The tomato plant exhibiting leaf rolling, witches’ broom, distorted and elongated flower’s sepals in Iğdır province, Turkey, was observed. Total DNA extraction was performed from the symtomatic fresh tomato sample. All DNAs were subjected to Direct and Nested polymerase chain reaction (PCR) with universal primer sets that amplified the 16S rRNA of phytoplasmas. PCR products were purified from agarose gel and cloned into the pGEM T-Easy cloning vector. Recombinant plasmids were introduced into the prokaryotic cloning bacteria by electroporation. Plasmid isolation was performed by selecting one of the positive clones randomly and sequencing was performed by Next Generation Sequencing (NGS). Sequencing results revealed that the 16S rRNA gene associated with phytoplasma was 1251 nucleotides in size, and this sequence was denominated as ‘Iğdır 10’ isolates and recorded in the GenBank under the MT344968 accession number. The virtual restriction fragment length polymorphism (V-RFLP) and phylogenetic analysis of the 16S rDNA sequence confirmed that the cause of disease in infected tomato plants was 'Candidatus Phytoplasma trifolii' ('Ca. P. trifolii') (16SrVI-A, Clover proliferation group), with a 1.00 similarity coefficient. This present study is the first report of ‘Ca. P. trifolii’ and its nucleotide sequence analysis in naturally infected tomato in Iğdır province.

The primary purpose of this study is whether gender, which is a culturally and socially defined concept, affects the perception of landscape architecture. Secondly, it is aimed to determine the preferences of the designs created in landscape architecture studies according to gender and to guide the questionnaire results in the creation of areas suitable for user requests during the design phase. The concept of admiration, preference and accompanying beauty, the effect it has on people according to gender, show various differences. These differences lead to perceptual selectivity in terms of colour, texture, form, functional and aesthetically depending on gender. Therefore, tastes and preferences are not expected to be a single judgment but to vary. Since there is no possibility of face-to-face surveys due to the current Covid-19 outbreak, online surveys were conducted on 220 people who can be reached in professional groups on social media. In the content of the questionnaire, the landscape design criteria and design elements were handled, and the visuals and preferences selected for each definition were directed to the participants as questions. According to the evaluated results, differences occurred in perceptual preferences depending on gender. In addition to the fact that there was no statistically significant difference between the preferences of women and men in terms of design criteria and elements, the preferences of both groups were combined in natural or close to nature forms. Considering the proportions, there was no obvious difference in design perceptions preferred by gender.

Plants are important antioxidant sources. In our study, total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of endemic Helleborus vesicarius Aucher ex Boiss.were determined. The aerial parts of the plant samples were dried and extracted with ethanol (EtOH). TAS and TOS values of plant extract were determined using Rel Assay Diagnostics kits. As a result of the studies, the TAS value of H. vesicarius was determined 5.548±0.23, the TOS value was 13.778±0.119 and the OSI value was 0.249±0.009. In this context, the plant has been shown to have significant antioxidant potential.

Sous-vide process is a cooking method for vacuumed products which are placed in a water bath or steam oven at controllable low temperatures and specific long times. This technique is widely used for cooking of meat and meat products which have limited shelf life throughout cold storage. Temperature, time and vacuum parameters used in sous-vide method are effective factors on meat quality. It is realised that meat tenderness increases with this technique due to cooking is performed at low temperatures. For the same reason, more water is retained in the texture and cooking losses are reduced. In addition to this, it is appeared that the sous-vide method provides protection of nutrient components that are water-soluble and/or adversely affected by high temperature applications, so that this method increases the nutritional value of meat. On the other hand, use of controllable cooking temperatures facilitates to reach targeted core temperatures in meat. With the help of homogeneous distribution of heat, even colour formation on meat can be observed. Oxidative reactions in the product are limited by the application of vacuum, so that product quality can be preserved for a long time. The risk of food safety as a result of low temperature applications in sous-vide cooking is eliminated by increasing cooking times. In this review, it is aimed to inform about effects of sous-vide cooking technique on meat quality under the heads of; texture, juiciness and cooking losses, colour, flavour, lipid and protein oxidation and microbial quality. Besides, the effects of sous-vide cooking method on quality parameters, different effects of sous-vide and traditional cooking methods on quality parameters were deeply discussed.

Shiitake mushroom (Lentinula edodes (Berk.) Pegler) popular as both edible and medicinal, is one of the most cultivated and consumed mushroom species in the world. Cultivation of this mushroom on different agricultural wastes has been experimented in many studies until today. Nevertheless, agricultural waste trials are going on as agricultural production patterns of the countries are different. In this study, volatile aroma composition of shiitake mushroom produced on different agricultural wastes (wheat stalk, wheat bran, peanut shell, corn cob and vine pruning waste) was compared. Oak sawdust was selected as control because of its common use in literature and substrate materials were mixed at different ratios. In addition, some substrate mixtures were prepared with poplar sawdust. Volatile compounds of the shiitake mushroom were determined by using headspace-solid phase microextraction (HS-SPME) technique combined with Gas Chromatography-Mass Spectrometer (GC-MS). At the end of study, 41 volatile compounds were detected and dimethyl trisulfide, benzaldehyde, dimethyl disulfide, 1-octen-3-ol and 3-octanone were found to be dominant volatile components. Flavour and fragrance of mushrooms are dependent on many volatile aroma compounds and their proportions. While the concentration of eight-carbon components and hydrocarbons was found the highest by using wood straw, amount of sulphur containing compounds and alcohols increased by agricultural wastes in this study. In addition, corn waste positively affected aldehyde compounds.

