Affiner votre recherche
Résultats 391-400 de 517
Investigation of Genetic Diversity in Afghan Bread Wheat Genotypes Using SSR and AFLP Markers Texte intégral
2019
Mohammad Bahman Sadeqi | Said Dadshani | Mohammad Yousefi | Gul Mohammad Ajir
Genetic diversity assessment is the principle component for conservation and characterization of germplasm. Genetic diversity study of Afghan bread wheat genotypes is a first step to identify and to select high performance genotypes and distribute to wheat breeding programs. The main objective of this study is to investigate of genetic diversity in 35 Afghan bread wheat genotypes by using Simple Sequence Repeat (SSR) and Amplified Fragment Length Polymorphism (AFLP) markers. DNA extraction according to Cetyl Trimethyl Ammonium Bromide (CTAB) method was conducted and the total genomic DNA was isolated from each variety. Sixty-four SSR primer markers were used and eighteen EcoRI+(N)/MseI+(N) primer combinations with their primer sequences were used for selective polymerase chain reaction (PCR) amplification. Every SSR and AFLP fragment was scored as present (1) or absent (0) within all genotypes under study. Marker/ Value ratio of pairwise genetic distance between genotypes according to the SSRs data was from 0.508 to 0.691 with an average distance of 0.599. Relatively different grouping pattern in comparison to AFLP data observed through cluster analysis. Both types of molecular markers (AFLP and SSR) used in this research proved to be suitable for investigating genetic diversity in the genotypes of Afghan bread wheat, however, AFLP markers gave better view of genetically relationships among genotypes than the SSR markers. The grouping generated by AFLP data showed a special agreement with the origin regions of genotypes (Ariana-07 and Mazar-99 originating from the north of Afghanistan, Lalmi-03 and Kabul-02. Large number of DNA bands identified with AFLP markers might provide a better estimation of genetic similarity than those of SSR markers.
Afficher plus [+] Moins [-]Investigation of Anionic Detergent Pollution in Sarıkum Lake (Sinop) Texte intégral
2019
Zeynep Hasançavuşoğlu | Ayşe Gündoğdu
The study was aimed to determine the anionic detergent pollution level and some physico-chemical parameters (pH, salinity, dissolved oxygen, total dissolved substance, conductivity, temperature, phosphate, oxidation reduction potential) seasonally in Sarıkum Lake, which is located in Sinop province between November 2016 and July 2017. According to one-year measurement data, the highest of anionic detergent concentration was 0.056 mg / L and the lowest value was 0.021 mg / L. The classification of the results was made in accordance with the Water Pollution Control Regulation (2008) and Surface Water Quality Regulation (2016). In the study, Water quality is equivalent to Class I (high quality) in terms of anionic detergent, pH and temperature values but are equivalent to Class II (a bit dirty) and III (dirty) in point of dissolved oxygen, total dissolved matter and phosphate phosphorus values. In addition, it is equivalent to Class IV (very dirty) with regard to conductivity value. Detergent pollution coming from the settlements of Sarıkum Lake is observed to be low in contrast to expectations, while the high phosphorus value shows that the ecosystem living in the lake is exposed to agricultural pollution especially in terms of environmental resources.
Afficher plus [+] Moins [-]Correlation between the Agronomical Characteristics and Essential Oil Content of Origanum onites L. and Melissa officinalis L. Texte intégral
2019
Ayse Betül Avcı | Refika R Akcali Giachino
Due to their widespread use in the pharmaceutical, cosmetic and food industries, medicinal and aromatic plants have gained importance with an increasing number of studies being conducted concerning the yield and quality characteristics of these plants. In breeding studies, selection is successful when there a correlation is established between the essential oil content, composition, and morphological and agronomic characteristics. The aim of this study was to address the correlation between the yield and quality of medicinal and aromatic plants to present a different perspective for breeding studies. Therefore, in this study, the relationship among the characteristics and the essential oil content of Izmir oregano (Origanum onites L.) and lemon balm (Melissa officinalis L.) plants were investigated, and correlation and path analyses were performed. There was a significant negative correlation between the essential oil content and fresh herb (-0.279*), dry herb (-0.281*), and dry stem yield (-0.283*) of Izmir oregano. However, no significant correlation was found between the essential oil content and these agronomic characteristics in lemon balm. Fresh herb yield had a significant positive correlation with dry herb (0.661**), dry leaf (0.690**), and dry stem yield (0.610**). Dry herb yield was significantly and positively correlated with dry leaf (0.510*) and dry stem (0.461*) yields. A significant positive correlation was also observed between dry leaf and dry stem (0.688**) yields. According to the results of the path analysis, while the maximum negative effect on the essential oil content of Izmir oregano was provided by dry herb (-48.05%) yield, the highest direct positive effect belonged to dry leaf (21.69%) and dry stem (15.13%) yields. Dry leaf yield (-51.61%) and plant height (-45.71%) were the parameters that had the highest direct negative effect on the essential oil content of lemon balm while fresh herb (33.50%) and dry herb (30.27%) yields were the source of the highest direct positive effect.
