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Research on Possible Effects of Acrylamide and Vitamin E Administered to Pregnant Rats on Placenta Tissue
2017
Mehmet Erman Erdemli | Eyüp Altınöz | Zeynep Aksungur | Zümrüt Doğan | Harika Gözükara Bağ | Yusuf Türköz
Investigate the changes that occur in the placenta tissues of pregnant rats that were administered acrylamide (AA) and vitamin E as a protective agent during pregnancy. Thirty rats that were proven positive for pregnancy with vaginal smear test were randomly distributed into control, corn oil, vitamin E, acrylamide and vitamin E + acrylamide groups. Pregnant rats were decapitated on the 20th day of the experiment. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAS), total oxidant capacity (TOS) and Xanthine oxidase (XO) levels were measured in placenta tissues. It was determined that acrylamide application during pregnancy statistically significantly increased MDA, TOS and XO levels and reduced GSH and TAS levels in the placenta tissue of pregnant rats when compared to all other groups, and GAS and TAS levels statistically significantly increased in vitamin E administered group when compared to all other groups and TOS and XO levels were decreased to control group levels. It was observed that orally administered AA changed the antioxidant / oxidant equilibrium favoring the oxidants by increasing MDA, XO and TOS levels in pregnant rats and caused oxidative stress, while vitamin E administration returned the antioxidant / oxidant equilibrium back to normal levels, preventing oxidative stress induced toxicity.
Afficher plus [+] Moins [-]Tuz Stresinin Nane (Mentha piperita L.)’de Büyüme ile Uçucu Yağ Miktarı ve Bileşenleri Üzerine Etkileri
2017
Nilgün Göktürk Baydar | Özkan Çoban
Peppermint is one of the valuable medicinal and aromatic plants with its powerful flavour and fragrance. Its leaf and essential oil are used in cosmeceuticals, foods, pharmaceutical and personal care products. It is well known that yield and secondary metabolite biosynthesis in the medicinal and aromatic plants are strongly influenced by abiotic stresses. Salinity is also one of the most important stress factors affecting plants. This study was carried out to determine the effect of salt stress on growth, essential oil content and oil constituents in peppermint plants. For this aim, three different concentrations of sodium chloride NaCl (0, 100 and 150 mM) were applied to peppermint plants. As a result of the study it was determined that all of the investigated parameters were significantly affected by NaCl applications. Shoot length, fresh and dry weights of plants and essential oil content decreased in line with the elevating level of NaCl. Besides, NaCl also caused differences in essential oil composition. Menthol and menthone, the main components of the peppermint oil, reduced significantly when NaCl concentration rose to 150 mM. Based on the results, it may be concluded that NaCl especially at high concentrations considerably and negatively affected plant growth, essential oil content and quality of the essential oil.
Afficher plus [+] Moins [-]Organik Asitler ve Su Ürünleri Yetiştiriciliğinde Kullanımı
2017
Ferbal Özkan Yılmaz | Arzu Özlüer Hunt
Organik asitlerin su ürünleri yetiştiriciliğinde, fonksiyonel yem katkı maddeleri olarak kullanılmasının öngörülebilir gelecekte belirgin bir şekilde artacağı düşünülmektedir. Dünya su ürünleri yetiştiriciliğinin sürekli genişlemesi, büyük bir pazar potansiyeli oluşturmaktadır. Bununla birlikte küresel ısınmanın bir sonucu olarak, su ürünleri yetiştiriciliğinde hastalık salgınlarında artma tehlikesi, önemli bir problem olarak ortaya çıkabilecektir. Birçok ülkede antibiyotik kullanımına ilişkin yasaklar veya kısıtlama uygulamaları bulunmaktadır. Su ürünleri yetiştiriciliğinde, antibiyotiklere karşı uygulanabilir bir alternatif olarak organik asitlerin kullanımının uygunluğunu, yapılan çalışmalar ile ortaya konulmaktadır. Organik asitler, antimikrobiyal özelliklerinden dolayı zararlı bakterilerin çoğalmasını engelleyerek, hayvanların gastrointestinal sisteminde yararlı bakterilerin büyümesini teşvik ederek, hayvanların sağlığını iyileştirme özelliklerini göstermektedirler. Yapılan çalışmalar, organik asitlerin fonksiyonel yem katkı maddeleri olarak, su ürünleri yetiştiriciliği endüstrisinde sürdürülebilir bir sağlık yönetimi programının bir parçası olarak kullanılabilirliğini ortaya koymaktadır.
