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Effect of Polyamine on Pigmentation, Reactive Oxidative Species and Antioxidant under Drought in Maize (Zea mays L.)
2018
Sharmin Akter | Md. Golam Rasul | Mohammad Zakaria | Md. Mahathir Sarker | Irin Sultana Nila | Sudipta Dutta | Md. Masudul Haque | Md. Motiar Rohman
To examine polyamines (PAs) effect in modulating the drought induced by polyethylene glycol (PEG) in maize (Zea mays L.) seedlings (variety Khoibhutta, 8 day seedlings grown in petri dish in incubator) were subjected to 20% PEG (polyethylene glycol) followed by 20 µmol PAs, Putrescine (Put), Spermidine (Spd) and Spermine (Spm) with PEG solution for 48 hours. Sharp decrease in Relative Water Content (RWC), Chl a, Chl b, carotenoid (Car) and total pigment content was observed under drought compared to control condition, while PAs application reversed their decreasing trends. PEG significantly increased Reactive Oxidative Species (ROS) [superoxide (O2•−) and H2O2], Methyl Glyoxal (MG), Melondialdehyde (MDA) and Lipoxigenase (LOX) activity, while Pas decreased the contents considerably (except MG) as compared to those under drought. Drought increased proline content, which was further augmented in PA treatments. PAs failed to incline glyoxalase’s (Gly-I and Gly-II) activities, reduced under PEG. The activity and western blot confirmed the accumulation of Glutathione S-Transferase (GST) under drought, but PAs failed to augment the activity. Ascorbic Acid (AsA) and Glutathione (GSH) got oxidized into Dehydroascorbate (DHA) and oxidized Glutathione (GSSG) under drought but PAs effectively maintained homeostasis. Superoxide Dismutase (SOD), Peroxidase (POD), Ascorbate Peroxidase (APX), Glutathione Peroxidase (GPX), Monodehydroascorbatereductase (MDHAR), Dehydroascorbatereductase (DHAR), and Glutathione Reductase (GR) inclined in drought stressed seedlings, while Catalase (CAT) activity decreased under drought. PAs addition increased SOD, POD, GPX, CAT, MDHAR, and GR activities, but declined DHAR activity. These findings suggested important role of PAs in increasing tolerance under short term drought by modulating antioxidant effect.
Afficher plus [+] Moins [-]Türkiye’de Üretilen Organik ve Konvansiyonel Sütlerin Bazı Fizikokimyasal Özellikleri ile Yağ Asitleri Kompozisyonu ve Antioksidan Kapasitesinin Belirlenmesi
2018
Bayram Ürkek | Mustafa Şengül
Bu çalışmada Türkiye’de üretilen konvansiyonel ve organik çiğ sütlerin bazı fizikokimyasal özellikleri, yağ asitleri kompozisyonu ve antioksidan kapasitesi üzerine çiftlik üretim tipinin (konvansiyonel ve organik) ve süt toplama zamanın etkisinin ortaya konulması amaçlanmıştır. Araştırmada organik ve konvansiyonel çiftliklerden bir yıl boyunca iki ayda bir 9’ar adet çiğ süt örnekleri toplanmıştır. Organik ve konvansiyonel olarak üretilen sütlerin yağ asidi kompozisyonu, antioksidan kapasitesi, toplam fenolik madde ve bazı fizikokimyasal özellikleri (kurumadde, yağ, protein, kül, titrasyon asitliği (% laktik asit), pH, özgül ağırlık) incelenmiştir. Araştırma sonuçlarına göre konvansiyonel ve organik süt örneklerinin ortalama kurumadde, yağ, protein, kül, özgül ağırlık, asitlik ve pH değerleri sırayısla %12,06-11,97, %3,67-3,50, %3,33-3,34, %0,67-0,66, 1,0381-1,0381 g mL-1, %0,18-0,16 6,67-6,73 olarak belirlenmiştir. Konjuge linoleik asit oranlarının organik sütlerde %1,39 ile %2,87 arasında, konvansiyonel sütlerde %1,67 ile %2,96 arasında değiştiği belirlenmiştir. Sonuç olarak, süt bileşenlerini (kurumadde, yağ, protein ve kül), yağ asidi kompozisyonunu, EC50 ve toplam fenolik madde değerlerinin çiftlik üretim tipinden etkilenmediği ortaya konulmuştur. Diğer taraftan süt yağı, protein, yağ asidi oranları (oleik asit hariç), EC50, inhibisyon ve toplam fenolik madde değerlerinin toplama zamanına bağlı olarak önemli değişimler gösterdiği belirlenmiştir.
