Case reports are extremely valuable in forensic entomology and very rare in Brazil. In this report we describe a case of multiple colonization of a preserved male cadaver found indoors in Santa Catarina State, southern Brazil, by four dipterans species of four different families: Fannia canicularis (Linnaeus, 1761) (Diptera: Fanniidae), Microcerella halli (Engel, 1931) (Diptera: Sarcophagidae), Muscina stabulans (Fallén, 1817) (Diptera: Muscidae) and Sarconesia chlorogaster (Wiedemann, 1830) (Diptera: Calliphoridae). The development time data of the species were used to estimate the minimum postmortem interval (PMI). Considering the methodology applied in this study and the values calculated for the species development, it was possible to estimate a minimum PMI of 24 days. Besides the diversity of dipteran species colonizing a single human body in an indoor environment, this case report reinforces the importance of these species as forensically indicator to estimate the time of death.

Este estudo foi realizado entre abril e setembro de 2008 e relata a ocorrência de miíases humanas causadas por Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) em São Gonçalo no estado do Rio de Janeiro, Brasil. Para a coleta das larvas, foi utilizada vaselina líquida ou sólida para sufocar as larvas, que foram então preservadas em etanol 70% e enviadas para o Instituto Oswaldo Cruz para identificação. As larvas provenientes de três pacientes parasitados, um homem e duas mulheres negros com idade entre 36 a 76 anos, foram identificadas como pertencentes a espécie C. hominivorax. Ferida aberta foi a principal causa da parasitose, e ainda higiene pessoal precária, baixo nível educacional, alcoolismo e paciente acamado foram possivelmente os fatores secundários. Human Myiasis Caused by Larvae of Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) in São Gonçalo, RJ, Brazil: Socio-Economic Approach Abstract. This study was carried out between April and September 2008 and reports on the occurrence of human myiasis caused by the New World screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) in São Gonçalo in the state of Rio de Janeiro, Brazil. Liquid or solid vaseline was used to suffocate the larvae, which were then preserved in 70% ethanol and sent to the Instituto Oswaldo Cruz for identification. There were 01 male and 02 females black patients with ages ranging from 36 to 76 and C. hominivorax were identified in all 03 cases of myiasis. Open wounds were the main cause of the parasitosis, whereas poor personal hygiene, the low educational level, alcoholism, bedridden patients were possibly secondary factors.

Este estudo foi realizado entre abril e setembro de 2008 e relata a ocorrência de miíases humanas causadas por Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) em São Gonçalo no estado do Rio de Janeiro, Brasil. Para a coleta das larvas, foi utilizada vaselina líquida ou sólida para sufocar as larvas, que foram então preservadas em etanol 70% e enviadas para o Instituto Oswaldo Cruz para identificação. As larvas provenientes de três pacientes parasitados, um homem e duas mulheres negros com idade entre 36 a 76 anos, foram identificadas como pertencentes a espécie C. hominivorax. Ferida aberta foi a principal causa da parasitose, e ainda higiene pessoal precária, baixo nível educacional, alcoolismo e paciente acamado foram possivelmente os fatores secundários. Human Myiasis Caused by Larvae of Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) in São Gonçalo, RJ, Brazil: Socio-Economic Approach Abstract. This study was carried out between April and September 2008 and reports on the occurrence of human myiasis caused by the New World screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) in São Gonçalo in the state of Rio de Janeiro, Brazil. Liquid or solid vaseline was used to suffocate the larvae, which were then preserved in 70% ethanol and sent to the Instituto Oswaldo Cruz for identification. There were 01 male and 02 females black patients with ages ranging from 36 to 76 and C. hominivorax were identified in all 03 cases of myiasis. Open wounds were the main cause of the parasitosis, whereas poor personal hygiene, the low educational level, alcoholism, bedridden patients were possibly secondary factors.

