The present study was designed to assess the bacterial isolation and their antibiogram from endometritis in slaughtered buffaloes. A total 110 uterine swabs at horn-body junction of slaughtered buffaloes were collected from local abattoir in Junagadh. Out of 110 uterine swabs 56 (50.90 percent) uterine samples showed the growth of various bacteria and remaining 54 swabs (49.10 percent) were found to be sterile. Among 56 uterine samples 50 swabs (89.28 percent) showed single isolate and remaining 06 (10.72 percent) exhibited mixed infection. The Escherichia coli and Staphylococcus spp. isolates (24.19 percent) top the list followed by Corynebacterium spp. (17.74 percent), Micrococcus spp. (14.52 percent), Fusobacterium spp. (8.06 percent), Pseudomonas spp. (4.84 percent), Bacillus spp. (4.84 percent) and Streptococcus spp. (1.61 percent). The antimicrobial susceptibility of these bacterial isolates were showed highest sensitivity against Chloramphenicol (83.9 percent) followed by Gentamicin (80.6 percent), Levofloxacin (77.4 percent), Oxytetracycline (77.4 percent), Ceftriaxone/Sulbactam (69.3 percent), Cefoperazone/Sulbactam (61.2 percent) and Amoxicillin/Sulbactam (33.9 percent).

The Present study was conducted to retrospectively analyze the pattern of calf mortality (0 to 1 year age) from 1992 to 1993 to 2015 to 2016 in an organized Murrah buffalo herd maintained at Central Institute for Research on Buffaloes (CIRB), Hisar, Haryana. During the study period, highest calf mortality (8.35 percent) was observed inperiod P1 (1992 to 1995), while lowest mortality (2.64 percent) was observed in P7 (2010 to 2013) with an overall mortality rate of 4.94 percent from1992 1993 to 2015 to 2016 over the period of twenty five years.The overallage-specific mortality (9.5 percent) was observed highest in Group I: birth to three months age,while lowest mortality (2.5 percent) was observed in Group 3: 6 to 12 months age. There was no significant difference in overall sex-specific mortality (P LT= 0.01) over the period of twenty four years. Highest proportionate mortality rate (37.6 percent) was observed during winter season and greatest losses in terms of proportionate mortality rate (30.7 percent) were attributed to gastrointestinal plus pneumonia causes of mortality. Chi square test depicted statistically significant association of period, age group, season and cause of deathwith the calf mortality at P LT = 0.01. Retrospective investigation indicates that overall calf mortality rate from birth to one year of age was much lesser during the recent period (P7: 2.6 percent, P8: 3.1 percent) possibly attributed to good husbandry conditions including routine vaccination schedule, optimum colostrum feeding and improved nutritional management as contributing factors here for a steady improvement in the management and health status of calves through time. Based on findings of the highest mortality up to 3 months age (9.5 percent) in the present study, proper hygienic conditions in calf sheds, adequate healthcare, appropriate colostrum feeding and good husbandry conditions during this critical period are recommended to minimise the calf exposure to new infections.

This research examined the effects of storage temperature and storage time on the somatic cell count (SCC) of milk from Anatolian buffaloes, which was measured with the DeLaval cell counter (DCC). Storage temperature and time are among the different factors that potentially affect the SCC of Anatolian buffalo milk. In this context, 20 milk samples were collected from Anatolian Buffaloes and analyzed. The milk samples were divided into two groups according to their measured level of SCC. These two groups were the low score (LT= 3.16 cell/ml) group and the high score (GT 3.16 cell/ml) group. The mean logSCC values of the low score and the high score groups were determined as 2.27+-0.045 and 4.06+-0.019 cells/ml, respectively. In this research, the effects of storage temperature (4 deg C, 21 deg C) and storage time (fresh milk, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and 24 h) on logSCC were determined to be statistically significant (P LT 0.01). Thus, increases in storage temperature and storage time were associated with an increase in the logSCC of the milk samples. In this study, the effects of storage temperature (4 deg C, 21 deg C) and storage time (fresh milk, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and 24 hours) on logSCC were determined to be statistically significant (P LT 0.01). Thus, increases in storage temperature and storage time were associated with an increase in the logSCC of the milk samples.

The ability to utilize low-quality roughage of swamp buffalo has been reported, thus, rumen of swamp buffalo might be a suitable source for isolation of potent fibrolytic bacteria. A total of 165 gram-negative cocci fibrolytic bacteria were screened from rumen contents of 4 swamp buffalo. Six isolates were identified as Ruminococcus flavefaciens and phylogenetically grouped with type strain. Strain OS14 showed the highest fiber digestion alone or combination with non-fibrolytic bacteria and higher than type strain. DM digestibility of cellulose powder in co-culture of OS14 with all strains non-fibrolytic was increased. Moreover, co-culture of OS14 with S137 showed the highest fiber digestion and notably increased of acetate and propionate. These results indicated that possible cross-feeding between R. flavefaciens with non-fibrolytic bacteria could improve fiber digestion but the amount of improvement might be based on the combination of bacteria strains. The in vitro fermentation products and abundance in co-culture need to be quantified in further study.

