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Physicochemical Investigation of Some Oil Emulsions Oxidized by UV-B Radiation
2022
Temel Kan Bakır | Hüseyin Kastrati
In this study, the effects of UV B (50 µW/cm2) radiation at 306 nm on oil-in-water emulsions (O/W) using canola oil, soybean oil and linoleic acid were investigated. The oxidation rates of emulsions incubated at pH 7.0 and 37°C in the presence and absence of Cu (II) ions were determined by using iron (III) thiocyanate and thiobarbituric acid methods for the determination of primary and secondary products, respectively. The UV B-induced oxidation rates followed the order LA / Cu (II) > LA > Canola Oil / Cu (II) > Canola Oil > Soybean Oil / Cu (II) > Soybean Oil for both crops. Simultaneously, structural studies were performed using gas chromatography mass spectrometry (GC-MS). It was found that 18-carbon polyunsaturated fatty acid (PUFA) and monounsaturated fatty acid (MUFA) contents have an important role in canola and soybean oil oxidation. The unsaturated fatty acid contents of small carbon numbers increased for each emulsion sample, while the oxidation of fatty acid changes did not follow a regular order.
Afficher plus [+] Moins [-]In Vitro of Melaleuca viridiflora Sol. ex Gaertn Plant Investigation of Antimicrobial, Anticancer and Cytotoxic Activities
2022
Eda Sönmez Gürer | Tutku Tunç
In this study; it was aimed to investigate the anticancer and cytotoxic activities on DU-145 prostate cancer and MCF-7 breast cancer cell lines and WI-38 human fibroblast cell line of essential oil (Nioli) obtained from Melaleuca viridiflora Sol. ex Gaertn. plant and its antimicrobial effects on various bacteria and yeast cells. Content analyzes of Nioli essential oil were made by Gas Chromatography-Mass Spectrometry (GK-KS). The antimicrobial effects of the essential oil were determined using Disk Diffusion and Minimum Inhibition Concentration (MIC) tests. Its cytotoxic effects were determined by the XTT (2,3-bis-(2-methoxy-4-) nitro-5)-sulfophenyl)-2H-tetrazolium-5-carboxanilide) test; were investigated at different concentrations in breast cancer (MCF-7), prostate cancer (DU-145) and healthy human fibroblast (WI-38) cell lines. In the disc diffusion method of essential oil; It was determined that it formed an inhibition zone against Klebsiella pneumoniae bacteria at 200, 100, 50, 25 and 12.5 µg/mL concentrations, and against Candida albicans yeast at 200 and 100 µg/mL concentrations. Nioli essential oil showed the highest antibacterial effect against Bacillus cereus. While it was moderately effective against Pseudomonas aeruginosa and Candida albicans, it reached MIC values effective against other bacteria. It was observed that the anticancer activity of Nioli essential oil was more effective in DU-145 prostate cancer cells compared to MCF-7 breast cancer cells, and it had no toxicity in WI-38 healthy human fibroblast cells.
Afficher plus [+] Moins [-]Exploring Zinc and Boron Chemo-Priming Effects on Low-Vigour Seed Germination and Seedling Establishment of Sunflower (Helianthus annuus L.)
2022
Negar Ebrahim Pour Mokhtari | Ferhat Kızılgeçi | Raees Ahmed | Muhammad Aamir Iqbal
Poor germination and suboptimal seedling growth constitute as prime factors in lowering the achene yield and seed oil content of sunflower (Helianthus annuus L.), while chemo-priming with micronutrients might serve as a biological viable strategy provided source and dose optimization are performed. A trial was executed to appraise the comparative impact of seed priming with different doses of micronutrients like boron (B) and zinc (Zn) on sunflower germination and seedling growth traits of sunflower. The experiment was comprised of six treatments of B (0, 0.2, 0.4, 0.8, 1.6, 3.20 ppm) and Zn (0, 4, 8, 12, 16 and 20 mM) each, while seed germination, root and seedling growth related traits were taken as experimental variables. The results revealed that all treatments remained ineffective regarding seed germination and seed vigour of sunflower, while B and Zn doses of 0.2 ppm and 12 mM respectively, enhanced seedling emergence rate and vigour. The Zn (8 mM) significantly improved root length along with their fresh and dry weights, while all doses of B imparted antagonistic effects on root attributes. Likewise, Zn (8 mM) remained superior for shoot length, fresh and dry weights, while higher doses of B remained contra-productive for shoot growth of sunflower.
Afficher plus [+] Moins [-]Free Radical Scavenging and Antinociceptive Activities of the Aqueous Extract from Matricaria chamomilla L. Flowers.