Afficher plus [+] Moins [-]Determination of Spatiotemporal Variations in Heavy Metal Concentration through Orontes River
2017
Ece Kılıç | Mehmet Fatih Can
Water quality in surface waters is an important concern since it directly affects public health. Therefore, to provide adequate quality of water, monitoring studies have been conducted all around the world. In this study, multivariate statistical methods were used to understand the spatial and temporal changes in heavy metal concentration (including As, Cd, Cr, Cu, Fe, Hg, Mn, Pb, Zn) through Orontes River. Monitoring study results belonging to 5 different stations in Orontes River was obtained from State of Hydraulic Works and evaluated according to Box and Whisker plots, two-way PERMONAVA analysis and Mann-Kendall trend test. Results indicated that heavy metal concentrations has been increasing year by year. However, there is no significant spatial change in heavy metal concentration and rather homogenous distribution was observed through Orontes River. On the other hand, Iron (Fe) concentration in river was seasonal depended with no significant trend.
Afficher plus [+] Moins [-]Türkiye’de Yetiştirilen Holştayn İneklerde Döl Verim Özelliklerine Ait Fenotipik ve Genotipik Parametre Tahminleri
2017
Ahmet Derviş Sarar | İbrahim Tapkı
Bu araştırmada, Koçaş Tarım İşletmesinde yetiştirilen Siyah Alaca ineklerin döl verimi özellikleri incelenmiştir. Bu özelliklere ait fenotipik ve genotipik parametre tahminleri yapılmıştır. İncelenen özelliklere ait en küçük kareler ortalaması ilkine damızlıkta kullanma yaşı (İDKY), ilkine buzağılama yaşı (İBY), buzağılama aralığı (BA), gebelik başına tohumlama sayısı (GBTS) ve servis periyodu (SP) için sırasıyla; 529,66, 849,59, 378,61 gün, 1,92 adet ve 105,67 gün olarak tespit edilmiştir. Yine aynı özelliklere ait kalıtım dereceleri ise sırasıyla; 0,30, 0,20, 0,02, 0,09 ve 0,11 olarak tahmin edilmiştir. Yılın, mevsimin ve laktasyon sırasının gebelik başına tohumlama sayısı üzerine etkisi, buzağılama aralığı ve ilkine damızlıkta kullanma yaşı üzerine yılın etkisi önemli bulunmuştur. Araştırma sonuçları, süt verim özellikleri bakımından Koçaş Tarım İşletmesi yetiştiricilik şartlarının iyi durumda ve işletme için hesaplanan fenotipik ve genotipik parametrelerin de kabul edilebilir sınırlar içerisinde olduğunu göstermiştir.
Afficher plus [+] Moins [-]Effects of Kefir as a Probiotic Source on the Performance and Health of Young Dairy Calves
2017
Sema Satık | Mevlüt günal
The aim of this study was to investigate the effect of kefir as a probiotic on the performance and health status of calves. Thirty Holstein female calves with 3-day-old were randomly allocated to three treatment groups: Control (without any probiotic), probiotic (a commercial probiotic mixture-3 g/d/calf bacteria-based and 2 g/d/calf yeast-based) and kefir (20 ml/d/calf). The calves were weaned at 56 days of age. The experiment was performed in 70 days. Treatment had no effect (P>0.05) on weaning and final body weight and starter intake. Although differences in weight gain were not significant (P>0.05), there were trend to increase by probiotic treatments during 0-14 days. Probiotic treatments tended to have a positive effect on the population of the fecal lactic acid bacteria at 14 days. The results of the study indicated that kefir as a natural probiotic in calf nutrition may be beneficial during the first weeks of life.
Afficher plus [+] Moins [-]Effects of Harvest Times on the Fatty Acids Composition of Rose Hip (Rosa sp.) Seeds
2017
Mehmet Güneş | Ümit Dölek | Mahfuz Elmastaş | Fatma Karagöz
This study was conducted to determine the change of fatty acids ratios of some rose hip species seeds in different harvest times. Seeds of five genotypes belonging to rose hip species (Rosa sp.) were used in the study. The fruits of species were harvested in six different ripening times and analyzed. Total oil analysis was performed for the fifth harvest only, which was determined as the optimal harvest time. As a result; total oil ratio of rose hip seeds varied as 5.22 and 6.62 g/100g respectively for accessions of Rosa dumalis (MR-12 and MR-15), 6.37 g/100g for R. canina (MR-26), 5.00 g/100g for R. dumalis ssp. boissieri (MR-46) and 5.29 g/100g for R. villosa (MR-84). Eleven fatty acids were determined in rose hip seeds. Among these fatty acids linoleic, oleic, linolenic, palmitic and stearic acids respectively had high ratio. Saturated fatty acids ratio (SFAs) was the highest in R. canina (MR-26) and the lowest in R. dumalis (MR-12); monounsaturated fatty acids ratio (MUFAs) was the highest in R. dumalis (MR-12) and the lowest in R. dumalis ssp. boissieri (MR-46); polyunsaturated fatty acids ratio (PUFAs) was the highest in R. dumalis ssp. boissieri (MR-46) and the lowest in R. dumalis (MR-12). Mono and polyunsaturated fatty acid contents obtained in this study was high; the change of fatty acid profile in the studied species in relation to harvest time was significant for some species and insignificant for others. A conclusion was reached that it is important to pay attention to qualitative and quantitative properties of seeds when conducting studies about rose hip improvement.