Este trabalho teve como objetivo contribuir com o conhecimento da entomofauna de Calliphoridae (Diptera) no município de Itaboraí, RJ, Brasil e quantificar as espécies mais predominantes de importância sanitária. As moscas foram capturadas em oito diferentes pontos no período de um ano, usando sempre isca de peixe. Após triagem, as espécies foram separadas por espécie e inseridas na coleção entomológica do Laboratório de Transmissores de Leishmaniose (Setor de Entomologia Médica e Forense) do Instituto Oswaldo Cruz - IOC/FIOCRUZ. Foram capturadas 1792 moscas pertencentes a sete (7) espécies da família Calliphoridae: Chloroprocta idioidea (Robineau-Desvoidy) (0,11%), Chrysomya megacephala (Fabricius) (87,94%), Chrysomya albiceps (Wiedemann) (6,70%), Chrysomya putoria (Wiedemann) (1,23%), Cochliomyia macellaria (Fabricius) (0,56%), Hemilucilia segmentaria (Fabricius) (0,33%), Lucilia eximia (Wiedemann) (3,13%). Occurrence and seasonality of muscoid (Diptera, Calliphoridae) of public health importance in Itaboraí (RJ), Brazil Abstract. This work was carried out to contribute to the knowledge of Calliphoridae flies (Diptera) in Itaboraí, RJ, Brazil and quantify the predominant species of health importance. The flies were captured in eight different points in the city over a one year period, always using fish as bait, separated by species and kept properly in an entomological box in the Laboratório de Transmissores de Leishmaniose (Setor de Entomologia Médica e Forense) - IOC / FIOCRUZ, RJ. A total of 1792 Calliphoridae flies were captured, belonging to seven (7) species: Chloroprocta idioidea (Robineau-Desvoidy) (0.11%), Chrysomya megacephala (Fabricius) (87.94%), Chrysomya albiceps (Wiedemann) (6.70%), Chrysomya putoria (Wiedemann) (1.23%), Cochliomyia macellaria (Fabricius) (0.56%), Hemilucilia segmentaria (Fabricius) (0.33%), Lucilia eximia (Wiedemann) (3.13%).

Este trabalho teve como objetivo contribuir com o conhecimento da entomofauna de Calliphoridae (Diptera) no município de Itaboraí, RJ, Brasil e quantificar as espécies mais predominantes de importância sanitária. As moscas foram capturadas em oito diferentes pontos no período de um ano, usando sempre isca de peixe. Após triagem, as espécies foram separadas por espécie e inseridas na coleção entomológica do Laboratório de Transmissores de Leishmaniose (Setor de Entomologia Médica e Forense) do Instituto Oswaldo Cruz - IOC/FIOCRUZ. Foram capturadas 1792 moscas pertencentes a sete (7) espécies da família Calliphoridae: Chloroprocta idioidea (Robineau-Desvoidy) (0,11%), Chrysomya megacephala (Fabricius) (87,94%), Chrysomya albiceps (Wiedemann) (6,70%), Chrysomya putoria (Wiedemann) (1,23%), Cochliomyia macellaria (Fabricius) (0,56%), Hemilucilia segmentaria (Fabricius) (0,33%), Lucilia eximia (Wiedemann) (3,13%). Occurrence and seasonality of muscoid (Diptera, Calliphoridae) of public health importance in Itaboraí (RJ), Brazil Abstract. This work was carried out to contribute to the knowledge of Calliphoridae flies (Diptera) in Itaboraí, RJ, Brazil and quantify the predominant species of health importance. The flies were captured in eight different points in the city over a one year period, always using fish as bait, separated by species and kept properly in an entomological box in the Laboratório de Transmissores de Leishmaniose (Setor de Entomologia Médica e Forense) - IOC / FIOCRUZ, RJ. A total of 1792 Calliphoridae flies were captured, belonging to seven (7) species: Chloroprocta idioidea (Robineau-Desvoidy) (0.11%), Chrysomya megacephala (Fabricius) (87.94%), Chrysomya albiceps (Wiedemann) (6.70%), Chrysomya putoria (Wiedemann) (1.23%), Cochliomyia macellaria (Fabricius) (0.56%), Hemilucilia segmentaria (Fabricius) (0.33%), Lucilia eximia (Wiedemann) (3.13%).