2022
Chahrazed Kaoudone | Fatima Benchikh | Cherif Abdennour | Hassiba Benabdallah | Walid Mamache | Smain Amira
Background: Matricaria chamomilla L. (M. chamomilla) is a famous medicinal plant distributed worldwide. It is widely used in traditional medicine to treat all kinds of diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders. It is also used as a sedative, antispasmodic, antiseptic, and antiemetic. Our aims in this study was thus to quantify the phenolic, flavonoids contents in the flower of this plant, and also to evaluate the in vitro antioxidant potential and the in vivo analgesic activity. Methods: The total phenolic and flavonoid contents of the plant aqueous extract (MCAqE) were estimated using the Folin–Ciocalteu and AlCl3 colorimetric methods, respectively. However, DPPH method was used to evaluate the in vitro antioxidant activity. Analgesic activity was tested by acetic acid induced writhing model in mice. Results: Quantitative determination of total polyphenols and flavonoids revealed that this extract contained 158.41±1.6 mg gallic acid equivalent/g of dry extract and 37.06±0.56 mg quercetin equivalent/g of dry extract, respectively. The antioxidant activity of the plant extract was important (IC50=3.08±0.25 mg/mL). MCAqE extract, at 400 mg/kg, showed analgesic activity (39.60±8.70%) against acetic acid induced pain in mice while the standard reference drug Diclofenac sodium exhibited 90.44±2.80% activity at 10 mg/kg dose.
Afficher plus [+] Moins [-]Evaluation of The Spirulina (Arthrospira platensis Gomont) Antimicrobial Activity
2022
Amor Bencheikh | Walid Mamache | Asma Gharzouli | Ahlem Kouachi | Haif Khadidja | Meriem Barkahoum Daichi | Noureddine Rouag
The present work was carried out to enhance biomass production of Spirulina (Arthrospira platensis Gomont) in a modified medium and to investigate “in vitro” its ability to produce antimicrobial substances against pathogenic bacteria and phytopathogenic fungi. The antibacterial activity was evaluated by the solid medium diffusion method on two pathogenic bacteria (Bacillus cereus & Klebsiella sp.), And the antifungal activity was evaluated on three phytopathogenic fungi (Alternaria solani, Cladosporium sp., Fusarium culmorum). The antibacterial test showed that the aqueous extract produced by S. platensis was more active against Gram-positive than on Gram-negative bacteria, the highest antibacterial activity was recorded against Bacillus cereus. Moreover, the Antifungal test showed that the aqueous extract was active against all three tested fungi and the highest antifungal activity was recorded against F. culmorum with over 90% inhibition of mycelial growth. The results of this research proved that cyanobacteria could be a good source for the production of antimicrobial agents which could be effective when compared with contemporary antimicrobial compounds and it can be used in the Biocontrol of several plant fungal diseases.
Afficher plus [+] Moins [-]Thermal Degradation and Thermodynamic Properties of Ascorbic Acid, Total Phenolic Content and Antioxidant Activity of Convective Dried Kiwi Fruits
2022
Fadime Begüm Tepe
In the current study, thermal degradation and thermodynamic properties of ascorbic acid, total phenolic content, and antioxidant activity of convective dried kiwi fruits were investigated. To determine kinetic model describing thermal degradation of these parameters, zero order, first order, fractional conversion and Weibull model were used. Weibull model gave the best fitting to thermal degradation of these parameters. Moreover, the rate constant of the thermal degradation reaction increased with the increment in drying temperatures, meaning that these degradation reactions were temperature dependent. On the other hand, total phenolic content had the lowest activation energy and ∆H value indicating the lowest thermal stability in comparison to others. The degradation reactions endothermically occurred during drying process. Additionally, it was seen that the reason for the higher degradation rate at higher temperatures as the absolute values of ∆S increased. It is important to evaluate thermal degradation of bioactive compounds for the design of the thermal processes. This study will be beneficial for the convective drying process design due to these results.
Afficher plus [+] Moins [-]Determination of The Yield and Yield Parameters of Demre Sivrisi, Dogal Sarı Sivri and Dogal Carliston (Capsicum annuumm L.) Pepper Varieties Growed By Fertigation Method in Greenhouse Conditions
2022
Ahmet Demirbaş | Hasan Durukan | Mustafa Öztük | Handan Saraç
Demre Sivri, Dogal Sarı Sivri and Dogal Carliston pepper (Capsicum annuum L.) species were used as test plants in the study. The research was carried out in a total of 9 plots with 3 replications according to the randomized blocks experimental design. Seeds were made into seedlings by regularly watering for about 60 days in the prepared peat and perlite mixture (1:1 V/V) in viols and planting was carried out in the greenhouse environment when they had 3-4 leaves. Irrigation processes in the research were carried out by drip irrigation method, and irrigation was carried out every 3 days during the 20 days period from planting to 7 leaves of pepper plants. In other processes, irrigation was done every 2 days until the plants were harvested. Considering the results of soil analysis in the research, fertilization was done by fertigation method (fertilization with drip irrigation) for pepper purely at 20 kg N da-1, 6 kg P2O5 da-1, 20 kg K2O da-1. In the study, nitrogen (N) ammonium sulfate (NH4)2SO4, phosphorus (P) MKP (monopotassiumphosphate) and potassium (K) potassium nitrate (KNO3) forms were applied. N, P, K, calcium (Ca), magnesium (Mg), iron (Fe), zinc (Zn), manganese (Mn) and copper (Cu) analyzes were made in the leaf samples taken when the pepper plant reached half of the fruit size and yield were determined. According to the results of the research, Dogal Carliston stood out in the first year in terms of yield (2987 kg da-1), while Demre Sivri (2788 kg da-1) gave the highest yield in the second year.