The objectivity of this study was to investigate some blood minerals and parameters in Honamli goats reared under semi-intensive conditions. 90 goats (2-4 ages) in different three herds kept under similar manage mental conditions were chosen as research materials in Teke Border of Antalya Province. Serum Ca 6.786±0.206 mg/dL, P 4.094±0.173 mg/dL, Cl 111.105±0.582 mmol/L, K 4.519±0.066 mmol/L, Mg 2.161±0.05 mg/dL, Na 148.047±0.508 mmol/L, Fe 110.706±2.510 μg/dL, UIBC 155.025±4.333 μg/dL and Tp 7.055±0.120 g/dL were determined by Roche Diagnostics, Cobas 8000 modular analyser series, immunoassay. After then, total iron- binding capacity (TIBC), transferrin (Tf), transferrin saturation (TSAT) and Ca++ levels were calculated from each other using different biochemical formulas. Ratios between the some minerals were calculated and presented in the text. Also, very high significant differences (P

The present study was carried out to evaluate the effect of nitrogen on growth and yield of chili (Capsicum annuum L.) under rooftop conditions. The single factorial experiments were laid out in Randomized Complete Block Design (RCBD) with three replications. Four levels of nitrogen N0: 0 kg N ha-1, N1: 90 kg N ha-1, N2: 110 kg N ha-1 and N3: 130 kg N ha-1 were used in this experiment. Growth and yield contributing parameters significantly influenced by different doses of nitrogenous fertilizers. The dose of N2 gave the highest plant height (80.97 cm) and most of the morphological parameters increased with increasing nitrogen levels up to N2. The treatment N3 gave the highest fruit length (4.93 cm), yield of fruits plant-1 (165.33 gm), average fruit yield plot-1 (0.66 kg), individual fruit weight (1.97 g) and average fruit yield (5.533 kgha-1). Based on the present results, it can be suggested that use of 130 kg N ha-1 increased plant growth and fruit yield of chili in rooftop garden.

Essential oil composition, antimicrobial activity and flavonoid contents of leaf-fruits of Cedrus libani and Pinus nigra subsp. pallasiana were determined with GC-MS, disc diffusion method and HPLC in three different period. When the essential oil composition of leaf and fruits of P. nigra ssp pallasiana collected in April, July and September, major components were α-pinene, β-pinene, limonene, β-caryophyllene ve germacrene-D. On the other hand, additionally to these contents, myrcene and α–terpineol were also detected in C. libani. According to HPLC analysis, rutin, quercetin, kaempferol, naringin and resveratrol flavonoids were detected in different proportions. While rutin (154.33 µg g-) and resveratrol (20.02 µg g-) has the highest ratio in C. libani, quercetin (9.65 µg g-) and naringin (9.31 µg g-) were detected in P. nigra subsp. pallasiana along with rutin (39.66 µg g-). According to the antimicrobial activity results the essential oils of C. libani obtained in April has produced higher activity than that of July and September. On the contrary, the essential oils from P. nigra subsp. pallasina have produced the best antimicrobial activity on September compared to April and July. As a result, C. libani and P. nigra subsp. pallasiana essential oils have a composition showing antimicrobial activity and their harvesting season should be determined for the best and effective content.

The aim of this study was to examine the effect of five different wavelengths of light on the comfrey plant (Symphytum officinale) (family Boraginaceae). The light source and wavelengths used in the study were UV-A (390-410 nm), blue (465-485 nm), red (620-630 nm) and cool white (CW) daylight (400-700 nm, 6500 K), LED (Light Emitting Diode). In the study, each of the 5 different light applications was applied for 45 days (T1: 100% blue; T2: 100% red; T3: 60% blue + 35% red + 5% UV-A; T4: 100% CW daylight; T5: 80% CW + 20% red). The experiments were carried out under conditions of 22C temperature, 60% humidity, 16/8 hours light/dark and 180 µmol.m-2.s-1 Photosynthetically Active Radiation (PAR). After each application, measurements were taken of number of leaves, number of roots, height of plant, amount of chlorophyll in leaves, leaf colour and brightness. According to data obtained, the different wavelengths of the coloured light applied in the growing environment created a change in colour and brightness of the leaves, height of the plant, length of the roots, and number of leaves and roots.

Honey is a popular product consumed for its health benefits. It is an effective antimicrobial an antioxidant agent. Globally, palynological and chemical methods are among the means of authenticating honey quality, geographical origin and floral origin. Six honey samples from six Nigerian towns (Abi, Ikom, Lokpanta, Nsukka, Okigwe and Shaki) were subjected to the aforementioned tests. Eighty-six pollen taxa were recorded in all the samples. The richest sample with seventy-three taxa was from Nsukka, followed successively by Okigwe, Lokpanta, Shaki, Ikom and Abi samples with sixty-eight, sixty-seven, sixty-two, fifty-nine and fifty-seven pollen species respectively. The oil palm Elaeis guineensis pollen dominated the samples in different proportions except Shaki honey dominated by Acacia spp., The commonest plant family was Fabaceae (Caesalpinioideae, Mimosoideae, Papilionideae) with twenty-one taxa followed by Euphorbiaceae, Combretaceae, with four representatives and Rubiaceae with three taxa each. The physico-chemical analysis carried out were total moisture, total ash content, colour assessment, percentage of total solids, relative density, acidity, and Fischer’s Test. The samples were found to concur with the international standards for honey.