Afficher plus [+] Moins [-]Consortium Application of Endophytic Bacteria and Fungi Improves Grain Yield and Physiological Attributes in Advanced Lines of Bread Wheat
2018
Ghulam Muhae-Ud-Din | Muhammad Amjad Ali | Muhammad Naveed | Khalid Naveed | Amjad Abbas | Javed Anwar | Muhammad Hammad Tanveer
Increasing human population places pressure on agriculture. To feed this population, two time increase in the current wheat production is needed. Today agriculture is becoming input intensive with more reliance on synthetic fertilizers and agrochemicals to fulfil the feed demand of the growing numbers. Use of synthetic fertilizer since last few years is impacting the soil quality. In this scenario, the use of beneficial endophytic microbes is an attractive strategy to overcome the use of synthetic products. To investigate the effect of consortium application of endophytic bacteria and fungus on plant growth, grain yield moisture status, a pot experiment was conducted in different wheat lines. It comprised four treatments like control, application of bacterial strain Bacillus sp. MN54, fungal strain Trichoderma sp. MN6, and their consortium (Bacillus sp. MN54 + Trichoderma sp. MN6). The effect of consortium application was more prominent and significantly different from the sole application of bacteria and fungus. The results showed that with a consortium application of endophytic bacteria and fungus, there was 28.6, 4.3, -6.3 and -3.7% increases in flag leaf area, chlorophyll content, relative membrane permeability and water content respectively. Consortia of endophytic microbes also resulted in the yield enhancement through the betterment of various yield attributes like number of spikelet’s, grains per spike and grain yield per plant (32.2, 25.8 and 30.8%, respectively). So, consortia of endophytic microbes can greatly promote the progress of plants in dry land agriculture and increase the yield in an environmentally sustainable way.
Afficher plus [+] Moins [-]Analysis of Polymorphisms on GH-MspI and IGF1-SnaBI Loci in Five Turkish Native Cattle Breeds
2018
Yasemin Öner | Onur Yılmaz | Candan Eriş | Nezih Ata | Cihan Ünal | Seyrani Koncagül
Growth Hormone (GH) and Insulin like Growth Factor-I (IGF1) are members of somototrophine axis pathway. They play a role in key on several mechanisms such as postnatal growth, cell differentiation and metabolism. Due to their vital importance, polymorphisms on the genes coding are worth to be understood. In this study five native cattle breeds (Native Southern Yellow (NSY), South Anatolian Red (SAR), Anatolian Grey (AG), Native Black (NB), East Anatolian Red (EAR) were investigated by PCR-RFLP method for GH-MspI and IGF1-SnaBI loci. 198 and 194 samples were analyzed for GH-MspI and IGF1-SnaBI loci, respectively. In both two loci two alleles and three genotypes were observed. Predominant alleles were A and B for GH-MspI locus IGF1-SnaBI loci, respectively. Frequencies of A and B alleles were calculated between 0.400-0.875 and 0.846-0.903 for GH-MspI locus IGF1-SnaBI loci, respectively. While among investigated population only EAR population was at Hardy-Weinberg equilibrium for IGF1-SnaBI locus, for GH-MspI only, in SAR population no deviation from Hardy-Weinberg equilibrium.