Buffalo milk is the most preferred commodity in Pakistan due to high fat contents. Hydrolysis of circulating triglycerides and uptake of fatty acids in the mammary gland is regulated by lipoprotein lipase (LPL). This study is designed to identify single nucleotide polymorphisms (SNPs) in LPL gene among high and low milk producing buffalo breeds of Pakistan. We selected samples (n=50) of each Nili-Ravi a high milk producing and Azakheli a low milk producing buffalo breeds. Blood samples were collected for DNA extraction. LPL region of exon 2 region along with exon/intron boundaries were sequenced and data was analyzed for variation detection. Allele frequency was calculated using Hardy-Weinberg equation and in-silico analysis was performed for functional prediction and genetic diversity assessment. We found one single nucleotide polymorphism c.258+43C GT T in the intronic region of LPL gene. This polymorphism followed the Hardy-Weinberg equilibrium in both Nili-Ravi (P-value = 0.538) and Azakheli (P-value = 0.077). Association of T-allele analysis predicted it significantly higher (P-value = 0.009) in Nili-Ravi. We found one novel SNP (c.258+43C GT T) in the LPL gene in both high and low milk producing buffalo breeds of Pakistan but high in Nili-Ravi. Lower splice site effect suggests its less strength of association with milk producing trait.

This article details about successful management of prolong gestation associated with partial mummification in buffalo.

Nine buffaloes non-descript aged between 4 to 9 years affected by onion toxicosis were presented to Veterinary College, Hospital, Bidar, with the signs of anorexia, dullness, depression, staggering gait and passing dark brown coloured urine. On clinical examination affected buffaloes revealed tachycardia, polypnoea, pale mucous membrane, and coffee decoction color urine. Hematological examination revealed decreased values of total erythrocyte count, hemoglobin concentration and packed cell volume with leukocytosis. Erythrocyte indices revealed tendency towards macrocytic hypochromic anemia. Serum biochemistry revealed normal serum urea nitrogen and total serum bilirubin. Affected buffaloes were treated with vitamin E and Selenium along with supportive therapy of 5 percent dextrose and 8 buffaloes were recovered within 5 days of treatment. Necropsy findings of dead buffalo revealed pallor of tissue, jaundice, dark brown kidneys and urine and fragments of onion in rumen.

Vaginal cytology is a simple technique that can be used to determine the stages of the estrus cycle, if there are pathological conditions of the reproductive tract, and the optimal time of mating. There have been many vaginal cytology studies and it's clinical applications have been reported in many species. There have been few reports, however, about its use in buffalo. Buffalo are considered poor breeders as their reproductive efficiency is adversely affected by certain limitations such as: late maturity, seasonality of breeding, silent heat coupled with poor expression of estrus, low conception rates and long intercalving period. Accurate heat detection is one of the key factors to increase the conception rate. Vaginal cytology in combination with other heat detection methods can help in this process. The objective of this review article is to focus on the use of the vaginal cytology technique for estrus detection and other applications in buffalo.

The objectives of the study were to evaluate the ultrasonographic morphometry of the reticulum in healthy non-gravid cattle and buffaloes from right and left parasternal and lateral windows and to evaluate the role of ultrasonography in the prediction of penetrating metallic foreign bodies in the reticular wall in cattle and buffaloes. The study included 22 clinically healthy (11 cross-bred Indian cattle (Bos tarus and Bos indicus) and 11 Indian water buffaloes (Bubalus bubalis)) and 26 traumatic reticulitis affected bovines (21 cattle and 15 buffaloes). Various parameters like, reticular wall thickness, depth of reticulum, pattern of reticular motility and wall, and presence of effusions were recorded to assess the penetrability of metallic sharp foreign body using ultrasonography.The reticular wall thickness varied from 0.34 to 0.82 cm and 0.37 to 0.68 cm in healthy cattle and buffaloes, respectively. In both the species, the mean highest reticular wall thickness was recorded on the left lateral side. Instead of typical biphasic motility, folding type motility was recorded in 72.73 percent healthy buffaloes and 27.27 percent healthy cattle from the left lateral side. The reticular wall pattern was recorded to be smoother in healthy buffaloes from all the windows compared to healthy cattle. The peri-reticular reaction in diseased bovine was least observed on the left lateral aspect of the reticulum and was maximum evident on the left ventral aspect of reticulum in cattle and the right ventral aspect in buffaloes. The reticular motility was present in maximum number of bovine despite adhesions present on rumenotomy. The cattle showed more adhesions for partially penetrating foreign bodies, compared to buffaloes. Despite non-penetrating foreign bodies, the peri-reticular effusions were seen on the ventral aspect of reticulum from both sides, though in less quantity. In conclusion, the peri-reticular effusions are maximum seen on the left ventral aspect in cattle and right ventral in buffalo in completely and partially penetrating foreign bodies; however, effusions may also be present in non-penetrating foreign body in cattle.

The histomorphological studies on ontogeny of Peyer's patches in jejunum of 20 buffalo fetuses ranging from 11.5 cm CVRL (80 days) to 100 cm CVRL (299 days) were conducted. The fetuses were categorized into three groups based on their curved crown rump length (CVRL). The fetuses of gestational age from 11.5 cm CVRL (80 days) to 28 cm CVRL (136 days) were devoid of typical jejunal lymphoid patches. However, at 32 cm CVRL (145 days) aggregates of 2 to 3 lymphocytes were observed arranged linearly in submucosa of jejunum Elongated to oval lymphocytic aggregations were observed in submucosa at 54 cm CVRL (195 days) that formed primordia of lymphoid follicle. The presence of darkly stained lymphocytes was also observed in the intestinal villi above the aggregates at this stage. At 70 cm CVRL (231 days), a number of round, pear shaped developing lymphoid follicles were encountered. At 100 cm CVRL (full term), completely developed lymphoid follicles of different shapes i.e., oval, pear shaped and square shaped follicles were present that were arranged in a single row on the anti-mesenteric part of jejunum. The dome of lymphoid follicle was completely formed at this age by invading the jejunal villi that formed arcs over it. Therefore, the present study suggests that the jejunal Peyer's patches started its development at mid-gestational age and become completely developed in the fetuses that reached upto full term just before birth. Thus these jejunal Peyer's patches generates immune response by sampling foreign antigens entering the lumen and play a crucial role in terms of generating mucosal immunity.