Afficher plus [+] Moins [-]Effects of Some Environmental Factors on Milking Time Milk Yield in Red Holstein Cows
2017
Atakan Koc
The purpose of this study is to investigate the environmental factors on the milking time milk yield of Red Holstein cows. For this aim, 172,826 morning milk yield (MMY) and 172,771 evening milk yield (EMY) data collected between 2001 and 2010 from a dairy farm in Aydin Province, Turkey were used. The THI is over 72 from June to August in the region. The effects of calving month, calving year, parity, lactation month and milking month on MMY and EMY were found to be statistically significant. For every month, year and parity, the MMY means were higher than those of the EMY. The peak daily milk yield was determined on the 46th day of lactation (31.7±0.013 kg). For parity, the highest milk yield means were detected for the third parity: 13.69±0.023 kg for MMY and 11.70±0.021 kg for EMY. In conclusion, taking precautions to protect the cows from heat stress especially for the cows calved in summer and keeping the milking interval equal for whole year would help increasing the milk yield.
Afficher plus [+] Moins [-]Antibiotic Resistance Case Study: Enterobacteriaceae isolated from Batlama Creek in Giresun, Turkey
2017
Tamer Akkan
Enterobacteriaceae were isolated from the freshwater of the Batlama Creek in Giresun, to measure their resistance levels against to antibiotics. A total of 9 antibiotics disc were applied for the resistance test. Antibiotic resistances of all isolates were at percentages for ampicillin (75%), erythromycin (64%), nalidixic acid (48%), tetracycline (39%), amikacin (34%), cefazolin and chloramphenicol (33%), cefuroxime (32%) and cefotaxime (23%), respectively. The highest resistant strain was resistant against to 9 antibiotics, while the weak resistance of 16 isolates were sensitive to all antibiotics. Moreover, Multiple antibiotic resistance index values were found to be higher than 0.2 for 77% of all isolates. High resistances of examined bacteria against to antibiotics indicated a dense and multisource pollution in the Batlama Creek. Consequently, a need for good surveillance programs to monitor antimicrobial resistance patterns in surface water bodies.
Afficher plus [+] Moins [-]Antibacterial and Antioxidant Activities of Liquidambar Orientalis Mill. Various Extracts Against Bacterial Pathogens Causing Mastitis
2017
Gülten Ökmen | Zafer Cantekin | Mohammmad Intakhab Alam | Onur Türkcan | Yaşar Ergün
Antibiotic resistance is being constantly developed worldwide. Coagulase Negative Staphylococci (CNS) and Staphylococcus aureus are common causes of bovine subclinical mastitis. Bioactive compound of medicinal plants shows anti-microbial, anti-mutagenic and anti-oxidant effects. The anti-bacterial and anti-oxidant activities of Liquidambar orientalis (L. orientalis) extracts on subclinical mastitis causing bacteria in cows have not been reported to date. The aim of the present study was to examine anti-bacterial and anti-oxidant effects of L. orientalis leaf extracts on S. aureus and CNS isolated from cows with subclinical mastitis symptoms. In this study, 3.2 mg/mL minimum inhibitory concentration (MIC) of ethanol extracts of L. orientalis has shown to be a most potent anti-bacterial and anti-oxidant for all isolated bacterial species from mastitis cows. In this study, it was investigated anti-bacterial and anti-oxidant potentials of acetone, methanol and ethanol extracts of the L. orientalis. The acetone extract showed maximum inhibition zone against S. aureus numbered 17 (12 mm). In addition to anti-bacterial properties, anti-oxidant activity of L. orientalis extract was examined by ABTS [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] free radical assay. Trolox was used as a positive control anti-oxidant. Ethanol extract exhibited a strong anti-oxidant activity like Trolox anti-oxidant which was effective at 2.58 mM concentration. Bioactive compounds of sweet gum may be useful to screening mastitis causing bacteria for clinical applications.
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