Afficher plus [+] Moins [-]Effect of Cassava Whey on the Physicochemical Parameters and Heavy Metals Distribution in Soil
2018
Segun Michael Abegunde | Simeon Ajibade Akinyele | Isaac Olatunde Awonyemi
The processes involved in the conversion of cassava tuber into various products generate large volumes of wastes in solid, liquid and gaseous forms. These wastes when discharged into the environment have serious environmental impacts on the natural composition and structure of soil. The aim of this research work was to investigate the effects of cassava whey on the physicochemical properties and metal contents of soil samples around Gari facrory at Erinfun Village along Federal Polytechnic road, Ado-Ekiti. The physicochemical parameters determined were the pH, moisture content, loss on ignition, organic matter, water holding capacity, bulk density, particle density, total porosity, calcium (Ca), magnesium (Mg) and cyanide (CN). Heavy metals determined were lead (Pb), zinc (Zn), copper (Cu), manganese (Mn), cadmium (Cd), chromium (Cr) and iron (Fe). The presence of cassava whey in the soil led to increasing soil acidity, moisture content, water holding capacity, CN, Cr, Ni, Pb and As concentrations while reductions were observed in total porosity, organic matter, loss on ignition, particle density, Ca, Mg, Fe, Mn and Zn concentrations. The results show appreciable variations of physicochemical parameters and heavy metal contents when compared to the control sample.
Afficher plus [+] Moins [-]Bakteriyel Yollarla Metal Nanopartiküllerin Sentezi
2018
Fikriye Alev Akçay | Ayşe Avcı
Nanoteknolojik yöntemlerle nano boyuta indirgenen metal partiküller temelde biyomedikal ve fizikokimya olmak üzere eczacılık, elektrik-elektronik, otomotiv sanayi, gıda sanayi gibi pek çok farklı alanda karşımıza çıkmaktadır. Fiziksel, kimyasal ve biyolojik olmak üzere üç farklı üretim yöntemi bulunan nanopartiküller ağırlıklı olarak kimyasal yöntemlerle üretilmektedir. Ancak fiziksel ve kimyasal yöntemler yüksek sıcaklık, yüksek basınç ve toksik kimyasalların kullanımını gerektirdiği için çevre dostu ve ekonomik değildir. Bu nedenle yeşil teknoloji olarak da adlandırılan, çevre dostu ve sürdürülebilir bir yaklaşım olan biyolojik yöntemlerle metal nanopartiküllerin üretimine olan ilgi son yıllarda artmıştır. Bazı bitki ekstraktlarıyla ve mikroorganizmaların hücre içi ve hücre dışı salgılarıyla birtakım indirgenme reaksiyonları gerçekleşmekte ve metal nanopartikülleri üretilmektedir. Bakteriler doğadaki çeşitlilikleri, kolay izole edilebilir olmaları ve nanopartikül üretimindeki kolaylıkları sebebiyle son yıllarda etkin birer nano fabrika görevi görmektedirler. Bu makalede, metal nanopartiküllerin bakteriyel yöntemlerle üretimleri ve uygulama alanları derlenmiştir.
Afficher plus [+] Moins [-]An Investigation of Consumers' Consciousness Level About Food Safety in Honey Consumption: The Case of Niğde Province
2018
Betül Gürer | Ethem Akyol
Recently, food safety which has become one of the decisive factor in demand for all food products, is becoming a major concern due to increasing sensitivity of consumers to food production and consumption. As honey is produced and marketed on more uncontrolled conditions, which is relatively different from other agricultural products, the food safety issue is becoming more intense. In this study, it is examined what kind of applications are applied in food safety in bee products in Turkey. In the second part of the study, it is aimed to determine the importance of food safety in honey consumption of urban consumers in Niğde province based on the primary data and to put consumer's consciousness in this subject. For this purpose, it was conducted a survey by interviewing face to face with 286 consumers. In the study, besides basic statistical methods, univariate analysis of variance and factor analysis method were also used to determine the effective factors in purchasing according to income groups. As a result of the research, it was determined that there is a great lack of confidence in the quality of honey in purchasing from the market due to both the concern of sugar added fraudulent honey and residual problem by consumers. For this reason, consumers prefer to buy honey from directly producers rather than market. In addition, consumers are more likely to believe that honey is less safety than in previous years. Health consciousness is an important factor in consumers purchasing honey and health and quality consciousness develops positively especially as the income level of consumers’ increases.
