Fermentation is the oldest traditional method in order to protect against spoilage and pathogenic microorganisms. Thermal treatment, pH and water activity lowering and preservative addition other food preservation techniques that are commonly used. Although, as preservatives, many improved antibiotic and chemical agents have been gained, there are some other factors such as rapid resistance to antibiotics used in bacteria, in order to limit their use in food, to be found dimensions of threatening human health of the chemical protection and to cause allergic reactions. Recently, studies on bacteriocins that are produced by the safe bacteria, such as lactic acid bacteria, contain no toxic and adverse effects for human consumption have gained sudden intensity in accordance with the request against consumers in developing microbiologically safe and minimal processed food. There are some important effects using of bacteriocins accepted the new generation of antimicrobial agents as food preservatives such as extension of shelf life, reducing of the use of chemical preservatives and the economic loss caused degradation by microorganisms. Nisin, defined as GRAS (Generally Recognized as Safe) by FDA and named the E234 code 'Nisin-protective' or 'natural protective' located in the food additives list, first used in 1988 as a natural preservative by prohibiting of antibiotics using in food in the US. Nowadays in more than 50 countries, there have been more successful implementation of the nisin protected up the food from milk and dairy products to canned foods many food products

The second decade of the XXI century has experienced the development of meat substitutes. The origin of this niche is said to emerge from environmental concerns. One of the market leaders in this infant industry is Beyond Meat company. The study aims to identify the impact of the capital markets and media coverage for an infant industry, such as meat alternatives, on the example of Beyond Meat. A sizeable portion of the investment in this new branch of the food sector came from the capital markets, which expect a return on their investment. The Beyond Meat case suggests that these substantial investments did not bring expected returns, which several factors, such as the post-COVID bear market and lack of demand for meat substitute products, could have caused. Thus, despite awareness campaigns, so far, the company has experienced losses. The development of this market niche acted contrary to what was expected. The market failings correlate with the loss of interest of the retail investors, which was attempted to be measured by this research. As a proxy for the retail investor interest, Google Trends Index was used and compared to the stock prices (a method often described as a Buzz Indicator).

ATAK-S is a newly developed native hybrid layer. Although the laying performance of this hybrid has been studied and determined, the performance response of the hybrid to different environmental temperature conditions is not known. This study was therefore undertaken to determine the effect of cold and heat stress on egg quality traits. Hens were divided into three different groups; control (20°C), low (12°C) and high (32°C). A total of 360 hens, with 120 in each of the groups, were used in the study. Hens were held in three tier battery cages in an environmentally controlled poultry house. The study lasted for 3 weeks. No differences were found among different groups in terms of shape index, albumen height and Haugh unit of the egg quality traits. It was found that the differences among the heat groups in terms of egg shell breaking strength, egg weight, shell thickness and yolk colour were significant and the value of these traits decreased under the heat stress conditions, whereas they were not affected from the cold stress.

