médio do fruto (CF), diâmetro médio do fruto (DF), espessura média da casca (EC), teor médio de sólidos solúveis totais (SST), acidez total titulável média (ATT), e coloração da polpa (CP). A correlação entre número de frutos por planta com produção estimada por planta apresenta magnitude maior (r f = 0.92**) que a correlação massa do fruto com produção estimada (r f = 0.54**), indicando a maior contribuição do número de frutos por planta para obtenção de famílias mais produtivas. A correlação de maior magnitude foi comprimento do fruto com massa do fruto, indicando que a seleção de plantas com frutos pesados podem ser feitas diretamente no campo, a partir do comprimento do fruto.

In recent years the number of phytoplasma diseases that negatively affect yield and quality of many plants has been increasing around the world. As is known grafting, plant propagation materials (seed, bulb, tuber), parasitic plants and insect vectors play active role in the spread of phytoplasmas causing these diseases. These organasim which get through plate in the sieve tube element and systemically colonize the plant, it invade and multiply salivary glands, midgut, heomocel, brain and fat tissue after sucking up insect body with stylet. Subsequently they continue the cycle by moving to a new plant with a diseased insect feeding on a healthy plant. Although the existence of functional genomics in this cycle is known, there is little information about transmission of phytoplasma from vector to host plant or from host plant to vector, the mechanism of disease caused by phytoplasma in the plant and genomics involved in different reactions in the host plant against phytoplasmal infection.

Para establecer si la aplicación de ácido incrementa el efecto benéfico del desmane sobre las características del racimo de plátano Dominico Hartón, se llevó a cabo un experimento en la granja Montelindo (Palestina, Caldas). Quince días después de floración los racimos se dejaron con seis manos. El ácido giberélico, 1.000 mg L-1, se aplicó a los 15, 45 y 75 después de floración. Se empleó un diseño en bloques completos al azar con cuatro repeticiones, en un arreglo factorial de 2 x 2 x 3 (desmane, aplicación de la hormona, época de aplicación). El desmane mejoró significativamente el peso del racimo; la aplicación de ácido giberélico, incluso en racimos sin desmane, también mejoró significativamente la calidad de los frutos; la época de aplicación de la hormona causó aumentos altamente significativos en el peso del racimo de la primera mano y en el peso promedio del dedo; el mejor efecto sobre las características del racimo se logró con el desmane y la aplicación de la hormona a los 15 días después de la floración. | To establish whether the application of gibberellic acid increases the dehandling beneficial effect on bunch characteristics of the Dominico Harton plantain, an experiment was carried out at the 'Montelindo' farm (Palestina, Caldas). Fifteen days after flowering bunches were left with six hands. Gibberellic acid applications, 1000 mg L-1, were made at 15, 45 and 75 days after flowering. A complete randomized design with four replications was used in a factorial arrangement of the treatments of 2 x 2 x 3 (dehandling, hormone application, time of application). Dehandling significantly improves bunch weight; hormone application, even in bunches without dehandling, also significantly improves fruit quality; the time of hormone application caused highly significant differences in bunch weight, first hand weight and average finger weight; the best effect on bunch characteristics is obtained with the dehandling and hormone application 15 days after flowering.

A resposta das plantas à salinidade depende de fatores fisiológicos, bioquímicos e genéticos. Com este trabalho, objetivou-se estudar o impacto do tempo de aclimatação à solução nutritiva sobre a resposta do cajueiro à salinidade. Plantas de Anarcadium occidentale L., CCP06, cultivadas em vermiculita durante 28 dias, foram transferidas para solução nutritiva de Hoagland com 1/10 de sua força iônica, onde permaneceram por 1 e 7 dias. Ao final dos dois tempos, a solução nutritiva foi suplementada com 200 mM de NaCl, condições em que as plantas foram cultivadas por 0, 12, 24, 48 e 72 h. As plantas aclimatadas por 7 dias á solução de Hoagland apresentaram sinais fisiológicos mais compatíveis com a tolerância ao NaCl, como menor acumulação de Na+ e, também, menores danos nas membranas, peroxidação dos lipídeos, degradação de proteínas, acumulação de aminoácidos livres e acumulação de prolina. A amônia livre foi o melhor indicador da intensidade do estresse salino. Os danos de membranas aumentaram com a acumulação de Na+ nas folhas. Os resultados evidenciam que o tempo de aclimatação das raízes à solução nutritiva influencia a expressão de fatores capazes de atenuar os efeitos do estresse salino.<br>The plant response to salinity depends on physiological, biochemical and genetic factors and on their interaction with external environment. This work aimed to study the impact of adaptation time in nutrient solution on the cashew response to salinity. Seedlings of Anacardium occidentale L (CCP06) grown in vermiculite, for 28 days, were placed to grow in 1/10 ionic strength Hoagland solution for 1 and 7 days. At the end of each time the solution was supplemented with 200 mM NaCl, and under this condition the plants were monitored for 0, 12, 24, 48 and 72 h. The 7 days Hoagland solution acclimated plants had physiological index more compatible to tolerance to NaCl such as low Na+ accumulation and also low membrane damage, lipid peroxidation, protein degradation and free amino acids and proline accumulation. The free ammonia was the best index to predict the salt stress intensity. Membrane damage increased with Na+ accumulation in the leaves. The results evidence that the root adaptation time in Hoagland solution has influence on the expression of factors capable to alleviate the salt stress effects.