Afficher plus [+] Moins [-]Ağ Kafeslerde Yetiştirilen Gökkuşağı Alabalığı Oncorhynchus mykiss (Walbaum, 1792)’in Kan Protein düzeyleri Texte intégral
2019
Asiye Başusta | Murat Dağ
Bu çalışmada Keban Baraj Gölü'nde ağ kafeslerde yetiştirilen gökkuşağı alabalığının kan protein düzeylerinin aylık değişimi incelendi. Bir yıl boyunca gökkuşağı alabalıklarından aynı yaş gurubundan her ay 10 örnek alınarak toplam boy ve canlı ağırlıkları ölçüldü. Kaudal venadan alınan kan örneklerinin serumu çıkarılarak; total protein, albumin, globulin ve fibrinojen miktarları belirlendi. İncelenen kan protein düzeylerinden; total protein (TP) 4,52 g/dl, albumin 2,37 g/dl, globulin 2,15 g/dl olarak yıl içerisinde en yüksek Mart ayında bulunmuştur. Fibrinojen ise en yüksek Ocak ayında (198,74 mg/dl) bulunmuştur. Toplam protein, albumin ve globulin düzeyleri en düşük kış aylarında bulunurken fibrinojen yaz aylarında en düşük miktarda bulunmuştur. İstatistiki analiz sonuçlarına göre tüm ölçümlerin aylık değişimleri önemli bulunmuştur. Albumin, globulin, fibrinojen değerleri ile toplam boy ve canlı ağırlık ve değerleri arasında pozitif bir ilişki bulunmuştur ve bu değerlerin aylık değişimleri önemli bulunmuştur.
Afficher plus [+] Moins [-]Toprak Kökenli Bitki Patojeni Funguslarla Mücadelede Biofumigasyonun Kullanım Olanakları Texte intégral
2019
Sevilay Saygı | Muharrem Türkkan | İsmail Erper
Fusarium, Rhizoctonia, Sclerotinia, Verticillium, Armillaria ve Rosellinia gibi toprak kökenli bitki patojeni funguslar, birçok bahçe ve tarla ürününde bitki ölümlerine ve verim kayıplarına sebep olan kök çürüklüğü hastalıklarına neden olmaktadır. Kök çürüklüğü hastalıklarının kontrolünde dayanıklı çeşit kullanımı, ürün rotasyonu, solarizasyon, fungisit uygulamaları ve toprak fumigasyonu gibi çeşitli mücadele yöntemleri uygulanmaktadır. Metil bromide ve kloropikrin gibi fumigantlarla yapılan toprak fumigasyonu toprak kökenli fungusların kontrolü için kullanılan en etkili yöntemlerden biridir. Ancak bu kimyasalların çevre ve insan sağlığına olumsuz etkilerinden dolayı dünyada ve ülkemizde kullanımı yasaklanmıştır. Bu nedenle, yeterli hastalık kontrolü sağlayan ve ticari fungisitlerle rekabet edebilen alternatif yöntemlerin araştırılmasına ihtiyaç vardır. Biofumigasyon kimyasal mücadeleye alternatif yöntemler arasında değerlendirilmekte nematodlar, yabancı otlar ve bazı fungusların neden olduğu hastalıkların mücadelesinde oldukça etkili olup, entegre mücadelenin bir parçası olarak kabul edilmektedir. Toprak kökenli fungusların kontrolünde örtücü bitkiler, organik gübre ve biyokatı atıklar biyofumigant olarak değerlendirilmektedir. Bu çalışmada toprak kökenli fungusların kontrolünde biyofumigasyon uygulamaları ve biyofumigantların funguslar üzerindeki etki şekilleri değerlendirilmiştir.