Passiflora with approx. 575 species is the most diverse genus in Passifloraceae, with Colombia and Ecuador holding the largest number of taxa. It is divided into five subgenera, one of them being Astrophea, a group of arborescent and woody species. The aim of this research, was to assess the diversity and conservation status of the species in the subgenus Astrophea native to Colombia, and investigate their current and potential distribution. Subsequently, national and international herbarium collections and scientific literature were reviewed. This research was further supplemented by recent field collections. The results were analyzed with Excel © and maps obtained by Diva-GIS © to which the IUCN criteria were applied for each species. 24 species were identified, distributed within 28 departments, by assessing 375 records. The potential distribution ranges of the species, suggests niches associated to the Amazonía and Orinoquía, bioregions with large gaps in collection, provided they are in the species preferred altitudinal ranges (0-500 m). The most diverse parts of the country are the center west and northeast, with more than 7 taxa per area. 46% of species have some degree of threat and 37.5% are endemic. Despite this, 80% of species occur outside protected areas, which further complicate its conservation status according to the IUCN criteria. Eleven species are newly reported for the 14 departments. Collection efforts should be focused on areas with optimal bioclimatic conditions for the growth of species as regions for potential distribution. | Passiflora con ca. 575 especies es el género más rico de Passifloraceae cuyo centro de diversidad está en Colombia y Ecuador. Éste se divide en cinco subgéneros, entre estos Astrophea agrupa las especies arborescentes y leñosas. El propósito de la investigación fue conocer la diversidad y estado de conservación de especies del subgénero Astrophea para Colombia, sumando aspectos de distribución actual y potencial. Por lo anterior, se revisaron colecciones de herbarios nacionales e internacionales y literatura científica, complementando con colectas de campo. Los resultados fueron analizados con Excel© y los mapas obtenidos mediante Diva-GIS©, además los criterios de la IUCN se aplicaron para cada especie. Se registraron 375 observaciones, agrupadas en 24 especies y distribuidas en 28 departamentos, a lo largo de los valles interandinos del río Cauca y Magdalena. La distribución potencial, sugiere nichos asociados a las regiones de Amazonía y Orinoquía; bioregiones con amplios vacíos de colecta ubicadas dentro de las preferencias de distribución por rango altitudinal de las especies (0-500 m.s.n.m.). Las áreas con mayor riqueza son el centro occidente y nororiente del país con más de siete especies por zona. Pese a esto, el 80% de las especies están por fuera de áreas protegidas, lo que se agrava bajo los criterios de la IUCN, pues el 46% presentan algún grado de amenaza, además del alto porcentaje de endemismo (37,5%). Once especies son incluidas como nuevos reportes para 14 departamentos. Esfuerzos de colecta deben orientarse en las regiones de distribución potencial que poseen condiciones bioclimáticas óptimas para el desarrollo de las especies. | Passiflora con ca. 575 especies es el género más rico de Passifloraceae cuyo centro de diversidad está en Colombia y Ecuador. Éste se divide en cinco subgéneros, entre estos Astrophea agrupa las especies arborescentes y leñosas. El propósito de la investigación fue conocer la diversidad y estado de conservación de especies del subgénero Astrophea para Colombia, sumando aspectos de distribución actual y potencial. Por lo anterior, se revisaron colecciones de herbarios nacionales e internacionales y literatura científica, complementando con colectas de campo. Los resultados fueron analizados con Excel© y los mapas obtenidos mediante Diva-GIS©, además los criterios de la IUCN se aplicaron para cada especie. Se registraron 375 observaciones, agrupadas en 24 especies y distribuidas en 28 departamentos, a lo largo de los valles interandinos del río Cauca y Magdalena. La distribución potencial, sugiere nichos asociados a las regiones de Amazonía y Orinoquía; bioregiones con amplios vacíos de colecta ubicadas dentro de las preferencias de distribución por rango altitudinal de las especies (0-500 m.s.n.m.). Las áreas con mayor riqueza son el centro occidente y nororiente del país con más de siete especies por zona. Pese a esto, el 80% de las especies están por fuera de áreas protegidas, lo que se agrava bajo los criterios de la IUCN, pues el 46% presentan algún grado de amenaza, además del alto porcentaje de endemismo (37,5%). Once especies son incluidas como nuevos reportes para 14 departamentos. Esfuerzos de colecta deben orientarse en las regiones de distribución potencial que poseen condiciones bioclimáticas óptimas para el desarrollo de las especies.

The aim of this study was to determine the effect of thermal manipulations during early and late embryogenesis on asymmetry in terms of sides of shank length, shank width and face length of broilers. Incubation conditions were 37.5°C and 55% relative humidity for control group throughout the incubation period until the 19th days. In the thermally treated eggs during early embryogenesis (8-10 days), incubation temperature was increased to 41°C and relative humidity to 65% for 3 hours (12.00-15.00) on the 8th-10th days of incubation. Also, in the late embryogenesis stage (16-18 days) incubation temperature was increased to 41°C and relative humidity to 65 % for 3 hours (12.00-15.00) on the 16th-18th days of incubation. Total 16 chickens were selected at randomly from all experimental groups to determine the asymmetry. The weekly left and right sides of shank length, shank width and face length of chickens were measured from 7 days of age to 35 days of age, and relative asymmetry values were calculated. There was no significant difference among the groups in point of relative asymmetry. Asymmetry values were reduced due to aging.