Afficher plus [+] Moins [-]Effect of Growth Hormone Genetic Polymorphism on Calf Birth Weight
2018
Zeynep Sönmez | Memiş Özdemir | Vecihi Aksakal
The aim of study were to present relationships between birth weight of 94 Holstein calves with genotype structures of each sample’s Growth Hormone (GH) determined through PCR-RFLP method and to detect the population’s genetic variation by determining the genotype and allel frequency distribution. In the study, according to the analysis result, it was determined that Growth Hormone gene LL genotype was 41.9 kg, LL was 40.8 kg, and VV was 42.2 kg for the birth weight. A significant relationship between birth weight with GH genotypes could not be found. It was seen that the calf population is not stable according to Hardy-Weinberg genetic balance test.
Afficher plus [+] Moins [-]Determination of Chemical, Nutritional and Fermentation Properties of Citrus Pulp Silages
2018
Selma Büyükkılıç Beyzi | İsmail Ülger | Mahmut Kaliber | Yusuf Konca
This study was carried out to investigate the possibilities of making silage from fruit juice industry waste.For this purpose, orange, lemon and tangerine pulp silage quality have been determined by comparing silage with maize and beet pulp silage.Treatment groups; 1) orange, 2) tangerine, 3) lemon, 4) maize and 5) sugar beet pulp. The silages were evaluated after 2 months from ensiling in the following areas: subjective evaluation, pH, dry matter, organic matter, crude protein, acid detergent fiber, neutral detergent fiber, ether extract and energy values (metabolizable energy) and net energy for lactation were calculated. As a result, it was determined that fruit juice industry residues were lower in terms of dry matter, but they contained higher energy due to their high organic matter content, digestibility and low cellulose content. In addition, it was determined that citrus pulp was evaluated as silage without any contribution.
Afficher plus [+] Moins [-]Botanical Composition and In Situ Dry Matter Degradability of Legume- Grass Mixture Pasture Fertilized with Different Amounts of Nitrogen
2018
Numan Kılıçalp | Mustafa Avcı | Hatice Hızlı | Rüştü Hatipoğlu | Tugay Ayaşan
This study was conducted to determine the effects of different nitrogen (N) fertilizer levels on botanical composition, dry matter (DM) yield, chemical composition, in situ ruminal DM and NDF degradability and net energy lactation (NEL) of a native legume-grass mixture pasture. Field and degradability trials were arranged in a Complete Randomized Block Design with three replicates. Pasture was fertilized at six levels of N fertilizer (ammonium nitrate, N0, N50, N100, N150, N200 and N250 kg/ha). Three rumen cannulated Holstein Friesian heifers (3-4 year old) were used. Feed samples were incubated for 0, 8, 12, 24, 36, 48, 72 and 96 h. Application of nitrogen fertilizer results indicated that nitrogen fertilizer decreased the legumes ratio (LR) of pasture, approximately 22.4%, Although N fertilizer increased the grass ratio (GR) of pasture approximately 55.8%. Dry matter (DM), Net energy lactation (NEL, Mcal/kg) and ash contents of pasture were increased by increasing N fertilizer level. However, when N fertilizer level increased acid detergent fiber (ADF) concentration of pasture decreased. Application of the different rate of nitrogen fertilizer had no effect on in situ rumen degradability of DM and NDF of pasture. It was also found that there was the significant positive relationship between effective NDF degradability at 48 h. rumen incubation period and different level of N fertilizer. In addition, it was determined that there was a linear and quadratic positive relationship between DM yield and different nitrogen doses. In conclusion, different rates of nitrogen application changed botanical composition, decreased ADF content and increased NEL and effective rumen degradability of neutral detergent fiber (EDNDF 48 h.) of pasture.
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