Este trabalho teve como objetivo contribuir com o conhecimento da entomofauna de Calliphoridae (Diptera) no município de Itaboraí, RJ, Brasil e quantificar as espécies mais predominantes de importância sanitária. As moscas foram capturadas em oito diferentes pontos no período de um ano, usando sempre isca de peixe. Após triagem, as espécies foram separadas por espécie e inseridas na coleção entomológica do Laboratório de Transmissores de Leishmaniose (Setor de Entomologia Médica e Forense) do Instituto Oswaldo Cruz - IOC/FIOCRUZ. Foram capturadas 1792 moscas pertencentes a sete (7) espécies da família Calliphoridae: Chloroprocta idioidea (Robineau-Desvoidy) (0,11%), Chrysomya megacephala (Fabricius) (87,94%), Chrysomya albiceps (Wiedemann) (6,70%), Chrysomya putoria (Wiedemann) (1,23%), Cochliomyia macellaria (Fabricius) (0,56%), Hemilucilia segmentaria (Fabricius) (0,33%), Lucilia eximia (Wiedemann) (3,13%).Occurrence and seasonality of muscoid (Diptera, Calliphoridae) of public healthimportance in Itaboraí (RJ), BrazilAbstract. This work was carried out to contribute to the knowledge of Calliphoridae flies (Diptera) in Itaboraí, RJ, Brazil and quantify the predominant species of health importance. The flies were captured in eight different points in the city over a one year period, always using fish as bait, separated by species and kept properly in an entomological box in the Laboratório de Transmissores de Leishmaniose (Setor de Entomologia Médica e Forense) - IOC / FIOCRUZ, RJ. A total of 1792 Calliphoridae flies were captured, belonging to seven (7) species: Chloroprocta idioidea (Robineau-Desvoidy) (0.11%), Chrysomya megacephala (Fabricius) (87.94%), Chrysomya albiceps (Wiedemann) (6.70%), Chrysomya putoria (Wiedemann) (1.23%), Cochliomyia macellaria (Fabricius) (0.56%), Hemilucilia segmentaria (Fabricius) (0.33%), Lucilia eximia (Wiedemann) (3.13%).

Forensic entomology uses insects, particularly Diptera, as evidence in various forensic applications. Some species help estimate post-mortem intervals in criminal investigations, others indicate cases of neglect by signaling poor hygiene conditions, and some serve as bioindicators of environmental quality. Characterizing local fly species distributions is essential for forensic applications, such as post-mortem interval estimation. In Belo Horizonte, a city in Minas Gerais state, Brazil, only one prior study has been conducted, solely focusing on Calliphoridae species while overlooking other families, such as Fanniidae, Muscidae, and Sarcophagidae, which are highly relevant for forensic applications. To address this gap, we collected flies of potential forensic interest at the Ecological Station of "Universidade Federal de Minas Gerais" (EEco UFMG), using fresh beef as bait, during the warm and rainy season. A total of 4,612 flies were collected, with Calliphoridae (N = 3024; 65.5%), Fanniidae (N = 902; 19.5%), Sarcophagidae (N = 353; 8.0%), and Muscidae (N = 240; 5.0%), constituting 98.0% of the collected specimens. The remaining 2% (N = 93) of the specimens belong to 11 families. Thirty-nine species were identified, with Muscidae exhibiting the highest richness (15 species), followed by Sarcophagidae (10), Calliphoridae (9), and Fanniidae (4). Notably, six new geographic records for Minas Gerais state and three for Belo Horizonte city were documented within the Muscidae family. Checklists of fly species of potential forensic interest from unexplored locations, such as the EEco UFMG, contribute to the development of valuable databases for future applications in forensic entomology.

Este estudo foi realizado entre abril e setembro de 2008 e relata a ocorrência de miíases humanas causadas por Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) em São Gonçalo no estado do Rio de Janeiro, Brasil. Para a coleta das larvas, foi utilizada vaselina líquida ou sólida para sufocar as larvas, que foram então preservadas em etanol 70% e enviadas para o Instituto Oswaldo Cruz para identificação. As larvas provenientes de três pacientes parasitados, um homem e duas mulheres negros com idade entre 36 a 76 anos, foram identificadas como pertencentes a espécie C. hominivorax. Ferida aberta foi a principal causa da parasitose, e ainda higiene pessoal precária, baixo nível educacional, alcoolismo e paciente acamado foram possivelmente os fatores secundários. Human Myiasis Caused by Larvae of Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) in São Gonçalo, RJ, Brazil: Socio-Economic Approach Abstract. This study was carried out between April and September 2008 and reports on the occurrence of human myiasis caused by the New World screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) in São Gonçalo in the state of Rio de Janeiro, Brazil. Liquid or solid vaseline was used to suffocate the larvae, which were then preserved in 70% ethanol and sent to the Instituto Oswaldo Cruz for identification. There were 01 male and 02 females black patients with ages ranging from 36 to 76 and C. hominivorax were identified in all 03 cases of myiasis. Open wounds were the main cause of the parasitosis, whereas poor personal hygiene, the low educational level, alcoholism, bedridden patients were possibly secondary factors.