Afficher plus [+] Moins [-]Sivas İlinde Doğal Olarak Yetişen ve Yenilebilir Bir Bitki Olan Evelik (Rumex crispus) Bitkisinin Biyoaktivitesi ve Terapotik Nitelikleri Texte intégral
2019
Sevgi Durna Daştan | Hasan Durukan | Ahmet Demirbaş | Erol Dönmez
Bitkilerden elde edilen ekstrelerin birçoğunun biyolojik etkileri ve etki mekanizmaları hakkındaki bilimsel veriler hala yetersiz olmakla birlikte bu konuya olan ilgi her geçen gün artmaktadır. Bu çalışmada, Rumex crispus (Evelik) bitkisi mevsiminde Sivas yöresinden toplanarak kurutulmuş ve sonrasında yapraklı kısımlarından etanol, metanol ve su ekstraksiyonları elde edilmiştir. Çalışmanın amacı, elde edilen her bir ekstrenin bazı mikroorganizmalar üzerindeki antimikrobiyal aktivitelerinin değerlendirilmesi, toplam antioksidan seviyesi (TAS), toplam oksidan seviyesi (TOS), oksidatif stres indeksi (OSI) değerlerinin ve çeşitli hücre hatları üzerindeki anti-kanserojen niteliklerinin ortaya konulmasıdır. TAS, TOS ve OSI değerlerini tespit etmek için Rel Assay Diagnostics kitler kullanılmıştır. Bitki ekstrelerinin mikroorganizmalara karşı minimum inhibisyon konsantrasyonunu (MIC) belirlemek amacıyla mikrodilüsyon broth yöntemi kullanılmıştır. Çalışmada antimikrobiyal aktivite analizleri, Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Bacillus cereus (ATCC 11778), Klebsiella pneumonia (ATCC 13883), Candida albicans (ATCC 10231) ve Candida tropicalis (DSM 11953) mikroorganizmaları kullanılarak yapılmıştır. Normal fare fibroblast hücre hattı ve 3 farklı insan kanser hücre hattında MTT yöntemi ile bitki özütlerinin sitotoksik aktivitesi belirlenmiştir. Sonuç olarak, evelik bitkisinin etanol, metanol ve su ekstraklarının denendiği mikroorganizmalar üzerinde antimikrobiyal aktivitesinin bulunmadığı, yüksek düzeyde antioksidan aktiviteye sahip olduğu ve bazı hücre kültürlerinde sitotoksik aktivitesinin olduğu tespit edilmiştir.
Afficher plus [+] Moins [-]Effects of Housing Condition, Feeding Style and Age on Meat Colour, and Some Carcass Characteristics in Eastern Anatolian Red Bulls Texte intégral
2019
Sadrettin Yüksel | Alpay Karaçuhalılar | Fatma Yüksel | Burcuhan Balta
This study was conducted to evaluate the muscles of the brisket region of the Eastern Anatolian Red (EAR) bulls in different ages subjected to different housing conditions and feeding style, and to determine the relationships among carcass traits, meat colour parameters and carcass measurements. Totally 25 bulls between 15 and 24 m of age were allocated to five groups (G1,G2,G3,G4,G5) according to feeding type, housing condition and age. The bulls were weighed fourteen days apart, feed consumption measurements were taken every morning, and slaughtered after 130 days of fattening. The differences were significant among G1-G3 for pelvic fat, total groups for conformation, among G1,G2-G3,G4,G5 for marbling, among G1,G2,G3-G4,G5 for LD area, among G1-G3,G5 for degree of fatten, among G1,G5-G2 for fat thickness over (LD) Longissimus Dorsi. The differences were significant in terms of meat colour parameters such as lightness (L*) in G1,G2,G3-G4,G5 and hue value (H*) in G1-G2,G3,G4,G5. Also, the differences among the groups are significant in terms of carcass length and width of the round. The correlation coefficients of carcass characteristic, colour score and conformation are negative with lightness (L*) and yellowness (b*). Degree fatten had a positive correlation with marbling score. Conformation were positively correlated with carcass length. Carcass ribeye area and L* showed a more desirable result in the G4 and G5 groups compared to other groups. Assessing the accumulated data of carcass traits with carcass components including carcass measurement during the slaughter or later phases of slaughter, it may be possible to use as a marker for the determining beef quality in EAR bulls.