En el Laboratorio de Fitopatología de la Universidad de Caldas, Colombia, se evaluó el efecto de las con-centraciones 0.5, 1, 2, 4, 6, 8, 16, 32, 64, 96 y 240 ppm de la molécula MCW-2 sobre la mortalidad de poblaciones de nematodos de los géneros Radopholus, Pratylenchus, Meloidogyne y Helicotylenchus du-rante 24, 48 y 72 h de exposición. Como testigo relativo se empleó Carbofuran y como testigo absoluto se utilizó agua. Se empleo un diseño al azar en un arreglo factorial de dos productos, 12 concentraciones y tres tiempos de exposición. Los resultados indicaron que, los niveles de mortalidad en las concentracio-nes superiores a 16 ppm fueron mayores con MCW-2 que con Carbofuran; en todas las concentraciones la mortalidad aumentó a medida que el tiempo de exposición fue mayor; Meloidogyne fue el género más sen-sible a MCW-2, mientras que Pratylenchus lo fue a Carbofuran; la concentración letal media de MCW-2 para los cuatro géneros de nematodos fue < de 125 ppm y la de Carbofuran varió entre 415 y 715 ppm. Se concluye que MCW-2 constituye una buena opción para el control de nematodos en plátano, aunque es necesario evaluarlo en plántulas en condiciones de campo.

To establish the effectiveness of a molecule with nematicida action against parasitic nematode of the plantain, experiments were conducted at the Phytopathology laboratory of the University of Caldas. The effect of concentrations of 0.0, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0, 16.0, 32.0, 64.0, 96.0 y 240.0 ppm of MCW-2 was evaluated on the mortality of populations of nematodes of the genera Radopholus, Pratylenchus, Meloidogyne y Helicotylenchus during 24, 48 and 72 h of exposition. As the relative control Carbofuran was used. The absolute control was the treatment with water. A randomized experimental design was used in a factorial arrangement of two products, 12 concentrations and three times of exposure. The levels of mortality at concentrations above 16 ppm were higher with MCW-2 than with Carbofuran and vice versa; that in all of the concentrations mortality increased with the time of exposition; that Meloidogyne was the genus most sensitive to MCW-2 while Pratylenchus so was to Carbofuran and that the 50% lethal concentration for MCW-2 for the four genera of nematodes was below 115 ppm and to Carbofuran was between 415 and 715 ppm. It was concluded that MCW-2 constitutes a good option to the control of nematodes in plantain, although it necessary to evaluate it on plants growing in plastic bags. | En el Laboratorio de Fitopatología de la Universidad de Caldas, Colombia, se evaluó el efecto de las concentraciones 0.5, 1, 2, 4, 6, 8, 16, 32, 64, 96 y 240 ppm de la molécula MCW-2 sobre la mortalidad de poblaciones de nematodos de los géneros Radopholus, Pratylenchus, Meloidogyne y Helicotylenchus durante 24, 48 y 72 h de exposición. Como testigo relativo se empleó Carbofurán y como testigo absoluto se utilizó agua. Se uso un diseño al azar, en un arreglo factorial de dos productos, doce concentraciones y tres tiempos de exposición. Los resultados indicaron que los niveles de mortalidad en las concentraciones superiores a 16 ppm fueron mayores con MCW-2 que con Carbofurán; en todas las concentraciones la mortalidad aumentó a medida que el tiempo de exposición fue mayor; Meloidogyne fue el género más sensible a MCW-2, mientras que Pratylenchus lo fue a Carbofurán; la concentración letal media de MCW-2 para los cuatro géneros de nematodos fue < de 125 ppm y la de Carbofurán varió entre 415 y 715 ppm. Se concluye que MCW-2 constituye una buena opción para el control de nematodos en plátano, aunque es necesario evaluarlo en plántulas en condiciones de campo.