Afficher plus [+] Moins [-]Production of Vitamin B12 by Propionic Acid Bacteria and Investigation of Effective Parameters
2022
Hayriye Göknur Ağca Küçükaydın | Göksel Tırpancı Sivri | Ömer Öksüz
Propionic acid bacteria (PAB) are extremely important because of the metabolites they produce (vitamins, propionic acid, conjugated linoleic acid, and bioactive peptides). Nowadays, the increasing interest in healthy nutrition has led to the formation of the concepts of probiotics, postbiotics and functionality with studies. Vitamin B12 is one of the postbiotics produced by PAB and is known to be beneficial for human health. Due to its coenzyme feature, it plays a key role in the body process, as well as shows anti-inflammatory properties. The recommended dietary intake of vitamin B12 for adults is 4 μg/day. Although it is highly found in meat products, it is known that the bioavailability of B12 in dairy products is higher. Fermented dairy products, especially products that contain PAB in their natural microflora, are good tools for the production and intake of vitamin B12. In this study, the factors affecting the microbial production of vitamin B12 were examined.
Afficher plus [+] Moins [-]Mathematical Modeling of Fresh Green Pepper (Capsicum L.) Dried at Different Powers in Microwave Oven
2022
Fatma Coşkun Topuz
In this research, fresh green peppers were dried in a kitchen microwave oven at 600W, 700W and 800W. The drying curves of the study were compared with 5 different thin layer drying models in the literature. The changes in the moisture values of the samples against time were expressed graphically. R2, χ2 and RMSE values were used to determine the most suitable model for dried green peppers. Color values of fresh and dried peppers were determined. It was found that the L* and b* values of the dried peppers were lower, and the a* value was higher than the fresh green peppers. In addition, the rehydration rate of dried peppers was calculated. It was determined that the rehydration ability of the peppers decreased as the applied microwave power increased. For this reason, it was found that the peppers with the highest rehydration rate were those dried with 600W. Also, it was found that the most suitable model for all microwave powers among the five drying models was the Logarithmic drying model. It was calculated that the R2 values of the drying models ranged between 0.830-0.999, χ2 values between 0.0001- 0.4684 and RMSE values between 0.0014-0.6121. It was determined that the highest R2 (0.997-0.999), the lowest χ2 (0.0001-0.0002) values, and the lowest RMSE (0.0014-0.0035) values for all microwave powers belong to the Logarithmic drying model.
Afficher plus [+] Moins [-]Özlüce Baraj Gölü’nde Yaşayan Üç Luciobarbus Türünün (Luciobarbus barbulus, Luciobarbus esocinus ve Luciobarbus xanthopterus) Metrik ve Meristik Özelliklerinin Belirlenmesi
2022
Mustafa Düşükcan | Mücahit Eroğlu | Mehmet Zülfü Çoban
Bu çalışmada Özlüce Baraj Gölü’nde yaşayan Luciobarbus barbulus (Heckel 1847), Luciobarbus esocinus Heckel 1843 ve Luciobarbus xanthopterus Heckel 1843 türlerine ait 51 morfometrik ve 8 meristik karakter özellikleri incelenmiştir. Morfometrik karakterlerin ve birbirine oranlarının maksimum, minimum, ortalama, standart sapma, standart hata ve değişim katsayıları hesaplanmıştır. Ayrıca, morfometrik ölçümlerin standart boy oranlarına ait regresyon denklemi ile korelasyon katsayıları hesaplanmış olup bunun sonucunda pozitif yönde kuvvetli ilişkiler olduğu belirlenmiştir. Meristik karakterlerden, yanal çizgi ve transversal yöndeki pul sayıları, yüzgeçlerdeki basit ve yumuşak ışın sayıları, solungaç diken sayıları, farinks diş sıra ve sayıları ortaya konarak her türe ait tanımlayıcı özellikler belirlenmiştir.
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