Afficher plus [+] Moins [-]Effects of Different Nutrient Solutions on Yield and Quality Parameters of Rocket Grown in Floating Water Culture Texte intégral
2019
Golgen Bahar Oztekin | Tuğba Uludağ | Yüksel Tüzel | Mahmut Tepecik
This study was conducted in styrofoam trays placed on an aerated nutrient solution in vats in order to determine the effect of different nutrient solutions on rocket plant growth, yield and quality. Seeds of Bengi F1 rocket cultivar were sown into peat as inserting each seed to each hole (17 ccs) of trays with 210 cells (957 plant m-2). Following germination in the germination chamber, seedling trays were moved to a climate controlled greenhouse for adaptation. After emergence, the seedlings were transferred to water culture. The nutrient solution was applied as “full dose” (mg/L: N 150, P 50, K 150, Ca 150, Mg 50, Fe 5.0, Mn 0.50, Zn 0.05, B 0.50, Cu 0.03, Mo 0.02), “half dose” (macro elements reduced by 50%) and “without nutrients” (water). Cultivation was performed in 2 consecutive periods and in each period 3 harvests were done and quality analysis was done on the leaves from the first harvest. Results were given as the mean of two periods. Results showed that plant growth, yield and quality parameters vary depending on the concentration of the nutrient solution. It was observed that as the concentration of nutrient solution decreased, plant growth, biomass, yield values decreased, leaf color and total chlorophyll concentration did not change and while nitrate content decreased, vitamin C content increased. Leaf N, P, K and Fe element contents decreased with half dose, whereas Ca and Mg content did not change. However, there was no statistical difference between full dose and half dose treatments in many measured parameters. When all the data obtained from the study are evaluated together; it is suggested that rocket can be grown in a floating water culture and the dose of macro elements as reduced 50% can be preferred because it reduces the nitrate content, increases the vitamin C content and allows the use of less fertilizers without causing any significant reduction in yield compared with full dose.
Afficher plus [+] Moins [-]Tohumluk Patates (Solanum tuberosum L.) Yumrularına Uçucu Yağ Uygulamalarının Sürgün ve Kök Gelişimine Etkileri Texte intégral
2019
Arif Şanlı | Yeşim Cirit | Bekir Tosun
Bu çalışma tohumluk patates yumrularına uygulanan bazı uçucu yağların patateste sürgün gücü ve kök gelişimine etkilerinin belirlenmesi amacıyla 2016 yılında yürütülmüştür. Çalışmada Agria çeşidine ait dormant durumdaki tohumluk yumrular farklı konsantrasyonlarda (150, 300, 450 ve 600 ppm) İzmir kekiği (Origanum onites L.), biberiye (Rosmarinus officinalis L.), dereotu (Anethum graveolens L.), kimyon (Cuminum cyminum L.), rezene (Foeniculum vulgare L.), adaçayı (Salvia officinalis L.) ve çörtük otu (Echinophora tenuifolia L. subsp. sibthorpiana (Guss.)) uçucu yağları ile muamele edilerek kontrollü şartlardaki tohum yataklarına dikilmiştir. Araştırmada dormansi süresi, sürgün uzunluğu, sürgün sayısı, sürgün çapı, sürgün ağırlığı, kök uzunluğu ve kök ağırlığı parametreleri incelenmiştir. Uçucu yağların sürgün gelişimine etkileri değişken olmuş adaçayı, biberiye, dereotu ve çörtük yağları sürgün gelişimini teşvik ederken, İzmir kekiği ve kimyon yağı uygulamaları kök gelişimi üzerine diğer uygulamalardan daha etkili olmuştur. Genel olarak yüksek dozda yapılan uygulamalar sürgün gelişimi olumsuz yönde etkilerken, kök gelişimini teşvik etmiştir. Çalışmada, tohumluk yumrulara dikim öncesi uçucu yağ uygulamalarının sürgün ve kök gelişimini olumlu yönde etkilediği ve bu uygulamaların arazi şartlarında yapılması ile daha sağlıklı ve güçlü bitki tesisi kurulabileceği sonucuna varılmıştır.
Afficher plus [+] Moins [-]Influence of Rice Husk Biochar on Water Holding Capacity of Soil in The Savannah Ecological Zone of Ghana Texte intégral
2019
Ammal Abukari
Soil moisture has a vital role in the cultivation of crops. Its sufficiency and availability to crop- water supplies depends on the management practices of soil and amount of available water to the soil. This study examines the influence of rice husk biochar on the water holding capacity of soil in the savannah ecological zone of Ghana. Increasing rate of rice husk biochar increased water-holding capacity of soils. 4t/ha rice husk biochar showed higher significant differences among the treatments. It is recommended that 4t/ha rice husk biochar should be applied to increase the water-holding capacity of the soil.
Afficher plus [+] Moins [-]