In this study, the effect of using gamma irradiated barley and wheat 0, 10 and 100 kiloGray (kGy) and enzyme supplementation on the egg product criterions and external and internal egg quality was examined. In this experiment, 36 weeks old, 324 Brown Nick laying hens were used for ten week. The study was a randomized complete block design with 9 dietary treatments within 6 blocks of 54 pens each 6 birds per pen. Although egg weight was not significantly affected by gamma irradiation; enzyme supplementation of barley based diet significantly increased egg weight compared to barley control group. As egg mass production was considered, enzyme and 100 kGy gamma irradiation significantly improved the egg mass production compared to barley control. The feed conversion ratio was significantly improved by using gamma irradiated barley and enzyme in barley based diets (P

Calla (Zantedeschia aethiopica (L.) K. Spreng), is a cut flower which had obtained importance in recent years, but postharvest handling is limited. Thus, in order to know the fluorescein concentration effect and pH of pulse solution on inflorescences calla postharvest, were evaluated in Teotitlan de Flores Magon, Oaxaca-Mexico, four fluorescein concentrations (0, 20, 40 and 60 %, respectively) and two pH levels (acid and alkaline) in a completely randomized design with factorial arrangement, having a total of 24 experimental units, which was established by vase life and calla inflorescence with white spathe color, immersed in a fluorescein solution adjusted as appropriate at acidic or alkaline pH, where response variables were as follows: vase life, water consumption, stem diameter, absorption fluorescein stem base and fluorescein vertical absorption by floral scape. Results indicates the increased vase life is achieved at alkaline pH by fluorescein addition of 20, 40 and 60 %, respectively. Likewise, the higher water consumption is achieved at this pH. From this research, we can conclude fluorescein can affect the calla physiology for increasing vase life. | Calla (Zantedeschia aethiopica (L.) K. Spreng), is a cut flower which had obtained importance in recent years, but postharvest handling is limited. Thus, in order to know the fluorescein concentration effect and pH of pulse solution on inflorescences calla postharvest, were evaluated in Teotitlan de Flores Magon, Oaxaca-Mexico, four fluorescein concentrations (0, 20, 40 and 60 %, respectively) and two pH levels (acid and alkaline) in a completely randomized design with factorial arrangement, having a total of 24 experimental units, which was established by vase life and calla inflorescence with white spathe color, immersed in a fluorescein solution adjusted as appropriate at acidic or alkaline pH, where response variables were as follows: vase life, water consumption, stem diameter, absorption fluorescein stem base and fluorescein vertical absorption by floral scape. Results indicates the increased vase life is achieved at alkaline pH by fluorescein addition of 20, 40 and 60 %, respectively. Likewise, the higher water consumption is achieved at this pH. From this research, we can conclude fluorescein can affect the calla physiology for increasing vase life.

The aim of the study was to investigate the effect of follicle size and follicle stimulating hormone (FSH) concentration on nuclear maturation of bovine oocytes in vitro. Follicles on bovine ovary were classified into 3 groups according to the diameter; small (

A Digital Object Identifier (DOI) não foi disponibilizada pela revista. | O objetivo deste trabalho foi avaliar o grau de associação entre caracteres fisicoquímicas do maracujazeiro-azedo (Passiflora edulis), auxiliando assim os programas de melhoramento que utilizam a seleção indireta. Avaliou-se 1120 frutos colhidos em ensaio com 26 famílias de irmãos completos de maracujazeiro-azedo, nos quais mensuraram-se as seguintes características: número de frutos por planta (NF), produção estimada (PE), massa média do fruto (MF), massa média da casca (MC), massa média da polpa (MP), comprimento médio do fruto (CF), diâmetro médio do fruto (DF), espessura média da casca (EC), teor médio de sólidos solúveis totais (SST), acidez total titulável média (ATT), e coloração da polpa (CP). A correlação entre número de frutos por planta com produção estimada por planta apresenta magnitude maior (rf = 0.92**) que a correlação massa do fruto com produção estimada (rf = 0.54**), indicando a maior contribuição do número de frutos por planta para obtenção de famílias mais produtivas. A correlação de maior magnitude foi comprimento do fruto com massa do fruto, indicando que a seleção de plantas com frutos pesados podem ser feitas diretamente no campo, a partir do comprimento do fruto. | The objective of this study was to evaluate the degree of association between characters of Passiflora edulis thus supporting the breeding programs that use indirect selection. Was evaluated in 1120 harvested in test 26 full-sib families of passion fruit, in which measured the following characteristics: number of fruits por plant (NF), estimated production (EP), fruit weight (FW) ; average mass of the shell (MC), average mass of pulp (MP), the fruit length (FL), average fruit diameter (FD), rind thickness (EC), the average content of soluble solids (TSS) , average total acidity (TTA), and flesh color (CP). The correlation between number of fruits por plant with an estimated production por plant has greater magnitude (rf = 0.92 **) that the correlation of fruit mass with an estimated production (rf = 0.54 **), indicating a greater contribution of the number of fruits por plant to obtain more productive families. The correlation of greater magnitude was the length of the fruit with fruit mass, indicating that the selection of plants with heavy fruit can be made directly in the field, from the length of the fruit.

