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Seasonal variation of sperm kinematics in Murrah bulls under the tropical climatic condition Texte intégral
2021
Ranjana Sinha(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Livestock Production and Management) | Mukesh Bhakat(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Tushar Kumar Mohanty(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Raj Kumar(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Ashish Ranjan(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Animal Genetics and Breeding) | Abdul Rahim(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India)) | Shabir Ahmad Lone(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Nadeem Shah(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Adil Rasool Paray(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre) | Chandrashekhar Santosh Patil(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India)) | Ajeet Singh(National Dairy Research Institute, Karnal (India). Indian Council of Agricultural Research. Artificial Breeding Research Centre)
The present study was undertaken to identify the difference in sperm kinematics in Murrah buffalo bulls in different seasons. Eight Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-NDRI Karnal. Frozen semen samples of the bulls were obtained and sperm kinematic parameters were measured by computer-assisted sperm analyzer. The sperm kinematic parameters included curvilinear velocity (VCL), linear velocity (VSL), average path velocity (VAP), distance of average path (DAP), distance straight line (DSL), straightness coefficient (STR), linearity (LIN), amplitude of lateral head displacement (ALH), and beat cross frequency (BCF). Morphological abnormality of sperm includes bent tail (BT), coiled tail (CT), distal droplets (DD) and distal mid-piece reflex (DMR). Analysis revealed that the proportion of BT was significantly (P LT 0.05) lower in winter and autumn season as compared to summer and rainy season. Percent of total motile and progressive motile sperm was significantly (P LT 0.05) higher in winter season as compared to summer and rainy season. The VAP, VSL and VCL of sperm motion were significantly (P LT 0.05) higher in winter and autumn season as compared to the summer season. The DAP and STR were significantly (P LT 0.05) higher in winter and autumn season as compared to summer and rainy season. DSL and LIN were significantly (P LT 0.05) lower in summer and rainy season followed by autumn and winter season. Post-thaw motility and motion parameter varied significantly between seasons, and higher sperm motility was maintained in semen cryopreserved in winter followed by autumn, rainy and summer season.
Afficher plus [+] Moins [-]Effect of cholesterol loaded cyclodextrin on cryosurvivability of buffalo spermatozoa Texte intégral
2021
Rohit Bishist(Dr. YS Parmar University of Horticulture and Forestry Nauni, Himachal Pradesh (India). Veterinary Sciences) | Virendra Swarup Raina(Dr. YS Parmar University of Horticulture and Forestry Nauni, Himachal Pradesh (India). Veterinary Sciences) | Mukesh Bhakat(National Dairy Research Institute, Haryana (India). Indian Council of Agricultural Research. Artificial Breeding Research Center (ABRC)) | Shabir Ahmad Lone(Dr. YS Parmar University of Horticulture and Forestry Nauni, Himachal Pradesh (India). Veterinary Sciences) | Tushar Kumar Mohanty(Dr. YS Parmar University of Horticulture and Forestry Nauni, Himachal Pradesh (India). Veterinary Sciences) | Ranjana Sinha(Dr. YS Parmar University of Horticulture and Forestry Nauni, Himachal Pradesh (India). Veterinary Sciences) | Raj Kumar(National Dairy Research Institute, Haryana (India). Indian Council of Agricultural Research. Artificial Breeding Research Center (ABRC))
The study was carried out to understand the effect of various concentrations of cholesterol loaded cyclodextrin (CLC) on cryosurvivability of buffalo spermatozoa. Twenty four ejaculates with mass motility GT= 3+ from 4 bulls (6 from each bull) were collected as per standard procedure. Each ejaculate was split into four groups viz., Group I (control: extended with Tris-egg yolk-glycerol extender upto 80*106 sperm/ml), Group II (treated with CLC 1 mg/ml), Group III (treated with CLC 2 mg/ml) and Group IV (treated with CLC 3 mg/ml). Semen samples of Group II, III or IV were incubated with CLC at 37 deg C for 15 minutes for the entry of cholesterol into sperms and finally diluted upto 80*106 sperm/ml. The results revealed that post-dilution and after freezing at 0.7 and 30 days of cryopreservation motility, live and dead, acrosomal integrity and hypo-osmotic swelling test were significantly (P LT 0.05) higher in Group III as compared to all other groups. However, significantly (P LT 0.05) higher percentage of abnormal sperm was observed in control group as compared to other groups. Therefore, addition of CLC 2 mg/ml in extender during buffalo semen cryopreservation has beneficial role in improvement of semen quality.
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