The objective of this study was to evaluate the degree of association between characters of Passiflora edulis thus supporting the breeding programs that use indirect selection. Was evaluated in 1120 harvested in test 26 full-sib families of passion fruit, in which measured the following characteristics: number of fruits por plant (NF), estimated production (EP), fruit weight (FW) ; average mass of the shell (MC), average mass of pulp (MP), the fruit length (FL), average fruit diameter (FD), rind thickness (EC), the average content of soluble solids (TSS) , average total acidity (TTA), and flesh color (CP). The correlation between number of fruits por plant with an estimated production por plant has greater magnitude (rf = 0.92 **) that the correlation of fruit mass with an estimated production (rf = 0.54 **), indicating a greater contribution of the number of fruits por plant to obtain more productive families. The correlation of greater magnitude was the length of the fruit with fruit mass, indicating that the selection of plants with heavy fruit can be made directly in the field, from the length of the fruit. | O objetivo deste trabalho foi avaliar o grau de associação entre caracteres fisicoquímicas do maracujazeiro-azedo (Passiflora edulis), auxiliando assim os programas de melhoramento que utilizam a seleção indireta. Avaliou-se 1120 frutos colhidos em ensaio com 26 famílias de irmãos completos de maracujazeiro-azedo, nos quais mensuraram-se as seguintes características: número de frutos por planta (NF), produção estimada (PE), massa média do fruto (MF), massa média da casca (MC), massa média da polpa (MP), comprimento médio do fruto (CF), diâmetro médio do fruto (DF), espessura média da casca (EC), teor médio de sólidos solúveis totais (SST), acidez total titulável média (ATT), e coloração da polpa (CP). A correlação entre número de frutos por planta com produção estimada por planta apresenta magnitude maior (r f = 0.92**) que a correlação massa do fruto com produção estimada (r f = 0.54**), indicando a maior contribuição do número de frutos por planta para obtenção de famílias mais produtivas. A correlação de maior magnitude foi comprimento do fruto com massa do fruto, indicando que a seleção de plantas com frutos pesados podem ser feitas diretamente no campo, a partir do comprimento do fruto.

The present study, it was aimed to determine the effect of antioxidants added to culture media on blastocyst development rates in in vitro embryo production. The material of the study consisted of oocytes collected from the ovaries taken from the slaughterhouse. Cumulus oocyte complexes (COC) were collected and classified under a stereomicroscope. Oocytes included in the study were subjected to maturation and fertilization stages. Probable zygotes were transferred to the culture (IVC) containing antioxidants (L-ergothionine 100 μM (n: 163), Vitamin E 100 μM (n: 151) Cysteamine 50 μM (n: 154) and were cultured in a tri gas incubator (Hera Cell- 6% O2%, 6%CO2, 88%N). Blastocyst rates and embryo quality were determined on the 6th and 7th days in culture medium. Differences in IVMFC stages were evaluated by chi-square test. 966 oocytes were collected from 162 ovaries collected from the slaughterhouse. It was determined that the number of oocytes per ovary was 5,96, and the number of A and B quality oocytes was 4.26. It was determined that 655 (94.93%) of a total of 690 oocytes undergoing in vitro maturation were mature. The cleavage rates of the groups were 83.44%; 80.79%; 79.87%, and 83.96%, respectively. 140 (21.37%) blastocysts were obtained from 655 oocytes taken into the culture stage and the blastocyst rates in the groups were 33.13%; 8.61%; 7.79%, and 32.62%, respectively. As a result of the study, it was determined that the rates of blastocysts in the L-ergothioneine added the group was similar to the control group, but the blastocyst rates decreased significantly in the cysteamine and Vitamin E added groups. It was thought that this decrease might have been affected by the dose of antioxidants or the adequacy of oocyte development